Mesorhizobium sp. YR577

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7D715|A0A1I7D715_9RHIZ Crotonobetainyl-CoA hydratase OS=Mesorhizobium sp. YR577 OX=1884373 GN=SAMN05518861_111218 PE=3 SV=1
MM1 pKa = 6.86NTVLNAKK8 pKa = 8.71GVPLPYY14 pKa = 10.09SGSSVNHH21 pKa = 6.18FSATNSGPQLAGSALNDD38 pKa = 3.96SMWGDD43 pKa = 3.41SSVNVVMQGGTGDD56 pKa = 4.42DD57 pKa = 2.84IYY59 pKa = 11.62YY60 pKa = 10.35LYY62 pKa = 10.59SARR65 pKa = 11.84NSAFEE70 pKa = 3.96KK71 pKa = 10.56AGEE74 pKa = 4.29GVDD77 pKa = 5.09TINTWMSYY85 pKa = 10.37RR86 pKa = 11.84LPEE89 pKa = 4.5NIEE92 pKa = 3.93NLTVTGNGRR101 pKa = 11.84SAIGNDD107 pKa = 3.26GDD109 pKa = 4.73NILSGASGSQTIDD122 pKa = 2.68GGAGDD127 pKa = 4.95DD128 pKa = 3.76VLIGGGGADD137 pKa = 2.8IFVISEE143 pKa = 4.3GNGSDD148 pKa = 4.84LILDD152 pKa = 4.16FSVDD156 pKa = 3.47DD157 pKa = 3.73QVRR160 pKa = 11.84LDD162 pKa = 3.67GYY164 pKa = 11.11GFISFDD170 pKa = 4.09AIQSNMTQTGANVTLDD186 pKa = 3.79LGNDD190 pKa = 3.81EE191 pKa = 4.85ILVFANTTVDD201 pKa = 3.77QFDD204 pKa = 3.49AGQFKK209 pKa = 11.03LSLDD213 pKa = 3.54KK214 pKa = 11.58SEE216 pKa = 4.57MSLSFSDD223 pKa = 4.84DD224 pKa = 3.76FNSLSLWNGEE234 pKa = 4.05SGTWDD239 pKa = 3.43SNFWWGAEE247 pKa = 3.87NGSTHH252 pKa = 6.55EE253 pKa = 4.64GNGEE257 pKa = 4.01KK258 pKa = 9.65QWYY261 pKa = 9.12IDD263 pKa = 3.29TDD265 pKa = 3.88YY266 pKa = 11.83APTKK270 pKa = 10.1SVNPFSVDD278 pKa = 3.1NGVLTITAAPTPDD291 pKa = 4.15AIKK294 pKa = 10.72PEE296 pKa = 4.19VNNYY300 pKa = 10.76DD301 pKa = 3.71YY302 pKa = 11.4TSGLLTTYY310 pKa = 10.65EE311 pKa = 4.7SFSQTYY317 pKa = 10.09GYY319 pKa = 11.18FEE321 pKa = 4.44MRR323 pKa = 11.84ADD325 pKa = 3.44MPEE328 pKa = 3.97NQGVWPAFWLLPADD342 pKa = 4.39GSWPPEE348 pKa = 3.76LDD350 pKa = 3.52VVEE353 pKa = 4.68MRR355 pKa = 11.84GQEE358 pKa = 3.94PGVVHH363 pKa = 5.48VTSHH367 pKa = 5.1TNEE370 pKa = 4.46TGSHH374 pKa = 5.33TSVSSAVNVPDD385 pKa = 3.19TSGFHH390 pKa = 6.35TYY392 pKa = 9.8GVLWTEE398 pKa = 4.54EE399 pKa = 4.13EE400 pKa = 4.36IVWYY404 pKa = 10.3FDD406 pKa = 3.31DD407 pKa = 4.97VEE409 pKa = 4.25IARR412 pKa = 11.84TDD414 pKa = 3.66TPSDD418 pKa = 3.26MHH420 pKa = 8.21GPMYY424 pKa = 10.15MLVNLAVGGVAGTPADD440 pKa = 3.9GLATPAEE447 pKa = 4.02MQIDD451 pKa = 4.18YY452 pKa = 10.81IKK454 pKa = 10.54AYY456 pKa = 9.55EE457 pKa = 4.09LDD459 pKa = 3.71GVTQAAAKK467 pKa = 10.15AGSGDD472 pKa = 3.65FLVV475 pKa = 4.16

Molecular weight: 50.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6ZVZ7|A0A1I6ZVZ7_9RHIZ Manganese/iron transport system permease protein OS=Mesorhizobium sp. YR577 OX=1884373 GN=SAMN05518861_103255 PE=3 SV=1
MM1 pKa = 8.09DD2 pKa = 4.95KK3 pKa = 11.06GDD5 pKa = 3.98RR6 pKa = 11.84RR7 pKa = 11.84PLASRR12 pKa = 11.84NTRR15 pKa = 11.84WAQTIAKK22 pKa = 9.45RR23 pKa = 11.84MAALSITPNAISQASIAAAAVAGLAFWIAGEE54 pKa = 4.17TSGTARR60 pKa = 11.84SLLLVAAALFCQLRR74 pKa = 11.84LLCNLFDD81 pKa = 3.98GMVAVEE87 pKa = 4.26GGKK90 pKa = 9.78AAADD94 pKa = 3.84GPFWNEE100 pKa = 3.4FPDD103 pKa = 4.88RR104 pKa = 11.84IADD107 pKa = 3.62ILILAGVGYY116 pKa = 10.69GIGAPSLGWAAAAFAVLTAYY136 pKa = 10.13VRR138 pKa = 11.84EE139 pKa = 4.14LGRR142 pKa = 11.84ANGAPSDD149 pKa = 4.12FSGPMAKK156 pKa = 7.72QHH158 pKa = 5.49RR159 pKa = 11.84MAVITAAALFSAVEE173 pKa = 4.12FLWHH177 pKa = 6.23GRR179 pKa = 11.84NEE181 pKa = 4.25VLTLALSIVAIGASLTAFRR200 pKa = 11.84RR201 pKa = 11.84ARR203 pKa = 11.84RR204 pKa = 11.84QVRR207 pKa = 11.84WLKK210 pKa = 9.88EE211 pKa = 3.66RR212 pKa = 11.84KK213 pKa = 8.84AWQSTPP219 pKa = 3.1

Molecular weight: 23.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6444

0

6444

2014053

26

7420

312.5

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.005 ± 0.04

0.786 ± 0.009

5.633 ± 0.031

5.705 ± 0.032

3.951 ± 0.022

8.592 ± 0.039

1.953 ± 0.016

5.633 ± 0.027

3.742 ± 0.026

9.868 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.56 ± 0.017

2.835 ± 0.025

4.976 ± 0.022

2.988 ± 0.016

6.561 ± 0.036

5.725 ± 0.025

5.421 ± 0.046

7.466 ± 0.025

1.314 ± 0.012

2.286 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski