Hubei sobemo-like virus 20

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEC2|A0A1L3KEC2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 20 OX=1923206 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84ALQRR6 pKa = 11.84LDD8 pKa = 4.62LNSSPGYY15 pKa = 9.61PYY17 pKa = 10.83LRR19 pKa = 11.84QATNNRR25 pKa = 11.84QLFTDD30 pKa = 3.6VDD32 pKa = 4.72GNWLPDD38 pKa = 3.37RR39 pKa = 11.84VEE41 pKa = 3.98WCWDD45 pKa = 3.3LVSRR49 pKa = 11.84RR50 pKa = 11.84LGNLSSADD58 pKa = 4.02PIRR61 pKa = 11.84LFVKK65 pKa = 10.16PEE67 pKa = 3.6PHH69 pKa = 6.4SLKK72 pKa = 10.58KK73 pKa = 9.76MHH75 pKa = 7.25DD76 pKa = 2.93KK77 pKa = 10.48RR78 pKa = 11.84YY79 pKa = 9.87RR80 pKa = 11.84LISSVSVVDD89 pKa = 4.35QIIDD93 pKa = 3.32HH94 pKa = 6.57MIFGDD99 pKa = 5.24LNDD102 pKa = 4.55LLIKK106 pKa = 9.57NWHH109 pKa = 6.35VIPSKK114 pKa = 10.35PGWSFLHH121 pKa = 6.56GGWKK125 pKa = 9.13MMPPKK130 pKa = 10.11GKK132 pKa = 8.94WVAIDD137 pKa = 3.46KK138 pKa = 10.87SSWDD142 pKa = 3.16WTVQLWLLEE151 pKa = 4.2MVLEE155 pKa = 4.37ARR157 pKa = 11.84LRR159 pKa = 11.84LCRR162 pKa = 11.84TTGEE166 pKa = 4.19LRR168 pKa = 11.84NRR170 pKa = 11.84WFKK173 pKa = 8.83YY174 pKa = 8.94AYY176 pKa = 10.14YY177 pKa = 10.34RR178 pKa = 11.84FEE180 pKa = 4.05QLYY183 pKa = 8.81LKK185 pKa = 10.62CVFITSGGHH194 pKa = 6.58LLRR197 pKa = 11.84QLRR200 pKa = 11.84PGALKK205 pKa = 10.42SGCVNTIADD214 pKa = 4.17NSLMQYY220 pKa = 10.53LLHH223 pKa = 6.41LRR225 pKa = 11.84VVFQLGYY232 pKa = 9.76SPGIIMSMGDD242 pKa = 3.25DD243 pKa = 3.61TLQQEE248 pKa = 4.59PEE250 pKa = 3.92DD251 pKa = 3.66LQRR254 pKa = 11.84YY255 pKa = 6.96IQEE258 pKa = 4.26LQTFCIVKK266 pKa = 9.39QYY268 pKa = 10.49EE269 pKa = 4.2RR270 pKa = 11.84LVEE273 pKa = 3.92FAGFRR278 pKa = 11.84FDD280 pKa = 4.19GMQVEE285 pKa = 4.35PSYY288 pKa = 10.38RR289 pKa = 11.84AKK291 pKa = 10.7HH292 pKa = 5.61SFNLLHH298 pKa = 7.14VDD300 pKa = 3.91DD301 pKa = 5.74AVLPDD306 pKa = 3.37VAVAYY311 pKa = 10.84SLMYY315 pKa = 10.0HH316 pKa = 6.21RR317 pKa = 11.84SKK319 pKa = 10.43YY320 pKa = 9.77RR321 pKa = 11.84VWIRR325 pKa = 11.84SLFEE329 pKa = 4.1EE330 pKa = 4.68MGLDD334 pKa = 4.04LPSLEE339 pKa = 4.19YY340 pKa = 10.47FDD342 pKa = 6.4AIFDD346 pKa = 4.2GEE348 pKa = 4.43VV349 pKa = 2.64

Molecular weight: 41.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEC2|A0A1L3KEC2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 20 OX=1923206 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 6.3AEE4 pKa = 4.0QQLQQEE10 pKa = 4.85KK11 pKa = 10.25YY12 pKa = 9.65LLDD15 pKa = 3.64VMVDD19 pKa = 3.04KK20 pKa = 10.71VTVIGRR26 pKa = 11.84RR27 pKa = 11.84IMMAYY32 pKa = 9.45KK33 pKa = 9.39LTVNAVFLFSVYY45 pKa = 9.69SWICRR50 pKa = 11.84HH51 pKa = 5.96KK52 pKa = 11.11EE53 pKa = 3.42MVIRR57 pKa = 11.84PKK59 pKa = 10.57SRR61 pKa = 11.84YY62 pKa = 7.68EE63 pKa = 3.9LLWEE67 pKa = 3.93LVIEE71 pKa = 4.64HH72 pKa = 6.11SQPLVVVGVVVASIVVALLVRR93 pKa = 11.84RR94 pKa = 11.84SRR96 pKa = 11.84RR97 pKa = 11.84PVRR100 pKa = 11.84RR101 pKa = 11.84AIMRR105 pKa = 11.84LRR107 pKa = 11.84GVSLEE112 pKa = 3.8AMRR115 pKa = 11.84EE116 pKa = 4.03GSDD119 pKa = 4.21FRR121 pKa = 11.84AGDD124 pKa = 3.39QPKK127 pKa = 9.63FQVAILRR134 pKa = 11.84PGLLSDD140 pKa = 3.03SHH142 pKa = 7.92YY143 pKa = 10.98GYY145 pKa = 10.8GIRR148 pKa = 11.84VGDD151 pKa = 3.64FLVTPEE157 pKa = 4.34HH158 pKa = 6.33VVAGMPEE165 pKa = 4.33VILSANGNKK174 pKa = 9.03VQVPTVPMLRR184 pKa = 11.84SRR186 pKa = 11.84AFSDD190 pKa = 3.37LVYY193 pKa = 10.71IRR195 pKa = 11.84VSDD198 pKa = 4.08VAWSRR203 pKa = 11.84LAIRR207 pKa = 11.84TAVLSQKK214 pKa = 9.16TMATNLFVSVYY225 pKa = 7.93GQKK228 pKa = 10.56GVSSGLLSKK237 pKa = 10.85SQMVGLLVYY246 pKa = 10.36HH247 pKa = 7.03GSTVPGMSGAAYY259 pKa = 8.19TVGNAVYY266 pKa = 9.8GIHH269 pKa = 6.7CGVVANEE276 pKa = 3.92NMGVASLLLAAEE288 pKa = 5.12LKK290 pKa = 10.4RR291 pKa = 11.84YY292 pKa = 8.48MIGEE296 pKa = 4.07ASEE299 pKa = 5.38DD300 pKa = 3.71FAAQDD305 pKa = 2.96IWKK308 pKa = 9.03SQPKK312 pKa = 8.35KK313 pKa = 8.65TWDD316 pKa = 3.29SSDD319 pKa = 3.15VFKK322 pKa = 11.28VVEE325 pKa = 4.16RR326 pKa = 11.84RR327 pKa = 11.84FKK329 pKa = 10.62YY330 pKa = 8.82TDD332 pKa = 3.32EE333 pKa = 4.43EE334 pKa = 4.11QGEE337 pKa = 4.32MLARR341 pKa = 11.84GATWGQVCEE350 pKa = 4.18WADD353 pKa = 3.55EE354 pKa = 4.59SIAGAMATKK363 pKa = 10.01PKK365 pKa = 9.17TVKK368 pKa = 9.95PHH370 pKa = 4.23VTIEE374 pKa = 4.31DD375 pKa = 3.37GTIAMKK381 pKa = 8.62MHH383 pKa = 6.45PQGGSEE389 pKa = 4.28SGEE392 pKa = 3.91EE393 pKa = 3.86MTILAQLPAGASPKK407 pKa = 10.61ASNTYY412 pKa = 8.47QAEE415 pKa = 4.08EE416 pKa = 4.39VIADD420 pKa = 3.92LVRR423 pKa = 11.84RR424 pKa = 11.84VLEE427 pKa = 3.81LEE429 pKa = 3.69RR430 pKa = 11.84RR431 pKa = 11.84MDD433 pKa = 3.74RR434 pKa = 11.84VEE436 pKa = 4.14HH437 pKa = 6.23PMVEE441 pKa = 4.35VPLPEE446 pKa = 4.87AKK448 pKa = 10.07RR449 pKa = 11.84QFYY452 pKa = 10.28SCTMCSGKK460 pKa = 8.73FTDD463 pKa = 4.57PVAHH467 pKa = 6.75ARR469 pKa = 11.84LHH471 pKa = 6.02EE472 pKa = 4.43KK473 pKa = 9.88PICPQCGISCTDD485 pKa = 3.17EE486 pKa = 3.87QAVRR490 pKa = 11.84NHH492 pKa = 5.68LRR494 pKa = 11.84ASHH497 pKa = 5.93PQRR500 pKa = 11.84IQCDD504 pKa = 3.46RR505 pKa = 11.84CGVTCRR511 pKa = 11.84SSEE514 pKa = 3.69RR515 pKa = 11.84LARR518 pKa = 11.84HH519 pKa = 6.3RR520 pKa = 11.84KK521 pKa = 6.66TCIPMTDD528 pKa = 3.01GSFTKK533 pKa = 10.48EE534 pKa = 3.77SADD537 pKa = 3.38QVDD540 pKa = 3.31SRR542 pKa = 11.84KK543 pKa = 9.67VVRR546 pKa = 11.84TAGAFLGKK554 pKa = 9.71KK555 pKa = 9.73ASRR558 pKa = 11.84ARR560 pKa = 11.84RR561 pKa = 11.84SASSTRR567 pKa = 11.84SSSSSDD573 pKa = 3.01KK574 pKa = 11.26SPPCPSRR581 pKa = 11.84QDD583 pKa = 3.56YY584 pKa = 11.1LKK586 pKa = 10.92LILEE590 pKa = 4.63SQRR593 pKa = 11.84STQLALEE600 pKa = 4.41NLLSSMTGPSSGILQSS616 pKa = 3.4

Molecular weight: 68.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

965

349

616

482.5

54.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.839 ± 1.608

1.865 ± 0.246

5.078 ± 1.023

5.596 ± 0.575

3.109 ± 0.839

6.218 ± 0.277

2.487 ± 0.215

4.352 ± 0.132

4.87 ± 0.163

9.948 ± 1.838

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.731 ± 0.166

2.176 ± 0.555

4.56 ± 0.014

4.56 ± 0.014

7.772 ± 0.183

8.705 ± 1.039

3.938 ± 0.773

8.912 ± 1.157

1.969 ± 0.835

3.316 ± 0.721
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski