Actinobacillus equuli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Actinobacillus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4840 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A448QV66|A0A448QV66_ACTEU Branched-chain amino acid aminotransferase OS=Actinobacillus equuli OX=718 GN=ilvE_1 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.08KK3 pKa = 10.51CLYY6 pKa = 9.98EE7 pKa = 4.09YY8 pKa = 10.43IAAFEE13 pKa = 4.57FDD15 pKa = 5.26DD16 pKa = 5.34EE17 pKa = 4.34PTKK20 pKa = 11.36DD21 pKa = 3.9DD22 pKa = 3.43VLKK25 pKa = 10.69IISEE29 pKa = 4.34GSWDD33 pKa = 3.7LFKK36 pKa = 10.83CQKK39 pKa = 9.53MLLGYY44 pKa = 9.04VAQEE48 pKa = 3.84ILEE51 pKa = 4.26EE52 pKa = 4.92KK53 pKa = 10.07YY54 pKa = 11.05SDD56 pKa = 2.78WEE58 pKa = 4.24MYY60 pKa = 10.8DD61 pKa = 3.45EE62 pKa = 5.44DD63 pKa = 4.32EE64 pKa = 4.3SVYY67 pKa = 10.94LIVRR71 pKa = 11.84KK72 pKa = 10.47YY73 pKa = 10.91GDD75 pKa = 4.22EE76 pKa = 3.63EE77 pKa = 4.6WEE79 pKa = 4.04LHH81 pKa = 6.05KK82 pKa = 10.82ATIQYY87 pKa = 10.46RR88 pKa = 11.84LYY90 pKa = 10.83VDD92 pKa = 3.29TEE94 pKa = 4.15EE95 pKa = 5.6IYY97 pKa = 10.84FDD99 pKa = 5.04DD100 pKa = 4.82EE101 pKa = 4.17EE102 pKa = 4.27

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S4X346|A0A3S4X346_ACTEU Heavy metal-dependent transcriptional regulator OS=Actinobacillus equuli OX=718 GN=hmrR_1 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.73TVVKK29 pKa = 9.83FF30 pKa = 3.55

Molecular weight: 3.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4840

0

4840

579404

29

835

119.7

13.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.754 ± 0.054

1.095 ± 0.017

4.677 ± 0.032

5.993 ± 0.046

4.21 ± 0.037

6.623 ± 0.04

2.077 ± 0.021

6.698 ± 0.044

5.995 ± 0.038

10.379 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.976 ± 0.024

4.745 ± 0.033

3.75 ± 0.027

4.751 ± 0.044

4.693 ± 0.034

5.961 ± 0.038

5.25 ± 0.04

7.029 ± 0.036

1.189 ± 0.017

3.159 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski