Glarea lozoyensis (strain ATCC 20868 / MF5171)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Helotiaceae; Glarea; Glarea lozoyensis

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13082 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S3D3B8|S3D3B8_GLAL2 Acetyl-CoA synthetase-like protein OS=Glarea lozoyensis (strain ATCC 20868 / MF5171) OX=1116229 GN=GLAREA_02479 PE=4 SV=1
MM1 pKa = 7.42SLHH4 pKa = 6.57LRR6 pKa = 11.84DD7 pKa = 3.22IDD9 pKa = 4.05SPKK12 pKa = 9.98EE13 pKa = 3.73SHH15 pKa = 6.6PFISTRR21 pKa = 11.84HH22 pKa = 4.43SHH24 pKa = 6.11NDD26 pKa = 3.17YY27 pKa = 10.94DD28 pKa = 4.17IKK30 pKa = 9.98TSNMDD35 pKa = 3.2SSDD38 pKa = 3.13ITDD41 pKa = 3.98PNMDD45 pKa = 4.76DD46 pKa = 3.7SDD48 pKa = 4.58NDD50 pKa = 3.32ISDD53 pKa = 4.14TEE55 pKa = 4.29TSDD58 pKa = 3.91VDD60 pKa = 4.33SPDD63 pKa = 3.69NQSPNYY69 pKa = 9.68DD70 pKa = 3.82SPNTDD75 pKa = 4.53DD76 pKa = 5.77DD77 pKa = 6.14DD78 pKa = 6.81SDD80 pKa = 5.13FDD82 pKa = 5.84DD83 pKa = 4.12SDD85 pKa = 4.78FYY87 pKa = 11.61LSSDD91 pKa = 5.06DD92 pKa = 5.62SDD94 pKa = 6.24DD95 pKa = 5.86DD96 pKa = 5.91DD97 pKa = 5.71FDD99 pKa = 4.43PSNYY103 pKa = 9.93EE104 pKa = 3.93SATHH108 pKa = 5.38YY109 pKa = 10.9ARR111 pKa = 11.84IYY113 pKa = 8.57EE114 pKa = 4.12FCEE117 pKa = 4.18AEE119 pKa = 4.2GVDD122 pKa = 5.36IEE124 pKa = 5.09DD125 pKa = 3.79LDD127 pKa = 3.55GRR129 pKa = 11.84YY130 pKa = 10.22YY131 pKa = 10.21IVDD134 pKa = 3.54HH135 pKa = 6.72EE136 pKa = 5.28SNGLEE141 pKa = 4.11SDD143 pKa = 3.77GAEE146 pKa = 4.22PDD148 pKa = 3.21HH149 pKa = 6.45STTEE153 pKa = 4.25TFSTLAEE160 pKa = 3.65WAAAEE165 pKa = 4.2KK166 pKa = 10.69LAVLYY171 pKa = 10.29SYY173 pKa = 11.5SLIPGEE179 pKa = 4.04KK180 pKa = 9.64PIVKK184 pKa = 9.43WIFPVEE190 pKa = 4.02YY191 pKa = 9.59RR192 pKa = 11.84KK193 pKa = 7.97TPPPPTAIALFLTDD207 pKa = 5.33LKK209 pKa = 11.34YY210 pKa = 10.98LLSWYY215 pKa = 9.83FISHH219 pKa = 7.86LIQSLVIMLQFGGILVILYY238 pKa = 9.5IVVGCGVAVWKK249 pKa = 10.77LGVLTWSTVCNGVSILIKK267 pKa = 10.67GVGEE271 pKa = 4.08FVNN274 pKa = 4.15

Molecular weight: 30.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S3DCB5|S3DCB5_GLAL2 RNA-binding RBD OS=Glarea lozoyensis (strain ATCC 20868 / MF5171) OX=1116229 GN=GLAREA_08134 PE=4 SV=1
MM1 pKa = 7.41SHH3 pKa = 6.25EE4 pKa = 4.71SVWNSRR10 pKa = 11.84PRR12 pKa = 11.84TYY14 pKa = 11.23GKK16 pKa = 9.94GARR19 pKa = 11.84ACRR22 pKa = 11.84VCTHH26 pKa = 6.71KK27 pKa = 11.0AGLIRR32 pKa = 11.84KK33 pKa = 8.76YY34 pKa = 10.73GLNICRR40 pKa = 11.84QCFRR44 pKa = 11.84EE45 pKa = 3.97KK46 pKa = 10.6AADD49 pKa = 3.13IGFVKK54 pKa = 10.38HH55 pKa = 5.95RR56 pKa = 4.18

Molecular weight: 6.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13082

0

13082

6275584

49

7890

479.7

53.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.07 ± 0.016

1.237 ± 0.008

5.547 ± 0.014

6.379 ± 0.019

3.771 ± 0.013

6.869 ± 0.02

2.277 ± 0.008

5.202 ± 0.016

5.329 ± 0.018

8.682 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.008

3.994 ± 0.012

5.863 ± 0.024

3.871 ± 0.016

5.742 ± 0.018

8.486 ± 0.025

6.201 ± 0.016

6.062 ± 0.013

1.445 ± 0.008

2.786 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski