Yersinia phage phiR2-01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Haartmanvirus; Yersinia virus phiR201

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7LEF6|I7LEF6_9CAUD D3 protein OS=Yersinia phage phiR2-01 OX=1206557 GN=g104 PE=4 SV=1
MM1 pKa = 7.13EE2 pKa = 5.11RR3 pKa = 11.84KK4 pKa = 8.99YY5 pKa = 9.97WRR7 pKa = 11.84QVDD10 pKa = 3.85PEE12 pKa = 4.35LVEE15 pKa = 4.04HH16 pKa = 7.1AEE18 pKa = 4.06VLLAQWLEE26 pKa = 4.06VPLLNFTFPFTGIADD41 pKa = 3.91EE42 pKa = 4.62CNGVYY47 pKa = 9.89IYY49 pKa = 10.73GQEE52 pKa = 4.29EE53 pKa = 4.18EE54 pKa = 5.81DD55 pKa = 4.48DD56 pKa = 5.39DD57 pKa = 4.91PDD59 pKa = 4.33EE60 pKa = 5.01VPEE63 pKa = 5.12DD64 pKa = 5.0DD65 pKa = 5.3PGCDD69 pKa = 4.14LDD71 pKa = 3.98WNQLSAYY78 pKa = 8.99GLLVALNYY86 pKa = 10.36RR87 pKa = 11.84VFHH90 pKa = 6.02PVGLAIARR98 pKa = 11.84DD99 pKa = 3.86VGTGSSPYY107 pKa = 10.78LLVAEE112 pKa = 4.7DD113 pKa = 5.35GIWEE117 pKa = 4.07YY118 pKa = 11.17SPEE121 pKa = 4.2VIEE124 pKa = 4.58EE125 pKa = 4.64AIAKK129 pKa = 8.97LKK131 pKa = 10.83SFEE134 pKa = 4.3LVVPFLTDD142 pKa = 3.49RR143 pKa = 11.84EE144 pKa = 4.37DD145 pKa = 3.11

Molecular weight: 16.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7LEE8|I7LEE8_9CAUD Putative dihydrofolate reductase OS=Yersinia phage phiR2-01 OX=1206557 GN=g084 PE=4 SV=1
MM1 pKa = 7.77AKK3 pKa = 10.18NKK5 pKa = 9.08NAKK8 pKa = 9.36AQATPAVKK16 pKa = 9.62TFPKK20 pKa = 10.24TEE22 pKa = 4.13ANRR25 pKa = 11.84KK26 pKa = 8.94ARR28 pKa = 11.84LEE30 pKa = 3.84RR31 pKa = 11.84HH32 pKa = 5.4LRR34 pKa = 11.84KK35 pKa = 10.0HH36 pKa = 5.63PTDD39 pKa = 3.67AQAKK43 pKa = 8.05SAVGAPAPIRR53 pKa = 11.84HH54 pKa = 5.98KK55 pKa = 10.61PKK57 pKa = 10.58AKK59 pKa = 10.34NATTSVAKK67 pKa = 10.01EE68 pKa = 3.76VIFIKK73 pKa = 11.0GEE75 pKa = 3.93GHH77 pKa = 6.19KK78 pKa = 10.42SVPVRR83 pKa = 11.84LGFAAGAEE91 pKa = 4.08LFARR95 pKa = 11.84NGMAPKK101 pKa = 9.59EE102 pKa = 4.02YY103 pKa = 10.41EE104 pKa = 3.91KK105 pKa = 11.04AVSQKK110 pKa = 10.32RR111 pKa = 11.84KK112 pKa = 7.72PTAEE116 pKa = 3.97VLRR119 pKa = 11.84AARR122 pKa = 11.84NQFGSVKK129 pKa = 10.08PNIFGVDD136 pKa = 3.24YY137 pKa = 11.34SRR139 pKa = 11.84DD140 pKa = 3.48NVRR143 pKa = 11.84ALCYY147 pKa = 10.32GLNIKK152 pKa = 8.48FTGASDD158 pKa = 3.82RR159 pKa = 11.84KK160 pKa = 8.88ATRR163 pKa = 11.84KK164 pKa = 9.09PVRR167 pKa = 11.84KK168 pKa = 9.88AKK170 pKa = 10.48

Molecular weight: 18.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

154

0

154

30892

41

1226

200.6

22.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.979 ± 0.46

1.046 ± 0.091

6.325 ± 0.187

6.953 ± 0.241

4.072 ± 0.169

6.513 ± 0.192

1.758 ± 0.12

6.516 ± 0.19

6.956 ± 0.232

8.177 ± 0.184

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.134

5.105 ± 0.144

3.525 ± 0.153

3.574 ± 0.189

4.399 ± 0.127

6.529 ± 0.199

6.031 ± 0.231

6.668 ± 0.187

1.24 ± 0.097

3.907 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski