Amino acid dipepetide frequency for Mycobacterium phage Makemake

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.664AlaAla: 12.664 ± 1.423
1.08AlaCys: 1.08 ± 0.243
6.602AlaAsp: 6.602 ± 0.632
6.422AlaGlu: 6.422 ± 0.683
3.241AlaPhe: 3.241 ± 0.6
7.563AlaGly: 7.563 ± 0.771
1.501AlaHis: 1.501 ± 0.288
4.141AlaIle: 4.141 ± 0.643
4.201AlaLys: 4.201 ± 0.546
8.883AlaLeu: 8.883 ± 0.667
2.041AlaMet: 2.041 ± 0.432
2.641AlaAsn: 2.641 ± 0.34
4.742AlaPro: 4.742 ± 0.63
3.121AlaGln: 3.121 ± 0.49
6.062AlaArg: 6.062 ± 0.661
4.682AlaSer: 4.682 ± 0.632
6.182AlaThr: 6.182 ± 0.662
8.343AlaVal: 8.343 ± 0.779
2.101AlaTrp: 2.101 ± 0.351
2.761AlaTyr: 2.761 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.9CysAla: 0.9 ± 0.261
0.12CysCys: 0.12 ± 0.094
0.6CysAsp: 0.6 ± 0.192
0.72CysGlu: 0.72 ± 0.225
0.18CysPhe: 0.18 ± 0.101
0.72CysGly: 0.72 ± 0.199
0.24CysHis: 0.24 ± 0.116
0.54CysIle: 0.54 ± 0.189
0.3CysLys: 0.3 ± 0.167
0.48CysLeu: 0.48 ± 0.196
0.06CysMet: 0.06 ± 0.05
0.42CysAsn: 0.42 ± 0.137
0.42CysPro: 0.42 ± 0.159
0.18CysGln: 0.18 ± 0.095
0.66CysArg: 0.66 ± 0.199
0.48CysSer: 0.48 ± 0.176
0.3CysThr: 0.3 ± 0.144
0.24CysVal: 0.24 ± 0.1
0.3CysTrp: 0.3 ± 0.136
0.18CysTyr: 0.18 ± 0.146
0.0CysXaa: 0.0 ± 0.0
Asp
6.422AspAla: 6.422 ± 0.65
0.66AspCys: 0.66 ± 0.183
4.922AspAsp: 4.922 ± 0.577
3.541AspGlu: 3.541 ± 0.493
2.281AspPhe: 2.281 ± 0.356
6.362AspGly: 6.362 ± 0.531
1.32AspHis: 1.32 ± 0.316
2.581AspIle: 2.581 ± 0.431
2.521AspLys: 2.521 ± 0.363
6.782AspLeu: 6.782 ± 0.681
1.14AspMet: 1.14 ± 0.225
1.741AspAsn: 1.741 ± 0.307
4.742AspPro: 4.742 ± 0.527
1.621AspGln: 1.621 ± 0.325
4.201AspArg: 4.201 ± 0.473
3.421AspSer: 3.421 ± 0.398
3.961AspThr: 3.961 ± 0.402
4.261AspVal: 4.261 ± 0.54
1.681AspTrp: 1.681 ± 0.363
2.101AspTyr: 2.101 ± 0.355
0.0AspXaa: 0.0 ± 0.0
Glu
6.062GluAla: 6.062 ± 0.679
0.18GluCys: 0.18 ± 0.141
4.742GluAsp: 4.742 ± 0.519
4.622GluGlu: 4.622 ± 0.537
2.161GluPhe: 2.161 ± 0.385
4.442GluGly: 4.442 ± 0.502
1.501GluHis: 1.501 ± 0.316
3.241GluIle: 3.241 ± 0.43
2.341GluLys: 2.341 ± 0.377
6.962GluLeu: 6.962 ± 0.554
1.44GluMet: 1.44 ± 0.273
1.621GluAsn: 1.621 ± 0.342
2.581GluPro: 2.581 ± 0.428
2.761GluGln: 2.761 ± 0.466
3.961GluArg: 3.961 ± 0.575
3.541GluSer: 3.541 ± 0.446
3.721GluThr: 3.721 ± 0.42
5.462GluVal: 5.462 ± 0.578
1.501GluTrp: 1.501 ± 0.351
2.521GluTyr: 2.521 ± 0.483
0.0GluXaa: 0.0 ± 0.0
Phe
2.761PheAla: 2.761 ± 0.385
0.36PheCys: 0.36 ± 0.159
2.941PheAsp: 2.941 ± 0.323
2.221PheGlu: 2.221 ± 0.405
0.6PhePhe: 0.6 ± 0.169
3.781PheGly: 3.781 ± 0.461
0.66PheHis: 0.66 ± 0.212
1.08PheIle: 1.08 ± 0.213
1.561PheLys: 1.561 ± 0.26
2.581PheLeu: 2.581 ± 0.449
0.72PheMet: 0.72 ± 0.224
1.2PheAsn: 1.2 ± 0.216
1.501PhePro: 1.501 ± 0.308
0.96PheGln: 0.96 ± 0.225
1.861PheArg: 1.861 ± 0.35
2.101PheSer: 2.101 ± 0.48
1.921PheThr: 1.921 ± 0.311
2.101PheVal: 2.101 ± 0.391
0.6PheTrp: 0.6 ± 0.179
0.72PheTyr: 0.72 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
7.082GlyAla: 7.082 ± 0.89
0.84GlyCys: 0.84 ± 0.263
6.362GlyAsp: 6.362 ± 0.586
4.622GlyGlu: 4.622 ± 0.56
3.361GlyPhe: 3.361 ± 0.506
10.324GlyGly: 10.324 ± 2.658
1.621GlyHis: 1.621 ± 0.325
4.502GlyIle: 4.502 ± 0.644
3.541GlyLys: 3.541 ± 0.512
7.443GlyLeu: 7.443 ± 1.031
2.221GlyMet: 2.221 ± 0.344
3.541GlyAsn: 3.541 ± 0.514
3.361GlyPro: 3.361 ± 0.523
2.641GlyGln: 2.641 ± 0.289
4.982GlyArg: 4.982 ± 0.472
7.022GlySer: 7.022 ± 1.036
5.282GlyThr: 5.282 ± 0.593
5.462GlyVal: 5.462 ± 0.558
2.401GlyTrp: 2.401 ± 0.334
2.581GlyTyr: 2.581 ± 0.343
0.0GlyXaa: 0.0 ± 0.0
His
1.681HisAla: 1.681 ± 0.373
0.24HisCys: 0.24 ± 0.146
0.96HisAsp: 0.96 ± 0.206
1.501HisGlu: 1.501 ± 0.284
0.78HisPhe: 0.78 ± 0.202
1.861HisGly: 1.861 ± 0.319
0.66HisHis: 0.66 ± 0.201
1.02HisIle: 1.02 ± 0.253
0.96HisLys: 0.96 ± 0.263
1.02HisLeu: 1.02 ± 0.243
0.12HisMet: 0.12 ± 0.084
0.3HisAsn: 0.3 ± 0.118
1.2HisPro: 1.2 ± 0.259
1.02HisGln: 1.02 ± 0.233
1.621HisArg: 1.621 ± 0.333
0.6HisSer: 0.6 ± 0.162
0.9HisThr: 0.9 ± 0.231
1.621HisVal: 1.621 ± 0.312
0.36HisTrp: 0.36 ± 0.144
0.6HisTyr: 0.6 ± 0.207
0.0HisXaa: 0.0 ± 0.0
Ile
6.362IleAla: 6.362 ± 0.734
0.42IleCys: 0.42 ± 0.149
3.481IleAsp: 3.481 ± 0.376
3.421IleGlu: 3.421 ± 0.46
0.84IlePhe: 0.84 ± 0.22
3.661IleGly: 3.661 ± 0.437
0.78IleHis: 0.78 ± 0.191
1.561IleIle: 1.561 ± 0.361
1.741IleLys: 1.741 ± 0.306
3.481IleLeu: 3.481 ± 0.489
0.84IleMet: 0.84 ± 0.197
1.681IleAsn: 1.681 ± 0.268
3.361IlePro: 3.361 ± 0.399
1.621IleGln: 1.621 ± 0.408
3.421IleArg: 3.421 ± 0.439
3.061IleSer: 3.061 ± 0.531
3.001IleThr: 3.001 ± 0.482
3.001IleVal: 3.001 ± 0.577
0.66IleTrp: 0.66 ± 0.166
1.621IleTyr: 1.621 ± 0.268
0.0IleXaa: 0.0 ± 0.0
Lys
3.721LysAla: 3.721 ± 0.521
0.48LysCys: 0.48 ± 0.173
2.461LysAsp: 2.461 ± 0.45
2.161LysGlu: 2.161 ± 0.46
1.621LysPhe: 1.621 ± 0.271
2.341LysGly: 2.341 ± 0.318
1.2LysHis: 1.2 ± 0.312
2.581LysIle: 2.581 ± 0.492
1.801LysLys: 1.801 ± 0.396
3.301LysLeu: 3.301 ± 0.42
1.02LysMet: 1.02 ± 0.22
1.621LysAsn: 1.621 ± 0.255
2.761LysPro: 2.761 ± 0.457
1.44LysGln: 1.44 ± 0.301
2.941LysArg: 2.941 ± 0.489
2.461LysSer: 2.461 ± 0.378
2.401LysThr: 2.401 ± 0.44
3.001LysVal: 3.001 ± 0.467
0.78LysTrp: 0.78 ± 0.219
1.02LysTyr: 1.02 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
9.483LeuAla: 9.483 ± 0.83
0.42LeuCys: 0.42 ± 0.163
5.942LeuAsp: 5.942 ± 0.503
5.762LeuGlu: 5.762 ± 0.551
2.101LeuPhe: 2.101 ± 0.306
7.443LeuGly: 7.443 ± 0.927
1.38LeuHis: 1.38 ± 0.306
4.502LeuIle: 4.502 ± 0.558
4.021LeuLys: 4.021 ± 0.519
5.762LeuLeu: 5.762 ± 0.588
1.681LeuMet: 1.681 ± 0.318
3.301LeuAsn: 3.301 ± 0.403
5.762LeuPro: 5.762 ± 0.607
2.341LeuGln: 2.341 ± 0.37
5.882LeuArg: 5.882 ± 0.559
5.522LeuSer: 5.522 ± 0.639
6.482LeuThr: 6.482 ± 0.497
4.562LeuVal: 4.562 ± 0.65
0.84LeuTrp: 0.84 ± 0.245
2.281LeuTyr: 2.281 ± 0.409
0.0LeuXaa: 0.0 ± 0.0
Met
2.221MetAla: 2.221 ± 0.33
0.0MetCys: 0.0 ± 0.0
0.96MetAsp: 0.96 ± 0.21
1.44MetGlu: 1.44 ± 0.238
0.6MetPhe: 0.6 ± 0.17
1.561MetGly: 1.561 ± 0.299
0.3MetHis: 0.3 ± 0.124
0.72MetIle: 0.72 ± 0.228
1.08MetLys: 1.08 ± 0.263
1.2MetLeu: 1.2 ± 0.231
0.3MetMet: 0.3 ± 0.135
1.08MetAsn: 1.08 ± 0.214
1.08MetPro: 1.08 ± 0.239
0.6MetGln: 0.6 ± 0.147
1.2MetArg: 1.2 ± 0.274
1.921MetSer: 1.921 ± 0.385
2.041MetThr: 2.041 ± 0.289
1.14MetVal: 1.14 ± 0.34
0.24MetTrp: 0.24 ± 0.107
0.48MetTyr: 0.48 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
3.121AsnAla: 3.121 ± 0.497
0.24AsnCys: 0.24 ± 0.146
1.861AsnAsp: 1.861 ± 0.343
1.741AsnGlu: 1.741 ± 0.334
0.84AsnPhe: 0.84 ± 0.213
3.541AsnGly: 3.541 ± 0.552
0.54AsnHis: 0.54 ± 0.144
1.38AsnIle: 1.38 ± 0.338
0.66AsnLys: 0.66 ± 0.199
2.701AsnLeu: 2.701 ± 0.381
0.6AsnMet: 0.6 ± 0.161
0.78AsnAsn: 0.78 ± 0.178
3.181AsnPro: 3.181 ± 0.357
1.02AsnGln: 1.02 ± 0.243
1.26AsnArg: 1.26 ± 0.289
1.981AsnSer: 1.981 ± 0.39
2.161AsnThr: 2.161 ± 0.314
2.401AsnVal: 2.401 ± 0.389
0.66AsnTrp: 0.66 ± 0.175
1.38AsnTyr: 1.38 ± 0.3
0.0AsnXaa: 0.0 ± 0.0
Pro
5.582ProAla: 5.582 ± 0.563
0.48ProCys: 0.48 ± 0.182
4.321ProAsp: 4.321 ± 0.523
4.381ProGlu: 4.381 ± 0.506
2.101ProPhe: 2.101 ± 0.328
5.102ProGly: 5.102 ± 0.656
0.84ProHis: 0.84 ± 0.182
2.341ProIle: 2.341 ± 0.348
2.101ProLys: 2.101 ± 0.292
4.862ProLeu: 4.862 ± 0.489
0.72ProMet: 0.72 ± 0.227
1.561ProAsn: 1.561 ± 0.326
2.581ProPro: 2.581 ± 0.401
1.38ProGln: 1.38 ± 0.302
2.461ProArg: 2.461 ± 0.446
3.841ProSer: 3.841 ± 0.442
4.201ProThr: 4.201 ± 0.538
4.201ProVal: 4.201 ± 0.531
0.72ProTrp: 0.72 ± 0.283
1.501ProTyr: 1.501 ± 0.327
0.0ProXaa: 0.0 ± 0.0
Gln
3.121GlnAla: 3.121 ± 0.513
0.12GlnCys: 0.12 ± 0.088
1.38GlnAsp: 1.38 ± 0.273
1.741GlnGlu: 1.741 ± 0.336
1.32GlnPhe: 1.32 ± 0.269
2.521GlnGly: 2.521 ± 0.369
0.48GlnHis: 0.48 ± 0.131
2.521GlnIle: 2.521 ± 0.476
1.08GlnLys: 1.08 ± 0.235
3.901GlnLeu: 3.901 ± 0.49
0.9GlnMet: 0.9 ± 0.243
0.54GlnAsn: 0.54 ± 0.176
1.921GlnPro: 1.921 ± 0.304
1.741GlnGln: 1.741 ± 0.393
2.281GlnArg: 2.281 ± 0.365
1.561GlnSer: 1.561 ± 0.295
1.741GlnThr: 1.741 ± 0.284
2.461GlnVal: 2.461 ± 0.372
0.6GlnTrp: 0.6 ± 0.176
0.54GlnTyr: 0.54 ± 0.16
0.0GlnXaa: 0.0 ± 0.0
Arg
5.582ArgAla: 5.582 ± 0.684
0.66ArgCys: 0.66 ± 0.182
3.541ArgAsp: 3.541 ± 0.439
4.742ArgGlu: 4.742 ± 0.652
2.041ArgPhe: 2.041 ± 0.37
5.042ArgGly: 5.042 ± 0.628
1.02ArgHis: 1.02 ± 0.254
3.061ArgIle: 3.061 ± 0.45
3.121ArgLys: 3.121 ± 0.49
5.702ArgLeu: 5.702 ± 0.615
1.741ArgMet: 1.741 ± 0.321
2.161ArgAsn: 2.161 ± 0.394
2.221ArgPro: 2.221 ± 0.344
1.861ArgGln: 1.861 ± 0.294
4.502ArgArg: 4.502 ± 0.539
3.721ArgSer: 3.721 ± 0.457
2.941ArgThr: 2.941 ± 0.464
5.162ArgVal: 5.162 ± 0.481
1.44ArgTrp: 1.44 ± 0.351
1.561ArgTyr: 1.561 ± 0.257
0.0ArgXaa: 0.0 ± 0.0
Ser
5.762SerAla: 5.762 ± 0.725
0.42SerCys: 0.42 ± 0.176
3.061SerAsp: 3.061 ± 0.381
3.841SerGlu: 3.841 ± 0.499
2.221SerPhe: 2.221 ± 0.358
7.142SerGly: 7.142 ± 0.95
1.32SerHis: 1.32 ± 0.257
3.061SerIle: 3.061 ± 0.486
2.281SerLys: 2.281 ± 0.325
5.042SerLeu: 5.042 ± 0.652
1.44SerMet: 1.44 ± 0.267
2.221SerAsn: 2.221 ± 0.351
3.421SerPro: 3.421 ± 0.437
2.041SerGln: 2.041 ± 0.319
3.061SerArg: 3.061 ± 0.323
3.661SerSer: 3.661 ± 0.703
3.061SerThr: 3.061 ± 0.454
4.381SerVal: 4.381 ± 0.496
1.32SerTrp: 1.32 ± 0.319
1.32SerTyr: 1.32 ± 0.291
0.0SerXaa: 0.0 ± 0.0
Thr
6.122ThrAla: 6.122 ± 0.717
0.36ThrCys: 0.36 ± 0.163
4.381ThrAsp: 4.381 ± 0.474
4.381ThrGlu: 4.381 ± 0.497
2.161ThrPhe: 2.161 ± 0.39
6.242ThrGly: 6.242 ± 0.567
1.26ThrHis: 1.26 ± 0.277
2.641ThrIle: 2.641 ± 0.474
2.761ThrLys: 2.761 ± 0.36
6.182ThrLeu: 6.182 ± 0.659
0.96ThrMet: 0.96 ± 0.213
1.681ThrAsn: 1.681 ± 0.309
3.841ThrPro: 3.841 ± 0.487
1.801ThrGln: 1.801 ± 0.315
3.301ThrArg: 3.301 ± 0.527
3.361ThrSer: 3.361 ± 0.516
4.502ThrThr: 4.502 ± 0.658
5.282ThrVal: 5.282 ± 0.648
1.08ThrTrp: 1.08 ± 0.216
1.861ThrTyr: 1.861 ± 0.332
0.0ThrXaa: 0.0 ± 0.0
Val
6.482ValAla: 6.482 ± 0.706
0.48ValCys: 0.48 ± 0.171
5.162ValAsp: 5.162 ± 0.652
4.862ValGlu: 4.862 ± 0.534
2.221ValPhe: 2.221 ± 0.322
5.342ValGly: 5.342 ± 0.784
1.26ValHis: 1.26 ± 0.235
3.421ValIle: 3.421 ± 0.405
3.361ValLys: 3.361 ± 0.464
5.462ValLeu: 5.462 ± 0.646
1.14ValMet: 1.14 ± 0.273
2.521ValAsn: 2.521 ± 0.385
4.261ValPro: 4.261 ± 0.536
2.341ValGln: 2.341 ± 0.41
4.802ValArg: 4.802 ± 0.542
4.802ValSer: 4.802 ± 0.417
5.942ValThr: 5.942 ± 0.508
5.462ValVal: 5.462 ± 0.639
1.26ValTrp: 1.26 ± 0.229
2.101ValTyr: 2.101 ± 0.374
0.0ValXaa: 0.0 ± 0.0
Trp
1.621TrpAla: 1.621 ± 0.313
0.12TrpCys: 0.12 ± 0.084
1.32TrpAsp: 1.32 ± 0.26
1.2TrpGlu: 1.2 ± 0.244
0.96TrpPhe: 0.96 ± 0.247
1.681TrpGly: 1.681 ± 0.262
0.48TrpHis: 0.48 ± 0.159
1.26TrpIle: 1.26 ± 0.261
0.3TrpLys: 0.3 ± 0.173
1.681TrpLeu: 1.681 ± 0.294
0.3TrpMet: 0.3 ± 0.132
0.36TrpAsn: 0.36 ± 0.135
0.96TrpPro: 0.96 ± 0.277
0.78TrpGln: 0.78 ± 0.189
1.26TrpArg: 1.26 ± 0.301
0.9TrpSer: 0.9 ± 0.266
1.621TrpThr: 1.621 ± 0.334
1.981TrpVal: 1.981 ± 0.293
0.6TrpTrp: 0.6 ± 0.224
0.24TrpTyr: 0.24 ± 0.106
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.281TyrAla: 2.281 ± 0.383
0.36TyrCys: 0.36 ± 0.17
1.26TyrAsp: 1.26 ± 0.278
2.041TyrGlu: 2.041 ± 0.344
0.6TyrPhe: 0.6 ± 0.149
2.521TyrGly: 2.521 ± 0.418
0.66TyrHis: 0.66 ± 0.201
1.861TyrIle: 1.861 ± 0.354
1.38TyrLys: 1.38 ± 0.253
2.041TyrLeu: 2.041 ± 0.361
0.72TyrMet: 0.72 ± 0.156
1.08TyrAsn: 1.08 ± 0.257
1.38TyrPro: 1.38 ± 0.287
1.2TyrGln: 1.2 ± 0.252
2.101TyrArg: 2.101 ± 0.314
1.44TyrSer: 1.44 ± 0.264
1.801TyrThr: 1.801 ± 0.341
2.161TyrVal: 2.161 ± 0.358
0.48TyrTrp: 0.48 ± 0.156
0.6TyrTyr: 0.6 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (16662 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski