Tortoise microvirus 18

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W5N7|A0A4P8W5N7_9VIRU Uncharacterized protein OS=Tortoise microvirus 18 OX=2583119 PE=4 SV=1
MM1 pKa = 7.47EE2 pKa = 5.78NYY4 pKa = 9.74TEE6 pKa = 4.89GLHH9 pKa = 5.81KK10 pKa = 10.71QNSSEE15 pKa = 3.86EE16 pKa = 3.82RR17 pKa = 11.84VEE19 pKa = 3.87IVKK22 pKa = 10.43KK23 pKa = 11.09VEE25 pKa = 3.9GTPFSIISLDD35 pKa = 3.97DD36 pKa = 3.6GCKK39 pKa = 9.94ISIGTAIASEE49 pKa = 4.18IVFTDD54 pKa = 3.55EE55 pKa = 4.3KK56 pKa = 11.0QATEE60 pKa = 4.29YY61 pKa = 10.68IKK63 pKa = 11.11GEE65 pKa = 4.36YY66 pKa = 7.89GTQWDD71 pKa = 4.03LLGTLMYY78 pKa = 10.83VITNMNKK85 pKa = 10.04GEE87 pKa = 3.97

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W5V5|A0A4P8W5V5_9VIRU Uncharacterized protein OS=Tortoise microvirus 18 OX=2583119 PE=4 SV=1
MM1 pKa = 6.93YY2 pKa = 10.31KK3 pKa = 8.93KK4 pKa = 8.76TRR6 pKa = 11.84RR7 pKa = 11.84NSRR10 pKa = 11.84IFRR13 pKa = 11.84RR14 pKa = 11.84TEE16 pKa = 3.82YY17 pKa = 10.53IDD19 pKa = 3.61IEE21 pKa = 4.23TGEE24 pKa = 3.98TSEE27 pKa = 4.81RR28 pKa = 11.84IRR30 pKa = 11.84GEE32 pKa = 3.96YY33 pKa = 9.58FFKK36 pKa = 10.81EE37 pKa = 3.56EE38 pKa = 3.56RR39 pKa = 11.84AEE41 pKa = 3.71IRR43 pKa = 11.84EE44 pKa = 4.0YY45 pKa = 9.1EE46 pKa = 4.03TEE48 pKa = 4.11YY49 pKa = 11.16NKK51 pKa = 10.53TIITTKK57 pKa = 9.83YY58 pKa = 9.32GKK60 pKa = 9.02LHH62 pKa = 6.73RR63 pKa = 11.84RR64 pKa = 11.84PTQTKK69 pKa = 9.97LFF71 pKa = 4.16

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1724

69

676

215.5

24.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.555 ± 1.548

0.406 ± 0.277

4.118 ± 0.372

10.035 ± 1.411

2.958 ± 0.403

7.599 ± 1.15

0.986 ± 0.221

7.077 ± 0.617

8.701 ± 1.918

7.019 ± 0.432

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.572

6.729 ± 0.491

2.32 ± 0.441

4.118 ± 0.732

5.162 ± 0.857

5.858 ± 0.651

7.599 ± 0.785

3.944 ± 0.947

1.508 ± 0.248

4.582 ± 0.418
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski