Filimonas lacunae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Filimonas

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173MGZ2|A0A173MGZ2_9BACT Acyl dehydratase OS=Filimonas lacunae OX=477680 GN=SAMN05421788_107347 PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 5.37NKK4 pKa = 9.64QSNPYY9 pKa = 10.43YY10 pKa = 10.31IFTQEE15 pKa = 5.13ILDD18 pKa = 3.68SWVQEE23 pKa = 3.7GVQYY27 pKa = 11.29VKK29 pKa = 10.72VAEE32 pKa = 4.66LEE34 pKa = 4.09TGTQEE39 pKa = 4.8KK40 pKa = 10.19YY41 pKa = 10.56YY42 pKa = 10.7EE43 pKa = 5.27LIPDD47 pKa = 4.35PDD49 pKa = 3.92FMGGDD54 pKa = 3.8DD55 pKa = 4.75PIYY58 pKa = 10.21PIGAEE63 pKa = 4.53DD64 pKa = 3.15ITEE67 pKa = 3.98LVEE70 pKa = 4.2IVPHH74 pKa = 6.09AKK76 pKa = 9.84FVVHH80 pKa = 7.04EE81 pKa = 4.77IYY83 pKa = 10.95LDD85 pKa = 3.65MEE87 pKa = 4.78DD88 pKa = 4.18EE89 pKa = 4.38EE90 pKa = 4.65EE91 pKa = 4.05

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7R4I3|A0A1N7R4I3_9BACT Peroxiredoxin OS=Filimonas lacunae OX=477680 GN=SAMN05421788_109112 PE=4 SV=1
MM1 pKa = 6.82NHH3 pKa = 7.58KK4 pKa = 10.54ARR6 pKa = 11.84IWKK9 pKa = 10.08RR10 pKa = 11.84SVPPKK15 pKa = 9.98RR16 pKa = 11.84VLAIRR21 pKa = 11.84LQAMGDD27 pKa = 3.73LVITLPYY34 pKa = 10.06LQALRR39 pKa = 11.84DD40 pKa = 3.97SLPPGTQLDD49 pKa = 4.14LLTRR53 pKa = 11.84QEE55 pKa = 3.9VAAIPQHH62 pKa = 5.7LHH64 pKa = 5.19LFNRR68 pKa = 11.84VFAIGGGRR76 pKa = 11.84VFKK79 pKa = 10.83RR80 pKa = 11.84QFLYY84 pKa = 10.83AIFLLPRR91 pKa = 11.84LMLRR95 pKa = 11.84RR96 pKa = 11.84YY97 pKa = 10.22DD98 pKa = 4.15VVLDD102 pKa = 3.71LQNNKK107 pKa = 9.39LSRR110 pKa = 11.84MVRR113 pKa = 11.84LVLRR117 pKa = 11.84PRR119 pKa = 11.84AWTEE123 pKa = 3.34FDD125 pKa = 3.25RR126 pKa = 11.84LSPISAGLRR135 pKa = 11.84NQNTIAAVGLGNHH148 pKa = 6.81AAAFHH153 pKa = 6.26LCLRR157 pKa = 11.84EE158 pKa = 4.05PVDD161 pKa = 3.34TAGLLRR167 pKa = 11.84RR168 pKa = 11.84NGWDD172 pKa = 3.33GVSGLVVLNPAGAFATRR189 pKa = 11.84HH190 pKa = 4.86WPVEE194 pKa = 4.12NYY196 pKa = 10.23VSFARR201 pKa = 11.84QWLQHH206 pKa = 5.91FPQTQFLVIGVPLVAEE222 pKa = 4.23KK223 pKa = 10.05ATFFQRR229 pKa = 11.84QLGGRR234 pKa = 11.84LINLVSKK241 pKa = 6.33TTPVEE246 pKa = 4.11AFALLQQVRR255 pKa = 11.84LTLTEE260 pKa = 4.53DD261 pKa = 3.14SGLMHH266 pKa = 6.7MSWVQGIATLALFGSTRR283 pKa = 11.84SDD285 pKa = 2.45WSAPLGRR292 pKa = 11.84HH293 pKa = 5.34SLLLHH298 pKa = 6.79SGDD301 pKa = 4.59LPCGSCMRR309 pKa = 11.84EE310 pKa = 3.71QCLHH314 pKa = 6.45GNTPCLTRR322 pKa = 11.84YY323 pKa = 7.98TPEE326 pKa = 3.8QVLAAALALVAAATAGAA343 pKa = 3.97

Molecular weight: 38.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6193

0

6193

2362209

26

6707

381.4

42.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.383 ± 0.036

0.896 ± 0.011

4.929 ± 0.023

5.111 ± 0.044

4.433 ± 0.024

6.969 ± 0.035

1.984 ± 0.019

6.33 ± 0.027

6.053 ± 0.041

9.291 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.018

5.487 ± 0.029

3.923 ± 0.019

4.304 ± 0.025

3.96 ± 0.028

6.424 ± 0.036

6.649 ± 0.07

6.879 ± 0.037

1.308 ± 0.011

4.423 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski