Amino acid dipepetide frequency for Verrucomicrobiaceae bacterium SCGC AG-212-N21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.089AlaAla: 13.089 ± 0.245
1.149AlaCys: 1.149 ± 0.051
5.661AlaAsp: 5.661 ± 0.133
6.52AlaGlu: 6.52 ± 0.155
3.776AlaPhe: 3.776 ± 0.102
8.761AlaGly: 8.761 ± 0.201
2.182AlaHis: 2.182 ± 0.078
4.676AlaIle: 4.676 ± 0.114
5.18AlaLys: 5.18 ± 0.143
10.92AlaLeu: 10.92 ± 0.194
2.918AlaMet: 2.918 ± 0.079
3.011AlaAsn: 3.011 ± 0.113
5.347AlaPro: 5.347 ± 0.166
3.942AlaGln: 3.942 ± 0.108
6.455AlaArg: 6.455 ± 0.176
6.283AlaSer: 6.283 ± 0.14
6.122AlaThr: 6.122 ± 0.144
7.378AlaVal: 7.378 ± 0.135
1.703AlaTrp: 1.703 ± 0.065
2.14AlaTyr: 2.14 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
1.051CysAla: 1.051 ± 0.052
0.164CysCys: 0.164 ± 0.024
0.531CysAsp: 0.531 ± 0.04
0.637CysGlu: 0.637 ± 0.044
0.447CysPhe: 0.447 ± 0.036
1.064CysGly: 1.064 ± 0.061
0.361CysHis: 0.361 ± 0.036
0.468CysIle: 0.468 ± 0.035
0.322CysLys: 0.322 ± 0.03
1.144CysLeu: 1.144 ± 0.063
0.239CysMet: 0.239 ± 0.023
0.294CysAsn: 0.294 ± 0.026
0.447CysPro: 0.447 ± 0.038
0.369CysGln: 0.369 ± 0.034
0.663CysArg: 0.663 ± 0.043
0.601CysSer: 0.601 ± 0.044
0.531CysThr: 0.531 ± 0.037
0.832CysVal: 0.832 ± 0.043
0.182CysTrp: 0.182 ± 0.023
0.33CysTyr: 0.33 ± 0.031
0.0CysXaa: 0.0 ± 0.0
Asp
6.348AspAla: 6.348 ± 0.133
0.564AspCys: 0.564 ± 0.044
2.92AspAsp: 2.92 ± 0.08
3.298AspGlu: 3.298 ± 0.093
2.356AspPhe: 2.356 ± 0.074
5.126AspGly: 5.126 ± 0.16
1.266AspHis: 1.266 ± 0.066
2.289AspIle: 2.289 ± 0.089
2.421AspLys: 2.421 ± 0.09
5.308AspLeu: 5.308 ± 0.135
1.097AspMet: 1.097 ± 0.055
1.303AspAsn: 1.303 ± 0.065
3.105AspPro: 3.105 ± 0.094
1.594AspGln: 1.594 ± 0.057
3.131AspArg: 3.131 ± 0.095
2.64AspSer: 2.64 ± 0.101
2.824AspThr: 2.824 ± 0.09
3.963AspVal: 3.963 ± 0.101
1.051AspTrp: 1.051 ± 0.052
1.638AspTyr: 1.638 ± 0.067
0.0AspXaa: 0.0 ± 0.0
Glu
6.462GluAla: 6.462 ± 0.15
0.541GluCys: 0.541 ± 0.037
2.751GluAsp: 2.751 ± 0.095
3.526GluGlu: 3.526 ± 0.106
2.408GluPhe: 2.408 ± 0.08
4.39GluGly: 4.39 ± 0.12
1.508GluHis: 1.508 ± 0.072
2.754GluIle: 2.754 ± 0.084
3.5GluLys: 3.5 ± 0.122
5.724GluLeu: 5.724 ± 0.145
1.646GluMet: 1.646 ± 0.062
1.807GluAsn: 1.807 ± 0.067
2.853GluPro: 2.853 ± 0.099
2.276GluGln: 2.276 ± 0.08
4.099GluArg: 4.099 ± 0.132
3.123GluSer: 3.123 ± 0.097
2.996GluThr: 2.996 ± 0.079
4.2GluVal: 4.2 ± 0.102
1.001GluTrp: 1.001 ± 0.053
1.287GluTyr: 1.287 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
4.26PheAla: 4.26 ± 0.108
0.523PheCys: 0.523 ± 0.038
2.473PheAsp: 2.473 ± 0.086
2.153PheGlu: 2.153 ± 0.07
1.729PhePhe: 1.729 ± 0.083
3.298PheGly: 3.298 ± 0.09
0.897PheHis: 0.897 ± 0.044
1.797PheIle: 1.797 ± 0.068
1.454PheLys: 1.454 ± 0.068
3.781PheLeu: 3.781 ± 0.11
0.835PheMet: 0.835 ± 0.046
1.285PheAsn: 1.285 ± 0.053
1.727PhePro: 1.727 ± 0.064
1.136PheGln: 1.136 ± 0.053
2.385PheArg: 2.385 ± 0.087
2.608PheSer: 2.608 ± 0.098
2.642PheThr: 2.642 ± 0.109
2.827PheVal: 2.827 ± 0.096
0.59PheTrp: 0.59 ± 0.042
1.074PheTyr: 1.074 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
7.914GlyAla: 7.914 ± 0.205
0.944GlyCys: 0.944 ± 0.044
4.483GlyAsp: 4.483 ± 0.113
4.32GlyGlu: 4.32 ± 0.107
3.461GlyPhe: 3.461 ± 0.097
7.178GlyGly: 7.178 ± 0.234
2.023GlyHis: 2.023 ± 0.084
4.127GlyIle: 4.127 ± 0.1
4.46GlyLys: 4.46 ± 0.137
7.071GlyLeu: 7.071 ± 0.168
2.104GlyMet: 2.104 ± 0.076
2.705GlyAsn: 2.705 ± 0.113
3.155GlyPro: 3.155 ± 0.104
2.772GlyGln: 2.772 ± 0.085
4.863GlyArg: 4.863 ± 0.12
4.959GlySer: 4.959 ± 0.159
5.074GlyThr: 5.074 ± 0.222
5.922GlyVal: 5.922 ± 0.129
1.534GlyTrp: 1.534 ± 0.066
2.364GlyTyr: 2.364 ± 0.072
0.0GlyXaa: 0.0 ± 0.0
His
2.564HisAla: 2.564 ± 0.094
0.286HisCys: 0.286 ± 0.031
1.399HisAsp: 1.399 ± 0.063
1.436HisGlu: 1.436 ± 0.063
1.064HisPhe: 1.064 ± 0.053
2.182HisGly: 2.182 ± 0.086
0.765HisHis: 0.765 ± 0.042
0.861HisIle: 0.861 ± 0.051
0.739HisLys: 0.739 ± 0.044
2.335HisLeu: 2.335 ± 0.094
0.476HisMet: 0.476 ± 0.032
0.57HisAsn: 0.57 ± 0.034
1.698HisPro: 1.698 ± 0.074
0.759HisGln: 0.759 ± 0.046
1.683HisArg: 1.683 ± 0.078
1.253HisSer: 1.253 ± 0.054
1.123HisThr: 1.123 ± 0.06
1.75HisVal: 1.75 ± 0.061
0.497HisTrp: 0.497 ± 0.039
0.668HisTyr: 0.668 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
5.297IleAla: 5.297 ± 0.121
0.473IleCys: 0.473 ± 0.034
2.348IleAsp: 2.348 ± 0.073
3.011IleGlu: 3.011 ± 0.091
1.586IlePhe: 1.586 ± 0.066
3.786IleGly: 3.786 ± 0.127
1.079IleHis: 1.079 ± 0.055
2.047IleIle: 2.047 ± 0.076
1.87IleLys: 1.87 ± 0.078
3.953IleLeu: 3.953 ± 0.1
0.837IleMet: 0.837 ± 0.043
1.342IleAsn: 1.342 ± 0.066
2.278IlePro: 2.278 ± 0.064
1.251IleGln: 1.251 ± 0.055
2.585IleArg: 2.585 ± 0.092
2.632IleSer: 2.632 ± 0.081
2.91IleThr: 2.91 ± 0.095
3.389IleVal: 3.389 ± 0.091
0.596IleTrp: 0.596 ± 0.038
1.152IleTyr: 1.152 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
4.728LysAla: 4.728 ± 0.143
0.338LysCys: 0.338 ± 0.029
2.585LysAsp: 2.585 ± 0.11
2.907LysGlu: 2.907 ± 0.099
1.436LysPhe: 1.436 ± 0.053
3.292LysGly: 3.292 ± 0.116
1.196LysHis: 1.196 ± 0.063
1.794LysIle: 1.794 ± 0.07
2.416LysLys: 2.416 ± 0.113
4.923LysLeu: 4.923 ± 0.118
1.123LysMet: 1.123 ± 0.056
1.397LysAsn: 1.397 ± 0.06
3.251LysPro: 3.251 ± 0.098
1.667LysGln: 1.667 ± 0.068
2.874LysArg: 2.874 ± 0.097
2.533LysSer: 2.533 ± 0.096
2.447LysThr: 2.447 ± 0.085
3.207LysVal: 3.207 ± 0.088
0.668LysTrp: 0.668 ± 0.045
0.775LysTyr: 0.775 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
11.484LeuAla: 11.484 ± 0.21
1.337LeuCys: 1.337 ± 0.063
5.516LeuAsp: 5.516 ± 0.133
5.253LeuGlu: 5.253 ± 0.142
3.578LeuPhe: 3.578 ± 0.105
7.547LeuGly: 7.547 ± 0.16
2.4LeuHis: 2.4 ± 0.092
4.184LeuIle: 4.184 ± 0.111
4.416LeuLys: 4.416 ± 0.134
9.817LeuLeu: 9.817 ± 0.214
1.982LeuMet: 1.982 ± 0.077
3.022LeuAsn: 3.022 ± 0.096
5.518LeuPro: 5.518 ± 0.107
3.321LeuGln: 3.321 ± 0.098
6.931LeuArg: 6.931 ± 0.162
5.89LeuSer: 5.89 ± 0.126
5.747LeuThr: 5.747 ± 0.13
6.834LeuVal: 6.834 ± 0.143
1.542LeuTrp: 1.542 ± 0.076
2.179LeuTyr: 2.179 ± 0.094
0.0LeuXaa: 0.0 ± 0.0
Met
2.255MetAla: 2.255 ± 0.08
0.182MetCys: 0.182 ± 0.023
1.139MetAsp: 1.139 ± 0.049
1.256MetGlu: 1.256 ± 0.061
0.723MetPhe: 0.723 ± 0.044
1.576MetGly: 1.576 ± 0.068
0.614MetHis: 0.614 ± 0.04
1.035MetIle: 1.035 ± 0.053
1.633MetLys: 1.633 ± 0.065
2.289MetLeu: 2.289 ± 0.081
0.572MetMet: 0.572 ± 0.04
1.035MetAsn: 1.035 ± 0.051
1.472MetPro: 1.472 ± 0.069
0.991MetGln: 0.991 ± 0.049
1.755MetArg: 1.755 ± 0.07
1.56MetSer: 1.56 ± 0.063
1.493MetThr: 1.493 ± 0.064
1.602MetVal: 1.602 ± 0.063
0.322MetTrp: 0.322 ± 0.029
0.27MetTyr: 0.27 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
3.095AsnAla: 3.095 ± 0.096
0.299AsnCys: 0.299 ± 0.026
1.706AsnAsp: 1.706 ± 0.063
1.584AsnGlu: 1.584 ± 0.062
1.248AsnPhe: 1.248 ± 0.057
2.978AsnGly: 2.978 ± 0.127
0.788AsnHis: 0.788 ± 0.044
1.326AsnIle: 1.326 ± 0.07
1.043AsnLys: 1.043 ± 0.06
2.967AsnLeu: 2.967 ± 0.097
0.622AsnMet: 0.622 ± 0.039
1.082AsnAsn: 1.082 ± 0.07
2.182AsnPro: 2.182 ± 0.083
0.978AsnGln: 0.978 ± 0.057
1.698AsnArg: 1.698 ± 0.064
1.709AsnSer: 1.709 ± 0.087
1.755AsnThr: 1.755 ± 0.106
2.166AsnVal: 2.166 ± 0.087
0.538AsnTrp: 0.538 ± 0.041
0.897AsnTyr: 0.897 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
6.291ProAla: 6.291 ± 0.165
0.364ProCys: 0.364 ± 0.029
3.552ProAsp: 3.552 ± 0.116
3.867ProGlu: 3.867 ± 0.105
2.127ProPhe: 2.127 ± 0.073
4.455ProGly: 4.455 ± 0.125
1.24ProHis: 1.24 ± 0.06
1.937ProIle: 1.937 ± 0.068
2.593ProLys: 2.593 ± 0.089
4.806ProLeu: 4.806 ± 0.115
1.373ProMet: 1.373 ± 0.061
1.677ProAsn: 1.677 ± 0.064
3.368ProPro: 3.368 ± 0.136
1.963ProGln: 1.963 ± 0.073
2.814ProArg: 2.814 ± 0.092
3.292ProSer: 3.292 ± 0.086
2.91ProThr: 2.91 ± 0.108
4.104ProVal: 4.104 ± 0.108
0.835ProTrp: 0.835 ± 0.049
1.24ProTyr: 1.24 ± 0.061
0.0ProXaa: 0.0 ± 0.0
Gln
3.612GlnAla: 3.612 ± 0.111
0.317GlnCys: 0.317 ± 0.031
1.501GlnAsp: 1.501 ± 0.063
2.008GlnGlu: 2.008 ± 0.076
1.303GlnPhe: 1.303 ± 0.051
2.564GlnGly: 2.564 ± 0.08
0.866GlnHis: 0.866 ± 0.048
1.558GlnIle: 1.558 ± 0.059
1.576GlnLys: 1.576 ± 0.081
3.199GlnLeu: 3.199 ± 0.083
0.926GlnMet: 0.926 ± 0.052
1.084GlnAsn: 1.084 ± 0.057
2.104GlnPro: 2.104 ± 0.069
1.464GlnGln: 1.464 ± 0.069
2.715GlnArg: 2.715 ± 0.089
1.948GlnSer: 1.948 ± 0.07
1.768GlnThr: 1.768 ± 0.073
2.504GlnVal: 2.504 ± 0.083
0.606GlnTrp: 0.606 ± 0.039
0.645GlnTyr: 0.645 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
5.919ArgAla: 5.919 ± 0.136
0.694ArgCys: 0.694 ± 0.052
3.581ArgAsp: 3.581 ± 0.102
4.491ArgGlu: 4.491 ± 0.148
2.887ArgPhe: 2.887 ± 0.09
4.473ArgGly: 4.473 ± 0.115
1.529ArgHis: 1.529 ± 0.067
3.269ArgIle: 3.269 ± 0.1
2.588ArgLys: 2.588 ± 0.095
6.384ArgLeu: 6.384 ± 0.138
1.75ArgMet: 1.75 ± 0.066
1.901ArgAsn: 1.901 ± 0.075
3.074ArgPro: 3.074 ± 0.091
2.075ArgGln: 2.075 ± 0.076
4.286ArgArg: 4.286 ± 0.146
3.42ArgSer: 3.42 ± 0.112
3.142ArgThr: 3.142 ± 0.1
4.66ArgVal: 4.66 ± 0.117
1.384ArgTrp: 1.384 ± 0.068
1.787ArgTyr: 1.787 ± 0.073
0.0ArgXaa: 0.0 ± 0.0
Ser
6.062SerAla: 6.062 ± 0.16
0.505SerCys: 0.505 ± 0.037
3.222SerAsp: 3.222 ± 0.088
3.095SerGlu: 3.095 ± 0.085
2.413SerPhe: 2.413 ± 0.08
5.443SerGly: 5.443 ± 0.181
1.459SerHis: 1.459 ± 0.058
2.621SerIle: 2.621 ± 0.09
2.341SerLys: 2.341 ± 0.095
6.08SerLeu: 6.08 ± 0.137
1.358SerMet: 1.358 ± 0.06
1.755SerAsn: 1.755 ± 0.086
3.386SerPro: 3.386 ± 0.102
2.031SerGln: 2.031 ± 0.076
3.578SerArg: 3.578 ± 0.096
3.594SerSer: 3.594 ± 0.117
3.214SerThr: 3.214 ± 0.109
3.981SerVal: 3.981 ± 0.12
0.931SerTrp: 0.931 ± 0.049
1.381SerTyr: 1.381 ± 0.076
0.0SerXaa: 0.0 ± 0.0
Thr
5.916ThrAla: 5.916 ± 0.151
0.538ThrCys: 0.538 ± 0.04
2.931ThrAsp: 2.931 ± 0.101
2.996ThrGlu: 2.996 ± 0.086
2.208ThrPhe: 2.208 ± 0.076
5.225ThrGly: 5.225 ± 0.186
1.253ThrHis: 1.253 ± 0.059
2.601ThrIle: 2.601 ± 0.091
2.216ThrLys: 2.216 ± 0.071
6.317ThrLeu: 6.317 ± 0.159
1.261ThrMet: 1.261 ± 0.063
1.62ThrAsn: 1.62 ± 0.078
3.81ThrPro: 3.81 ± 0.121
1.742ThrGln: 1.742 ± 0.062
3.217ThrArg: 3.217 ± 0.096
3.37ThrSer: 3.37 ± 0.112
3.404ThrThr: 3.404 ± 0.15
4.4ThrVal: 4.4 ± 0.151
1.019ThrTrp: 1.019 ± 0.058
1.355ThrTyr: 1.355 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
7.331ValAla: 7.331 ± 0.142
0.921ValCys: 0.921 ± 0.055
3.651ValAsp: 3.651 ± 0.102
4.275ValGlu: 4.275 ± 0.112
2.853ValPhe: 2.853 ± 0.085
4.957ValGly: 4.957 ± 0.145
1.56ValHis: 1.56 ± 0.068
3.459ValIle: 3.459 ± 0.1
3.115ValLys: 3.115 ± 0.1
7.313ValLeu: 7.313 ± 0.141
1.729ValMet: 1.729 ± 0.064
2.335ValAsn: 2.335 ± 0.091
4.057ValPro: 4.057 ± 0.105
2.226ValGln: 2.226 ± 0.083
4.551ValArg: 4.551 ± 0.133
4.616ValSer: 4.616 ± 0.124
4.827ValThr: 4.827 ± 0.136
5.511ValVal: 5.511 ± 0.126
1.165ValTrp: 1.165 ± 0.058
1.537ValTyr: 1.537 ± 0.072
0.0ValXaa: 0.0 ± 0.0
Trp
1.243TrpAla: 1.243 ± 0.058
0.255TrpCys: 0.255 ± 0.029
0.889TrpAsp: 0.889 ± 0.047
0.775TrpGlu: 0.775 ± 0.048
0.746TrpPhe: 0.746 ± 0.05
1.045TrpGly: 1.045 ± 0.052
0.531TrpHis: 0.531 ± 0.035
0.819TrpIle: 0.819 ± 0.052
0.876TrpLys: 0.876 ± 0.048
1.917TrpLeu: 1.917 ± 0.076
0.479TrpMet: 0.479 ± 0.039
0.666TrpAsn: 0.666 ± 0.048
0.78TrpPro: 0.78 ± 0.058
0.798TrpGln: 0.798 ± 0.049
1.29TrpArg: 1.29 ± 0.065
1.03TrpSer: 1.03 ± 0.059
1.123TrpThr: 1.123 ± 0.059
1.009TrpVal: 1.009 ± 0.05
0.419TrpTrp: 0.419 ± 0.035
0.328TrpTyr: 0.328 ± 0.03
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.294TyrAla: 2.294 ± 0.081
0.283TyrCys: 0.283 ± 0.027
1.402TyrAsp: 1.402 ± 0.069
1.449TyrGlu: 1.449 ± 0.061
1.123TyrPhe: 1.123 ± 0.043
2.093TyrGly: 2.093 ± 0.064
0.588TyrHis: 0.588 ± 0.037
0.801TyrIle: 0.801 ± 0.045
0.798TyrLys: 0.798 ± 0.042
2.252TyrLeu: 2.252 ± 0.079
0.445TyrMet: 0.445 ± 0.03
0.77TyrAsn: 0.77 ± 0.056
1.199TyrPro: 1.199 ± 0.053
0.91TyrGln: 0.91 ± 0.053
1.722TyrArg: 1.722 ± 0.064
1.389TyrSer: 1.389 ± 0.062
1.347TyrThr: 1.347 ± 0.067
1.75TyrVal: 1.75 ± 0.073
0.434TyrTrp: 0.434 ± 0.036
0.804TyrTyr: 0.804 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1135 proteins (384530 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski