Amino acid dipepetide frequency for Delftia phage IME-DE1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.269AlaAla: 15.269 ± 1.989
0.868AlaCys: 0.868 ± 0.242
6.593AlaAsp: 6.593 ± 0.901
6.767AlaGlu: 6.767 ± 0.757
3.817AlaPhe: 3.817 ± 0.567
8.589AlaGly: 8.589 ± 0.736
2.689AlaHis: 2.689 ± 0.687
5.292AlaIle: 5.292 ± 0.581
7.114AlaLys: 7.114 ± 0.924
8.936AlaLeu: 8.936 ± 1.118
2.603AlaMet: 2.603 ± 0.445
3.817AlaAsn: 3.817 ± 0.876
4.077AlaPro: 4.077 ± 0.539
5.032AlaGln: 5.032 ± 0.762
6.246AlaArg: 6.246 ± 0.537
6.159AlaSer: 6.159 ± 0.912
5.899AlaThr: 5.899 ± 1.039
7.547AlaVal: 7.547 ± 0.786
1.735AlaTrp: 1.735 ± 0.356
3.297AlaTyr: 3.297 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
0.954CysAla: 0.954 ± 0.303
0.087CysCys: 0.087 ± 0.088
0.694CysAsp: 0.694 ± 0.217
0.868CysGlu: 0.868 ± 0.328
0.434CysPhe: 0.434 ± 0.22
0.521CysGly: 0.521 ± 0.25
0.347CysHis: 0.347 ± 0.153
0.434CysIle: 0.434 ± 0.18
0.521CysLys: 0.521 ± 0.215
1.215CysLeu: 1.215 ± 0.361
0.174CysMet: 0.174 ± 0.14
0.174CysAsn: 0.174 ± 0.108
0.521CysPro: 0.521 ± 0.253
0.26CysGln: 0.26 ± 0.155
0.521CysArg: 0.521 ± 0.281
0.607CysSer: 0.607 ± 0.228
0.174CysThr: 0.174 ± 0.13
0.434CysVal: 0.434 ± 0.224
0.26CysTrp: 0.26 ± 0.13
0.26CysTyr: 0.26 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
7.2AspAla: 7.2 ± 0.872
0.521AspCys: 0.521 ± 0.229
3.817AspAsp: 3.817 ± 0.624
4.077AspGlu: 4.077 ± 0.558
1.995AspPhe: 1.995 ± 0.504
5.205AspGly: 5.205 ± 0.789
1.301AspHis: 1.301 ± 0.358
2.603AspIle: 2.603 ± 0.454
2.863AspLys: 2.863 ± 0.444
5.465AspLeu: 5.465 ± 0.709
1.041AspMet: 1.041 ± 0.287
1.475AspAsn: 1.475 ± 0.354
3.47AspPro: 3.47 ± 0.581
2.95AspGln: 2.95 ± 0.598
2.516AspArg: 2.516 ± 0.308
3.297AspSer: 3.297 ± 0.48
3.036AspThr: 3.036 ± 0.363
3.123AspVal: 3.123 ± 0.617
0.607AspTrp: 0.607 ± 0.244
1.562AspTyr: 1.562 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
8.415GluAla: 8.415 ± 1.073
0.694GluCys: 0.694 ± 0.285
4.771GluAsp: 4.771 ± 0.921
3.47GluGlu: 3.47 ± 0.632
3.21GluPhe: 3.21 ± 0.51
4.338GluGly: 4.338 ± 0.842
0.868GluHis: 0.868 ± 0.252
1.909GluIle: 1.909 ± 0.388
3.644GluLys: 3.644 ± 0.638
3.817GluLeu: 3.817 ± 0.599
0.521GluMet: 0.521 ± 0.179
1.822GluAsn: 1.822 ± 0.398
2.603GluPro: 2.603 ± 0.474
3.297GluGln: 3.297 ± 0.594
3.991GluArg: 3.991 ± 0.548
4.164GluSer: 4.164 ± 0.518
3.47GluThr: 3.47 ± 0.498
5.032GluVal: 5.032 ± 0.726
1.301GluTrp: 1.301 ± 0.367
2.256GluTyr: 2.256 ± 0.445
0.0GluXaa: 0.0 ± 0.0
Phe
2.689PheAla: 2.689 ± 0.547
0.434PheCys: 0.434 ± 0.181
2.689PheAsp: 2.689 ± 0.499
1.562PheGlu: 1.562 ± 0.433
1.562PhePhe: 1.562 ± 0.4
2.256PheGly: 2.256 ± 0.465
0.781PheHis: 0.781 ± 0.281
1.735PheIle: 1.735 ± 0.46
2.256PheLys: 2.256 ± 0.449
2.342PheLeu: 2.342 ± 0.536
1.388PheMet: 1.388 ± 0.342
2.256PheAsn: 2.256 ± 0.46
1.995PhePro: 1.995 ± 0.527
1.909PheGln: 1.909 ± 0.424
2.169PheArg: 2.169 ± 0.596
1.995PheSer: 1.995 ± 0.455
1.995PheThr: 1.995 ± 0.463
2.603PheVal: 2.603 ± 0.565
0.347PheTrp: 0.347 ± 0.161
0.954PheTyr: 0.954 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
8.242GlyAla: 8.242 ± 0.981
0.434GlyCys: 0.434 ± 0.171
5.205GlyAsp: 5.205 ± 0.704
4.511GlyGlu: 4.511 ± 0.566
3.123GlyPhe: 3.123 ± 0.428
6.853GlyGly: 6.853 ± 0.897
1.648GlyHis: 1.648 ± 0.443
3.73GlyIle: 3.73 ± 0.528
5.812GlyLys: 5.812 ± 0.7
7.721GlyLeu: 7.721 ± 0.915
2.256GlyMet: 2.256 ± 0.387
2.082GlyAsn: 2.082 ± 0.39
2.689GlyPro: 2.689 ± 0.561
3.297GlyGln: 3.297 ± 0.705
3.991GlyArg: 3.991 ± 0.533
5.726GlySer: 5.726 ± 0.577
5.032GlyThr: 5.032 ± 0.907
4.858GlyVal: 4.858 ± 0.895
2.256GlyTrp: 2.256 ± 0.444
3.297GlyTyr: 3.297 ± 0.609
0.0GlyXaa: 0.0 ± 0.0
His
1.562HisAla: 1.562 ± 0.35
0.434HisCys: 0.434 ± 0.193
1.215HisAsp: 1.215 ± 0.41
1.041HisGlu: 1.041 ± 0.423
0.694HisPhe: 0.694 ± 0.265
1.562HisGly: 1.562 ± 0.371
0.434HisHis: 0.434 ± 0.215
1.128HisIle: 1.128 ± 0.352
0.954HisLys: 0.954 ± 0.309
1.562HisLeu: 1.562 ± 0.364
0.607HisMet: 0.607 ± 0.217
1.128HisAsn: 1.128 ± 0.271
1.215HisPro: 1.215 ± 0.335
0.694HisGln: 0.694 ± 0.256
1.388HisArg: 1.388 ± 0.333
0.954HisSer: 0.954 ± 0.273
0.694HisThr: 0.694 ± 0.228
1.215HisVal: 1.215 ± 0.391
0.347HisTrp: 0.347 ± 0.169
0.347HisTyr: 0.347 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
5.205IleAla: 5.205 ± 0.836
0.521IleCys: 0.521 ± 0.205
3.036IleAsp: 3.036 ± 0.424
2.689IleGlu: 2.689 ± 0.416
1.215IlePhe: 1.215 ± 0.331
3.557IleGly: 3.557 ± 0.741
0.954IleHis: 0.954 ± 0.341
2.95IleIle: 2.95 ± 0.545
2.429IleLys: 2.429 ± 0.639
2.429IleLeu: 2.429 ± 0.598
0.781IleMet: 0.781 ± 0.243
1.301IleAsn: 1.301 ± 0.295
2.689IlePro: 2.689 ± 0.438
2.429IleGln: 2.429 ± 0.425
3.904IleArg: 3.904 ± 0.526
1.822IleSer: 1.822 ± 0.403
2.776IleThr: 2.776 ± 0.455
3.73IleVal: 3.73 ± 0.564
0.781IleTrp: 0.781 ± 0.247
0.954IleTyr: 0.954 ± 0.267
0.0IleXaa: 0.0 ± 0.0
Lys
8.502LysAla: 8.502 ± 1.541
0.781LysCys: 0.781 ± 0.259
4.077LysAsp: 4.077 ± 0.508
4.424LysGlu: 4.424 ± 0.653
1.735LysPhe: 1.735 ± 0.416
5.726LysGly: 5.726 ± 0.739
1.128LysHis: 1.128 ± 0.26
1.822LysIle: 1.822 ± 0.301
2.776LysLys: 2.776 ± 0.455
5.292LysLeu: 5.292 ± 0.768
1.215LysMet: 1.215 ± 0.322
1.909LysAsn: 1.909 ± 0.32
1.995LysPro: 1.995 ± 0.549
2.95LysGln: 2.95 ± 0.49
3.297LysArg: 3.297 ± 0.625
2.603LysSer: 2.603 ± 0.373
3.73LysThr: 3.73 ± 0.78
4.338LysVal: 4.338 ± 0.52
1.128LysTrp: 1.128 ± 0.39
1.562LysTyr: 1.562 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
6.767LeuAla: 6.767 ± 0.796
0.694LeuCys: 0.694 ± 0.253
3.297LeuAsp: 3.297 ± 0.502
6.506LeuGlu: 6.506 ± 1.072
1.995LeuPhe: 1.995 ± 0.392
7.027LeuGly: 7.027 ± 0.82
1.041LeuHis: 1.041 ± 0.324
3.73LeuIle: 3.73 ± 0.695
6.333LeuLys: 6.333 ± 0.848
5.379LeuLeu: 5.379 ± 0.86
2.169LeuMet: 2.169 ± 0.442
4.077LeuAsn: 4.077 ± 0.572
3.73LeuPro: 3.73 ± 0.49
3.991LeuGln: 3.991 ± 0.645
4.511LeuArg: 4.511 ± 0.675
4.858LeuSer: 4.858 ± 0.856
3.557LeuThr: 3.557 ± 0.605
5.465LeuVal: 5.465 ± 0.811
1.735LeuTrp: 1.735 ± 0.419
2.95LeuTyr: 2.95 ± 0.478
0.0LeuXaa: 0.0 ± 0.0
Met
3.123MetAla: 3.123 ± 0.391
0.174MetCys: 0.174 ± 0.142
1.215MetAsp: 1.215 ± 0.411
1.475MetGlu: 1.475 ± 0.389
0.607MetPhe: 0.607 ± 0.294
1.909MetGly: 1.909 ± 0.444
0.521MetHis: 0.521 ± 0.194
1.215MetIle: 1.215 ± 0.333
1.735MetLys: 1.735 ± 0.349
1.648MetLeu: 1.648 ± 0.325
0.694MetMet: 0.694 ± 0.258
1.301MetAsn: 1.301 ± 0.314
1.562MetPro: 1.562 ± 0.393
0.694MetGln: 0.694 ± 0.32
0.954MetArg: 0.954 ± 0.351
2.082MetSer: 2.082 ± 0.456
1.388MetThr: 1.388 ± 0.442
1.215MetVal: 1.215 ± 0.296
0.174MetTrp: 0.174 ± 0.109
0.347MetTyr: 0.347 ± 0.217
0.0MetXaa: 0.0 ± 0.0
Asn
5.812AsnAla: 5.812 ± 0.884
0.174AsnCys: 0.174 ± 0.114
2.169AsnAsp: 2.169 ± 0.417
2.429AsnGlu: 2.429 ± 0.503
1.388AsnPhe: 1.388 ± 0.237
4.945AsnGly: 4.945 ± 0.742
0.607AsnHis: 0.607 ± 0.215
1.562AsnIle: 1.562 ± 0.341
1.388AsnLys: 1.388 ± 0.308
3.47AsnLeu: 3.47 ± 0.563
0.607AsnMet: 0.607 ± 0.222
0.868AsnAsn: 0.868 ± 0.25
3.21AsnPro: 3.21 ± 0.537
1.128AsnGln: 1.128 ± 0.283
1.475AsnArg: 1.475 ± 0.353
1.301AsnSer: 1.301 ± 0.423
1.822AsnThr: 1.822 ± 0.475
2.082AsnVal: 2.082 ± 0.33
0.781AsnTrp: 0.781 ± 0.347
1.388AsnTyr: 1.388 ± 0.315
0.0AsnXaa: 0.0 ± 0.0
Pro
3.991ProAla: 3.991 ± 0.663
0.26ProCys: 0.26 ± 0.168
2.256ProAsp: 2.256 ± 0.42
3.644ProGlu: 3.644 ± 0.685
1.995ProPhe: 1.995 ± 0.367
4.685ProGly: 4.685 ± 0.54
1.041ProHis: 1.041 ± 0.292
1.301ProIle: 1.301 ± 0.319
3.383ProLys: 3.383 ± 0.66
2.776ProLeu: 2.776 ± 0.505
0.781ProMet: 0.781 ± 0.276
2.516ProAsn: 2.516 ± 0.349
1.388ProPro: 1.388 ± 0.267
2.516ProGln: 2.516 ± 0.417
1.648ProArg: 1.648 ± 0.46
2.342ProSer: 2.342 ± 0.521
2.169ProThr: 2.169 ± 0.344
3.817ProVal: 3.817 ± 0.586
0.694ProTrp: 0.694 ± 0.243
1.909ProTyr: 1.909 ± 0.507
0.0ProXaa: 0.0 ± 0.0
Gln
5.292GlnAla: 5.292 ± 0.845
0.434GlnCys: 0.434 ± 0.216
2.429GlnAsp: 2.429 ± 0.687
2.863GlnGlu: 2.863 ± 0.503
1.995GlnPhe: 1.995 ± 0.336
3.47GlnGly: 3.47 ± 0.511
1.475GlnHis: 1.475 ± 0.314
1.041GlnIle: 1.041 ± 0.268
2.603GlnLys: 2.603 ± 0.513
3.73GlnLeu: 3.73 ± 0.397
0.868GlnMet: 0.868 ± 0.254
1.735GlnAsn: 1.735 ± 0.497
0.868GlnPro: 0.868 ± 0.24
2.429GlnGln: 2.429 ± 0.648
2.863GlnArg: 2.863 ± 0.537
2.342GlnSer: 2.342 ± 0.511
2.256GlnThr: 2.256 ± 0.502
3.817GlnVal: 3.817 ± 0.417
1.128GlnTrp: 1.128 ± 0.31
1.388GlnTyr: 1.388 ± 0.396
0.0GlnXaa: 0.0 ± 0.0
Arg
5.465ArgAla: 5.465 ± 0.545
0.347ArgCys: 0.347 ± 0.185
3.383ArgAsp: 3.383 ± 0.489
3.557ArgGlu: 3.557 ± 0.513
2.342ArgPhe: 2.342 ± 0.448
3.47ArgGly: 3.47 ± 0.551
0.694ArgHis: 0.694 ± 0.192
3.557ArgIle: 3.557 ± 0.747
3.817ArgLys: 3.817 ± 0.724
4.077ArgLeu: 4.077 ± 0.479
1.301ArgMet: 1.301 ± 0.318
2.603ArgAsn: 2.603 ± 0.445
1.822ArgPro: 1.822 ± 0.354
2.603ArgGln: 2.603 ± 0.697
2.342ArgArg: 2.342 ± 0.352
3.557ArgSer: 3.557 ± 0.522
2.516ArgThr: 2.516 ± 0.457
4.251ArgVal: 4.251 ± 0.567
0.868ArgTrp: 0.868 ± 0.389
1.735ArgTyr: 1.735 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
6.42SerAla: 6.42 ± 1.461
0.521SerCys: 0.521 ± 0.212
3.036SerAsp: 3.036 ± 0.677
2.603SerGlu: 2.603 ± 0.378
2.429SerPhe: 2.429 ± 0.442
5.465SerGly: 5.465 ± 0.797
0.781SerHis: 0.781 ± 0.308
3.991SerIle: 3.991 ± 0.5
3.036SerLys: 3.036 ± 0.627
4.338SerLeu: 4.338 ± 0.43
1.822SerMet: 1.822 ± 0.415
2.256SerAsn: 2.256 ± 0.437
2.689SerPro: 2.689 ± 0.557
1.995SerGln: 1.995 ± 0.408
2.516SerArg: 2.516 ± 0.395
2.863SerSer: 2.863 ± 0.353
3.297SerThr: 3.297 ± 0.604
3.73SerVal: 3.73 ± 0.625
1.215SerTrp: 1.215 ± 0.345
1.475SerTyr: 1.475 ± 0.254
0.0SerXaa: 0.0 ± 0.0
Thr
5.032ThrAla: 5.032 ± 0.833
0.954ThrCys: 0.954 ± 0.272
2.429ThrAsp: 2.429 ± 0.358
3.904ThrGlu: 3.904 ± 0.726
1.822ThrPhe: 1.822 ± 0.499
4.945ThrGly: 4.945 ± 0.718
1.041ThrHis: 1.041 ± 0.303
3.036ThrIle: 3.036 ± 0.508
1.822ThrLys: 1.822 ± 0.395
4.598ThrLeu: 4.598 ± 0.589
1.215ThrMet: 1.215 ± 0.384
2.082ThrAsn: 2.082 ± 0.592
3.297ThrPro: 3.297 ± 0.486
2.082ThrGln: 2.082 ± 0.456
3.73ThrArg: 3.73 ± 0.644
2.516ThrSer: 2.516 ± 0.598
2.863ThrThr: 2.863 ± 0.698
3.557ThrVal: 3.557 ± 0.628
0.694ThrTrp: 0.694 ± 0.307
1.215ThrTyr: 1.215 ± 0.324
0.0ThrXaa: 0.0 ± 0.0
Val
8.242ValAla: 8.242 ± 0.713
0.607ValCys: 0.607 ± 0.244
2.863ValAsp: 2.863 ± 0.535
4.251ValGlu: 4.251 ± 0.679
2.256ValPhe: 2.256 ± 0.444
4.164ValGly: 4.164 ± 0.536
1.128ValHis: 1.128 ± 0.386
3.383ValIle: 3.383 ± 0.583
5.118ValLys: 5.118 ± 0.835
6.333ValLeu: 6.333 ± 0.655
2.429ValMet: 2.429 ± 0.346
3.297ValAsn: 3.297 ± 0.517
3.123ValPro: 3.123 ± 0.459
2.776ValGln: 2.776 ± 0.525
3.297ValArg: 3.297 ± 0.61
4.771ValSer: 4.771 ± 0.825
3.21ValThr: 3.21 ± 0.49
4.077ValVal: 4.077 ± 0.539
1.041ValTrp: 1.041 ± 0.27
1.215ValTyr: 1.215 ± 0.25
0.0ValXaa: 0.0 ± 0.0
Trp
1.735TrpAla: 1.735 ± 0.416
0.174TrpCys: 0.174 ± 0.13
0.954TrpAsp: 0.954 ± 0.382
1.041TrpGlu: 1.041 ± 0.343
0.434TrpPhe: 0.434 ± 0.206
1.128TrpGly: 1.128 ± 0.271
0.434TrpHis: 0.434 ± 0.203
0.347TrpIle: 0.347 ± 0.162
1.735TrpLys: 1.735 ± 0.462
2.429TrpLeu: 2.429 ± 0.491
0.868TrpMet: 0.868 ± 0.244
0.694TrpAsn: 0.694 ± 0.183
0.694TrpPro: 0.694 ± 0.245
0.607TrpGln: 0.607 ± 0.253
0.868TrpArg: 0.868 ± 0.228
0.954TrpSer: 0.954 ± 0.286
1.215TrpThr: 1.215 ± 0.405
0.954TrpVal: 0.954 ± 0.376
0.607TrpTrp: 0.607 ± 0.203
0.607TrpTyr: 0.607 ± 0.204
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.429TyrAla: 2.429 ± 0.375
0.434TyrCys: 0.434 ± 0.171
1.995TyrAsp: 1.995 ± 0.272
1.909TyrGlu: 1.909 ± 0.442
0.868TyrPhe: 0.868 ± 0.249
2.95TyrGly: 2.95 ± 0.621
0.26TyrHis: 0.26 ± 0.165
1.388TyrIle: 1.388 ± 0.388
1.648TyrLys: 1.648 ± 0.383
2.256TyrLeu: 2.256 ± 0.381
0.607TyrMet: 0.607 ± 0.227
1.215TyrAsn: 1.215 ± 0.327
1.475TyrPro: 1.475 ± 0.53
1.388TyrGln: 1.388 ± 0.374
2.082TyrArg: 2.082 ± 0.374
1.562TyrSer: 1.562 ± 0.368
1.735TyrThr: 1.735 ± 0.425
1.735TyrVal: 1.735 ± 0.271
0.781TyrTrp: 0.781 ± 0.241
0.521TyrTyr: 0.521 ± 0.208
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (11528 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski