Amino acid dipepetide frequency for Actinobacillus equuli

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.109AlaAla: 7.109 ± 0.139
1.006AlaCys: 1.006 ± 0.047
4.403AlaAsp: 4.403 ± 0.088
6.403AlaGlu: 6.403 ± 0.12
3.473AlaPhe: 3.473 ± 0.084
5.949AlaGly: 5.949 ± 0.101
1.55AlaHis: 1.55 ± 0.052
6.244AlaIle: 6.244 ± 0.115
6.263AlaLys: 6.263 ± 0.112
9.239AlaLeu: 9.239 ± 0.142
2.61AlaMet: 2.61 ± 0.059
4.13AlaAsn: 4.13 ± 0.096
2.629AlaPro: 2.629 ± 0.069
4.166AlaGln: 4.166 ± 0.095
3.507AlaArg: 3.507 ± 0.079
4.477AlaSer: 4.477 ± 0.088
4.467AlaThr: 4.467 ± 0.098
6.47AlaVal: 6.47 ± 0.128
0.827AlaTrp: 0.827 ± 0.034
2.616AlaTyr: 2.616 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.747CysAla: 0.747 ± 0.034
0.212CysCys: 0.212 ± 0.015
0.537CysAsp: 0.537 ± 0.03
0.613CysGlu: 0.613 ± 0.038
0.449CysPhe: 0.449 ± 0.029
0.894CysGly: 0.894 ± 0.036
0.292CysHis: 0.292 ± 0.023
0.63CysIle: 0.63 ± 0.03
0.545CysLys: 0.545 ± 0.034
1.137CysLeu: 1.137 ± 0.049
0.414CysMet: 0.414 ± 0.026
0.418CysAsn: 0.418 ± 0.03
0.402CysPro: 0.402 ± 0.023
0.421CysGln: 0.421 ± 0.033
0.709CysArg: 0.709 ± 0.036
0.702CysSer: 0.702 ± 0.038
0.476CysThr: 0.476 ± 0.028
0.751CysVal: 0.751 ± 0.036
0.133CysTrp: 0.133 ± 0.014
0.464CysTyr: 0.464 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
3.466AspAla: 3.466 ± 0.076
0.487AspCys: 0.487 ± 0.028
2.202AspAsp: 2.202 ± 0.069
3.41AspGlu: 3.41 ± 0.083
2.349AspPhe: 2.349 ± 0.07
3.26AspGly: 3.26 ± 0.09
0.773AspHis: 0.773 ± 0.039
3.34AspIle: 3.34 ± 0.078
3.058AspLys: 3.058 ± 0.082
4.902AspLeu: 4.902 ± 0.102
1.046AspMet: 1.046 ± 0.036
2.183AspAsn: 2.183 ± 0.072
1.897AspPro: 1.897 ± 0.061
1.716AspGln: 1.716 ± 0.056
2.1AspArg: 2.1 ± 0.068
2.52AspSer: 2.52 ± 0.062
2.078AspThr: 2.078 ± 0.062
3.34AspVal: 3.34 ± 0.082
0.706AspTrp: 0.706 ± 0.035
1.938AspTyr: 1.938 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
5.071GluAla: 5.071 ± 0.102
0.492GluCys: 0.492 ± 0.032
2.456GluAsp: 2.456 ± 0.062
3.573GluGlu: 3.573 ± 0.085
2.278GluPhe: 2.278 ± 0.07
3.412GluGly: 3.412 ± 0.075
1.251GluHis: 1.251 ± 0.047
4.346GluIle: 4.346 ± 0.089
4.106GluLys: 4.106 ± 0.093
6.179GluLeu: 6.179 ± 0.118
1.74GluMet: 1.74 ± 0.053
3.267GluAsn: 3.267 ± 0.072
1.864GluPro: 1.864 ± 0.066
4.066GluGln: 4.066 ± 0.101
3.205GluArg: 3.205 ± 0.08
2.975GluSer: 2.975 ± 0.077
3.077GluThr: 3.077 ± 0.084
4.025GluVal: 4.025 ± 0.093
0.784GluTrp: 0.784 ± 0.04
1.759GluTyr: 1.759 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.857PheAla: 3.857 ± 0.082
0.592PheCys: 0.592 ± 0.034
2.409PheAsp: 2.409 ± 0.069
2.515PheGlu: 2.515 ± 0.07
1.5PhePhe: 1.5 ± 0.054
3.371PheGly: 3.371 ± 0.083
0.839PheHis: 0.839 ± 0.039
2.953PheIle: 2.953 ± 0.083
2.137PheLys: 2.137 ± 0.057
3.507PheLeu: 3.507 ± 0.092
1.367PheMet: 1.367 ± 0.052
2.04PheAsn: 2.04 ± 0.054
1.5PhePro: 1.5 ± 0.052
1.408PheGln: 1.408 ± 0.051
1.683PheArg: 1.683 ± 0.056
3.174PheSer: 3.174 ± 0.082
2.289PheThr: 2.289 ± 0.057
2.941PheVal: 2.941 ± 0.081
0.482PheTrp: 0.482 ± 0.026
1.534PheTyr: 1.534 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
5.19GlyAla: 5.19 ± 0.116
0.822GlyCys: 0.822 ± 0.04
2.982GlyAsp: 2.982 ± 0.069
4.247GlyGlu: 4.247 ± 0.091
3.129GlyPhe: 3.129 ± 0.07
4.712GlyGly: 4.712 ± 0.106
1.239GlyHis: 1.239 ± 0.048
4.978GlyIle: 4.978 ± 0.098
4.864GlyLys: 4.864 ± 0.104
6.886GlyLeu: 6.886 ± 0.102
1.988GlyMet: 1.988 ± 0.057
3.022GlyAsn: 3.022 ± 0.069
1.055GlyPro: 1.055 ± 0.044
2.428GlyGln: 2.428 ± 0.06
2.886GlyArg: 2.886 ± 0.067
3.845GlySer: 3.845 ± 0.082
3.448GlyThr: 3.448 ± 0.078
5.147GlyVal: 5.147 ± 0.109
0.901GlyTrp: 0.901 ± 0.039
2.461GlyTyr: 2.461 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
1.338HisAla: 1.338 ± 0.048
0.347HisCys: 0.347 ± 0.024
0.86HisAsp: 0.86 ± 0.04
0.854HisGlu: 0.854 ± 0.039
1.182HisPhe: 1.182 ± 0.039
1.289HisGly: 1.289 ± 0.048
0.623HisHis: 0.623 ± 0.033
1.429HisIle: 1.429 ± 0.046
1.12HisLys: 1.12 ± 0.049
2.325HisLeu: 2.325 ± 0.067
0.685HisMet: 0.685 ± 0.036
0.973HisAsn: 0.973 ± 0.039
0.96HisPro: 0.96 ± 0.042
1.125HisGln: 1.125 ± 0.046
1.082HisArg: 1.082 ± 0.041
1.35HisSer: 1.35 ± 0.048
1.042HisThr: 1.042 ± 0.038
0.941HisVal: 0.941 ± 0.044
0.337HisTrp: 0.337 ± 0.026
0.904HisTyr: 0.904 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
6.572IleAla: 6.572 ± 0.107
0.929IleCys: 0.929 ± 0.042
3.505IleAsp: 3.505 ± 0.075
4.294IleGlu: 4.294 ± 0.091
2.275IlePhe: 2.275 ± 0.058
4.997IleGly: 4.997 ± 0.116
1.265IleHis: 1.265 ± 0.051
4.228IleIle: 4.228 ± 0.085
3.642IleLys: 3.642 ± 0.073
6.196IleLeu: 6.196 ± 0.094
1.935IleMet: 1.935 ± 0.058
3.0IleAsn: 3.0 ± 0.068
2.601IlePro: 2.601 ± 0.082
2.603IleGln: 2.603 ± 0.065
3.226IleArg: 3.226 ± 0.078
4.886IleSer: 4.886 ± 0.09
3.695IleThr: 3.695 ± 0.081
4.434IleVal: 4.434 ± 0.093
0.699IleTrp: 0.699 ± 0.037
2.002IleTyr: 2.002 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
5.844LysAla: 5.844 ± 0.101
0.59LysCys: 0.59 ± 0.034
2.761LysAsp: 2.761 ± 0.081
3.189LysGlu: 3.189 ± 0.083
2.24LysPhe: 2.24 ± 0.068
3.795LysGly: 3.795 ± 0.077
1.384LysHis: 1.384 ± 0.046
3.65LysIle: 3.65 ± 0.074
2.929LysLys: 2.929 ± 0.088
6.058LysLeu: 6.058 ± 0.107
2.069LysMet: 2.069 ± 0.062
2.518LysAsn: 2.518 ± 0.07
2.729LysPro: 2.729 ± 0.065
3.331LysGln: 3.331 ± 0.075
3.464LysArg: 3.464 ± 0.073
3.44LysSer: 3.44 ± 0.078
3.303LysThr: 3.303 ± 0.077
4.398LysVal: 4.398 ± 0.091
0.749LysTrp: 0.749 ± 0.033
1.508LysTyr: 1.508 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
10.143LeuAla: 10.143 ± 0.132
1.106LeuCys: 1.106 ± 0.044
5.098LeuAsp: 5.098 ± 0.094
5.528LeuGlu: 5.528 ± 0.105
4.422LeuPhe: 4.422 ± 0.088
6.659LeuGly: 6.659 ± 0.119
1.974LeuHis: 1.974 ± 0.055
6.536LeuIle: 6.536 ± 0.113
6.143LeuLys: 6.143 ± 0.118
10.407LeuLeu: 10.407 ± 0.156
2.913LeuMet: 2.913 ± 0.069
5.178LeuAsn: 5.178 ± 0.103
4.85LeuPro: 4.85 ± 0.1
4.315LeuGln: 4.315 ± 0.096
4.689LeuArg: 4.689 ± 0.092
7.23LeuSer: 7.23 ± 0.125
5.972LeuThr: 5.972 ± 0.097
6.902LeuVal: 6.902 ± 0.117
1.094LeuTrp: 1.094 ± 0.049
2.634LeuTyr: 2.634 ± 0.067
0.0LeuXaa: 0.0 ± 0.0
Met
3.027MetAla: 3.027 ± 0.075
0.28MetCys: 0.28 ± 0.021
1.194MetAsp: 1.194 ± 0.051
1.401MetGlu: 1.401 ± 0.047
1.03MetPhe: 1.03 ± 0.046
1.867MetGly: 1.867 ± 0.068
0.587MetHis: 0.587 ± 0.029
1.909MetIle: 1.909 ± 0.067
1.931MetLys: 1.931 ± 0.054
3.25MetLeu: 3.25 ± 0.075
0.972MetMet: 0.972 ± 0.04
1.422MetAsn: 1.422 ± 0.051
1.367MetPro: 1.367 ± 0.049
1.567MetGln: 1.567 ± 0.058
1.46MetArg: 1.46 ± 0.062
1.871MetSer: 1.871 ± 0.058
1.648MetThr: 1.648 ± 0.052
2.064MetVal: 2.064 ± 0.063
0.287MetTrp: 0.287 ± 0.021
0.623MetTyr: 0.623 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
4.071AsnAla: 4.071 ± 0.091
0.447AsnCys: 0.447 ± 0.028
2.069AsnAsp: 2.069 ± 0.071
2.601AsnGlu: 2.601 ± 0.07
1.809AsnPhe: 1.809 ± 0.052
3.474AsnGly: 3.474 ± 0.078
0.984AsnHis: 0.984 ± 0.041
3.117AsnIle: 3.117 ± 0.071
2.66AsnLys: 2.66 ± 0.067
4.945AsnLeu: 4.945 ± 0.09
1.458AsnMet: 1.458 ± 0.049
2.183AsnAsn: 2.183 ± 0.082
2.323AsnPro: 2.323 ± 0.059
2.321AsnGln: 2.321 ± 0.067
2.289AsnArg: 2.289 ± 0.074
2.61AsnSer: 2.61 ± 0.067
2.256AsnThr: 2.256 ± 0.062
3.443AsnVal: 3.443 ± 0.086
0.618AsnTrp: 0.618 ± 0.039
1.766AsnTyr: 1.766 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
2.972ProAla: 2.972 ± 0.064
0.311ProCys: 0.311 ± 0.024
1.659ProAsp: 1.659 ± 0.058
2.943ProGlu: 2.943 ± 0.076
1.854ProPhe: 1.854 ± 0.06
1.307ProGly: 1.307 ± 0.044
0.932ProHis: 0.932 ± 0.037
2.596ProIle: 2.596 ± 0.077
2.273ProLys: 2.273 ± 0.069
3.987ProLeu: 3.987 ± 0.091
1.187ProMet: 1.187 ± 0.045
2.266ProAsn: 2.266 ± 0.063
1.037ProPro: 1.037 ± 0.047
1.79ProGln: 1.79 ± 0.062
1.315ProArg: 1.315 ± 0.05
2.071ProSer: 2.071 ± 0.058
2.363ProThr: 2.363 ± 0.065
2.931ProVal: 2.931 ± 0.081
0.369ProTrp: 0.369 ± 0.026
1.334ProTyr: 1.334 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
4.857GlnAla: 4.857 ± 0.097
0.414GlnCys: 0.414 ± 0.03
1.926GlnAsp: 1.926 ± 0.062
2.299GlnGlu: 2.299 ± 0.06
2.171GlnPhe: 2.171 ± 0.068
2.746GlnGly: 2.746 ± 0.081
1.137GlnHis: 1.137 ± 0.042
3.279GlnIle: 3.279 ± 0.083
2.509GlnLys: 2.509 ± 0.075
5.067GlnLeu: 5.067 ± 0.106
1.25GlnMet: 1.25 ± 0.049
2.273GlnAsn: 2.273 ± 0.062
1.885GlnPro: 1.885 ± 0.062
3.343GlnGln: 3.343 ± 0.101
2.323GlnArg: 2.323 ± 0.07
2.273GlnSer: 2.273 ± 0.064
2.611GlnThr: 2.611 ± 0.072
3.065GlnVal: 3.065 ± 0.076
0.699GlnTrp: 0.699 ± 0.037
1.379GlnTyr: 1.379 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
3.1ArgAla: 3.1 ± 0.069
0.564ArgCys: 0.564 ± 0.033
2.04ArgAsp: 2.04 ± 0.055
2.858ArgGlu: 2.858 ± 0.08
2.468ArgPhe: 2.468 ± 0.073
2.518ArgGly: 2.518 ± 0.063
1.151ArgHis: 1.151 ± 0.041
3.283ArgIle: 3.283 ± 0.08
3.107ArgLys: 3.107 ± 0.078
5.421ArgLeu: 5.421 ± 0.1
1.596ArgMet: 1.596 ± 0.053
2.299ArgAsn: 2.299 ± 0.063
1.66ArgPro: 1.66 ± 0.054
2.304ArgGln: 2.304 ± 0.061
2.375ArgArg: 2.375 ± 0.068
2.629ArgSer: 2.629 ± 0.071
2.213ArgThr: 2.213 ± 0.068
2.894ArgVal: 2.894 ± 0.08
0.58ArgTrp: 0.58 ± 0.033
1.866ArgTyr: 1.866 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
5.302SerAla: 5.302 ± 0.09
0.597SerCys: 0.597 ± 0.03
2.829SerAsp: 2.829 ± 0.076
3.379SerGlu: 3.379 ± 0.088
2.584SerPhe: 2.584 ± 0.063
4.582SerGly: 4.582 ± 0.096
1.4SerHis: 1.4 ± 0.051
3.63SerIle: 3.63 ± 0.087
3.179SerLys: 3.179 ± 0.068
6.293SerLeu: 6.293 ± 0.106
1.698SerMet: 1.698 ± 0.053
2.761SerAsn: 2.761 ± 0.071
2.23SerPro: 2.23 ± 0.061
2.78SerGln: 2.78 ± 0.066
2.622SerArg: 2.622 ± 0.075
3.635SerSer: 3.635 ± 0.085
2.913SerThr: 2.913 ± 0.072
4.531SerVal: 4.531 ± 0.096
0.685SerTrp: 0.685 ± 0.036
1.978SerTyr: 1.978 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
5.021ThrAla: 5.021 ± 0.109
0.409ThrCys: 0.409 ± 0.028
2.61ThrAsp: 2.61 ± 0.069
3.253ThrGlu: 3.253 ± 0.074
2.232ThrPhe: 2.232 ± 0.066
3.87ThrGly: 3.87 ± 0.086
1.08ThrHis: 1.08 ± 0.046
3.474ThrIle: 3.474 ± 0.069
2.787ThrLys: 2.787 ± 0.064
5.718ThrLeu: 5.718 ± 0.099
1.274ThrMet: 1.274 ± 0.053
2.301ThrAsn: 2.301 ± 0.062
2.385ThrPro: 2.385 ± 0.065
2.304ThrGln: 2.304 ± 0.064
2.106ThrArg: 2.106 ± 0.06
2.891ThrSer: 2.891 ± 0.073
2.881ThrThr: 2.881 ± 0.081
4.02ThrVal: 4.02 ± 0.087
0.475ThrTrp: 0.475 ± 0.026
1.407ThrTyr: 1.407 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
6.439ValAla: 6.439 ± 0.112
0.751ValCys: 0.751 ± 0.033
3.226ValAsp: 3.226 ± 0.074
4.537ValGlu: 4.537 ± 0.087
2.646ValPhe: 2.646 ± 0.074
4.619ValGly: 4.619 ± 0.081
1.229ValHis: 1.229 ± 0.042
4.953ValIle: 4.953 ± 0.104
4.505ValLys: 4.505 ± 0.098
7.042ValLeu: 7.042 ± 0.124
2.325ValMet: 2.325 ± 0.064
3.352ValAsn: 3.352 ± 0.095
2.606ValPro: 2.606 ± 0.077
2.554ValGln: 2.554 ± 0.066
3.284ValArg: 3.284 ± 0.078
4.474ValSer: 4.474 ± 0.091
3.895ValThr: 3.895 ± 0.1
5.223ValVal: 5.223 ± 0.086
0.761ValTrp: 0.761 ± 0.04
1.867ValTyr: 1.867 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.906TrpAla: 0.906 ± 0.037
0.173TrpCys: 0.173 ± 0.015
0.545TrpAsp: 0.545 ± 0.034
0.509TrpGlu: 0.509 ± 0.028
0.559TrpPhe: 0.559 ± 0.03
0.668TrpGly: 0.668 ± 0.04
0.305TrpHis: 0.305 ± 0.024
0.73TrpIle: 0.73 ± 0.036
0.72TrpLys: 0.72 ± 0.035
1.641TrpLeu: 1.641 ± 0.054
0.352TrpMet: 0.352 ± 0.028
0.583TrpAsn: 0.583 ± 0.029
0.121TrpPro: 0.121 ± 0.016
1.063TrpGln: 1.063 ± 0.045
0.677TrpArg: 0.677 ± 0.035
0.525TrpSer: 0.525 ± 0.03
0.492TrpThr: 0.492 ± 0.029
0.778TrpVal: 0.778 ± 0.035
0.179TrpTrp: 0.179 ± 0.019
0.361TrpTyr: 0.361 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.506TyrAla: 2.506 ± 0.057
0.419TyrCys: 0.419 ± 0.028
1.458TyrAsp: 1.458 ± 0.052
1.517TyrGlu: 1.517 ± 0.049
1.448TyrPhe: 1.448 ± 0.05
2.242TyrGly: 2.242 ± 0.064
0.77TyrHis: 0.77 ± 0.041
1.678TyrIle: 1.678 ± 0.056
1.474TyrLys: 1.474 ± 0.052
3.593TyrLeu: 3.593 ± 0.08
0.877TyrMet: 0.877 ± 0.037
1.275TyrAsn: 1.275 ± 0.05
1.396TyrPro: 1.396 ± 0.044
1.9TyrGln: 1.9 ± 0.059
1.924TyrArg: 1.924 ± 0.057
2.031TyrSer: 2.031 ± 0.057
1.376TyrThr: 1.376 ± 0.048
1.992TyrVal: 1.992 ± 0.05
0.525TyrTrp: 0.525 ± 0.032
1.186TyrTyr: 1.186 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4840 proteins (579404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski