Neocallimastix californiae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Neocallimastigomycetes; Neocallimastigales; Neocallimastigaceae; Neocallimastix

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2FKW6|A0A1Y2FKW6_9FUNG Uncharacterized protein OS=Neocallimastix californiae OX=1754190 GN=LY90DRAFT_663591 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.18FSLVFGIVSAFVIVTNAYY20 pKa = 7.83PIPCFPGEE28 pKa = 3.87FGGNKK33 pKa = 9.24GKK35 pKa = 9.31PAPPFGGWPGAGNKK49 pKa = 10.09GNGGWPAPPNGGWPGNGDD67 pKa = 3.4KK68 pKa = 11.38GKK70 pKa = 10.89GGWPNNPWDD79 pKa = 4.37NKK81 pKa = 10.98NKK83 pKa = 9.83GDD85 pKa = 4.56KK86 pKa = 11.02NKK88 pKa = 10.45GDD90 pKa = 3.72KK91 pKa = 11.07NKK93 pKa = 10.45GDD95 pKa = 3.72KK96 pKa = 11.07NKK98 pKa = 10.45GDD100 pKa = 3.75KK101 pKa = 10.99NKK103 pKa = 10.65GDD105 pKa = 3.93GKK107 pKa = 11.07GEE109 pKa = 4.13EE110 pKa = 4.85CGPTSPNGPCKK121 pKa = 10.31PEE123 pKa = 4.23TPPSPPTIPLPPSPPSPPSPPSPPSPPSPPNGNNDD158 pKa = 3.03KK159 pKa = 11.52GEE161 pKa = 4.14AEE163 pKa = 4.15EE164 pKa = 5.27CGPDD168 pKa = 3.94SPNGPCEE175 pKa = 4.26PDD177 pKa = 3.6TPSTPPTPPSPPNGDD192 pKa = 3.31NDD194 pKa = 3.7TGEE197 pKa = 4.61AEE199 pKa = 4.26EE200 pKa = 5.28CGPDD204 pKa = 3.94SPNGPCEE211 pKa = 4.17PDD213 pKa = 3.67TPPSPPSLPSLPNGDD228 pKa = 3.63NDD230 pKa = 3.2IGEE233 pKa = 4.56AEE235 pKa = 4.22EE236 pKa = 5.14CGPDD240 pKa = 3.79SPKK243 pKa = 10.73GPCEE247 pKa = 4.09PEE249 pKa = 4.32SPPTPPNSPCDD260 pKa = 3.64PTTDD264 pKa = 3.9PSCAPEE270 pKa = 4.19PLCDD274 pKa = 3.63PTTDD278 pKa = 3.92PSCAPEE284 pKa = 4.47PPCDD288 pKa = 3.65PTTDD292 pKa = 3.9PSCAPEE298 pKa = 4.47PPCDD302 pKa = 3.65PTTDD306 pKa = 3.9PSCAPEE312 pKa = 4.47PPCDD316 pKa = 3.65PTTDD320 pKa = 3.9PSCAPEE326 pKa = 4.24PSCDD330 pKa = 3.53PSSDD334 pKa = 4.01PNCGNSDD341 pKa = 4.02PNEE344 pKa = 4.42DD345 pKa = 4.09PADD348 pKa = 4.0LLQVSEE354 pKa = 5.18DD355 pKa = 3.76EE356 pKa = 4.74NIGNEE361 pKa = 4.5DD362 pKa = 3.34IEE364 pKa = 4.54EE365 pKa = 4.29VNDD368 pKa = 3.78SNDD371 pKa = 2.99IVDD374 pKa = 3.94IANSSDD380 pKa = 3.41VEE382 pKa = 4.18

Molecular weight: 38.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2C741|A0A1Y2C741_9FUNG Brix-domain-containing protein OS=Neocallimastix californiae OX=1754190 GN=LY90DRAFT_458330 PE=4 SV=1
LL1 pKa = 7.19FRR3 pKa = 11.84INILFRR9 pKa = 11.84INILFRR15 pKa = 11.84INILFRR21 pKa = 11.84INILFRR27 pKa = 11.84INILFRR33 pKa = 11.84INILFRR39 pKa = 11.84INILFRR45 pKa = 11.84INILFRR51 pKa = 11.84INILFRR57 pKa = 11.84INILFRR63 pKa = 11.84INILFRR69 pKa = 11.84INILFRR75 pKa = 11.84INILFRR81 pKa = 11.84INILFRR87 pKa = 11.84INILFRR93 pKa = 11.84INILFRR99 pKa = 11.84INILFRR105 pKa = 11.84INILFRR111 pKa = 11.84INILFRR117 pKa = 11.84INILFRR123 pKa = 11.84INILFKK129 pKa = 10.82KK130 pKa = 10.35

Molecular weight: 16.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19950

14

19964

9707863

49

15892

486.3

55.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.757 ± 0.018

1.509 ± 0.013

5.716 ± 0.014

7.348 ± 0.024

4.271 ± 0.014

4.262 ± 0.021

1.65 ± 0.008

8.706 ± 0.025

8.972 ± 0.02

8.091 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.983 ± 0.008

9.958 ± 0.044

3.465 ± 0.015

3.242 ± 0.018

3.203 ± 0.014

8.52 ± 0.026

5.435 ± 0.018

4.567 ± 0.016

0.788 ± 0.007

4.557 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski