Limnohabitans sp. JirII-31

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Limnohabitans; unclassified Limnohabitans

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M6VIW6|A0A2M6VIW6_9BURK Iron ABC transporter permease OS=Limnohabitans sp. JirII-31 OX=1977908 GN=B9Z41_07990 PE=3 SV=1
MM1 pKa = 7.65SDD3 pKa = 3.31GNGGHH8 pKa = 6.35ATALATINLAAVNDD22 pKa = 3.94APVTVGEE29 pKa = 4.54LSSTDD34 pKa = 3.24EE35 pKa = 4.29DD36 pKa = 3.68VGLTFTQAQLLNNDD50 pKa = 3.87SDD52 pKa = 3.88VDD54 pKa = 3.96TATDD58 pKa = 4.01GQVLTITAVSGATNGTVSMLANGDD82 pKa = 2.88IRR84 pKa = 11.84FIPNANYY91 pKa = 10.23HH92 pKa = 5.27GTASFTYY99 pKa = 9.31TVSDD103 pKa = 4.1GNGGTTDD110 pKa = 3.29ASVSITVNAVNDD122 pKa = 3.79VPVVTGEE129 pKa = 4.32TVSTNEE135 pKa = 4.34DD136 pKa = 3.17TTLWFIPSDD145 pKa = 3.84LLTNDD150 pKa = 3.08TDD152 pKa = 3.96IDD154 pKa = 4.11VATDD158 pKa = 3.79GQVLSITAVSNATHH172 pKa = 5.72GTVGFVTQADD182 pKa = 4.0GSRR185 pKa = 11.84RR186 pKa = 11.84IAFTPDD192 pKa = 2.51ANYY195 pKa = 10.51FGVASFQYY203 pKa = 9.51TVSDD207 pKa = 4.06GAGGTSTGTVSINVAQINDD226 pKa = 3.58VPVASDD232 pKa = 3.82DD233 pKa = 4.03SLSGSAEE240 pKa = 3.96DD241 pKa = 3.76NALHH245 pKa = 7.12ISFAKK250 pKa = 10.53LLSNDD255 pKa = 3.08TDD257 pKa = 3.94ADD259 pKa = 3.81STNALLGGTNDD270 pKa = 3.45VLTVSAVGNATHH282 pKa = 7.14GSVALVNGEE291 pKa = 4.15IVFTPDD297 pKa = 2.75ANYY300 pKa = 10.4HH301 pKa = 5.28GVASFVYY308 pKa = 8.87QVRR311 pKa = 11.84DD312 pKa = 3.23QSGALSQATAAFTITAVNDD331 pKa = 3.48APVATGEE338 pKa = 4.5TISSSEE344 pKa = 4.61DD345 pKa = 3.2SNLLLNQSDD354 pKa = 4.25LLKK357 pKa = 10.73NDD359 pKa = 3.69SDD361 pKa = 3.66VDD363 pKa = 3.67AATDD367 pKa = 4.04GQVLTIKK374 pKa = 10.4AVSNARR380 pKa = 11.84HH381 pKa = 5.79GSVTLNADD389 pKa = 3.3GTILFVPDD397 pKa = 3.26ANYY400 pKa = 11.03YY401 pKa = 8.07GTASFDD407 pKa = 3.58YY408 pKa = 10.64TVDD411 pKa = 3.84DD412 pKa = 4.87GNGGAASATATITLLSVNDD431 pKa = 3.86APVATGEE438 pKa = 4.36TVQDD442 pKa = 4.06VEE444 pKa = 4.44DD445 pKa = 3.66QQLTILSSALLQNDD459 pKa = 3.42TDD461 pKa = 3.63VDD463 pKa = 3.72NTQASLTISRR473 pKa = 11.84VLSGAGGTAYY483 pKa = 10.81LNASGHH489 pKa = 5.67VVFTPNTNYY498 pKa = 10.7NGNATFTYY506 pKa = 8.86WVKK509 pKa = 11.14DD510 pKa = 3.46PDD512 pKa = 4.06GLEE515 pKa = 4.38SNAVTATVVVSAVNDD530 pKa = 3.44APTAQGEE537 pKa = 4.64IVTGASEE544 pKa = 4.55DD545 pKa = 3.45AVFYY549 pKa = 10.69INKK552 pKa = 8.37STLLANDD559 pKa = 3.91SDD561 pKa = 4.31IDD563 pKa = 4.03DD564 pKa = 4.43ASSALSLSWVGNASGGSVSLDD585 pKa = 3.13GNGNVVFTPNANYY598 pKa = 10.45NGNASFQYY606 pKa = 10.14KK607 pKa = 9.64VRR609 pKa = 11.84DD610 pKa = 3.39AAGAEE615 pKa = 4.38SAVVQAVIPVTAVNDD630 pKa = 3.67APVAVDD636 pKa = 5.57DD637 pKa = 6.03KK638 pKa = 11.56FDD640 pKa = 3.68TYY642 pKa = 11.64KK643 pKa = 9.69NTPVTVAFNQLTSNDD658 pKa = 3.52SDD660 pKa = 3.78VDD662 pKa = 3.84GDD664 pKa = 4.29SLTVSAVRR672 pKa = 11.84DD673 pKa = 3.69HH674 pKa = 7.15ANGHH678 pKa = 5.74ASIVNGQVQFQATTGFTGTASFDD701 pKa = 3.62YY702 pKa = 10.96LSDD705 pKa = 4.67DD706 pKa = 3.56GHH708 pKa = 7.17GGQTWATAYY717 pKa = 10.87VNVKK721 pKa = 9.7VPPNQYY727 pKa = 8.55PTMTLKK733 pKa = 10.69YY734 pKa = 10.56FPVCDD739 pKa = 3.58GKK741 pKa = 11.16YY742 pKa = 10.44GYY744 pKa = 10.06DD745 pKa = 3.14ILGSTILTNGIQTFSLHH762 pKa = 7.48DD763 pKa = 4.9DD764 pKa = 3.98GSTSAISMRR773 pKa = 11.84FVSATFKK780 pKa = 10.83PPLVLTLL787 pKa = 3.95

Molecular weight: 80.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M6VJ29|A0A2M6VJ29_9BURK zf-CHCC domain-containing protein OS=Limnohabitans sp. JirII-31 OX=1977908 GN=B9Z41_08605 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3283

0

3283

1066846

33

6459

325.0

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.608 ± 0.051

0.977 ± 0.016

5.235 ± 0.039

4.949 ± 0.046

3.542 ± 0.033

7.886 ± 0.055

2.596 ± 0.03

4.454 ± 0.031

3.849 ± 0.047

10.565 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.026

3.092 ± 0.052

4.963 ± 0.036

4.616 ± 0.037

5.737 ± 0.043

5.828 ± 0.057

5.636 ± 0.072

7.956 ± 0.036

1.492 ± 0.022

2.31 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski