Streptococcus phage Javan187

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A321|A0A4D6A321_9CAUD Uncharacterized protein OS=Streptococcus phage Javan187 OX=2548035 GN=Javan187_0034 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84KK3 pKa = 7.98TDD5 pKa = 3.7AQEE8 pKa = 3.54KK9 pKa = 10.18AYY11 pKa = 10.61FGSDD15 pKa = 4.03EE16 pKa = 3.96IWAGQIPDD24 pKa = 3.99LDD26 pKa = 4.22EE27 pKa = 4.31EE28 pKa = 4.47VLIYY32 pKa = 10.76QNGHH36 pKa = 5.84YY37 pKa = 10.52DD38 pKa = 3.88VDD40 pKa = 4.12CMTDD44 pKa = 3.36VNSGIALYY52 pKa = 11.14NNDD55 pKa = 3.3YY56 pKa = 11.54DD57 pKa = 4.45DD58 pKa = 6.11FYY60 pKa = 10.7WCEE63 pKa = 3.84LEE65 pKa = 4.84APNTRR70 pKa = 11.84SGEE73 pKa = 4.12KK74 pKa = 10.26

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A3C6|A0A4D6A3C6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan187 OX=2548035 GN=Javan187_0033 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 10.51LRR4 pKa = 11.84IKK6 pKa = 10.02YY7 pKa = 9.48LRR9 pKa = 11.84QALGLTQSAFAAKK22 pKa = 10.1AQVNKK27 pKa = 10.73SLIANYY33 pKa = 9.63EE34 pKa = 4.13SQNPNPSLKK43 pKa = 10.29HH44 pKa = 5.56IEE46 pKa = 4.4KK47 pKa = 10.33IALALMM53 pKa = 4.66

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

7847

37

1045

186.8

21.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.945 ± 0.634

0.573 ± 0.132

6.652 ± 0.473

7.633 ± 0.404

4.129 ± 0.29

6.079 ± 0.369

1.567 ± 0.197

7.761 ± 0.305

9.01 ± 0.363

8.602 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.177

5.747 ± 0.35

2.625 ± 0.234

3.632 ± 0.25

3.861 ± 0.288

5.913 ± 0.498

5.939 ± 0.356

5.786 ± 0.255

1.083 ± 0.149

3.849 ± 0.428
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski