Methanosarcinales archaeon

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanosarcinales; unclassified Methanosarcinales

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1193 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482R572|A0A482R572_9EURY Uncharacterized protein OS=Methanosarcinales archaeon OX=2250255 GN=EOO65_03780 PE=4 SV=1
MM1 pKa = 7.35LSSYY5 pKa = 11.08ACWSLSYY12 pKa = 9.73TVLSITPLAAKK23 pKa = 9.97CQTPTNLFTYY33 pKa = 10.4SKK35 pKa = 9.3TDD37 pKa = 2.76NRR39 pKa = 11.84LRR41 pKa = 11.84IGATPLRR48 pKa = 11.84AYY50 pKa = 10.58NCTRR54 pKa = 11.84GYY56 pKa = 9.3TVVLRR61 pKa = 11.84AYY63 pKa = 7.37RR64 pKa = 11.84TSYY67 pKa = 7.99PTNFTDD73 pKa = 5.24CRR75 pKa = 11.84IEE77 pKa = 4.58LAVQQTNEE85 pKa = 4.37PPSFLPGACAPRR97 pKa = 11.84SVPEE101 pKa = 3.88RR102 pKa = 11.84ADD104 pKa = 2.83IGTVVGGVVAATDD117 pKa = 4.07PNVGQHH123 pKa = 6.03LVWSIDD129 pKa = 3.27AGAVIYY135 pKa = 9.91PFDD138 pKa = 4.55IGPCTGIITVNDD150 pKa = 3.71GAINYY155 pKa = 7.35TRR157 pKa = 11.84NAVFTLPVRR166 pKa = 11.84VMDD169 pKa = 3.89VGLTPSMSALCSITINVIDD188 pKa = 4.51VNDD191 pKa = 4.38PPTILTTEE199 pKa = 4.3LSAFEE204 pKa = 4.14NSDD207 pKa = 3.19VGEE210 pKa = 4.14EE211 pKa = 3.93VGQIVVFDD219 pKa = 5.01PDD221 pKa = 3.45TAGANLTYY229 pKa = 10.85NWTLVDD235 pKa = 3.81SSDD238 pKa = 3.12AFAISRR244 pKa = 11.84SGMVTVKK251 pKa = 10.53RR252 pKa = 11.84DD253 pKa = 3.31ILDD256 pKa = 3.94FEE258 pKa = 4.74VKK260 pKa = 8.45PTYY263 pKa = 9.26TYY265 pKa = 10.84RR266 pKa = 11.84VGVFDD271 pKa = 4.38GDD273 pKa = 3.6NFEE276 pKa = 4.65EE277 pKa = 4.87GVVVITLMDD286 pKa = 4.69ANDD289 pKa = 4.52PPTVHH294 pKa = 7.08PGSFSVPEE302 pKa = 3.94MSPAGTLVSGGSITVTDD319 pKa = 3.59QDD321 pKa = 3.19GDD323 pKa = 3.7AVTFEE328 pKa = 4.41VPVVDD333 pKa = 3.99TFTISGGRR341 pKa = 11.84FYY343 pKa = 11.34VAAGVVLDD351 pKa = 4.24YY352 pKa = 8.0EE353 pKa = 4.66TTPSYY358 pKa = 11.17SVDD361 pKa = 3.72VIANDD366 pKa = 3.54ANEE369 pKa = 4.13ASTVASITIRR379 pKa = 11.84VIDD382 pKa = 3.87VNEE385 pKa = 3.89VPEE388 pKa = 4.17FDD390 pKa = 3.84EE391 pKa = 4.5ATGVAMSIEE400 pKa = 4.71EE401 pKa = 4.08DD402 pKa = 3.96TLPGDD407 pKa = 3.87ALGAPISASDD417 pKa = 3.84PDD419 pKa = 4.16ANTILTYY426 pKa = 10.48EE427 pKa = 4.25IVTVTPAGNNHH438 pKa = 5.45QFSIDD443 pKa = 3.4GLTGQLRR450 pKa = 11.84AKK452 pKa = 8.4STSYY456 pKa = 10.86FDD458 pKa = 3.78YY459 pKa = 10.59EE460 pKa = 4.48LGVRR464 pKa = 11.84TYY466 pKa = 9.44TLAVKK471 pKa = 9.17VTDD474 pKa = 3.58NGSPRR479 pKa = 11.84LSATTTVTVTVTNVNEE495 pKa = 4.11APAFSAASYY504 pKa = 7.67TWSVRR509 pKa = 11.84EE510 pKa = 3.97DD511 pKa = 3.81TVVGTVMGSVFATDD525 pKa = 3.76PEE527 pKa = 4.86GTAVTYY533 pKa = 10.48EE534 pKa = 4.09LTTPSSLVTMSASGAFSVATDD555 pKa = 3.54LL556 pKa = 5.15

Molecular weight: 58.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482R4R5|A0A482R4R5_9EURY Uncharacterized protein OS=Methanosarcinales archaeon OX=2250255 GN=EOO65_00195 PE=4 SV=1
MM1 pKa = 6.96VASATTSSAAAALARR16 pKa = 11.84WLRR19 pKa = 11.84TPRR22 pKa = 11.84SHH24 pKa = 7.39TPFLWGVHH32 pKa = 4.87VCVCLCARR40 pKa = 11.84VCIAWCAGAVVSASWDD56 pKa = 3.39ATVKK60 pKa = 10.28VWDD63 pKa = 4.13LRR65 pKa = 11.84SPSMAAPEE73 pKa = 4.35DD74 pKa = 3.76ASTRR78 pKa = 11.84AADD81 pKa = 3.47MTLALPEE88 pKa = 4.41RR89 pKa = 11.84AFSMSLCSDD98 pKa = 4.16YY99 pKa = 11.13IVLVATAGQHH109 pKa = 5.52LVAYY113 pKa = 8.89DD114 pKa = 3.7LRR116 pKa = 11.84KK117 pKa = 9.89GDD119 pKa = 3.33EE120 pKa = 4.26MLWKK124 pKa = 10.4RR125 pKa = 11.84EE126 pKa = 4.12STVKK130 pKa = 10.55FQLRR134 pKa = 11.84CIRR137 pKa = 11.84AFADD141 pKa = 3.46GKK143 pKa = 10.73GYY145 pKa = 10.51VVSSIDD151 pKa = 3.26GRR153 pKa = 11.84CGVEE157 pKa = 3.93YY158 pKa = 10.36MEE160 pKa = 4.91EE161 pKa = 4.11ADD163 pKa = 4.06GKK165 pKa = 9.55PFSFKK170 pKa = 9.97CHH172 pKa = 6.36RR173 pKa = 11.84SKK175 pKa = 10.05TPDD178 pKa = 3.1GKK180 pKa = 10.96EE181 pKa = 3.73LITPVNALAFNPVYY195 pKa = 10.14NTFVTGGGDD204 pKa = 3.48GTVAAWDD211 pKa = 3.79HH212 pKa = 5.58VSKK215 pKa = 10.89KK216 pKa = 10.19RR217 pKa = 11.84LAVLGTYY224 pKa = 7.32PTSIAALAFNRR235 pKa = 11.84CVQAGYY241 pKa = 10.82ASPARR246 pKa = 11.84PSARR250 pKa = 11.84ANAVVFTLPPRR261 pKa = 11.84VRR263 pKa = 11.84THH265 pKa = 5.23VLARR269 pKa = 11.84ACACVCVCVADD280 pKa = 4.42AGRR283 pKa = 11.84APSWRR288 pKa = 11.84LPRR291 pKa = 11.84RR292 pKa = 11.84TRR294 pKa = 11.84GRR296 pKa = 11.84RR297 pKa = 11.84VRR299 pKa = 11.84RR300 pKa = 11.84SIPRR304 pKa = 11.84TASMCTVWGAPWLARR319 pKa = 11.84ALRR322 pKa = 11.84KK323 pKa = 9.87VADD326 pKa = 3.8PAAMVVRR333 pKa = 11.84HH334 pKa = 5.67ASWRR338 pKa = 11.84RR339 pKa = 11.84CRR341 pKa = 11.84PSWRR345 pKa = 11.84ARR347 pKa = 11.84PRR349 pKa = 11.84VRR351 pKa = 11.84VVPLSLILRR360 pKa = 11.84TSLL363 pKa = 3.81

Molecular weight: 39.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1193

0

1193

315384

34

3471

264.4

28.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.02 ± 0.111

2.205 ± 0.051

4.771 ± 0.06

4.293 ± 0.083

3.099 ± 0.046

6.848 ± 0.109

2.824 ± 0.055

3.434 ± 0.05

2.571 ± 0.066

9.177 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.037

2.917 ± 0.052

5.626 ± 0.077

3.377 ± 0.047

6.582 ± 0.07

8.565 ± 0.113

6.651 ± 0.084

8.065 ± 0.075

1.356 ± 0.034

2.117 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski