Ramazzottius varieornatus (Water bear) (Tardigrade)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Tardigrada; Eutardigrada; Parachela; Hypsibioidea; Ramazzottiidae; Ramazzottius

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22940 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D1WAB2|A0A1D1WAB2_RAMVA Alpha-1 4 glucan phosphorylase OS=Ramazzottius varieornatus OX=947166 GN=RvY_17900 PE=3 SV=1
MM1 pKa = 7.8EE2 pKa = 6.23DD3 pKa = 5.29DD4 pKa = 5.33EE5 pKa = 5.4IFDD8 pKa = 3.89YY9 pKa = 11.18EE10 pKa = 5.42KK11 pKa = 11.27YY12 pKa = 10.29FADD15 pKa = 4.46KK16 pKa = 9.8WPQYY20 pKa = 11.41ANQQPAAMNPLTPSSAFAPDD40 pKa = 4.0NPPSTSGQQDD50 pKa = 3.86TVSSTFKK57 pKa = 10.46MEE59 pKa = 3.63GATAVAISYY68 pKa = 8.13PCLEE72 pKa = 4.23TWTDD76 pKa = 3.51GSPTYY81 pKa = 11.09VPFSPTYY88 pKa = 10.94SPDD91 pKa = 3.52NADD94 pKa = 3.5GGMDD98 pKa = 3.55MSEE101 pKa = 4.38TPPDD105 pKa = 3.41PHH107 pKa = 7.53LVKK110 pKa = 10.6EE111 pKa = 4.29LLVPGYY117 pKa = 7.63DD118 pKa = 3.43TPYY121 pKa = 11.2SPGNDD126 pKa = 3.27NEE128 pKa = 4.62KK129 pKa = 10.52DD130 pKa = 3.63GKK132 pKa = 10.72DD133 pKa = 3.44MEE135 pKa = 4.63EE136 pKa = 4.73GADD139 pKa = 3.55EE140 pKa = 4.18GHH142 pKa = 7.22PEE144 pKa = 4.1AEE146 pKa = 4.27AKK148 pKa = 10.58ADD150 pKa = 3.91LSSDD154 pKa = 3.13ALAIAVANALADD166 pKa = 3.85GVLGDD171 pKa = 3.8NN172 pKa = 4.62

Molecular weight: 18.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D1VLZ0|A0A1D1VLZ0_RAMVA Uncharacterized protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_12579 PE=4 SV=1
MM1 pKa = 7.43SLTVKK6 pKa = 10.15IQVNLRR12 pKa = 11.84VNLQANLRR20 pKa = 11.84ANLRR24 pKa = 11.84VNLRR28 pKa = 11.84VNLQVNRR35 pKa = 11.84QVNRR39 pKa = 11.84QVNRR43 pKa = 11.84QVNRR47 pKa = 11.84QVNRR51 pKa = 11.84QVNRR55 pKa = 11.84QVNRR59 pKa = 11.84QVNRR63 pKa = 11.84QVNRR67 pKa = 11.84QVRR70 pKa = 3.5

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22940

0

22940

8105517

12

11123

353.3

39.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.387 ± 0.015

1.833 ± 0.015

5.389 ± 0.014

6.083 ± 0.031

4.093 ± 0.012

6.03 ± 0.024

2.415 ± 0.009

4.718 ± 0.014

5.495 ± 0.023

9.03 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.007

4.068 ± 0.013

5.54 ± 0.019

4.222 ± 0.015

6.102 ± 0.015

8.425 ± 0.025

6.053 ± 0.027

6.621 ± 0.017

1.209 ± 0.005

2.865 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski