Chlamydomonas reinhardtii (Chlamydomonas smithii)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Chlamydomonadales; Chlamydomonadaceae;

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K3DH94|A0A2K3DH94_CHLRE Isoform of A0A2K3DH64 Sox C-terminal domain-containing protein OS=Chlamydomonas reinhardtii OX=3055 GN=CHLRE_08g370750v5 PE=3 SV=1
MM1 pKa = 7.23QLTAKK6 pKa = 10.23KK7 pKa = 10.5LLINDD12 pKa = 4.3FVTRR16 pKa = 11.84NGGSLVANTQGGFGSAGFGFFPLSLTYY43 pKa = 10.71VPADD47 pKa = 3.1TSGFQNIFQVQPDD60 pKa = 3.83ATDD63 pKa = 2.9LWGSGFTDD71 pKa = 3.32AALAAGPWHH80 pKa = 6.93GYY82 pKa = 4.84WTGPSGWLGLRR93 pKa = 11.84PLVARR98 pKa = 11.84TGSCPVPLPTGTTAQDD114 pKa = 3.54CQAAALWGKK123 pKa = 9.28KK124 pKa = 8.55VKK126 pKa = 9.1LTAEE130 pKa = 3.87ICNNGIDD137 pKa = 4.4DD138 pKa = 5.17DD139 pKa = 5.24GDD141 pKa = 3.9GLVDD145 pKa = 3.78GGVNGDD151 pKa = 3.88PDD153 pKa = 3.77CWRR156 pKa = 11.84CGNGIIDD163 pKa = 3.95PCEE166 pKa = 4.27DD167 pKa = 4.2CDD169 pKa = 5.33DD170 pKa = 4.26GNITGGDD177 pKa = 3.75GCSSQCLLEE186 pKa = 4.18FF187 pKa = 4.91

Molecular weight: 19.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K3DFR7|A0A2K3DFR7_CHLRE GIY-YIG domain-containing protein OS=Chlamydomonas reinhardtii OX=3055 GN=CHLRE_09g412700v5 PE=4 SV=1
MM1 pKa = 7.64PRR3 pKa = 11.84PPPSATGRR11 pKa = 11.84ARR13 pKa = 11.84PAAPSGSAPGALPSTGAGRR32 pKa = 11.84GRR34 pKa = 11.84GRR36 pKa = 11.84GAHH39 pKa = 6.94RR40 pKa = 11.84GRR42 pKa = 11.84GSKK45 pKa = 9.67PRR47 pKa = 11.84AAAPAAAPVPLPSGRR62 pKa = 11.84GSGRR66 pKa = 11.84GRR68 pKa = 11.84GAPQPSAPPSVTTRR82 pKa = 11.84SRR84 pKa = 11.84ARR86 pKa = 11.84ATRR89 pKa = 11.84APP91 pKa = 3.59

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17614

1218

18832

14029357

31

23859

745.0

76.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

17.142 ± 0.054

1.321 ± 0.008

4.223 ± 0.013

4.844 ± 0.021

2.015 ± 0.01

11.474 ± 0.041

2.119 ± 0.011

1.948 ± 0.01

2.415 ± 0.015

8.616 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.801 ± 0.007

1.952 ± 0.01

7.732 ± 0.068

4.646 ± 0.021

6.23 ± 0.018

7.772 ± 0.028

4.754 ± 0.013

6.137 ± 0.014

1.16 ± 0.006

1.696 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski