Amino acid dipepetide frequency for Yersinia phage phiR2-01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.129AlaAla: 9.129 ± 1.282
0.647AlaCys: 0.647 ± 0.159
4.629AlaAsp: 4.629 ± 0.542
6.183AlaGlu: 6.183 ± 0.467
2.881AlaPhe: 2.881 ± 0.309
4.726AlaGly: 4.726 ± 0.569
1.165AlaHis: 1.165 ± 0.225
4.532AlaIle: 4.532 ± 0.407
6.28AlaLys: 6.28 ± 0.523
6.636AlaLeu: 6.636 ± 0.479
2.493AlaMet: 2.493 ± 0.334
3.658AlaAsn: 3.658 ± 0.404
2.59AlaPro: 2.59 ± 0.358
3.011AlaGln: 3.011 ± 0.439
3.399AlaArg: 3.399 ± 0.359
4.759AlaSer: 4.759 ± 0.656
4.403AlaThr: 4.403 ± 0.61
4.662AlaVal: 4.662 ± 0.524
1.23AlaTrp: 1.23 ± 0.221
2.784AlaTyr: 2.784 ± 0.287
0.0AlaXaa: 0.0 ± 0.0
Cys
0.745CysAla: 0.745 ± 0.198
0.259CysCys: 0.259 ± 0.115
0.583CysAsp: 0.583 ± 0.135
0.777CysGlu: 0.777 ± 0.153
0.55CysPhe: 0.55 ± 0.142
0.939CysGly: 0.939 ± 0.192
0.129CysHis: 0.129 ± 0.065
0.647CysIle: 0.647 ± 0.162
0.809CysLys: 0.809 ± 0.171
0.809CysLeu: 0.809 ± 0.169
0.324CysMet: 0.324 ± 0.109
0.388CysAsn: 0.388 ± 0.104
0.421CysPro: 0.421 ± 0.13
0.356CysGln: 0.356 ± 0.125
0.388CysArg: 0.388 ± 0.125
0.745CysSer: 0.745 ± 0.177
0.615CysThr: 0.615 ± 0.157
0.615CysVal: 0.615 ± 0.144
0.065CysTrp: 0.065 ± 0.046
0.291CysTyr: 0.291 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
4.921AspAla: 4.921 ± 0.498
0.712AspCys: 0.712 ± 0.149
3.172AspAsp: 3.172 ± 0.435
4.37AspGlu: 4.37 ± 0.387
2.719AspPhe: 2.719 ± 0.27
3.82AspGly: 3.82 ± 0.402
1.263AspHis: 1.263 ± 0.186
4.467AspIle: 4.467 ± 0.419
4.532AspLys: 4.532 ± 0.392
5.697AspLeu: 5.697 ± 0.497
2.201AspMet: 2.201 ± 0.237
2.654AspAsn: 2.654 ± 0.329
2.557AspPro: 2.557 ± 0.283
1.78AspGln: 1.78 ± 0.244
2.687AspArg: 2.687 ± 0.286
3.658AspSer: 3.658 ± 0.338
3.917AspThr: 3.917 ± 0.353
3.852AspVal: 3.852 ± 0.336
1.101AspTrp: 1.101 ± 0.183
3.172AspTyr: 3.172 ± 0.332
0.0AspXaa: 0.0 ± 0.0
Glu
5.989GluAla: 5.989 ± 0.47
0.68GluCys: 0.68 ± 0.143
3.917GluAsp: 3.917 ± 0.371
5.05GluGlu: 5.05 ± 0.459
2.849GluPhe: 2.849 ± 0.328
3.82GluGly: 3.82 ± 0.417
1.263GluHis: 1.263 ± 0.202
5.115GluIle: 5.115 ± 0.405
4.629GluLys: 4.629 ± 0.386
7.089GluLeu: 7.089 ± 0.542
2.201GluMet: 2.201 ± 0.342
2.137GluAsn: 2.137 ± 0.241
1.716GluPro: 1.716 ± 0.306
2.881GluGln: 2.881 ± 0.377
3.334GluArg: 3.334 ± 0.372
3.593GluSer: 3.593 ± 0.304
4.014GluThr: 4.014 ± 0.326
5.212GluVal: 5.212 ± 0.392
1.133GluTrp: 1.133 ± 0.21
2.881GluTyr: 2.881 ± 0.272
0.0GluXaa: 0.0 ± 0.0
Phe
2.46PheAla: 2.46 ± 0.319
0.421PheCys: 0.421 ± 0.126
2.849PheAsp: 2.849 ± 0.354
2.59PheGlu: 2.59 ± 0.277
1.424PhePhe: 1.424 ± 0.244
3.108PheGly: 3.108 ± 0.319
1.004PheHis: 1.004 ± 0.209
2.59PheIle: 2.59 ± 0.314
2.719PheLys: 2.719 ± 0.238
3.075PheLeu: 3.075 ± 0.325
0.777PheMet: 0.777 ± 0.159
2.622PheAsn: 2.622 ± 0.266
1.845PhePro: 1.845 ± 0.264
1.392PheGln: 1.392 ± 0.233
1.975PheArg: 1.975 ± 0.311
2.946PheSer: 2.946 ± 0.293
2.137PheThr: 2.137 ± 0.245
2.752PheVal: 2.752 ± 0.282
0.356PheTrp: 0.356 ± 0.108
1.683PheTyr: 1.683 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
4.791GlyAla: 4.791 ± 0.512
1.133GlyCys: 1.133 ± 0.223
3.917GlyAsp: 3.917 ± 0.342
4.759GlyGlu: 4.759 ± 0.412
2.849GlyPhe: 2.849 ± 0.305
3.367GlyGly: 3.367 ± 0.351
1.133GlyHis: 1.133 ± 0.219
4.662GlyIle: 4.662 ± 0.356
5.147GlyLys: 5.147 ± 0.336
4.435GlyLeu: 4.435 ± 0.359
2.039GlyMet: 2.039 ± 0.262
3.788GlyAsn: 3.788 ± 0.493
1.036GlyPro: 1.036 ± 0.182
2.007GlyGln: 2.007 ± 0.24
2.687GlyArg: 2.687 ± 0.332
4.467GlySer: 4.467 ± 0.502
4.403GlyThr: 4.403 ± 0.609
4.759GlyVal: 4.759 ± 0.449
0.777GlyTrp: 0.777 ± 0.179
2.978GlyTyr: 2.978 ± 0.341
0.0GlyXaa: 0.0 ± 0.0
His
0.874HisAla: 0.874 ± 0.19
0.259HisCys: 0.259 ± 0.093
1.424HisAsp: 1.424 ± 0.235
1.101HisGlu: 1.101 ± 0.18
0.874HisPhe: 0.874 ± 0.19
1.101HisGly: 1.101 ± 0.193
0.486HisHis: 0.486 ± 0.128
1.263HisIle: 1.263 ± 0.205
1.263HisLys: 1.263 ± 0.196
1.068HisLeu: 1.068 ± 0.2
0.453HisMet: 0.453 ± 0.147
0.777HisAsn: 0.777 ± 0.177
0.874HisPro: 0.874 ± 0.199
0.55HisGln: 0.55 ± 0.164
0.874HisArg: 0.874 ± 0.198
1.489HisSer: 1.489 ± 0.199
0.939HisThr: 0.939 ± 0.206
0.874HisVal: 0.874 ± 0.18
0.129HisTrp: 0.129 ± 0.073
0.906HisTyr: 0.906 ± 0.187
0.0HisXaa: 0.0 ± 0.0
Ile
5.277IleAla: 5.277 ± 0.396
0.809IleCys: 0.809 ± 0.216
4.629IleAsp: 4.629 ± 0.437
4.662IleGlu: 4.662 ± 0.37
2.557IlePhe: 2.557 ± 0.327
4.176IleGly: 4.176 ± 0.361
1.327IleHis: 1.327 ± 0.223
3.788IleIle: 3.788 ± 0.312
4.662IleLys: 4.662 ± 0.377
4.856IleLeu: 4.856 ± 0.442
1.424IleMet: 1.424 ± 0.251
3.755IleAsn: 3.755 ± 0.373
2.622IlePro: 2.622 ± 0.277
2.234IleGln: 2.234 ± 0.221
2.849IleArg: 2.849 ± 0.254
4.467IleSer: 4.467 ± 0.363
4.435IleThr: 4.435 ± 0.393
3.755IleVal: 3.755 ± 0.339
0.745IleTrp: 0.745 ± 0.159
2.137IleTyr: 2.137 ± 0.253
0.0IleXaa: 0.0 ± 0.0
Lys
6.345LysAla: 6.345 ± 0.576
0.615LysCys: 0.615 ± 0.158
4.791LysAsp: 4.791 ± 0.406
4.985LysGlu: 4.985 ± 0.387
2.849LysPhe: 2.849 ± 0.255
3.334LysGly: 3.334 ± 0.349
0.939LysHis: 0.939 ± 0.192
3.464LysIle: 3.464 ± 0.386
3.755LysLys: 3.755 ± 0.494
6.895LysLeu: 6.895 ± 0.58
2.59LysMet: 2.59 ± 0.277
3.529LysAsn: 3.529 ± 0.436
2.298LysPro: 2.298 ± 0.318
2.816LysGln: 2.816 ± 0.387
3.431LysArg: 3.431 ± 0.303
4.403LysSer: 4.403 ± 0.384
3.949LysThr: 3.949 ± 0.348
4.953LysVal: 4.953 ± 0.375
0.647LysTrp: 0.647 ± 0.166
2.978LysTyr: 2.978 ± 0.321
0.0LysXaa: 0.0 ± 0.0
Leu
6.377LeuAla: 6.377 ± 0.488
0.647LeuCys: 0.647 ± 0.147
5.892LeuAsp: 5.892 ± 0.438
6.41LeuGlu: 6.41 ± 0.523
2.784LeuPhe: 2.784 ± 0.298
5.406LeuGly: 5.406 ± 0.411
1.942LeuHis: 1.942 ± 0.275
4.694LeuIle: 4.694 ± 0.359
5.212LeuLys: 5.212 ± 0.45
6.571LeuLeu: 6.571 ± 0.467
2.298LeuMet: 2.298 ± 0.302
5.115LeuAsn: 5.115 ± 0.453
3.205LeuPro: 3.205 ± 0.377
3.27LeuGln: 3.27 ± 0.343
3.27LeuArg: 3.27 ± 0.308
4.856LeuSer: 4.856 ± 0.446
4.921LeuThr: 4.921 ± 0.395
5.633LeuVal: 5.633 ± 0.451
0.647LeuTrp: 0.647 ± 0.157
2.622LeuTyr: 2.622 ± 0.295
0.0LeuXaa: 0.0 ± 0.0
Met
1.942MetAla: 1.942 ± 0.335
0.291MetCys: 0.291 ± 0.086
1.36MetAsp: 1.36 ± 0.19
1.975MetGlu: 1.975 ± 0.272
1.004MetPhe: 1.004 ± 0.2
1.554MetGly: 1.554 ± 0.249
0.421MetHis: 0.421 ± 0.121
1.975MetIle: 1.975 ± 0.26
2.266MetLys: 2.266 ± 0.322
2.201MetLeu: 2.201 ± 0.292
0.453MetMet: 0.453 ± 0.106
1.295MetAsn: 1.295 ± 0.19
1.133MetPro: 1.133 ± 0.19
1.133MetGln: 1.133 ± 0.242
1.068MetArg: 1.068 ± 0.184
2.525MetSer: 2.525 ± 0.275
2.072MetThr: 2.072 ± 0.221
1.489MetVal: 1.489 ± 0.224
0.291MetTrp: 0.291 ± 0.102
0.809MetTyr: 0.809 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
4.046AsnAla: 4.046 ± 0.465
0.388AsnCys: 0.388 ± 0.097
2.849AsnAsp: 2.849 ± 0.298
2.719AsnGlu: 2.719 ± 0.346
1.845AsnPhe: 1.845 ± 0.252
4.208AsnGly: 4.208 ± 0.477
0.583AsnHis: 0.583 ± 0.132
3.658AsnIle: 3.658 ± 0.262
3.723AsnLys: 3.723 ± 0.381
4.144AsnLeu: 4.144 ± 0.318
1.133AsnMet: 1.133 ± 0.185
2.428AsnAsn: 2.428 ± 0.286
2.493AsnPro: 2.493 ± 0.31
1.554AsnGln: 1.554 ± 0.207
2.396AsnArg: 2.396 ± 0.313
3.917AsnSer: 3.917 ± 0.355
2.59AsnThr: 2.59 ± 0.268
3.755AsnVal: 3.755 ± 0.319
0.55AsnTrp: 0.55 ± 0.139
2.072AsnTyr: 2.072 ± 0.264
0.0AsnXaa: 0.0 ± 0.0
Pro
2.622ProAla: 2.622 ± 0.284
0.291ProCys: 0.291 ± 0.093
2.525ProAsp: 2.525 ± 0.318
3.302ProGlu: 3.302 ± 0.363
1.78ProPhe: 1.78 ± 0.247
2.104ProGly: 2.104 ± 0.268
0.712ProHis: 0.712 ± 0.146
2.428ProIle: 2.428 ± 0.293
2.234ProLys: 2.234 ± 0.253
2.266ProLeu: 2.266 ± 0.241
0.55ProMet: 0.55 ± 0.151
2.039ProAsn: 2.039 ± 0.229
1.392ProPro: 1.392 ± 0.22
0.809ProGln: 0.809 ± 0.136
1.554ProArg: 1.554 ± 0.207
1.716ProSer: 1.716 ± 0.212
2.072ProThr: 2.072 ± 0.226
2.752ProVal: 2.752 ± 0.246
0.518ProTrp: 0.518 ± 0.124
1.586ProTyr: 1.586 ± 0.268
0.0ProXaa: 0.0 ± 0.0
Gln
3.302GlnAla: 3.302 ± 0.573
0.324GlnCys: 0.324 ± 0.099
2.331GlnAsp: 2.331 ± 0.322
2.654GlnGlu: 2.654 ± 0.363
1.521GlnPhe: 1.521 ± 0.213
2.201GlnGly: 2.201 ± 0.243
0.486GlnHis: 0.486 ± 0.133
2.169GlnIle: 2.169 ± 0.211
2.525GlnLys: 2.525 ± 0.338
3.14GlnLeu: 3.14 ± 0.412
1.004GlnMet: 1.004 ± 0.19
1.586GlnAsn: 1.586 ± 0.235
0.712GlnPro: 0.712 ± 0.168
1.845GlnGln: 1.845 ± 0.354
1.619GlnArg: 1.619 ± 0.249
2.331GlnSer: 2.331 ± 0.24
1.845GlnThr: 1.845 ± 0.308
2.201GlnVal: 2.201 ± 0.219
0.583GlnTrp: 0.583 ± 0.155
1.36GlnTyr: 1.36 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
3.367ArgAla: 3.367 ± 0.363
0.194ArgCys: 0.194 ± 0.069
3.075ArgAsp: 3.075 ± 0.293
3.108ArgGlu: 3.108 ± 0.345
1.91ArgPhe: 1.91 ± 0.26
3.205ArgGly: 3.205 ± 0.287
0.647ArgHis: 0.647 ± 0.157
2.752ArgIle: 2.752 ± 0.345
2.946ArgLys: 2.946 ± 0.386
3.561ArgLeu: 3.561 ± 0.383
1.295ArgMet: 1.295 ± 0.181
2.622ArgAsn: 2.622 ± 0.266
1.392ArgPro: 1.392 ± 0.193
1.36ArgGln: 1.36 ± 0.229
2.201ArgArg: 2.201 ± 0.31
2.687ArgSer: 2.687 ± 0.332
2.687ArgThr: 2.687 ± 0.315
3.108ArgVal: 3.108 ± 0.298
0.324ArgTrp: 0.324 ± 0.108
1.554ArgTyr: 1.554 ± 0.275
0.0ArgXaa: 0.0 ± 0.0
Ser
4.597SerAla: 4.597 ± 0.647
0.939SerCys: 0.939 ± 0.174
3.205SerAsp: 3.205 ± 0.307
3.723SerGlu: 3.723 ± 0.337
2.978SerPhe: 2.978 ± 0.277
5.244SerGly: 5.244 ± 0.335
1.036SerHis: 1.036 ± 0.163
4.823SerIle: 4.823 ± 0.449
4.5SerLys: 4.5 ± 0.421
5.536SerLeu: 5.536 ± 0.375
1.619SerMet: 1.619 ± 0.23
3.529SerAsn: 3.529 ± 0.3
2.072SerPro: 2.072 ± 0.31
2.201SerGln: 2.201 ± 0.28
2.816SerArg: 2.816 ± 0.319
4.564SerSer: 4.564 ± 0.506
3.723SerThr: 3.723 ± 0.389
4.046SerVal: 4.046 ± 0.395
1.295SerTrp: 1.295 ± 0.211
2.849SerTyr: 2.849 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
4.953ThrAla: 4.953 ± 0.556
0.453ThrCys: 0.453 ± 0.102
3.399ThrAsp: 3.399 ± 0.311
3.529ThrGlu: 3.529 ± 0.416
2.654ThrPhe: 2.654 ± 0.275
5.568ThrGly: 5.568 ± 0.658
1.036ThrHis: 1.036 ± 0.238
4.273ThrIle: 4.273 ± 0.444
4.014ThrLys: 4.014 ± 0.375
4.305ThrLeu: 4.305 ± 0.343
1.424ThrMet: 1.424 ± 0.214
3.334ThrAsn: 3.334 ± 0.31
2.039ThrPro: 2.039 ± 0.266
2.234ThrGln: 2.234 ± 0.243
2.072ThrArg: 2.072 ± 0.24
4.144ThrSer: 4.144 ± 0.374
3.982ThrThr: 3.982 ± 0.522
4.5ThrVal: 4.5 ± 0.493
0.518ThrTrp: 0.518 ± 0.119
1.878ThrTyr: 1.878 ± 0.224
0.0ThrXaa: 0.0 ± 0.0
Val
5.05ValAla: 5.05 ± 0.364
0.453ValCys: 0.453 ± 0.123
4.856ValAsp: 4.856 ± 0.375
4.629ValGlu: 4.629 ± 0.381
2.525ValPhe: 2.525 ± 0.279
3.949ValGly: 3.949 ± 0.346
1.004ValHis: 1.004 ± 0.191
4.467ValIle: 4.467 ± 0.341
4.435ValLys: 4.435 ± 0.376
5.277ValLeu: 5.277 ± 0.345
1.619ValMet: 1.619 ± 0.217
2.849ValAsn: 2.849 ± 0.355
2.978ValPro: 2.978 ± 0.305
2.363ValGln: 2.363 ± 0.251
2.849ValArg: 2.849 ± 0.299
4.726ValSer: 4.726 ± 0.423
4.564ValThr: 4.564 ± 0.496
4.532ValVal: 4.532 ± 0.465
0.745ValTrp: 0.745 ± 0.165
2.816ValTyr: 2.816 ± 0.332
0.0ValXaa: 0.0 ± 0.0
Trp
0.55TrpAla: 0.55 ± 0.132
0.227TrpCys: 0.227 ± 0.09
0.971TrpAsp: 0.971 ± 0.175
0.809TrpGlu: 0.809 ± 0.147
0.55TrpPhe: 0.55 ± 0.131
0.712TrpGly: 0.712 ± 0.146
0.227TrpHis: 0.227 ± 0.093
0.583TrpIle: 0.583 ± 0.142
1.101TrpLys: 1.101 ± 0.168
1.392TrpLeu: 1.392 ± 0.243
0.356TrpMet: 0.356 ± 0.119
0.583TrpAsn: 0.583 ± 0.16
0.453TrpPro: 0.453 ± 0.119
0.518TrpGln: 0.518 ± 0.128
0.68TrpArg: 0.68 ± 0.165
0.777TrpSer: 0.777 ± 0.225
0.486TrpThr: 0.486 ± 0.163
0.939TrpVal: 0.939 ± 0.201
0.162TrpTrp: 0.162 ± 0.072
0.324TrpTyr: 0.324 ± 0.104
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.46TyrAla: 2.46 ± 0.281
0.712TyrCys: 0.712 ± 0.18
2.881TyrAsp: 2.881 ± 0.287
2.201TyrGlu: 2.201 ± 0.342
1.619TyrPhe: 1.619 ± 0.24
2.59TyrGly: 2.59 ± 0.331
0.777TyrHis: 0.777 ± 0.15
2.816TyrIle: 2.816 ± 0.298
2.816TyrLys: 2.816 ± 0.289
2.816TyrLeu: 2.816 ± 0.301
0.971TyrMet: 0.971 ± 0.206
2.363TyrAsn: 2.363 ± 0.278
1.424TyrPro: 1.424 ± 0.213
1.424TyrGln: 1.424 ± 0.257
1.845TyrArg: 1.845 ± 0.252
2.525TyrSer: 2.525 ± 0.239
2.557TyrThr: 2.557 ± 0.286
2.298TyrVal: 2.298 ± 0.263
0.583TyrTrp: 0.583 ± 0.146
1.392TyrTyr: 1.392 ± 0.211
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 154 proteins (30892 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski