Amino acid dipepetide frequency for Neocallimastix californiae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.252AlaAla: 2.252 ± 0.027	0.64AlaCys: 0.64 ± 0.01	1.794AlaAsp: 1.794 ± 0.02	2.261AlaGlu: 2.261 ± 0.02	1.667AlaPhe: 1.667 ± 0.016	1.815AlaGly: 1.815 ± 0.025	0.559AlaHis: 0.559 ± 0.011	3.382AlaIle: 3.382 ± 0.021	3.067AlaLys: 3.067 ± 0.026	3.608AlaLeu: 3.608 ± 0.024	0.811AlaMet: 0.811 ± 0.013	2.666AlaAsn: 2.666 ± 0.021	1.376AlaPro: 1.376 ± 0.022	1.221AlaGln: 1.221 ± 0.019	1.198AlaArg: 1.198 ± 0.014	3.048AlaSer: 3.048 ± 0.025	2.33AlaThr: 2.33 ± 0.025	2.09AlaVal: 2.09 ± 0.018	0.318AlaTrp: 0.318 ± 0.008	1.472AlaTyr: 1.472 ± 0.013	0.0AlaXaa: 0.0 ± 0.0
Cys	0.526CysAla: 0.526 ± 0.01	0.458CysCys: 0.458 ± 0.011	0.807CysAsp: 0.807 ± 0.011	0.892CysGlu: 0.892 ± 0.016	0.642CysPhe: 0.642 ± 0.008	0.953CysGly: 0.953 ± 0.016	0.295CysHis: 0.295 ± 0.007	1.278CysIle: 1.278 ± 0.015	1.259CysLys: 1.259 ± 0.016	1.204CysLeu: 1.204 ± 0.014	0.268CysMet: 0.268 ± 0.006	1.085CysAsn: 1.085 ± 0.014	0.588CysPro: 0.588 ± 0.012	0.541CysGln: 0.541 ± 0.008	0.456CysArg: 0.456 ± 0.007	1.334CysSer: 1.334 ± 0.02	0.904CysThr: 0.904 ± 0.02	0.754CysVal: 0.754 ± 0.013	0.162CysTrp: 0.162 ± 0.005	0.685CysTyr: 0.685 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	2.017AspAla: 2.017 ± 0.019	0.739AspCys: 0.739 ± 0.015	4.51AspAsp: 4.51 ± 0.046	5.001AspGlu: 5.001 ± 0.035	2.5AspPhe: 2.5 ± 0.024	2.606AspGly: 2.606 ± 0.028	0.914AspHis: 0.914 ± 0.013	5.636AspIle: 5.636 ± 0.03	4.53AspLys: 4.53 ± 0.027	4.345AspLeu: 4.345 ± 0.031	1.045AspMet: 1.045 ± 0.012	5.427AspAsn: 5.427 ± 0.04	1.712AspPro: 1.712 ± 0.014	1.487AspGln: 1.487 ± 0.017	1.613AspArg: 1.613 ± 0.021	4.486AspSer: 4.486 ± 0.033	2.713AspThr: 2.713 ± 0.031	2.658AspVal: 2.658 ± 0.023	0.496AspTrp: 0.496 ± 0.008	2.722AspTyr: 2.722 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	2.696GluAla: 2.696 ± 0.027	1.044GluCys: 1.044 ± 0.017	4.596GluAsp: 4.596 ± 0.032	7.801GluGlu: 7.801 ± 0.097	2.767GluPhe: 2.767 ± 0.023	2.601GluGly: 2.601 ± 0.029	0.97GluHis: 0.97 ± 0.011	6.634GluIle: 6.634 ± 0.054	7.777GluLys: 7.777 ± 0.061	5.654GluLeu: 5.654 ± 0.035	1.505GluMet: 1.505 ± 0.015	7.789GluAsn: 7.789 ± 0.056	1.637GluPro: 1.637 ± 0.021	2.169GluGln: 2.169 ± 0.022	2.452GluArg: 2.452 ± 0.026	4.818GluSer: 4.818 ± 0.031	3.416GluThr: 3.416 ± 0.024	3.329GluVal: 3.329 ± 0.031	0.594GluTrp: 0.594 ± 0.01	3.229GluTyr: 3.229 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	1.509PheAla: 1.509 ± 0.014	0.615PheCys: 0.615 ± 0.009	2.633PheAsp: 2.633 ± 0.022	2.721PheGlu: 2.721 ± 0.02	1.983PhePhe: 1.983 ± 0.02	2.199PheGly: 2.199 ± 0.024	0.763PheHis: 0.763 ± 0.01	4.144PheIle: 4.144 ± 0.03	3.415PheLys: 3.415 ± 0.024	3.533PheLeu: 3.533 ± 0.028	0.893PheMet: 0.893 ± 0.01	3.908PheAsn: 3.908 ± 0.026	1.451PhePro: 1.451 ± 0.015	1.405PheGln: 1.405 ± 0.014	1.242PheArg: 1.242 ± 0.014	3.72PheSer: 3.72 ± 0.031	2.11PheThr: 2.11 ± 0.016	2.038PheVal: 2.038 ± 0.017	0.352PheTrp: 0.352 ± 0.006	2.075PheTyr: 2.075 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	1.865GlyAla: 1.865 ± 0.023	0.742GlyCys: 0.742 ± 0.013	2.234GlyAsp: 2.234 ± 0.021	2.607GlyGlu: 2.607 ± 0.025	1.918GlyPhe: 1.918 ± 0.02	2.536GlyGly: 2.536 ± 0.035	0.83GlyHis: 0.83 ± 0.01	3.684GlyIle: 3.684 ± 0.029	3.798GlyLys: 3.798 ± 0.03	3.183GlyLeu: 3.183 ± 0.026	0.938GlyMet: 0.938 ± 0.017	3.687GlyAsn: 3.687 ± 0.031	1.072GlyPro: 1.072 ± 0.018	1.212GlyGln: 1.212 ± 0.015	1.559GlyArg: 1.559 ± 0.019	3.388GlySer: 3.388 ± 0.033	2.33GlyThr: 2.33 ± 0.025	2.265GlyVal: 2.265 ± 0.022	0.469GlyTrp: 0.469 ± 0.01	2.304GlyTyr: 2.304 ± 0.025	0.0GlyXaa: 0.0 ± 0.0
His	0.547HisAla: 0.547 ± 0.008	0.351HisCys: 0.351 ± 0.007	0.845HisAsp: 0.845 ± 0.012	0.977HisGlu: 0.977 ± 0.011	0.788HisPhe: 0.788 ± 0.011	0.79HisGly: 0.79 ± 0.011	0.57HisHis: 0.57 ± 0.014	1.381HisIle: 1.381 ± 0.014	1.206HisLys: 1.206 ± 0.013	1.458HisLeu: 1.458 ± 0.015	0.31HisMet: 0.31 ± 0.006	1.286HisAsn: 1.286 ± 0.015	0.792HisPro: 0.792 ± 0.011	0.63HisGln: 0.63 ± 0.009	0.616HisArg: 0.616 ± 0.009	1.515HisSer: 1.515 ± 0.017	0.737HisThr: 0.737 ± 0.009	0.683HisVal: 0.683 ± 0.009	0.174HisTrp: 0.174 ± 0.005	0.848HisTyr: 0.848 ± 0.01	0.0HisXaa: 0.0 ± 0.0
Ile	3.01IleAla: 3.01 ± 0.022	1.301IleCys: 1.301 ± 0.014	5.157IleAsp: 5.157 ± 0.032	6.157IleGlu: 6.157 ± 0.046	4.01IlePhe: 4.01 ± 0.032	3.242IleGly: 3.242 ± 0.027	1.472IleHis: 1.472 ± 0.016	7.93IleIle: 7.93 ± 0.051	8.01IleLys: 8.01 ± 0.046	7.139IleLeu: 7.139 ± 0.042	1.598IleMet: 1.598 ± 0.014	8.951IleAsn: 8.951 ± 0.057	3.653IlePro: 3.653 ± 0.025	2.855IleGln: 2.855 ± 0.023	2.634IleArg: 2.634 ± 0.021	7.178IleSer: 7.178 ± 0.037	4.473IleThr: 4.473 ± 0.025	3.79IleVal: 3.79 ± 0.025	0.613IleTrp: 0.613 ± 0.01	3.889IleTyr: 3.889 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.29LysAla: 3.29 ± 0.025	1.396LysCys: 1.396 ± 0.022	5.136LysAsp: 5.136 ± 0.03	8.307LysGlu: 8.307 ± 0.068	3.338LysPhe: 3.338 ± 0.021	3.114LysGly: 3.114 ± 0.023	1.383LysHis: 1.383 ± 0.015	7.369LysIle: 7.369 ± 0.04	9.704LysLys: 9.704 ± 0.06	7.095LysLeu: 7.095 ± 0.038	1.786LysMet: 1.786 ± 0.017	8.815LysAsn: 8.815 ± 0.053	2.582LysPro: 2.582 ± 0.022	2.733LysGln: 2.733 ± 0.02	3.398LysArg: 3.398 ± 0.025	6.818LysSer: 6.818 ± 0.037	4.583LysThr: 4.583 ± 0.04	3.758LysVal: 3.758 ± 0.025	0.661LysTrp: 0.661 ± 0.008	4.454LysTyr: 4.454 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	3.126LeuAla: 3.126 ± 0.023	1.314LeuCys: 1.314 ± 0.012	4.1LeuAsp: 4.1 ± 0.022	5.304LeuGlu: 5.304 ± 0.035	4.002LeuPhe: 4.002 ± 0.031	3.11LeuGly: 3.11 ± 0.025	1.3LeuHis: 1.3 ± 0.013	7.003LeuIle: 7.003 ± 0.041	7.698LeuLys: 7.698 ± 0.043	7.395LeuLeu: 7.395 ± 0.05	1.735LeuMet: 1.735 ± 0.017	7.384LeuAsn: 7.384 ± 0.036	3.172LeuPro: 3.172 ± 0.029	2.571LeuGln: 2.571 ± 0.023	2.77LeuArg: 2.77 ± 0.021	7.025LeuSer: 7.025 ± 0.039	4.098LeuThr: 4.098 ± 0.024	3.547LeuVal: 3.547 ± 0.025	0.636LeuTrp: 0.636 ± 0.008	3.618LeuTyr: 3.618 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	0.955MetAla: 0.955 ± 0.012	0.206MetCys: 0.206 ± 0.004	1.356MetAsp: 1.356 ± 0.013	1.614MetGlu: 1.614 ± 0.014	0.738MetPhe: 0.738 ± 0.013	0.882MetGly: 0.882 ± 0.015	0.244MetHis: 0.244 ± 0.005	1.595MetIle: 1.595 ± 0.017	2.073MetLys: 2.073 ± 0.017	1.308MetLeu: 1.308 ± 0.014	0.497MetMet: 0.497 ± 0.009	2.326MetAsn: 2.326 ± 0.04	0.604MetPro: 0.604 ± 0.011	0.502MetGln: 0.502 ± 0.009	0.598MetArg: 0.598 ± 0.009	1.437MetSer: 1.437 ± 0.015	1.052MetThr: 1.052 ± 0.012	0.972MetVal: 0.972 ± 0.012	0.13MetTrp: 0.13 ± 0.004	0.744MetTyr: 0.744 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.044AsnAla: 3.044 ± 0.027	1.099AsnCys: 1.099 ± 0.015	6.119AsnAsp: 6.119 ± 0.042	7.763AsnGlu: 7.763 ± 0.058	3.358AsnPhe: 3.358 ± 0.029	4.202AsnGly: 4.202 ± 0.035	1.534AsnHis: 1.534 ± 0.014	8.723AsnIle: 8.723 ± 0.049	8.707AsnLys: 8.707 ± 0.058	6.483AsnLeu: 6.483 ± 0.043	1.948AsnMet: 1.948 ± 0.031	15.331AsnAsn: 15.331 ± 0.163	2.806AsnPro: 2.806 ± 0.02	3.213AsnGln: 3.213 ± 0.03	2.44AsnArg: 2.44 ± 0.019	9.048AsnSer: 9.048 ± 0.058	5.07AsnThr: 5.07 ± 0.039	3.844AsnVal: 3.844 ± 0.022	0.566AsnTrp: 0.566 ± 0.009	4.285AsnTyr: 4.285 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	1.326ProAla: 1.326 ± 0.021	0.414ProCys: 0.414 ± 0.009	1.641ProAsp: 1.641 ± 0.015	2.592ProGlu: 2.592 ± 0.02	1.53ProPhe: 1.53 ± 0.017	1.382ProGly: 1.382 ± 0.019	0.474ProHis: 0.474 ± 0.008	2.866ProIle: 2.866 ± 0.025	2.803ProLys: 2.803 ± 0.022	2.992ProLeu: 2.992 ± 0.024	0.681ProMet: 0.681 ± 0.012	2.732ProAsn: 2.732 ± 0.026	1.861ProPro: 1.861 ± 0.044	1.257ProGln: 1.257 ± 0.02	0.947ProArg: 0.947 ± 0.015	3.292ProSer: 3.292 ± 0.032	2.241ProThr: 2.241 ± 0.023	1.882ProVal: 1.882 ± 0.027	0.266ProTrp: 0.266 ± 0.006	1.474ProTyr: 1.474 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	1.275GlnAla: 1.275 ± 0.014	0.507GlnCys: 0.507 ± 0.01	1.402GlnAsp: 1.402 ± 0.014	2.234GlnGlu: 2.234 ± 0.022	1.395GlnPhe: 1.395 ± 0.014	1.219GlnGly: 1.219 ± 0.016	0.527GlnHis: 0.527 ± 0.009	2.482GlnIle: 2.482 ± 0.018	2.853GlnLys: 2.853 ± 0.02	2.807GlnLeu: 2.807 ± 0.025	0.724GlnMet: 0.724 ± 0.01	3.099GlnAsn: 3.099 ± 0.031	1.238GlnPro: 1.238 ± 0.024	2.049GlnGln: 2.049 ± 0.057	1.216GlnArg: 1.216 ± 0.013	2.543GlnSer: 2.543 ± 0.024	1.592GlnThr: 1.592 ± 0.017	1.325GlnVal: 1.325 ± 0.013	0.353GlnTrp: 0.353 ± 0.008	1.577GlnTyr: 1.577 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	1.234ArgAla: 1.234 ± 0.014	0.517ArgCys: 0.517 ± 0.009	1.685ArgAsp: 1.685 ± 0.023	2.177ArgGlu: 2.177 ± 0.022	1.319ArgPhe: 1.319 ± 0.014	1.303ArgGly: 1.303 ± 0.016	0.624ArgHis: 0.624 ± 0.011	2.621ArgIle: 2.621 ± 0.018	3.265ArgLys: 3.265 ± 0.024	2.497ArgLeu: 2.497 ± 0.024	0.673ArgMet: 0.673 ± 0.011	2.942ArgAsn: 2.942 ± 0.021	1.016ArgPro: 1.016 ± 0.016	1.01ArgGln: 1.01 ± 0.012	1.758ArgArg: 1.758 ± 0.023	2.591ArgSer: 2.591 ± 0.031	1.584ArgThr: 1.584 ± 0.015	1.486ArgVal: 1.486 ± 0.018	0.268ArgTrp: 0.268 ± 0.006	1.461ArgTyr: 1.461 ± 0.013	0.0ArgXaa: 0.0 ± 0.0
Ser	2.701SerAla: 2.701 ± 0.025	1.095SerCys: 1.095 ± 0.014	4.335SerAsp: 4.335 ± 0.029	5.136SerGlu: 5.136 ± 0.028	3.879SerPhe: 3.879 ± 0.029	3.468SerGly: 3.468 ± 0.029	1.366SerHis: 1.366 ± 0.016	7.246SerIle: 7.246 ± 0.034	7.121SerLys: 7.121 ± 0.038	7.248SerLeu: 7.248 ± 0.047	1.526SerMet: 1.526 ± 0.015	8.706SerAsn: 8.706 ± 0.062	3.161SerPro: 3.161 ± 0.033	2.775SerGln: 2.775 ± 0.023	2.547SerArg: 2.547 ± 0.03	9.959SerSer: 9.959 ± 0.077	5.197SerThr: 5.197 ± 0.042	3.671SerVal: 3.671 ± 0.022	0.579SerTrp: 0.579 ± 0.009	3.482SerTyr: 3.482 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	2.142ThrAla: 2.142 ± 0.023	0.792ThrCys: 0.792 ± 0.014	2.669ThrAsp: 2.669 ± 0.03	3.449ThrGlu: 3.449 ± 0.027	2.36ThrPhe: 2.36 ± 0.019	2.416ThrGly: 2.416 ± 0.027	0.804ThrHis: 0.804 ± 0.01	4.637ThrIle: 4.637 ± 0.03	4.326ThrLys: 4.326 ± 0.037	4.549ThrLeu: 4.549 ± 0.028	0.895ThrMet: 0.895 ± 0.01	4.78ThrAsn: 4.78 ± 0.031	2.403ThrPro: 2.403 ± 0.025	1.523ThrGln: 1.523 ± 0.016	1.474ThrArg: 1.474 ± 0.016	5.08ThrSer: 5.08 ± 0.038	4.84ThrThr: 4.84 ± 0.073	2.661ThrVal: 2.661 ± 0.024	0.418ThrTrp: 0.418 ± 0.007	2.136ThrTyr: 2.136 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	2.059ValAla: 2.059 ± 0.024	0.732ValCys: 0.732 ± 0.011	2.8ValAsp: 2.8 ± 0.021	3.22ValGlu: 3.22 ± 0.033	2.038ValPhe: 2.038 ± 0.018	2.069ValGly: 2.069 ± 0.023	0.782ValHis: 0.782 ± 0.009	3.853ValIle: 3.853 ± 0.027	3.938ValLys: 3.938 ± 0.025	3.835ValLeu: 3.835 ± 0.026	0.92ValMet: 0.92 ± 0.012	3.832ValAsn: 3.832 ± 0.022	1.791ValPro: 1.791 ± 0.019	1.459ValGln: 1.459 ± 0.014	1.368ValArg: 1.368 ± 0.014	3.511ValSer: 3.511 ± 0.025	2.57ValThr: 2.57 ± 0.021	2.599ValVal: 2.599 ± 0.027	0.356ValTrp: 0.356 ± 0.008	1.938ValTyr: 1.938 ± 0.016	0.0ValXaa: 0.0 ± 0.0
Trp	0.357TrpAla: 0.357 ± 0.007	0.23TrpCys: 0.23 ± 0.007	0.528TrpAsp: 0.528 ± 0.008	0.484TrpGlu: 0.484 ± 0.009	0.327TrpPhe: 0.327 ± 0.006	0.474TrpGly: 0.474 ± 0.011	0.152TrpHis: 0.152 ± 0.005	0.655TrpIle: 0.655 ± 0.01	0.701TrpLys: 0.701 ± 0.009	0.604TrpLeu: 0.604 ± 0.008	0.184TrpMet: 0.184 ± 0.005	0.707TrpAsn: 0.707 ± 0.01	0.185TrpPro: 0.185 ± 0.005	0.183TrpGln: 0.183 ± 0.005	0.285TrpArg: 0.285 ± 0.006	0.562TrpSer: 0.562 ± 0.011	0.389TrpThr: 0.389 ± 0.007	0.385TrpVal: 0.385 ± 0.008	0.109TrpTrp: 0.109 ± 0.005	0.38TrpTyr: 0.38 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.641TyrAla: 1.641 ± 0.017	0.897TyrCys: 0.897 ± 0.013	2.808TyrAsp: 2.808 ± 0.02	2.783TyrGlu: 2.783 ± 0.023	2.15TyrPhe: 2.15 ± 0.021	2.242TyrGly: 2.242 ± 0.022	0.939TyrHis: 0.939 ± 0.011	3.944TyrIle: 3.944 ± 0.027	3.468TyrLys: 3.468 ± 0.025	3.971TyrLeu: 3.971 ± 0.029	0.895TyrMet: 0.895 ± 0.012	4.132TyrAsn: 4.132 ± 0.03	1.553TyrPro: 1.553 ± 0.018	1.623TyrGln: 1.623 ± 0.016	1.46TyrArg: 1.46 ± 0.011	3.843TyrSer: 3.843 ± 0.026	2.126TyrThr: 2.126 ± 0.017	1.937TyrVal: 1.937 ± 0.02	0.361TyrTrp: 0.361 ± 0.007	2.792TyrTyr: 2.792 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 19964 proteins (9707863 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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