Mycobacterium avium subsp. avium 2285 (R)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium avium complex (MAC); Mycobacterium avium; Mycobacterium avium subsp. avium

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X8B2B9|X8B2B9_MYCAV Putative transmembrane protein OS=Mycobacterium avium subsp. avium 2285 (R) OX=1299330 GN=I549_5181 PE=4 SV=1
MM1 pKa = 7.42GHH3 pKa = 6.41AQCFAMAPDD12 pKa = 5.02LFPIDD17 pKa = 3.91DD18 pKa = 4.48LGYY21 pKa = 8.68STLEE25 pKa = 3.86EE26 pKa = 4.41HH27 pKa = 6.68EE28 pKa = 4.71VAPEE32 pKa = 3.88DD33 pKa = 4.3QPATRR38 pKa = 11.84DD39 pKa = 3.47GVAACPEE46 pKa = 3.96RR47 pKa = 11.84ALILDD52 pKa = 4.65DD53 pKa = 4.92DD54 pKa = 4.82ASS56 pKa = 3.49

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X8AYR0|X8AYR0_MYCAV Cytochrome P450 family protein OS=Mycobacterium avium subsp. avium 2285 (R) OX=1299330 GN=I549_5726 PE=3 SV=1
MM1 pKa = 7.6AGHH4 pKa = 6.84RR5 pKa = 11.84QRR7 pKa = 11.84FAQRR11 pKa = 11.84NFRR14 pKa = 11.84QRR16 pKa = 11.84PPGAGGRR23 pKa = 11.84DD24 pKa = 3.78PRR26 pKa = 11.84RR27 pKa = 11.84VRR29 pKa = 11.84HH30 pKa = 4.62QHH32 pKa = 5.93RR33 pKa = 11.84KK34 pKa = 7.53PGRR37 pKa = 11.84PAADD41 pKa = 3.58LRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84PPPRR49 pKa = 11.84HH50 pKa = 5.63GRR52 pKa = 11.84TPAPHH57 pKa = 6.88RR58 pKa = 11.84VDD60 pKa = 3.27GHH62 pKa = 6.08PGGRR66 pKa = 11.84PPARR70 pKa = 11.84TGGLVGPTGAAGRR83 pKa = 11.84RR84 pKa = 11.84PPRR87 pKa = 11.84RR88 pKa = 11.84PGAPQLGRR96 pKa = 11.84ATAAPAARR104 pKa = 11.84AAPRR108 pKa = 11.84PPARR112 pKa = 11.84PCTAAAHH119 pKa = 6.08PAATRR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84AAPPPVAPARR136 pKa = 11.84RR137 pKa = 11.84PRR139 pKa = 11.84TPRR142 pKa = 11.84WRR144 pKa = 11.84PRR146 pKa = 11.84LPSRR150 pKa = 11.84PRR152 pKa = 11.84WPTWRR157 pKa = 11.84PRR159 pKa = 11.84CSRR162 pKa = 11.84RR163 pKa = 3.26

Molecular weight: 18.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6021

0

6021

1547435

37

2227

257.0

27.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.27 ± 0.05

0.98 ± 0.011

6.098 ± 0.024

5.015 ± 0.032

2.933 ± 0.021

8.856 ± 0.031

2.291 ± 0.016

4.062 ± 0.02

2.147 ± 0.023

9.592 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.055 ± 0.013

2.201 ± 0.016

6.19 ± 0.029

3.043 ± 0.018

8.055 ± 0.034

5.399 ± 0.02

5.859 ± 0.022

8.238 ± 0.031

1.602 ± 0.015

2.114 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski