Phaeobacter inhibens (strain ATCC 700781 / DSM 17395 / CIP 105210 / JCM 21319 / NBRC 16654 / NCIMB 13546 / BS107)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Phaeobacter; Phaeobacter inhibens

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7EX70|I7EX70_PHAIB Glutamate racemase OS=Phaeobacter inhibens (strain ATCC 700781 / DSM 17395 / CIP 105210 / JCM 21319 / NBRC 16654 / NCIMB 13546 / BS107) OX=391619 GN=murI PE=3 SV=1
MM1 pKa = 6.9TRR3 pKa = 11.84IVSMAAIAAALSTPAFAGNLEE24 pKa = 4.27EE25 pKa = 4.73PVVAPAPTAPPVVVANDD42 pKa = 3.5GGDD45 pKa = 2.96WTGAYY50 pKa = 9.36IGGQIGQLDD59 pKa = 4.37ADD61 pKa = 4.19TSNGLSGDD69 pKa = 3.68DD70 pKa = 3.35VSYY73 pKa = 10.89GVHH76 pKa = 6.6AGYY79 pKa = 10.81NYY81 pKa = 10.74DD82 pKa = 3.24FGRR85 pKa = 11.84FVLGGEE91 pKa = 3.92IDD93 pKa = 3.8YY94 pKa = 10.99DD95 pKa = 3.76ATDD98 pKa = 3.11VDD100 pKa = 4.8LGGGAATVDD109 pKa = 3.15SVMRR113 pKa = 11.84GKK115 pKa = 10.59VKK117 pKa = 10.52AGYY120 pKa = 10.48DD121 pKa = 3.75FGPVLAYY128 pKa = 9.06VTGGVAQVDD137 pKa = 3.83TSVGDD142 pKa = 3.67EE143 pKa = 3.88TGEE146 pKa = 3.95FYY148 pKa = 11.06GIGVAYY154 pKa = 10.01RR155 pKa = 11.84VTDD158 pKa = 3.04QWTVGGEE165 pKa = 4.17VLEE168 pKa = 4.84HH169 pKa = 7.18DD170 pKa = 4.56FDD172 pKa = 4.5NLGSSGISADD182 pKa = 3.18ATTVSLRR189 pKa = 11.84ASYY192 pKa = 10.7QFF194 pKa = 3.7

Molecular weight: 19.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7EIP2|I7EIP2_PHAIB Putative HTH-type transcriptional regulator OS=Phaeobacter inhibens (strain ATCC 700781 / DSM 17395 / CIP 105210 / JCM 21319 / NBRC 16654 / NCIMB 13546 / BS107) OX=391619 GN=PGA1_c02700 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.55ILNSRR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3853

0

3853

1234078

35

2162

320.3

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.125 ± 0.055

0.938 ± 0.013

6.156 ± 0.034

5.809 ± 0.032

3.591 ± 0.027

8.49 ± 0.039

2.133 ± 0.02

5.084 ± 0.028

3.185 ± 0.032

10.2 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.02

2.685 ± 0.021

4.999 ± 0.025

3.632 ± 0.024

6.547 ± 0.042

5.422 ± 0.026

5.518 ± 0.027

7.047 ± 0.036

1.38 ± 0.018

2.268 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski