TM7 phylum sp. oral taxon 350

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Saccharibacteria

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A563C583|A0A563C583_9BACT Uncharacterized protein OS=TM7 phylum sp. oral taxon 350 OX=713052 GN=EUA76_02070 PE=4 SV=1
MM1 pKa = 7.63NMGEE5 pKa = 4.52KK6 pKa = 9.0EE7 pKa = 4.29TIKK10 pKa = 11.09DD11 pKa = 3.47EE12 pKa = 4.71FIKK15 pKa = 10.52VVKK18 pKa = 10.17EE19 pKa = 3.65IYY21 pKa = 10.42EE22 pKa = 3.98NATNCEE28 pKa = 4.16QFCSSLLVTTDD39 pKa = 3.19LPIYY43 pKa = 10.05KK44 pKa = 10.3DD45 pKa = 3.7LVCKK49 pKa = 10.55DD50 pKa = 3.74EE51 pKa = 4.48HH52 pKa = 6.95CSNLVEE58 pKa = 4.47LSRR61 pKa = 11.84SYY63 pKa = 11.11LYY65 pKa = 11.14SDD67 pKa = 4.71DD68 pKa = 4.24NKK70 pKa = 11.27EE71 pKa = 4.12NLNDD75 pKa = 3.93SLDD78 pKa = 3.86DD79 pKa = 3.75MVLGLL84 pKa = 4.74

Molecular weight:
9.67 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A563C5G8|A0A563C5G8_9BACT Zn_protease domain-containing protein (Fragment) OS=TM7 phylum sp. oral taxon 350 OX=713052 GN=EUA76_01900 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.07RR4 pKa = 11.84TFQPHH9 pKa = 5.23KK10 pKa = 8.46RR11 pKa = 11.84HH12 pKa = 5.96RR13 pKa = 11.84AKK15 pKa = 9.69THH17 pKa = 5.19GFRR20 pKa = 11.84ARR22 pKa = 11.84ISSKK26 pKa = 10.67AGQKK30 pKa = 8.09VLKK33 pKa = 9.85RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84IKK38 pKa = 10.73GRR40 pKa = 11.84AKK42 pKa = 10.5LAII45 pKa = 4.11

Molecular weight:
5.3 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

481

0

481

148586

38

1415

308.9

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.652 ± 0.119

0.614 ± 0.038

5.941 ± 0.084

6.56 ± 0.115

4.169 ± 0.083

5.984 ± 0.11

1.512 ± 0.043

8.405 ± 0.121

9.22 ± 0.138

9.697 ± 0.132

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.05

5.676 ± 0.091

3.265 ± 0.063

2.93 ± 0.073

4.187 ± 0.083

6.865 ± 0.088

5.354 ± 0.09

6.396 ± 0.105

0.772 ± 0.036

3.596 ± 0.082

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski