Amino acid dipepetide frequency for Gordonia phage Kita

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.086AlaAla: 19.086 ± 2.253
0.906AlaCys: 0.906 ± 0.3
8.576AlaAsp: 8.576 ± 0.779
7.972AlaGlu: 7.972 ± 0.675
2.355AlaPhe: 2.355 ± 0.472
9.422AlaGly: 9.422 ± 0.771
1.872AlaHis: 1.872 ± 0.372
5.617AlaIle: 5.617 ± 0.574
4.167AlaLys: 4.167 ± 0.553
7.67AlaLeu: 7.67 ± 0.815
3.503AlaMet: 3.503 ± 0.521
3.261AlaAsn: 3.261 ± 0.443
5.194AlaPro: 5.194 ± 0.558
4.409AlaGln: 4.409 ± 0.758
7.127AlaArg: 7.127 ± 0.603
6.463AlaSer: 6.463 ± 0.791
8.637AlaThr: 8.637 ± 1.282
9.12AlaVal: 9.12 ± 0.908
1.812AlaTrp: 1.812 ± 0.34
2.537AlaTyr: 2.537 ± 0.346
0.0AlaXaa: 0.0 ± 0.0
Cys
0.966CysAla: 0.966 ± 0.269
0.06CysCys: 0.06 ± 0.069
0.664CysAsp: 0.664 ± 0.255
0.604CysGlu: 0.604 ± 0.25
0.121CysPhe: 0.121 ± 0.089
1.027CysGly: 1.027 ± 0.383
0.121CysHis: 0.121 ± 0.096
0.0CysIle: 0.0 ± 0.0
0.06CysLys: 0.06 ± 0.066
0.362CysLeu: 0.362 ± 0.163
0.181CysMet: 0.181 ± 0.115
0.302CysAsn: 0.302 ± 0.155
0.302CysPro: 0.302 ± 0.128
0.181CysGln: 0.181 ± 0.102
0.906CysArg: 0.906 ± 0.266
0.302CysSer: 0.302 ± 0.132
0.483CysThr: 0.483 ± 0.192
0.302CysVal: 0.302 ± 0.125
0.181CysTrp: 0.181 ± 0.095
0.423CysTyr: 0.423 ± 0.148
0.0CysXaa: 0.0 ± 0.0
Asp
7.248AspAla: 7.248 ± 0.851
0.544AspCys: 0.544 ± 0.235
6.704AspAsp: 6.704 ± 0.83
3.986AspGlu: 3.986 ± 0.468
1.691AspPhe: 1.691 ± 0.349
6.402AspGly: 6.402 ± 0.759
2.235AspHis: 2.235 ± 0.429
2.054AspIle: 2.054 ± 0.383
1.812AspLys: 1.812 ± 0.315
7.67AspLeu: 7.67 ± 0.651
1.329AspMet: 1.329 ± 0.25
2.476AspAsn: 2.476 ± 0.351
5.013AspPro: 5.013 ± 0.596
2.295AspGln: 2.295 ± 0.343
4.832AspArg: 4.832 ± 0.721
2.899AspSer: 2.899 ± 0.445
4.409AspThr: 4.409 ± 0.605
4.651AspVal: 4.651 ± 0.551
1.45AspTrp: 1.45 ± 0.28
1.268AspTyr: 1.268 ± 0.239
0.0AspXaa: 0.0 ± 0.0
Glu
6.342GluAla: 6.342 ± 0.892
0.664GluCys: 0.664 ± 0.232
2.899GluAsp: 2.899 ± 0.477
3.08GluGlu: 3.08 ± 0.479
2.295GluPhe: 2.295 ± 0.425
3.503GluGly: 3.503 ± 0.53
0.906GluHis: 0.906 ± 0.259
3.503GluIle: 3.503 ± 0.558
2.235GluLys: 2.235 ± 0.39
4.409GluLeu: 4.409 ± 0.563
0.906GluMet: 0.906 ± 0.204
1.57GluAsn: 1.57 ± 0.321
2.718GluPro: 2.718 ± 0.404
3.08GluGln: 3.08 ± 0.454
4.288GluArg: 4.288 ± 0.514
2.657GluSer: 2.657 ± 0.489
3.986GluThr: 3.986 ± 0.563
4.167GluVal: 4.167 ± 0.554
1.087GluTrp: 1.087 ± 0.245
0.906GluTyr: 0.906 ± 0.258
0.0GluXaa: 0.0 ± 0.0
Phe
3.02PheAla: 3.02 ± 0.393
0.121PheCys: 0.121 ± 0.096
2.416PheAsp: 2.416 ± 0.374
1.691PheGlu: 1.691 ± 0.371
0.544PhePhe: 0.544 ± 0.156
2.416PheGly: 2.416 ± 0.395
0.423PheHis: 0.423 ± 0.195
0.785PheIle: 0.785 ± 0.203
1.027PheLys: 1.027 ± 0.277
1.027PheLeu: 1.027 ± 0.271
0.423PheMet: 0.423 ± 0.14
1.148PheAsn: 1.148 ± 0.219
1.087PhePro: 1.087 ± 0.222
0.604PheGln: 0.604 ± 0.176
2.174PheArg: 2.174 ± 0.47
1.57PheSer: 1.57 ± 0.315
1.631PheThr: 1.631 ± 0.289
1.993PheVal: 1.993 ± 0.421
1.087PheTrp: 1.087 ± 0.228
0.604PheTyr: 0.604 ± 0.212
0.0PheXaa: 0.0 ± 0.0
Gly
8.033GlyAla: 8.033 ± 0.638
0.483GlyCys: 0.483 ± 0.289
4.59GlyAsp: 4.59 ± 0.546
3.805GlyGlu: 3.805 ± 0.461
2.235GlyPhe: 2.235 ± 0.337
7.852GlyGly: 7.852 ± 1.023
1.268GlyHis: 1.268 ± 0.263
3.986GlyIle: 3.986 ± 0.386
3.443GlyLys: 3.443 ± 0.409
5.979GlyLeu: 5.979 ± 0.703
1.933GlyMet: 1.933 ± 0.402
3.986GlyAsn: 3.986 ± 0.493
3.805GlyPro: 3.805 ± 0.477
3.805GlyGln: 3.805 ± 0.527
6.523GlyArg: 6.523 ± 0.755
5.375GlySer: 5.375 ± 0.664
6.583GlyThr: 6.583 ± 1.249
6.342GlyVal: 6.342 ± 0.647
1.389GlyTrp: 1.389 ± 0.431
2.054GlyTyr: 2.054 ± 0.298
0.0GlyXaa: 0.0 ± 0.0
His
1.631HisAla: 1.631 ± 0.279
0.181HisCys: 0.181 ± 0.113
1.45HisAsp: 1.45 ± 0.331
1.268HisGlu: 1.268 ± 0.305
0.544HisPhe: 0.544 ± 0.158
1.45HisGly: 1.45 ± 0.279
1.087HisHis: 1.087 ± 0.279
0.966HisIle: 0.966 ± 0.273
0.362HisLys: 0.362 ± 0.14
1.993HisLeu: 1.993 ± 0.42
0.181HisMet: 0.181 ± 0.109
0.544HisAsn: 0.544 ± 0.214
1.51HisPro: 1.51 ± 0.403
1.087HisGln: 1.087 ± 0.277
1.45HisArg: 1.45 ± 0.356
0.785HisSer: 0.785 ± 0.236
1.51HisThr: 1.51 ± 0.3
0.664HisVal: 0.664 ± 0.217
0.302HisTrp: 0.302 ± 0.154
0.785HisTyr: 0.785 ± 0.246
0.0HisXaa: 0.0 ± 0.0
Ile
5.798IleAla: 5.798 ± 0.673
0.483IleCys: 0.483 ± 0.171
4.409IleAsp: 4.409 ± 0.482
3.382IleGlu: 3.382 ± 0.426
0.664IlePhe: 0.664 ± 0.143
4.167IleGly: 4.167 ± 0.615
0.906IleHis: 0.906 ± 0.243
1.691IleIle: 1.691 ± 0.329
0.906IleLys: 0.906 ± 0.237
2.355IleLeu: 2.355 ± 0.44
0.362IleMet: 0.362 ± 0.167
1.148IleAsn: 1.148 ± 0.254
2.959IlePro: 2.959 ± 0.476
1.993IleGln: 1.993 ± 0.375
3.261IleArg: 3.261 ± 0.422
2.295IleSer: 2.295 ± 0.335
2.778IleThr: 2.778 ± 0.497
3.805IleVal: 3.805 ± 0.42
0.423IleTrp: 0.423 ± 0.156
1.208IleTyr: 1.208 ± 0.248
0.0IleXaa: 0.0 ± 0.0
Lys
3.926LysAla: 3.926 ± 0.806
0.0LysCys: 0.0 ± 0.0
1.631LysAsp: 1.631 ± 0.283
1.51LysGlu: 1.51 ± 0.346
1.027LysPhe: 1.027 ± 0.227
2.476LysGly: 2.476 ± 0.441
0.544LysHis: 0.544 ± 0.167
2.054LysIle: 2.054 ± 0.322
1.329LysLys: 1.329 ± 0.282
2.778LysLeu: 2.778 ± 0.481
0.302LysMet: 0.302 ± 0.167
1.268LysAsn: 1.268 ± 0.321
2.657LysPro: 2.657 ± 0.412
1.148LysGln: 1.148 ± 0.291
2.295LysArg: 2.295 ± 0.347
1.872LysSer: 1.872 ± 0.322
2.537LysThr: 2.537 ± 0.409
2.416LysVal: 2.416 ± 0.342
0.725LysTrp: 0.725 ± 0.246
0.906LysTyr: 0.906 ± 0.218
0.0LysXaa: 0.0 ± 0.0
Leu
10.268LeuAla: 10.268 ± 1.103
0.664LeuCys: 0.664 ± 0.238
6.281LeuAsp: 6.281 ± 0.735
4.107LeuGlu: 4.107 ± 0.62
2.657LeuPhe: 2.657 ± 0.528
5.617LeuGly: 5.617 ± 0.508
1.389LeuHis: 1.389 ± 0.413
3.745LeuIle: 3.745 ± 0.483
2.476LeuLys: 2.476 ± 0.372
5.315LeuLeu: 5.315 ± 0.594
1.45LeuMet: 1.45 ± 0.281
2.174LeuAsn: 2.174 ± 0.353
4.409LeuPro: 4.409 ± 0.524
2.476LeuGln: 2.476 ± 0.397
5.436LeuArg: 5.436 ± 0.762
4.288LeuSer: 4.288 ± 0.464
5.194LeuThr: 5.194 ± 0.624
4.651LeuVal: 4.651 ± 0.542
1.631LeuTrp: 1.631 ± 0.317
1.148LeuTyr: 1.148 ± 0.297
0.0LeuXaa: 0.0 ± 0.0
Met
2.235MetAla: 2.235 ± 0.469
0.242MetCys: 0.242 ± 0.111
0.664MetAsp: 0.664 ± 0.28
0.785MetGlu: 0.785 ± 0.191
0.121MetPhe: 0.121 ± 0.095
1.872MetGly: 1.872 ± 0.436
0.483MetHis: 0.483 ± 0.145
0.785MetIle: 0.785 ± 0.229
0.906MetLys: 0.906 ± 0.274
1.57MetLeu: 1.57 ± 0.359
0.302MetMet: 0.302 ± 0.15
0.664MetAsn: 0.664 ± 0.258
1.268MetPro: 1.268 ± 0.267
0.725MetGln: 0.725 ± 0.17
2.114MetArg: 2.114 ± 0.364
1.57MetSer: 1.57 ± 0.3
3.02MetThr: 3.02 ± 0.423
1.087MetVal: 1.087 ± 0.208
0.423MetTrp: 0.423 ± 0.193
0.181MetTyr: 0.181 ± 0.104
0.0MetXaa: 0.0 ± 0.0
Asn
3.382AsnAla: 3.382 ± 0.429
0.121AsnCys: 0.121 ± 0.089
2.295AsnAsp: 2.295 ± 0.33
0.966AsnGlu: 0.966 ± 0.27
0.604AsnPhe: 0.604 ± 0.198
3.261AsnGly: 3.261 ± 0.331
1.268AsnHis: 1.268 ± 0.29
1.208AsnIle: 1.208 ± 0.24
0.725AsnLys: 0.725 ± 0.209
1.872AsnLeu: 1.872 ± 0.287
0.423AsnMet: 0.423 ± 0.149
1.027AsnAsn: 1.027 ± 0.239
1.993AsnPro: 1.993 ± 0.416
1.148AsnGln: 1.148 ± 0.253
2.959AsnArg: 2.959 ± 0.357
1.933AsnSer: 1.933 ± 0.295
2.355AsnThr: 2.355 ± 0.58
2.174AsnVal: 2.174 ± 0.38
0.483AsnTrp: 0.483 ± 0.144
0.906AsnTyr: 0.906 ± 0.265
0.0AsnXaa: 0.0 ± 0.0
Pro
7.187ProAla: 7.187 ± 0.591
0.544ProCys: 0.544 ± 0.27
5.013ProAsp: 5.013 ± 0.88
3.865ProGlu: 3.865 ± 0.609
1.389ProPhe: 1.389 ± 0.273
6.221ProGly: 6.221 ± 0.642
0.906ProHis: 0.906 ± 0.299
2.235ProIle: 2.235 ± 0.354
1.933ProLys: 1.933 ± 0.403
3.624ProLeu: 3.624 ± 0.403
1.389ProMet: 1.389 ± 0.251
1.812ProAsn: 1.812 ± 0.301
2.597ProPro: 2.597 ± 0.466
1.51ProGln: 1.51 ± 0.288
4.409ProArg: 4.409 ± 0.768
2.778ProSer: 2.778 ± 0.391
3.926ProThr: 3.926 ± 0.487
3.443ProVal: 3.443 ± 0.448
1.45ProTrp: 1.45 ± 0.263
0.725ProTyr: 0.725 ± 0.214
0.0ProXaa: 0.0 ± 0.0
Gln
3.926GlnAla: 3.926 ± 0.505
0.362GlnCys: 0.362 ± 0.146
1.872GlnAsp: 1.872 ± 0.347
1.208GlnGlu: 1.208 ± 0.295
0.906GlnPhe: 0.906 ± 0.216
2.718GlnGly: 2.718 ± 0.661
0.846GlnHis: 0.846 ± 0.197
1.872GlnIle: 1.872 ± 0.392
1.51GlnLys: 1.51 ± 0.394
4.228GlnLeu: 4.228 ± 0.613
1.329GlnMet: 1.329 ± 0.274
0.966GlnAsn: 0.966 ± 0.238
1.933GlnPro: 1.933 ± 0.273
1.45GlnGln: 1.45 ± 0.355
3.563GlnArg: 3.563 ± 0.526
1.752GlnSer: 1.752 ± 0.338
1.933GlnThr: 1.933 ± 0.406
3.322GlnVal: 3.322 ± 0.552
0.966GlnTrp: 0.966 ± 0.249
0.785GlnTyr: 0.785 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
7.791ArgAla: 7.791 ± 0.693
0.483ArgCys: 0.483 ± 0.227
4.469ArgAsp: 4.469 ± 0.573
4.771ArgGlu: 4.771 ± 0.673
1.993ArgPhe: 1.993 ± 0.294
5.013ArgGly: 5.013 ± 0.601
1.329ArgHis: 1.329 ± 0.353
3.865ArgIle: 3.865 ± 0.591
2.959ArgLys: 2.959 ± 0.437
6.704ArgLeu: 6.704 ± 0.623
2.295ArgMet: 2.295 ± 0.359
2.235ArgAsn: 2.235 ± 0.395
4.59ArgPro: 4.59 ± 0.783
2.718ArgGln: 2.718 ± 0.496
5.919ArgArg: 5.919 ± 0.759
3.563ArgSer: 3.563 ± 0.479
5.617ArgThr: 5.617 ± 0.669
5.375ArgVal: 5.375 ± 0.688
1.329ArgTrp: 1.329 ± 0.312
2.537ArgTyr: 2.537 ± 0.369
0.0ArgXaa: 0.0 ± 0.0
Ser
6.644SerAla: 6.644 ± 0.794
0.242SerCys: 0.242 ± 0.132
3.684SerAsp: 3.684 ± 0.534
2.899SerGlu: 2.899 ± 0.42
1.389SerPhe: 1.389 ± 0.276
5.255SerGly: 5.255 ± 0.649
0.785SerHis: 0.785 ± 0.234
2.114SerIle: 2.114 ± 0.42
2.355SerLys: 2.355 ± 0.388
3.624SerLeu: 3.624 ± 0.493
1.389SerMet: 1.389 ± 0.347
1.752SerAsn: 1.752 ± 0.337
2.839SerPro: 2.839 ± 0.5
2.355SerGln: 2.355 ± 0.381
3.624SerArg: 3.624 ± 0.508
2.597SerSer: 2.597 ± 0.435
3.503SerThr: 3.503 ± 0.48
4.651SerVal: 4.651 ± 0.547
1.208SerTrp: 1.208 ± 0.323
1.208SerTyr: 1.208 ± 0.351
0.0SerXaa: 0.0 ± 0.0
Thr
10.811ThrAla: 10.811 ± 1.339
0.544ThrCys: 0.544 ± 0.173
5.315ThrAsp: 5.315 ± 0.522
2.959ThrGlu: 2.959 ± 0.503
1.691ThrPhe: 1.691 ± 0.312
6.402ThrGly: 6.402 ± 0.816
1.148ThrHis: 1.148 ± 0.245
3.926ThrIle: 3.926 ± 0.544
1.933ThrLys: 1.933 ± 0.378
5.919ThrLeu: 5.919 ± 0.679
1.268ThrMet: 1.268 ± 0.305
1.872ThrAsn: 1.872 ± 0.304
4.651ThrPro: 4.651 ± 0.538
1.812ThrGln: 1.812 ± 0.325
4.469ThrArg: 4.469 ± 0.531
4.953ThrSer: 4.953 ± 0.847
5.436ThrThr: 5.436 ± 0.733
6.281ThrVal: 6.281 ± 0.59
1.148ThrTrp: 1.148 ± 0.337
1.631ThrTyr: 1.631 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
7.127ValAla: 7.127 ± 0.784
0.664ValCys: 0.664 ± 0.237
6.1ValAsp: 6.1 ± 0.793
4.53ValGlu: 4.53 ± 0.49
2.416ValPhe: 2.416 ± 0.381
5.134ValGly: 5.134 ± 0.679
1.208ValHis: 1.208 ± 0.241
3.563ValIle: 3.563 ± 0.409
2.174ValLys: 2.174 ± 0.374
4.711ValLeu: 4.711 ± 0.662
0.785ValMet: 0.785 ± 0.192
2.054ValAsn: 2.054 ± 0.427
5.194ValPro: 5.194 ± 0.45
2.778ValGln: 2.778 ± 0.698
6.161ValArg: 6.161 ± 0.621
3.563ValSer: 3.563 ± 0.548
7.731ValThr: 7.731 ± 0.825
5.194ValVal: 5.194 ± 0.74
0.785ValTrp: 0.785 ± 0.249
1.027ValTyr: 1.027 ± 0.234
0.0ValXaa: 0.0 ± 0.0
Trp
1.631TrpAla: 1.631 ± 0.294
0.0TrpCys: 0.0 ± 0.0
0.785TrpAsp: 0.785 ± 0.262
0.906TrpGlu: 0.906 ± 0.255
0.423TrpPhe: 0.423 ± 0.161
1.148TrpGly: 1.148 ± 0.289
0.483TrpHis: 0.483 ± 0.177
0.483TrpIle: 0.483 ± 0.159
0.483TrpLys: 0.483 ± 0.196
1.993TrpLeu: 1.993 ± 0.361
0.664TrpMet: 0.664 ± 0.252
0.483TrpAsn: 0.483 ± 0.154
1.027TrpPro: 1.027 ± 0.247
1.027TrpGln: 1.027 ± 0.246
2.235TrpArg: 2.235 ± 0.485
1.087TrpSer: 1.087 ± 0.294
1.45TrpThr: 1.45 ± 0.297
1.329TrpVal: 1.329 ± 0.274
0.302TrpTrp: 0.302 ± 0.114
0.664TrpTyr: 0.664 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.657TyrAla: 2.657 ± 0.355
0.242TyrCys: 0.242 ± 0.119
1.148TyrAsp: 1.148 ± 0.263
0.906TyrGlu: 0.906 ± 0.296
0.725TyrPhe: 0.725 ± 0.21
2.114TyrGly: 2.114 ± 0.265
0.544TyrHis: 0.544 ± 0.209
0.544TyrIle: 0.544 ± 0.18
0.604TyrLys: 0.604 ± 0.222
1.57TyrLeu: 1.57 ± 0.34
0.544TyrMet: 0.544 ± 0.165
0.362TyrAsn: 0.362 ± 0.151
1.208TyrPro: 1.208 ± 0.309
0.846TyrGln: 0.846 ± 0.25
1.872TyrArg: 1.872 ± 0.492
1.812TyrSer: 1.812 ± 0.268
1.45TyrThr: 1.45 ± 0.264
1.933TyrVal: 1.933 ± 0.34
0.423TyrTrp: 0.423 ± 0.158
0.423TyrTyr: 0.423 ± 0.194
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (16558 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski