Amino acid dipepetide frequency for Pelagibaculum spongiae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.434AlaAla: 8.434 ± 0.113	1.061AlaCys: 1.061 ± 0.03	5.072AlaAsp: 5.072 ± 0.083	5.896AlaGlu: 5.896 ± 0.078	3.254AlaPhe: 3.254 ± 0.066	6.305AlaGly: 6.305 ± 0.08	1.471AlaHis: 1.471 ± 0.036	5.686AlaIle: 5.686 ± 0.071	4.467AlaLys: 4.467 ± 0.063	9.119AlaLeu: 9.119 ± 0.089	2.472AlaMet: 2.472 ± 0.051	3.424AlaAsn: 3.424 ± 0.062	2.97AlaPro: 2.97 ± 0.061	3.645AlaGln: 3.645 ± 0.057	4.02AlaArg: 4.02 ± 0.066	5.897AlaSer: 5.897 ± 0.075	4.199AlaThr: 4.199 ± 0.06	5.611AlaVal: 5.611 ± 0.076	0.994AlaTrp: 0.994 ± 0.033	2.038AlaTyr: 2.038 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	0.793CysAla: 0.793 ± 0.03	0.211CysCys: 0.211 ± 0.013	0.685CysAsp: 0.685 ± 0.024	0.573CysGlu: 0.573 ± 0.021	0.564CysPhe: 0.564 ± 0.021	1.046CysGly: 1.046 ± 0.031	0.381CysHis: 0.381 ± 0.018	0.635CysIle: 0.635 ± 0.02	0.587CysLys: 0.587 ± 0.019	1.121CysLeu: 1.121 ± 0.032	0.275CysMet: 0.275 ± 0.016	0.483CysAsn: 0.483 ± 0.019	0.512CysPro: 0.512 ± 0.022	0.657CysGln: 0.657 ± 0.027	0.609CysArg: 0.609 ± 0.022	0.89CysSer: 0.89 ± 0.025	0.486CysThr: 0.486 ± 0.019	0.553CysVal: 0.553 ± 0.023	0.201CysTrp: 0.201 ± 0.012	0.387CysTyr: 0.387 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.297AspAla: 4.297 ± 0.069	0.667AspCys: 0.667 ± 0.021	3.065AspAsp: 3.065 ± 0.064	3.159AspGlu: 3.159 ± 0.055	2.733AspPhe: 2.733 ± 0.048	3.979AspGly: 3.979 ± 0.097	1.24AspHis: 1.24 ± 0.031	3.592AspIle: 3.592 ± 0.061	2.825AspLys: 2.825 ± 0.052	6.035AspLeu: 6.035 ± 0.077	1.207AspMet: 1.207 ± 0.031	2.358AspAsn: 2.358 ± 0.077	2.494AspPro: 2.494 ± 0.048	3.517AspGln: 3.517 ± 0.062	2.788AspArg: 2.788 ± 0.048	3.728AspSer: 3.728 ± 0.078	2.386AspThr: 2.386 ± 0.047	3.123AspVal: 3.123 ± 0.056	0.894AspTrp: 0.894 ± 0.028	1.961AspTyr: 1.961 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	4.775GluAla: 4.775 ± 0.081	0.502GluCys: 0.502 ± 0.023	2.807GluAsp: 2.807 ± 0.048	2.806GluGlu: 2.806 ± 0.061	2.18GluPhe: 2.18 ± 0.046	3.097GluGly: 3.097 ± 0.058	1.274GluHis: 1.274 ± 0.035	4.12GluIle: 4.12 ± 0.062	4.019GluLys: 4.019 ± 0.058	6.57GluLeu: 6.57 ± 0.069	1.689GluMet: 1.689 ± 0.039	2.874GluAsn: 2.874 ± 0.048	1.929GluPro: 1.929 ± 0.04	4.174GluGln: 4.174 ± 0.08	2.784GluArg: 2.784 ± 0.048	3.666GluSer: 3.666 ± 0.049	3.04GluThr: 3.04 ± 0.055	3.769GluVal: 3.769 ± 0.061	0.645GluTrp: 0.645 ± 0.021	1.698GluTyr: 1.698 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	3.293PheAla: 3.293 ± 0.05	0.611PheCys: 0.611 ± 0.022	2.672PheAsp: 2.672 ± 0.047	2.422PheGlu: 2.422 ± 0.048	1.867PhePhe: 1.867 ± 0.05	2.878PheGly: 2.878 ± 0.053	0.847PheHis: 0.847 ± 0.025	2.481PheIle: 2.481 ± 0.048	2.042PheLys: 2.042 ± 0.038	3.56PheLeu: 3.56 ± 0.054	0.916PheMet: 0.916 ± 0.027	1.952PheAsn: 1.952 ± 0.042	1.576PhePro: 1.576 ± 0.046	1.578PheGln: 1.578 ± 0.036	1.752PheArg: 1.752 ± 0.044	3.862PheSer: 3.862 ± 0.057	2.108PheThr: 2.108 ± 0.044	2.325PheVal: 2.325 ± 0.044	0.561PheTrp: 0.561 ± 0.018	1.37PheTyr: 1.37 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.891GlyAla: 4.891 ± 0.068	0.991GlyCys: 0.991 ± 0.027	3.728GlyAsp: 3.728 ± 0.076	4.1GlyGlu: 4.1 ± 0.062	3.282GlyPhe: 3.282 ± 0.05	4.799GlyGly: 4.799 ± 0.075	1.497GlyHis: 1.497 ± 0.036	4.338GlyIle: 4.338 ± 0.062	4.012GlyLys: 4.012 ± 0.053	7.111GlyLeu: 7.111 ± 0.106	1.918GlyMet: 1.918 ± 0.044	2.647GlyAsn: 2.647 ± 0.05	1.676GlyPro: 1.676 ± 0.04	3.229GlyGln: 3.229 ± 0.058	3.326GlyArg: 3.326 ± 0.06	4.381GlySer: 4.381 ± 0.064	2.9GlyThr: 2.9 ± 0.054	4.595GlyVal: 4.595 ± 0.073	1.132GlyTrp: 1.132 ± 0.034	2.386GlyTyr: 2.386 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.532HisAla: 1.532 ± 0.037	0.378HisCys: 0.378 ± 0.016	0.961HisAsp: 0.961 ± 0.026	0.927HisGlu: 0.927 ± 0.026	1.019HisPhe: 1.019 ± 0.029	1.447HisGly: 1.447 ± 0.035	0.645HisHis: 0.645 ± 0.027	1.164HisIle: 1.164 ± 0.032	1.005HisLys: 1.005 ± 0.029	2.33HisLeu: 2.33 ± 0.045	0.489HisMet: 0.489 ± 0.017	0.778HisAsn: 0.778 ± 0.025	1.182HisPro: 1.182 ± 0.029	1.409HisGln: 1.409 ± 0.037	1.11HisArg: 1.11 ± 0.026	1.514HisSer: 1.514 ± 0.034	0.868HisThr: 0.868 ± 0.025	1.069HisVal: 1.069 ± 0.027	0.398HisTrp: 0.398 ± 0.018	0.768HisTyr: 0.768 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.027IleAla: 6.027 ± 0.067	0.799IleCys: 0.799 ± 0.027	4.371IleAsp: 4.371 ± 0.062	4.691IleGlu: 4.691 ± 0.064	2.335IlePhe: 2.335 ± 0.044	4.354IleGly: 4.354 ± 0.061	1.246IleHis: 1.246 ± 0.032	3.54IleIle: 3.54 ± 0.057	3.397IleLys: 3.397 ± 0.058	5.116IleLeu: 5.116 ± 0.077	1.169IleMet: 1.169 ± 0.03	3.183IleAsn: 3.183 ± 0.054	2.653IlePro: 2.653 ± 0.045	2.562IleGln: 2.562 ± 0.043	3.035IleArg: 3.035 ± 0.05	5.296IleSer: 5.296 ± 0.068	3.517IleThr: 3.517 ± 0.049	3.361IleVal: 3.361 ± 0.055	0.693IleTrp: 0.693 ± 0.022	1.723IleTyr: 1.723 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.553LysAla: 4.553 ± 0.07	0.394LysCys: 0.394 ± 0.017	2.696LysAsp: 2.696 ± 0.051	2.805LysGlu: 2.805 ± 0.058	1.692LysPhe: 1.692 ± 0.037	3.111LysGly: 3.111 ± 0.052	1.142LysHis: 1.142 ± 0.031	3.771LysIle: 3.771 ± 0.054	3.834LysLys: 3.834 ± 0.067	5.732LysLeu: 5.732 ± 0.071	1.408LysMet: 1.408 ± 0.037	2.712LysAsn: 2.712 ± 0.05	2.552LysPro: 2.552 ± 0.044	3.277LysGln: 3.277 ± 0.057	2.59LysArg: 2.59 ± 0.042	3.539LysSer: 3.539 ± 0.061	2.884LysThr: 2.884 ± 0.044	3.278LysVal: 3.278 ± 0.047	0.543LysTrp: 0.543 ± 0.02	1.355LysTyr: 1.355 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	10.195LeuAla: 10.195 ± 0.099	1.146LeuCys: 1.146 ± 0.031	6.213LeuAsp: 6.213 ± 0.078	6.487LeuGlu: 6.487 ± 0.085	4.117LeuPhe: 4.117 ± 0.073	6.537LeuGly: 6.537 ± 0.088	1.991LeuHis: 1.991 ± 0.038	6.711LeuIle: 6.711 ± 0.092	6.025LeuLys: 6.025 ± 0.073	12.13LeuLeu: 12.13 ± 0.14	2.663LeuMet: 2.663 ± 0.048	4.578LeuAsn: 4.578 ± 0.076	5.317LeuPro: 5.317 ± 0.079	5.417LeuGln: 5.417 ± 0.097	4.509LeuArg: 4.509 ± 0.064	7.904LeuSer: 7.904 ± 0.083	5.486LeuThr: 5.486 ± 0.07	6.934LeuVal: 6.934 ± 0.084	1.213LeuTrp: 1.213 ± 0.032	2.444LeuTyr: 2.444 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.491MetAla: 2.491 ± 0.05	0.182MetCys: 0.182 ± 0.011	1.31MetAsp: 1.31 ± 0.031	1.265MetGlu: 1.265 ± 0.03	0.736MetPhe: 0.736 ± 0.024	1.6MetGly: 1.6 ± 0.039	0.441MetHis: 0.441 ± 0.017	1.497MetIle: 1.497 ± 0.033	1.379MetLys: 1.379 ± 0.032	2.855MetLeu: 2.855 ± 0.055	0.697MetMet: 0.697 ± 0.026	0.968MetAsn: 0.968 ± 0.025	1.323MetPro: 1.323 ± 0.033	1.248MetGln: 1.248 ± 0.028	1.181MetArg: 1.181 ± 0.029	1.795MetSer: 1.795 ± 0.04	1.316MetThr: 1.316 ± 0.032	1.733MetVal: 1.733 ± 0.044	0.176MetTrp: 0.176 ± 0.011	0.42MetTyr: 0.42 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.346AsnAla: 3.346 ± 0.061	0.556AsnCys: 0.556 ± 0.019	2.281AsnAsp: 2.281 ± 0.071	2.004AsnGlu: 2.004 ± 0.04	1.733AsnPhe: 1.733 ± 0.039	3.047AsnGly: 3.047 ± 0.062	1.035AsnHis: 1.035 ± 0.03	2.781AsnIle: 2.781 ± 0.048	2.309AsnLys: 2.309 ± 0.048	4.144AsnLeu: 4.144 ± 0.066	0.93AsnMet: 0.93 ± 0.024	2.266AsnAsn: 2.266 ± 0.061	2.309AsnPro: 2.309 ± 0.045	2.882AsnGln: 2.882 ± 0.062	2.223AsnArg: 2.223 ± 0.043	3.035AsnSer: 3.035 ± 0.067	2.071AsnThr: 2.071 ± 0.045	1.982AsnVal: 1.982 ± 0.038	0.699AsnTrp: 0.699 ± 0.022	1.358AsnTyr: 1.358 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	3.778ProAla: 3.778 ± 0.061	0.368ProCys: 0.368 ± 0.017	2.61ProAsp: 2.61 ± 0.048	3.377ProGlu: 3.377 ± 0.055	1.694ProPhe: 1.694 ± 0.033	2.817ProGly: 2.817 ± 0.046	0.751ProHis: 0.751 ± 0.022	2.443ProIle: 2.443 ± 0.048	2.04ProLys: 2.04 ± 0.044	4.565ProLeu: 4.565 ± 0.069	0.97ProMet: 0.97 ± 0.029	1.514ProAsn: 1.514 ± 0.043	1.325ProPro: 1.325 ± 0.036	2.045ProGln: 2.045 ± 0.051	1.517ProArg: 1.517 ± 0.033	2.671ProSer: 2.671 ± 0.046	2.004ProThr: 2.004 ± 0.039	3.234ProVal: 3.234 ± 0.059	0.616ProTrp: 0.616 ± 0.022	1.071ProTyr: 1.071 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	5.005GlnAla: 5.005 ± 0.074	0.445GlnCys: 0.445 ± 0.021	2.122GlnAsp: 2.122 ± 0.038	2.104GlnGlu: 2.104 ± 0.045	1.912GlnPhe: 1.912 ± 0.038	3.184GlnGly: 3.184 ± 0.051	1.31GlnHis: 1.31 ± 0.034	3.123GlnIle: 3.123 ± 0.056	2.833GlnLys: 2.833 ± 0.05	7.493GlnLeu: 7.493 ± 0.164	1.28GlnMet: 1.28 ± 0.033	1.951GlnAsn: 1.951 ± 0.052	2.689GlnPro: 2.689 ± 0.057	5.47GlnGln: 5.47 ± 0.174	2.707GlnArg: 2.707 ± 0.059	3.412GlnSer: 3.412 ± 0.061	2.445GlnThr: 2.445 ± 0.052	3.368GlnVal: 3.368 ± 0.062	0.855GlnTrp: 0.855 ± 0.027	1.392GlnTyr: 1.392 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	3.466ArgAla: 3.466 ± 0.045	0.548ArgCys: 0.548 ± 0.02	2.423ArgAsp: 2.423 ± 0.045	2.714ArgGlu: 2.714 ± 0.056	2.229ArgPhe: 2.229 ± 0.045	2.645ArgGly: 2.645 ± 0.051	1.178ArgHis: 1.178 ± 0.029	3.131ArgIle: 3.131 ± 0.048	2.693ArgLys: 2.693 ± 0.05	5.655ArgLeu: 5.655 ± 0.069	1.263ArgMet: 1.263 ± 0.035	2.028ArgAsn: 2.028 ± 0.043	1.787ArgPro: 1.787 ± 0.042	2.91ArgGln: 2.91 ± 0.046	2.586ArgArg: 2.586 ± 0.056	3.031ArgSer: 3.031 ± 0.048	1.939ArgThr: 1.939 ± 0.039	3.038ArgVal: 3.038 ± 0.05	0.779ArgTrp: 0.779 ± 0.024	1.687ArgTyr: 1.687 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	5.931SerAla: 5.931 ± 0.066	0.871SerCys: 0.871 ± 0.03	4.111SerAsp: 4.111 ± 0.068	4.276SerGlu: 4.276 ± 0.063	2.965SerPhe: 2.965 ± 0.057	5.55SerGly: 5.55 ± 0.069	1.438SerHis: 1.438 ± 0.03	4.488SerIle: 4.488 ± 0.063	3.422SerLys: 3.422 ± 0.053	7.588SerLeu: 7.588 ± 0.087	1.651SerMet: 1.651 ± 0.04	3.108SerAsn: 3.108 ± 0.06	2.52SerPro: 2.52 ± 0.047	3.68SerGln: 3.68 ± 0.066	3.353SerArg: 3.353 ± 0.058	5.196SerSer: 5.196 ± 0.079	2.988SerThr: 2.988 ± 0.051	4.33SerVal: 4.33 ± 0.057	1.186SerTrp: 1.186 ± 0.03	2.088SerTyr: 2.088 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.481ThrAla: 4.481 ± 0.067	0.534ThrCys: 0.534 ± 0.021	2.98ThrAsp: 2.98 ± 0.064	3.01ThrGlu: 3.01 ± 0.046	1.723ThrPhe: 1.723 ± 0.042	3.99ThrGly: 3.99 ± 0.067	0.951ThrHis: 0.951 ± 0.026	3.069ThrIle: 3.069 ± 0.05	1.859ThrLys: 1.859 ± 0.039	5.221ThrLeu: 5.221 ± 0.068	0.989ThrMet: 0.989 ± 0.025	1.953ThrAsn: 1.953 ± 0.045	2.589ThrPro: 2.589 ± 0.044	1.92ThrGln: 1.92 ± 0.043	2.241ThrArg: 2.241 ± 0.043	3.46ThrSer: 3.46 ± 0.052	2.626ThrThr: 2.626 ± 0.052	3.059ThrVal: 3.059 ± 0.048	0.458ThrTrp: 0.458 ± 0.019	1.098ThrTyr: 1.098 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	5.966ValAla: 5.966 ± 0.079	0.768ValCys: 0.768 ± 0.025	3.956ValAsp: 3.956 ± 0.064	4.096ValGlu: 4.096 ± 0.073	2.636ValPhe: 2.636 ± 0.049	3.923ValGly: 3.923 ± 0.063	1.136ValHis: 1.136 ± 0.028	4.221ValIle: 4.221 ± 0.057	3.132ValLys: 3.132 ± 0.055	6.127ValLeu: 6.127 ± 0.075	1.71ValMet: 1.71 ± 0.04	2.695ValAsn: 2.695 ± 0.047	2.199ValPro: 2.199 ± 0.045	2.147ValGln: 2.147 ± 0.042	2.873ValArg: 2.873 ± 0.05	4.483ValSer: 4.483 ± 0.063	3.312ValThr: 3.312 ± 0.057	4.451ValVal: 4.451 ± 0.069	0.716ValTrp: 0.716 ± 0.024	1.509ValTyr: 1.509 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	0.787TrpAla: 0.787 ± 0.026	0.163TrpCys: 0.163 ± 0.01	0.567TrpAsp: 0.567 ± 0.02	0.502TrpGlu: 0.502 ± 0.018	0.655TrpPhe: 0.655 ± 0.023	0.732TrpGly: 0.732 ± 0.022	0.323TrpHis: 0.323 ± 0.017	0.783TrpIle: 0.783 ± 0.026	0.721TrpLys: 0.721 ± 0.023	2.173TrpLeu: 2.173 ± 0.042	0.35TrpMet: 0.35 ± 0.016	0.529TrpAsn: 0.529 ± 0.019	0.571TrpPro: 0.571 ± 0.021	1.153TrpGln: 1.153 ± 0.033	0.713TrpArg: 0.713 ± 0.023	0.833TrpSer: 0.833 ± 0.029	0.474TrpThr: 0.474 ± 0.019	0.801TrpVal: 0.801 ± 0.028	0.205TrpTrp: 0.205 ± 0.012	0.369TrpTyr: 0.369 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.994TyrAla: 1.994 ± 0.041	0.452TyrCys: 0.452 ± 0.019	1.421TyrAsp: 1.421 ± 0.035	1.206TyrGlu: 1.206 ± 0.029	1.35TyrPhe: 1.35 ± 0.032	1.896TyrGly: 1.896 ± 0.042	0.696TyrHis: 0.696 ± 0.025	1.493TyrIle: 1.493 ± 0.035	1.268TyrLys: 1.268 ± 0.029	3.371TyrLeu: 3.371 ± 0.049	0.567TyrMet: 0.567 ± 0.02	0.99TyrAsn: 0.99 ± 0.031	1.237TyrPro: 1.237 ± 0.029	2.178TyrGln: 2.178 ± 0.047	1.812TyrArg: 1.812 ± 0.039	2.146TyrSer: 2.146 ± 0.039	1.161TyrThr: 1.161 ± 0.034	1.443TyrVal: 1.443 ± 0.033	0.441TyrTrp: 0.441 ± 0.019	0.918TyrTyr: 0.918 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4189 proteins (1371512 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---