Amino acid dipepetide frequency for Sphingobium phage Lacusarx

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.258AlaAla: 13.258 ± 0.89
0.83AlaCys: 0.83 ± 0.242
6.535AlaAsp: 6.535 ± 0.423
6.696AlaGlu: 6.696 ± 0.447
4.178AlaPhe: 4.178 ± 0.34
8.116AlaGly: 8.116 ± 0.822
1.848AlaHis: 1.848 ± 0.254
5.893AlaIle: 5.893 ± 0.429
5.33AlaLys: 5.33 ± 0.493
7.928AlaLeu: 7.928 ± 0.507
3.669AlaMet: 3.669 ± 0.336
3.669AlaAsn: 3.669 ± 0.363
5.035AlaPro: 5.035 ± 0.46
2.759AlaGln: 2.759 ± 0.355
5.812AlaArg: 5.812 ± 0.343
5.544AlaSer: 5.544 ± 0.338
5.732AlaThr: 5.732 ± 0.446
6.348AlaVal: 6.348 ± 0.441
1.286AlaTrp: 1.286 ± 0.165
3.402AlaTyr: 3.402 ± 0.266
0.0AlaXaa: 0.0 ± 0.0
Cys
1.071CysAla: 1.071 ± 0.191
0.187CysCys: 0.187 ± 0.073
0.696CysAsp: 0.696 ± 0.149
0.67CysGlu: 0.67 ± 0.147
0.375CysPhe: 0.375 ± 0.093
0.964CysGly: 0.964 ± 0.179
0.348CysHis: 0.348 ± 0.094
0.482CysIle: 0.482 ± 0.105
0.402CysLys: 0.402 ± 0.117
0.616CysLeu: 0.616 ± 0.166
0.107CysMet: 0.107 ± 0.048
0.482CysAsn: 0.482 ± 0.131
0.562CysPro: 0.562 ± 0.131
0.375CysGln: 0.375 ± 0.1
0.643CysArg: 0.643 ± 0.131
0.75CysSer: 0.75 ± 0.162
0.482CysThr: 0.482 ± 0.094
0.536CysVal: 0.536 ± 0.127
0.295CysTrp: 0.295 ± 0.092
0.268CysTyr: 0.268 ± 0.078
0.0CysXaa: 0.0 ± 0.0
Asp
6.643AspAla: 6.643 ± 0.398
0.723AspCys: 0.723 ± 0.124
4.741AspAsp: 4.741 ± 0.497
4.687AspGlu: 4.687 ± 0.346
2.893AspPhe: 2.893 ± 0.285
5.893AspGly: 5.893 ± 0.44
1.339AspHis: 1.339 ± 0.19
3.884AspIle: 3.884 ± 0.304
2.705AspLys: 2.705 ± 0.265
5.437AspLeu: 5.437 ± 0.439
1.687AspMet: 1.687 ± 0.207
2.196AspAsn: 2.196 ± 0.218
3.268AspPro: 3.268 ± 0.305
1.821AspGln: 1.821 ± 0.17
4.58AspArg: 4.58 ± 0.424
3.0AspSer: 3.0 ± 0.252
3.723AspThr: 3.723 ± 0.365
3.696AspVal: 3.696 ± 0.313
1.393AspTrp: 1.393 ± 0.184
2.25AspTyr: 2.25 ± 0.285
0.0AspXaa: 0.0 ± 0.0
Glu
8.598GluAla: 8.598 ± 0.622
0.67GluCys: 0.67 ± 0.141
3.911GluAsp: 3.911 ± 0.322
5.116GluGlu: 5.116 ± 0.549
2.411GluPhe: 2.411 ± 0.259
4.205GluGly: 4.205 ± 0.338
1.366GluHis: 1.366 ± 0.221
4.125GluIle: 4.125 ± 0.349
2.839GluLys: 2.839 ± 0.291
6.134GluLeu: 6.134 ± 0.421
1.634GluMet: 1.634 ± 0.217
2.009GluAsn: 2.009 ± 0.251
2.786GluPro: 2.786 ± 0.336
1.687GluGln: 1.687 ± 0.245
4.152GluArg: 4.152 ± 0.383
2.491GluSer: 2.491 ± 0.25
3.348GluThr: 3.348 ± 0.394
4.205GluVal: 4.205 ± 0.322
1.393GluTrp: 1.393 ± 0.185
1.5GluTyr: 1.5 ± 0.204
0.0GluXaa: 0.0 ± 0.0
Phe
3.455PheAla: 3.455 ± 0.356
0.455PheCys: 0.455 ± 0.109
3.536PheAsp: 3.536 ± 0.328
2.277PheGlu: 2.277 ± 0.3
1.527PhePhe: 1.527 ± 0.198
3.696PheGly: 3.696 ± 0.292
1.125PheHis: 1.125 ± 0.201
1.741PheIle: 1.741 ± 0.226
2.277PheLys: 2.277 ± 0.288
2.92PheLeu: 2.92 ± 0.227
0.964PheMet: 0.964 ± 0.169
0.991PheAsn: 0.991 ± 0.14
1.768PhePro: 1.768 ± 0.228
0.857PheGln: 0.857 ± 0.181
2.223PheArg: 2.223 ± 0.192
2.411PheSer: 2.411 ± 0.257
2.598PheThr: 2.598 ± 0.268
2.678PheVal: 2.678 ± 0.244
0.536PheTrp: 0.536 ± 0.131
1.125PheTyr: 1.125 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
7.151GlyAla: 7.151 ± 0.653
0.67GlyCys: 0.67 ± 0.137
4.794GlyAsp: 4.794 ± 0.367
4.741GlyGlu: 4.741 ± 0.328
3.348GlyPhe: 3.348 ± 0.297
7.553GlyGly: 7.553 ± 1.001
1.42GlyHis: 1.42 ± 0.222
4.205GlyIle: 4.205 ± 0.436
4.553GlyLys: 4.553 ± 0.332
5.866GlyLeu: 5.866 ± 0.603
2.384GlyMet: 2.384 ± 0.24
2.33GlyAsn: 2.33 ± 0.314
2.545GlyPro: 2.545 ± 0.345
2.518GlyGln: 2.518 ± 0.251
5.169GlyArg: 5.169 ± 0.382
5.169GlySer: 5.169 ± 0.542
5.196GlyThr: 5.196 ± 0.46
5.089GlyVal: 5.089 ± 0.406
1.607GlyTrp: 1.607 ± 0.227
3.107GlyTyr: 3.107 ± 0.286
0.0GlyXaa: 0.0 ± 0.0
His
1.687HisAla: 1.687 ± 0.21
0.268HisCys: 0.268 ± 0.077
1.58HisAsp: 1.58 ± 0.214
1.5HisGlu: 1.5 ± 0.233
0.991HisPhe: 0.991 ± 0.181
1.821HisGly: 1.821 ± 0.278
0.75HisHis: 0.75 ± 0.16
1.152HisIle: 1.152 ± 0.157
0.964HisLys: 0.964 ± 0.172
1.714HisLeu: 1.714 ± 0.258
0.643HisMet: 0.643 ± 0.155
0.723HisAsn: 0.723 ± 0.146
1.232HisPro: 1.232 ± 0.198
0.429HisGln: 0.429 ± 0.096
1.554HisArg: 1.554 ± 0.211
1.259HisSer: 1.259 ± 0.183
0.964HisThr: 0.964 ± 0.152
1.339HisVal: 1.339 ± 0.156
0.268HisTrp: 0.268 ± 0.089
1.045HisTyr: 1.045 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
5.812IleAla: 5.812 ± 0.434
0.562IleCys: 0.562 ± 0.124
4.607IleAsp: 4.607 ± 0.359
4.714IleGlu: 4.714 ± 0.401
1.393IlePhe: 1.393 ± 0.191
4.178IleGly: 4.178 ± 0.389
1.473IleHis: 1.473 ± 0.248
3.027IleIle: 3.027 ± 0.326
3.08IleLys: 3.08 ± 0.289
3.562IleLeu: 3.562 ± 0.281
1.312IleMet: 1.312 ± 0.196
1.928IleAsn: 1.928 ± 0.26
2.786IlePro: 2.786 ± 0.326
1.366IleGln: 1.366 ± 0.196
3.428IleArg: 3.428 ± 0.29
3.268IleSer: 3.268 ± 0.303
3.884IleThr: 3.884 ± 0.281
3.911IleVal: 3.911 ± 0.357
0.723IleTrp: 0.723 ± 0.137
1.393IleTyr: 1.393 ± 0.215
0.0IleXaa: 0.0 ± 0.0
Lys
5.705LysAla: 5.705 ± 0.547
0.134LysCys: 0.134 ± 0.068
3.268LysAsp: 3.268 ± 0.313
2.812LysGlu: 2.812 ± 0.293
1.982LysPhe: 1.982 ± 0.362
3.696LysGly: 3.696 ± 0.368
0.911LysHis: 0.911 ± 0.182
2.705LysIle: 2.705 ± 0.277
2.866LysLys: 2.866 ± 0.351
4.5LysLeu: 4.5 ± 0.429
1.5LysMet: 1.5 ± 0.228
1.339LysAsn: 1.339 ± 0.186
2.786LysPro: 2.786 ± 0.302
1.527LysGln: 1.527 ± 0.175
3.348LysArg: 3.348 ± 0.329
2.518LysSer: 2.518 ± 0.24
2.866LysThr: 2.866 ± 0.258
3.294LysVal: 3.294 ± 0.284
1.232LysTrp: 1.232 ± 0.161
0.964LysTyr: 0.964 ± 0.171
0.0LysXaa: 0.0 ± 0.0
Leu
8.035LeuAla: 8.035 ± 0.48
0.884LeuCys: 0.884 ± 0.143
4.928LeuAsp: 4.928 ± 0.393
4.152LeuGlu: 4.152 ± 0.295
2.786LeuPhe: 2.786 ± 0.306
5.812LeuGly: 5.812 ± 0.521
1.848LeuHis: 1.848 ± 0.257
4.232LeuIle: 4.232 ± 0.342
3.428LeuLys: 3.428 ± 0.341
5.625LeuLeu: 5.625 ± 0.448
2.196LeuMet: 2.196 ± 0.239
2.839LeuAsn: 2.839 ± 0.26
4.741LeuPro: 4.741 ± 0.388
3.0LeuGln: 3.0 ± 0.327
4.982LeuArg: 4.982 ± 0.4
5.571LeuSer: 5.571 ± 0.398
5.062LeuThr: 5.062 ± 0.341
5.062LeuVal: 5.062 ± 0.316
1.045LeuTrp: 1.045 ± 0.167
2.223LeuTyr: 2.223 ± 0.226
0.0LeuXaa: 0.0 ± 0.0
Met
2.893MetAla: 2.893 ± 0.274
0.214MetCys: 0.214 ± 0.078
1.768MetAsp: 1.768 ± 0.229
1.554MetGlu: 1.554 ± 0.203
1.098MetPhe: 1.098 ± 0.169
1.928MetGly: 1.928 ± 0.278
0.482MetHis: 0.482 ± 0.091
1.661MetIle: 1.661 ± 0.217
1.554MetLys: 1.554 ± 0.237
1.928MetLeu: 1.928 ± 0.2
1.125MetMet: 1.125 ± 0.221
1.259MetAsn: 1.259 ± 0.164
1.205MetPro: 1.205 ± 0.152
1.071MetGln: 1.071 ± 0.182
1.661MetArg: 1.661 ± 0.224
2.036MetSer: 2.036 ± 0.254
2.357MetThr: 2.357 ± 0.273
1.741MetVal: 1.741 ± 0.283
0.402MetTrp: 0.402 ± 0.102
0.268MetTyr: 0.268 ± 0.086
0.0MetXaa: 0.0 ± 0.0
Asn
3.75AsnAla: 3.75 ± 0.32
0.402AsnCys: 0.402 ± 0.111
2.062AsnAsp: 2.062 ± 0.235
2.062AsnGlu: 2.062 ± 0.241
1.098AsnPhe: 1.098 ± 0.175
2.946AsnGly: 2.946 ± 0.266
0.964AsnHis: 0.964 ± 0.156
1.821AsnIle: 1.821 ± 0.259
1.768AsnLys: 1.768 ± 0.202
3.268AsnLeu: 3.268 ± 0.346
0.589AsnMet: 0.589 ± 0.13
0.777AsnAsn: 0.777 ± 0.159
2.062AsnPro: 2.062 ± 0.236
0.911AsnGln: 0.911 ± 0.15
1.848AsnArg: 1.848 ± 0.181
1.795AsnSer: 1.795 ± 0.194
1.875AsnThr: 1.875 ± 0.211
1.848AsnVal: 1.848 ± 0.224
0.455AsnTrp: 0.455 ± 0.094
1.339AsnTyr: 1.339 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
5.009ProAla: 5.009 ± 0.446
0.643ProCys: 0.643 ± 0.125
3.616ProAsp: 3.616 ± 0.351
3.402ProGlu: 3.402 ± 0.314
1.42ProPhe: 1.42 ± 0.189
3.616ProGly: 3.616 ± 0.347
1.205ProHis: 1.205 ± 0.161
2.973ProIle: 2.973 ± 0.284
2.732ProLys: 2.732 ± 0.249
3.562ProLeu: 3.562 ± 0.291
1.232ProMet: 1.232 ± 0.16
1.607ProAsn: 1.607 ± 0.22
2.491ProPro: 2.491 ± 0.354
1.098ProGln: 1.098 ± 0.129
2.303ProArg: 2.303 ± 0.283
2.92ProSer: 2.92 ± 0.236
2.839ProThr: 2.839 ± 0.28
3.08ProVal: 3.08 ± 0.295
0.536ProTrp: 0.536 ± 0.155
1.5ProTyr: 1.5 ± 0.195
0.0ProXaa: 0.0 ± 0.0
Gln
3.053GlnAla: 3.053 ± 0.271
0.321GlnCys: 0.321 ± 0.09
1.339GlnAsp: 1.339 ± 0.178
1.714GlnGlu: 1.714 ± 0.273
1.179GlnPhe: 1.179 ± 0.134
1.982GlnGly: 1.982 ± 0.238
0.777GlnHis: 0.777 ± 0.142
1.875GlnIle: 1.875 ± 0.252
1.366GlnLys: 1.366 ± 0.221
2.303GlnLeu: 2.303 ± 0.227
1.045GlnMet: 1.045 ± 0.204
0.991GlnAsn: 0.991 ± 0.159
0.911GlnPro: 0.911 ± 0.155
1.339GlnGln: 1.339 ± 0.258
2.491GlnArg: 2.491 ± 0.229
1.607GlnSer: 1.607 ± 0.183
1.902GlnThr: 1.902 ± 0.251
1.928GlnVal: 1.928 ± 0.207
0.67GlnTrp: 0.67 ± 0.159
0.937GlnTyr: 0.937 ± 0.136
0.0GlnXaa: 0.0 ± 0.0
Arg
5.625ArgAla: 5.625 ± 0.323
0.991ArgCys: 0.991 ± 0.177
4.366ArgAsp: 4.366 ± 0.296
3.643ArgGlu: 3.643 ± 0.394
2.786ArgPhe: 2.786 ± 0.248
3.589ArgGly: 3.589 ± 0.268
1.554ArgHis: 1.554 ± 0.199
3.321ArgIle: 3.321 ± 0.271
2.866ArgLys: 2.866 ± 0.273
4.821ArgLeu: 4.821 ± 0.361
1.928ArgMet: 1.928 ± 0.265
2.357ArgAsn: 2.357 ± 0.263
2.464ArgPro: 2.464 ± 0.251
1.955ArgGln: 1.955 ± 0.226
4.928ArgArg: 4.928 ± 0.48
3.053ArgSer: 3.053 ± 0.327
3.161ArgThr: 3.161 ± 0.331
3.911ArgVal: 3.911 ± 0.398
1.205ArgTrp: 1.205 ± 0.181
2.277ArgTyr: 2.277 ± 0.228
0.0ArgXaa: 0.0 ± 0.0
Ser
5.571SerAla: 5.571 ± 0.625
0.402SerCys: 0.402 ± 0.115
3.75SerAsp: 3.75 ± 0.291
3.348SerGlu: 3.348 ± 0.384
2.92SerPhe: 2.92 ± 0.327
5.089SerGly: 5.089 ± 0.407
0.964SerHis: 0.964 ± 0.142
3.294SerIle: 3.294 ± 0.35
2.732SerLys: 2.732 ± 0.279
4.821SerLeu: 4.821 ± 0.35
1.795SerMet: 1.795 ± 0.21
2.17SerAsn: 2.17 ± 0.333
2.625SerPro: 2.625 ± 0.222
1.58SerGln: 1.58 ± 0.212
2.973SerArg: 2.973 ± 0.262
3.803SerSer: 3.803 ± 0.402
2.973SerThr: 2.973 ± 0.295
3.321SerVal: 3.321 ± 0.296
1.125SerTrp: 1.125 ± 0.19
1.955SerTyr: 1.955 ± 0.187
0.0SerXaa: 0.0 ± 0.0
Thr
6.241ThrAla: 6.241 ± 0.52
0.482ThrCys: 0.482 ± 0.112
3.75ThrAsp: 3.75 ± 0.311
3.402ThrGlu: 3.402 ± 0.312
2.17ThrPhe: 2.17 ± 0.263
5.785ThrGly: 5.785 ± 0.468
1.366ThrHis: 1.366 ± 0.218
3.937ThrIle: 3.937 ± 0.357
3.161ThrLys: 3.161 ± 0.339
4.5ThrLeu: 4.5 ± 0.349
1.527ThrMet: 1.527 ± 0.184
2.116ThrAsn: 2.116 ± 0.21
3.643ThrPro: 3.643 ± 0.331
1.902ThrGln: 1.902 ± 0.26
2.705ThrArg: 2.705 ± 0.269
3.268ThrSer: 3.268 ± 0.333
4.071ThrThr: 4.071 ± 0.483
4.071ThrVal: 4.071 ± 0.367
0.509ThrTrp: 0.509 ± 0.125
2.009ThrTyr: 2.009 ± 0.304
0.0ThrXaa: 0.0 ± 0.0
Val
6.455ValAla: 6.455 ± 0.536
0.643ValCys: 0.643 ± 0.142
3.723ValAsp: 3.723 ± 0.348
5.143ValGlu: 5.143 ± 0.421
2.839ValPhe: 2.839 ± 0.304
4.821ValGly: 4.821 ± 0.371
1.045ValHis: 1.045 ± 0.191
3.803ValIle: 3.803 ± 0.317
3.294ValLys: 3.294 ± 0.288
4.205ValLeu: 4.205 ± 0.394
1.661ValMet: 1.661 ± 0.258
2.062ValAsn: 2.062 ± 0.202
3.0ValPro: 3.0 ± 0.291
1.955ValGln: 1.955 ± 0.223
3.241ValArg: 3.241 ± 0.324
3.964ValSer: 3.964 ± 0.366
4.607ValThr: 4.607 ± 0.406
5.143ValVal: 5.143 ± 0.438
1.071ValTrp: 1.071 ± 0.189
1.58ValTyr: 1.58 ± 0.22
0.0ValXaa: 0.0 ± 0.0
Trp
1.259TrpAla: 1.259 ± 0.16
0.268TrpCys: 0.268 ± 0.088
0.964TrpAsp: 0.964 ± 0.16
0.937TrpGlu: 0.937 ± 0.152
0.589TrpPhe: 0.589 ± 0.137
1.446TrpGly: 1.446 ± 0.235
0.455TrpHis: 0.455 ± 0.11
0.857TrpIle: 0.857 ± 0.161
0.804TrpLys: 0.804 ± 0.125
1.527TrpLeu: 1.527 ± 0.209
0.482TrpMet: 0.482 ± 0.084
0.777TrpAsn: 0.777 ± 0.14
0.696TrpPro: 0.696 ± 0.138
0.562TrpGln: 0.562 ± 0.117
1.232TrpArg: 1.232 ± 0.205
1.045TrpSer: 1.045 ± 0.167
1.125TrpThr: 1.125 ± 0.23
0.911TrpVal: 0.911 ± 0.199
0.295TrpTrp: 0.295 ± 0.113
0.375TrpTyr: 0.375 ± 0.1
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.625TyrAla: 2.625 ± 0.27
0.562TyrCys: 0.562 ± 0.123
2.625TyrAsp: 2.625 ± 0.294
2.009TyrGlu: 2.009 ± 0.197
1.179TyrPhe: 1.179 ± 0.195
2.277TyrGly: 2.277 ± 0.276
0.643TyrHis: 0.643 ± 0.126
1.42TyrIle: 1.42 ± 0.198
1.232TyrLys: 1.232 ± 0.202
3.214TyrLeu: 3.214 ± 0.257
0.696TyrMet: 0.696 ± 0.137
1.018TyrAsn: 1.018 ± 0.172
1.179TyrPro: 1.179 ± 0.185
1.018TyrGln: 1.018 ± 0.171
1.527TyrArg: 1.527 ± 0.199
1.741TyrSer: 1.741 ± 0.199
1.848TyrThr: 1.848 ± 0.254
2.143TyrVal: 2.143 ± 0.226
0.562TyrTrp: 0.562 ± 0.152
1.045TyrTyr: 1.045 ± 0.172
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 196 proteins (37336 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski