Amino acid dipepetide frequency for Streptococcus pneumoniae SP14-BS69

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.046AlaAla: 5.046 ± 0.124	0.542AlaCys: 0.542 ± 0.03	4.004AlaAsp: 4.004 ± 0.082	5.124AlaGlu: 5.124 ± 0.103	3.31AlaPhe: 3.31 ± 0.084	5.364AlaGly: 5.364 ± 0.104	1.361AlaHis: 1.361 ± 0.049	5.481AlaIle: 5.481 ± 0.109	4.981AlaLys: 4.981 ± 0.11	7.211AlaLeu: 7.211 ± 0.119	1.87AlaMet: 1.87 ± 0.062	2.921AlaAsn: 2.921 ± 0.078	2.156AlaPro: 2.156 ± 0.072	2.983AlaGln: 2.983 ± 0.072	2.866AlaArg: 2.866 ± 0.07	4.422AlaSer: 4.422 ± 0.088	4.074AlaThr: 4.074 ± 0.099	5.055AlaVal: 5.055 ± 0.102	0.631AlaTrp: 0.631 ± 0.037	2.711AlaTyr: 2.711 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.304CysAla: 0.304 ± 0.023	0.08CysCys: 0.08 ± 0.013	0.346CysAsp: 0.346 ± 0.024	0.311CysGlu: 0.311 ± 0.025	0.387CysPhe: 0.387 ± 0.033	0.564CysGly: 0.564 ± 0.031	0.203CysHis: 0.203 ± 0.018	0.374CysIle: 0.374 ± 0.024	0.374CysLys: 0.374 ± 0.026	0.722CysLeu: 0.722 ± 0.039	0.171CysMet: 0.171 ± 0.016	0.229CysAsn: 0.229 ± 0.018	0.249CysPro: 0.249 ± 0.019	0.338CysGln: 0.338 ± 0.022	0.317CysArg: 0.317 ± 0.023	0.485CysSer: 0.485 ± 0.029	0.231CysThr: 0.231 ± 0.019	0.381CysVal: 0.381 ± 0.024	0.088CysTrp: 0.088 ± 0.011	0.276CysTyr: 0.276 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.553AspAla: 3.553 ± 0.078	0.329AspCys: 0.329 ± 0.021	2.548AspAsp: 2.548 ± 0.071	4.05AspGlu: 4.05 ± 0.099	3.313AspPhe: 3.313 ± 0.07	3.915AspGly: 3.915 ± 0.163	0.974AspHis: 0.974 ± 0.04	4.004AspIle: 4.004 ± 0.083	4.412AspLys: 4.412 ± 0.092	5.998AspLeu: 5.998 ± 0.108	1.48AspMet: 1.48 ± 0.047	2.321AspAsn: 2.321 ± 0.065	1.519AspPro: 1.519 ± 0.052	2.069AspGln: 2.069 ± 0.058	2.132AspArg: 2.132 ± 0.062	3.077AspSer: 3.077 ± 0.081	2.521AspThr: 2.521 ± 0.069	3.599AspVal: 3.599 ± 0.07	0.649AspTrp: 0.649 ± 0.034	2.753AspTyr: 2.753 ± 0.076	0.002AspXaa: 0.002 ± 0.002
Glu	5.422GluAla: 5.422 ± 0.107	0.338GluCys: 0.338 ± 0.029	3.745GluAsp: 3.745 ± 0.084	6.53GluGlu: 6.53 ± 0.129	2.727GluPhe: 2.727 ± 0.065	3.771GluGly: 3.771 ± 0.076	1.291GluHis: 1.291 ± 0.045	5.679GluIle: 5.679 ± 0.1	6.57GluLys: 6.57 ± 0.134	7.213GluLeu: 7.213 ± 0.112	1.914GluMet: 1.914 ± 0.05	4.051GluAsn: 4.051 ± 0.081	1.618GluPro: 1.618 ± 0.057	2.844GluGln: 2.844 ± 0.076	3.42GluArg: 3.42 ± 0.094	3.643GluSer: 3.643 ± 0.08	3.885GluThr: 3.885 ± 0.081	5.315GluVal: 5.315 ± 0.121	0.686GluTrp: 0.686 ± 0.044	2.127GluTyr: 2.127 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	3.305PheAla: 3.305 ± 0.081	0.332PheCys: 0.332 ± 0.022	2.911PheAsp: 2.911 ± 0.065	3.295PheGlu: 3.295 ± 0.095	2.436PhePhe: 2.436 ± 0.081	3.119PheGly: 3.119 ± 0.073	0.865PheHis: 0.865 ± 0.041	3.147PheIle: 3.147 ± 0.082	2.653PheLys: 2.653 ± 0.065	5.003PheLeu: 5.003 ± 0.125	1.138PheMet: 1.138 ± 0.05	1.906PheAsn: 1.906 ± 0.064	1.485PhePro: 1.485 ± 0.049	1.769PheGln: 1.769 ± 0.059	1.597PheArg: 1.597 ± 0.057	3.379PheSer: 3.379 ± 0.083	2.415PheThr: 2.415 ± 0.064	3.21PheVal: 3.21 ± 0.068	0.537PheTrp: 0.537 ± 0.028	2.01PheTyr: 2.01 ± 0.062	0.0PheXaa: 0.0 ± 0.0
Gly	4.565GlyAla: 4.565 ± 0.102	0.46GlyCys: 0.46 ± 0.028	3.327GlyAsp: 3.327 ± 0.076	3.903GlyGlu: 3.903 ± 0.09	3.217GlyPhe: 3.217 ± 0.07	4.09GlyGly: 4.09 ± 0.093	1.29GlyHis: 1.29 ± 0.051	5.22GlyIle: 5.22 ± 0.098	4.889GlyLys: 4.889 ± 0.124	6.645GlyLeu: 6.645 ± 0.116	1.74GlyMet: 1.74 ± 0.056	2.758GlyAsn: 2.758 ± 0.071	1.298GlyPro: 1.298 ± 0.048	3.035GlyGln: 3.035 ± 0.074	2.77GlyArg: 2.77 ± 0.086	3.677GlySer: 3.677 ± 0.076	3.61GlyThr: 3.61 ± 0.089	4.915GlyVal: 4.915 ± 0.099	0.742GlyTrp: 0.742 ± 0.043	2.584GlyTyr: 2.584 ± 0.079	0.0GlyXaa: 0.0 ± 0.0
His	1.155HisAla: 1.155 ± 0.04	0.223HisCys: 0.223 ± 0.026	0.947HisAsp: 0.947 ± 0.037	1.124HisGlu: 1.124 ± 0.047	1.174HisPhe: 1.174 ± 0.049	1.264HisGly: 1.264 ± 0.046	0.59HisHis: 0.59 ± 0.03	1.325HisIle: 1.325 ± 0.047	1.02HisLys: 1.02 ± 0.041	2.098HisLeu: 2.098 ± 0.062	0.452HisMet: 0.452 ± 0.027	0.729HisAsn: 0.729 ± 0.031	0.934HisPro: 0.934 ± 0.041	0.958HisGln: 0.958 ± 0.04	0.925HisArg: 0.925 ± 0.035	1.119HisSer: 1.119 ± 0.046	0.934HisThr: 0.934 ± 0.044	1.174HisVal: 1.174 ± 0.041	0.172HisTrp: 0.172 ± 0.017	1.077HisTyr: 1.077 ± 0.042	0.0HisXaa: 0.0 ± 0.0
Ile	5.76IleAla: 5.76 ± 0.099	0.566IleCys: 0.566 ± 0.031	4.206IleAsp: 4.206 ± 0.086	5.095IleGlu: 5.095 ± 0.094	3.501IlePhe: 3.501 ± 0.088	4.78IleGly: 4.78 ± 0.097	1.373IleHis: 1.373 ± 0.052	4.944IleIle: 4.944 ± 0.115	4.554IleLys: 4.554 ± 0.096	7.632IleLeu: 7.632 ± 0.129	1.579IleMet: 1.579 ± 0.053	2.952IleAsn: 2.952 ± 0.08	2.765IlePro: 2.765 ± 0.067	2.807IleGln: 2.807 ± 0.07	3.01IleArg: 3.01 ± 0.069	5.053IleSer: 5.053 ± 0.098	3.697IleThr: 3.697 ± 0.077	4.837IleVal: 4.837 ± 0.108	0.654IleTrp: 0.654 ± 0.032	2.568IleTyr: 2.568 ± 0.073	0.002IleXaa: 0.002 ± 0.001
Lys	4.82LysAla: 4.82 ± 0.099	0.281LysCys: 0.281 ± 0.018	4.176LysAsp: 4.176 ± 0.094	6.715LysGlu: 6.715 ± 0.133	2.057LysPhe: 2.057 ± 0.063	4.087LysGly: 4.087 ± 0.09	1.314LysHis: 1.314 ± 0.049	5.081LysIle: 5.081 ± 0.111	5.747LysLys: 5.747 ± 0.138	6.221LysLeu: 6.221 ± 0.11	2.119LysMet: 2.119 ± 0.057	3.555LysAsn: 3.555 ± 0.095	2.135LysPro: 2.135 ± 0.073	2.836LysGln: 2.836 ± 0.07	3.23LysArg: 3.23 ± 0.079	3.937LysSer: 3.937 ± 0.095	3.958LysThr: 3.958 ± 0.089	4.765LysVal: 4.765 ± 0.093	0.68LysTrp: 0.68 ± 0.035	2.335LysTyr: 2.335 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	8.621LeuAla: 8.621 ± 0.153	0.597LeuCys: 0.597 ± 0.031	5.834LeuAsp: 5.834 ± 0.101	7.461LeuGlu: 7.461 ± 0.141	4.63LeuPhe: 4.63 ± 0.126	6.468LeuGly: 6.468 ± 0.106	1.672LeuHis: 1.672 ± 0.052	6.656LeuIle: 6.656 ± 0.117	6.652LeuLys: 6.652 ± 0.117	10.348LeuLeu: 10.348 ± 0.202	2.532LeuMet: 2.532 ± 0.069	4.134LeuAsn: 4.134 ± 0.098	4.017LeuPro: 4.017 ± 0.081	3.549LeuGln: 3.549 ± 0.087	3.812LeuArg: 3.812 ± 0.072	7.46LeuSer: 7.46 ± 0.116	6.164LeuThr: 6.164 ± 0.098	7.086LeuVal: 7.086 ± 0.119	0.75LeuTrp: 0.75 ± 0.04	3.368LeuTyr: 3.368 ± 0.078	0.0LeuXaa: 0.0 ± 0.0
Met	1.908MetAla: 1.908 ± 0.065	0.12MetCys: 0.12 ± 0.015	1.433MetAsp: 1.433 ± 0.055	1.677MetGlu: 1.677 ± 0.051	0.829MetPhe: 0.829 ± 0.041	1.589MetGly: 1.589 ± 0.059	0.361MetHis: 0.361 ± 0.022	1.926MetIle: 1.926 ± 0.059	2.293MetLys: 2.293 ± 0.062	2.288MetLeu: 2.288 ± 0.065	0.825MetMet: 0.825 ± 0.036	1.347MetAsn: 1.347 ± 0.047	0.825MetPro: 0.825 ± 0.037	0.896MetGln: 0.896 ± 0.041	1.051MetArg: 1.051 ± 0.039	1.617MetSer: 1.617 ± 0.05	1.854MetThr: 1.854 ± 0.055	1.682MetVal: 1.682 ± 0.055	0.192MetTrp: 0.192 ± 0.017	0.584MetTyr: 0.584 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	2.786AsnAla: 2.786 ± 0.075	0.314AsnCys: 0.314 ± 0.023	2.127AsnAsp: 2.127 ± 0.059	2.493AsnGlu: 2.493 ± 0.069	1.971AsnPhe: 1.971 ± 0.064	3.365AsnGly: 3.365 ± 0.087	1.151AsnHis: 1.151 ± 0.042	3.165AsnIle: 3.165 ± 0.076	2.817AsnLys: 2.817 ± 0.078	4.754AsnLeu: 4.754 ± 0.096	1.155AsnMet: 1.155 ± 0.046	1.898AsnAsn: 1.898 ± 0.064	2.306AsnPro: 2.306 ± 0.06	2.251AsnGln: 2.251 ± 0.057	2.134AsnArg: 2.134 ± 0.059	2.413AsnSer: 2.413 ± 0.068	2.106AsnThr: 2.106 ± 0.068	2.589AsnVal: 2.589 ± 0.065	0.558AsnTrp: 0.558 ± 0.026	1.893AsnTyr: 1.893 ± 0.056	0.0AsnXaa: 0.0 ± 0.0
Pro	2.43ProAla: 2.43 ± 0.073	0.205ProCys: 0.205 ± 0.018	1.968ProAsp: 1.968 ± 0.053	2.965ProGlu: 2.965 ± 0.071	1.625ProPhe: 1.625 ± 0.047	1.623ProGly: 1.623 ± 0.052	0.673ProHis: 0.673 ± 0.037	2.4ProIle: 2.4 ± 0.057	1.999ProLys: 1.999 ± 0.065	2.927ProLeu: 2.927 ± 0.073	0.768ProMet: 0.768 ± 0.036	1.612ProAsn: 1.612 ± 0.057	0.571ProPro: 0.571 ± 0.031	1.304ProGln: 1.304 ± 0.055	1.116ProArg: 1.116 ± 0.044	2.15ProSer: 2.15 ± 0.074	1.948ProThr: 1.948 ± 0.065	2.667ProVal: 2.667 ± 0.068	0.298ProTrp: 0.298 ± 0.022	1.299ProTyr: 1.299 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	3.601GlnAla: 3.601 ± 0.09	0.135GlnCys: 0.135 ± 0.014	2.207GlnAsp: 2.207 ± 0.067	3.584GlnGlu: 3.584 ± 0.08	1.711GlnPhe: 1.711 ± 0.05	2.57GlnGly: 2.57 ± 0.068	0.704GlnHis: 0.704 ± 0.036	2.965GlnIle: 2.965 ± 0.063	2.95GlnLys: 2.95 ± 0.083	4.121GlnLeu: 4.121 ± 0.09	0.956GlnMet: 0.956 ± 0.036	1.76GlnAsn: 1.76 ± 0.052	1.158GlnPro: 1.158 ± 0.045	1.434GlnGln: 1.434 ± 0.059	1.493GlnArg: 1.493 ± 0.053	2.174GlnSer: 2.174 ± 0.066	2.426GlnThr: 2.426 ± 0.069	3.277GlnVal: 3.277 ± 0.086	0.327GlnTrp: 0.327 ± 0.025	1.314GlnTyr: 1.314 ± 0.052	0.0GlnXaa: 0.0 ± 0.0
Arg	2.537ArgAla: 2.537 ± 0.067	0.239ArgCys: 0.239 ± 0.019	2.267ArgAsp: 2.267 ± 0.066	3.233ArgGlu: 3.233 ± 0.072	2.132ArgPhe: 2.132 ± 0.068	2.334ArgGly: 2.334 ± 0.069	0.803ArgHis: 0.803 ± 0.036	3.105ArgIle: 3.105 ± 0.076	3.152ArgLys: 3.152 ± 0.076	4.207ArgLeu: 4.207 ± 0.094	1.256ArgMet: 1.256 ± 0.047	1.88ArgAsn: 1.88 ± 0.055	1.286ArgPro: 1.286 ± 0.05	1.887ArgGln: 1.887 ± 0.056	1.987ArgArg: 1.987 ± 0.061	2.082ArgSer: 2.082 ± 0.054	2.145ArgThr: 2.145 ± 0.076	2.81ArgVal: 2.81 ± 0.064	0.353ArgTrp: 0.353 ± 0.026	1.815ArgTyr: 1.815 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	3.719SerAla: 3.719 ± 0.084	0.423SerCys: 0.423 ± 0.029	3.287SerAsp: 3.287 ± 0.073	3.658SerGlu: 3.658 ± 0.086	3.15SerPhe: 3.15 ± 0.085	4.209SerGly: 4.209 ± 0.088	1.36SerHis: 1.36 ± 0.051	4.474SerIle: 4.474 ± 0.086	4.29SerLys: 4.29 ± 0.089	6.728SerLeu: 6.728 ± 0.11	1.555SerMet: 1.555 ± 0.049	2.724SerAsn: 2.724 ± 0.073	1.999SerPro: 1.999 ± 0.059	3.123SerGln: 3.123 ± 0.077	2.674SerArg: 2.674 ± 0.076	4.407SerSer: 4.407 ± 0.111	3.116SerThr: 3.116 ± 0.081	4.023SerVal: 4.023 ± 0.081	0.703SerTrp: 0.703 ± 0.033	2.571SerTyr: 2.571 ± 0.066	0.002SerXaa: 0.002 ± 0.002
Thr	3.939ThrAla: 3.939 ± 0.074	0.369ThrCys: 0.369 ± 0.024	3.197ThrAsp: 3.197 ± 0.072	3.462ThrGlu: 3.462 ± 0.078	2.613ThrPhe: 2.613 ± 0.065	4.051ThrGly: 4.051 ± 0.094	1.096ThrHis: 1.096 ± 0.043	4.485ThrIle: 4.485 ± 0.095	3.313ThrLys: 3.313 ± 0.071	5.175ThrLeu: 5.175 ± 0.1	1.073ThrMet: 1.073 ± 0.042	2.212ThrAsn: 2.212 ± 0.061	2.264ThrPro: 2.264 ± 0.078	1.713ThrGln: 1.713 ± 0.055	2.095ThrArg: 2.095 ± 0.065	3.638ThrSer: 3.638 ± 0.084	3.057ThrThr: 3.057 ± 0.086	4.336ThrVal: 4.336 ± 0.096	0.592ThrTrp: 0.592 ± 0.029	2.139ThrTyr: 2.139 ± 0.064	0.0ThrXaa: 0.0 ± 0.0
Val	5.647ValAla: 5.647 ± 0.116	0.504ValCys: 0.504 ± 0.029	3.97ValAsp: 3.97 ± 0.083	5.198ValGlu: 5.198 ± 0.108	3.207ValPhe: 3.207 ± 0.083	4.497ValGly: 4.497 ± 0.088	1.184ValHis: 1.184 ± 0.04	4.778ValIle: 4.778 ± 0.083	4.489ValLys: 4.489 ± 0.094	7.013ValLeu: 7.013 ± 0.124	1.586ValMet: 1.586 ± 0.048	3.092ValAsn: 3.092 ± 0.066	2.394ValPro: 2.394 ± 0.063	2.322ValGln: 2.322 ± 0.058	2.683ValArg: 2.683 ± 0.066	4.485ValSer: 4.485 ± 0.086	4.298ValThr: 4.298 ± 0.09	4.898ValVal: 4.898 ± 0.104	0.566ValTrp: 0.566 ± 0.034	2.446ValTyr: 2.446 ± 0.061	0.0ValXaa: 0.0 ± 0.0
Trp	0.543TrpAla: 0.543 ± 0.032	0.067TrpCys: 0.067 ± 0.01	0.517TrpAsp: 0.517 ± 0.03	0.615TrpGlu: 0.615 ± 0.035	0.529TrpPhe: 0.529 ± 0.032	0.625TrpGly: 0.625 ± 0.031	0.197TrpHis: 0.197 ± 0.021	0.714TrpIle: 0.714 ± 0.033	0.681TrpLys: 0.681 ± 0.033	1.041TrpLeu: 1.041 ± 0.047	0.273TrpMet: 0.273 ± 0.021	0.561TrpAsn: 0.561 ± 0.03	0.172TrpPro: 0.172 ± 0.017	0.434TrpGln: 0.434 ± 0.03	0.395TrpArg: 0.395 ± 0.028	0.598TrpSer: 0.598 ± 0.033	0.55TrpThr: 0.55 ± 0.034	0.53TrpVal: 0.53 ± 0.032	0.143TrpTrp: 0.143 ± 0.019	0.507TrpTyr: 0.507 ± 0.044	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.451TyrAla: 2.451 ± 0.063	0.307TyrCys: 0.307 ± 0.023	2.199TyrAsp: 2.199 ± 0.071	2.3TyrGlu: 2.3 ± 0.063	1.992TyrPhe: 1.992 ± 0.067	2.452TyrGly: 2.452 ± 0.064	0.927TyrHis: 0.927 ± 0.042	2.41TyrIle: 2.41 ± 0.067	2.217TyrLys: 2.217 ± 0.067	4.466TyrLeu: 4.466 ± 0.101	0.851TyrMet: 0.851 ± 0.04	1.605TyrAsn: 1.605 ± 0.059	1.397TyrPro: 1.397 ± 0.052	2.314TyrGln: 2.314 ± 0.065	1.804TyrArg: 1.804 ± 0.058	2.376TyrSer: 2.376 ± 0.07	1.83TyrThr: 1.83 ± 0.058	2.106TyrVal: 2.106 ± 0.058	0.374TyrTrp: 0.374 ± 0.022	1.804TyrTyr: 1.804 ± 0.074	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.002XaaHis: 0.002 ± 0.002	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.002XaaGln: 0.002 ± 0.002	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2767 proteins (614891 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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