Amino acid dipepetide frequency for Limnohabitans sp. JirII-31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.462AlaAla: 13.462 ± 0.181
1.396AlaCys: 1.396 ± 0.044
5.83AlaAsp: 5.83 ± 0.089
5.692AlaGlu: 5.692 ± 0.086
3.771AlaPhe: 3.771 ± 0.058
8.525AlaGly: 8.525 ± 0.117
3.128AlaHis: 3.128 ± 0.066
4.929AlaIle: 4.929 ± 0.073
4.794AlaLys: 4.794 ± 0.084
13.769AlaLeu: 13.769 ± 0.134
3.525AlaMet: 3.525 ± 0.06
3.316AlaAsn: 3.316 ± 0.102
5.125AlaPro: 5.125 ± 0.081
6.769AlaGln: 6.769 ± 0.103
6.561AlaArg: 6.561 ± 0.099
6.896AlaSer: 6.896 ± 0.126
5.799AlaThr: 5.799 ± 0.107
8.414AlaVal: 8.414 ± 0.099
1.883AlaTrp: 1.883 ± 0.059
2.498AlaTyr: 2.498 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.141CysAla: 1.141 ± 0.038
0.124CysCys: 0.124 ± 0.01
0.585CysAsp: 0.585 ± 0.024
0.531CysGlu: 0.531 ± 0.026
0.301CysPhe: 0.301 ± 0.017
1.02CysGly: 1.02 ± 0.037
0.306CysHis: 0.306 ± 0.018
0.443CysIle: 0.443 ± 0.02
0.277CysLys: 0.277 ± 0.017
0.942CysLeu: 0.942 ± 0.031
0.247CysMet: 0.247 ± 0.017
0.263CysAsn: 0.263 ± 0.017
0.491CysPro: 0.491 ± 0.025
0.372CysGln: 0.372 ± 0.018
0.462CysArg: 0.462 ± 0.021
0.49CysSer: 0.49 ± 0.024
0.541CysThr: 0.541 ± 0.025
0.885CysVal: 0.885 ± 0.032
0.136CysTrp: 0.136 ± 0.012
0.209CysTyr: 0.209 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
6.577AspAla: 6.577 ± 0.093
0.468AspCys: 0.468 ± 0.022
2.614AspAsp: 2.614 ± 0.064
2.828AspGlu: 2.828 ± 0.063
2.095AspPhe: 2.095 ± 0.048
4.058AspGly: 4.058 ± 0.082
1.208AspHis: 1.208 ± 0.041
2.69AspIle: 2.69 ± 0.047
2.055AspLys: 2.055 ± 0.051
5.318AspLeu: 5.318 ± 0.08
1.531AspMet: 1.531 ± 0.044
1.444AspAsn: 1.444 ± 0.036
2.594AspPro: 2.594 ± 0.058
1.79AspGln: 1.79 ± 0.042
2.592AspArg: 2.592 ± 0.053
2.42AspSer: 2.42 ± 0.064
3.078AspThr: 3.078 ± 0.097
4.585AspVal: 4.585 ± 0.079
0.937AspTrp: 0.937 ± 0.034
1.468AspTyr: 1.468 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
6.042GluAla: 6.042 ± 0.094
0.352GluCys: 0.352 ± 0.02
2.088GluAsp: 2.088 ± 0.053
2.254GluGlu: 2.254 ± 0.052
1.814GluPhe: 1.814 ± 0.042
3.567GluGly: 3.567 ± 0.073
1.383GluHis: 1.383 ± 0.039
2.577GluIle: 2.577 ± 0.055
2.103GluLys: 2.103 ± 0.049
5.559GluLeu: 5.559 ± 0.095
1.378GluMet: 1.378 ± 0.039
1.279GluAsn: 1.279 ± 0.039
2.127GluPro: 2.127 ± 0.058
2.449GluGln: 2.449 ± 0.056
3.772GluArg: 3.772 ± 0.079
2.47GluSer: 2.47 ± 0.053
2.381GluThr: 2.381 ± 0.054
4.23GluVal: 4.23 ± 0.076
0.697GluTrp: 0.697 ± 0.027
0.967GluTyr: 0.967 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.994PheAla: 3.994 ± 0.067
0.424PheCys: 0.424 ± 0.019
2.516PheAsp: 2.516 ± 0.055
2.197PheGlu: 2.197 ± 0.047
1.422PhePhe: 1.422 ± 0.046
3.16PheGly: 3.16 ± 0.06
0.734PheHis: 0.734 ± 0.027
1.502PheIle: 1.502 ± 0.039
1.629PheLys: 1.629 ± 0.04
2.88PheLeu: 2.88 ± 0.063
0.922PheMet: 0.922 ± 0.033
1.334PheAsn: 1.334 ± 0.036
1.461PhePro: 1.461 ± 0.036
1.218PheGln: 1.218 ± 0.038
1.444PheArg: 1.444 ± 0.042
2.224PheSer: 2.224 ± 0.048
2.044PheThr: 2.044 ± 0.046
2.943PheVal: 2.943 ± 0.066
0.486PheTrp: 0.486 ± 0.023
0.885PheTyr: 0.885 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
7.857GlyAla: 7.857 ± 0.125
0.865GlyCys: 0.865 ± 0.031
3.748GlyAsp: 3.748 ± 0.072
3.874GlyGlu: 3.874 ± 0.061
3.14GlyPhe: 3.14 ± 0.049
6.337GlyGly: 6.337 ± 0.146
2.127GlyHis: 2.127 ± 0.044
3.77GlyIle: 3.77 ± 0.064
3.386GlyLys: 3.386 ± 0.075
8.849GlyLeu: 8.849 ± 0.117
2.239GlyMet: 2.239 ± 0.052
2.451GlyAsn: 2.451 ± 0.129
2.869GlyPro: 2.869 ± 0.053
3.837GlyGln: 3.837 ± 0.071
4.117GlyArg: 4.117 ± 0.069
4.623GlySer: 4.623 ± 0.135
4.477GlyThr: 4.477 ± 0.15
6.72GlyVal: 6.72 ± 0.1
1.295GlyTrp: 1.295 ± 0.041
2.277GlyTyr: 2.277 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
3.153HisAla: 3.153 ± 0.062
0.305HisCys: 0.305 ± 0.019
1.366HisAsp: 1.366 ± 0.041
1.168HisGlu: 1.168 ± 0.041
0.979HisPhe: 0.979 ± 0.031
2.138HisGly: 2.138 ± 0.053
0.805HisHis: 0.805 ± 0.033
1.301HisIle: 1.301 ± 0.038
0.785HisLys: 0.785 ± 0.027
2.616HisLeu: 2.616 ± 0.055
0.686HisMet: 0.686 ± 0.029
0.732HisAsn: 0.732 ± 0.026
1.547HisPro: 1.547 ± 0.046
0.979HisGln: 0.979 ± 0.03
1.38HisArg: 1.38 ± 0.037
1.274HisSer: 1.274 ± 0.04
1.623HisThr: 1.623 ± 0.043
1.959HisVal: 1.959 ± 0.048
0.479HisTrp: 0.479 ± 0.022
0.686HisTyr: 0.686 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.558IleAla: 5.558 ± 0.085
0.434IleCys: 0.434 ± 0.023
3.096IleAsp: 3.096 ± 0.05
2.968IleGlu: 2.968 ± 0.063
1.428IlePhe: 1.428 ± 0.038
3.873IleGly: 3.873 ± 0.069
1.038IleHis: 1.038 ± 0.029
1.646IleIle: 1.646 ± 0.044
1.851IleLys: 1.851 ± 0.041
3.248IleLeu: 3.248 ± 0.068
0.867IleMet: 0.867 ± 0.032
1.694IleAsn: 1.694 ± 0.051
2.109IlePro: 2.109 ± 0.051
1.791IleGln: 1.791 ± 0.039
2.449IleArg: 2.449 ± 0.05
2.735IleSer: 2.735 ± 0.065
2.959IleThr: 2.959 ± 0.069
3.215IleVal: 3.215 ± 0.061
0.516IleTrp: 0.516 ± 0.023
1.063IleTyr: 1.063 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
4.635LysAla: 4.635 ± 0.076
0.201LysCys: 0.201 ± 0.013
2.0LysAsp: 2.0 ± 0.049
1.789LysGlu: 1.789 ± 0.048
1.23LysPhe: 1.23 ± 0.042
2.705LysGly: 2.705 ± 0.056
0.876LysHis: 0.876 ± 0.032
1.839LysIle: 1.839 ± 0.05
1.832LysLys: 1.832 ± 0.057
4.041LysLeu: 4.041 ± 0.072
0.995LysMet: 0.995 ± 0.032
1.308LysAsn: 1.308 ± 0.038
2.416LysPro: 2.416 ± 0.057
1.52LysGln: 1.52 ± 0.042
2.389LysArg: 2.389 ± 0.051
2.228LysSer: 2.228 ± 0.056
2.375LysThr: 2.375 ± 0.053
2.965LysVal: 2.965 ± 0.064
0.376LysTrp: 0.376 ± 0.018
0.766LysTyr: 0.766 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
12.662LeuAla: 12.662 ± 0.142
1.122LeuCys: 1.122 ± 0.036
5.452LeuAsp: 5.452 ± 0.077
4.732LeuGlu: 4.732 ± 0.082
3.32LeuPhe: 3.32 ± 0.065
8.543LeuGly: 8.543 ± 0.12
2.698LeuHis: 2.698 ± 0.055
4.497LeuIle: 4.497 ± 0.067
4.362LeuLys: 4.362 ± 0.068
10.428LeuLeu: 10.428 ± 0.149
2.943LeuMet: 2.943 ± 0.06
3.61LeuAsn: 3.61 ± 0.077
5.745LeuPro: 5.745 ± 0.099
4.783LeuGln: 4.783 ± 0.078
6.48LeuArg: 6.48 ± 0.102
7.12LeuSer: 7.12 ± 0.091
5.852LeuThr: 5.852 ± 0.095
7.837LeuVal: 7.837 ± 0.098
1.434LeuTrp: 1.434 ± 0.044
2.03LeuTyr: 2.03 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
3.42MetAla: 3.42 ± 0.062
0.216MetCys: 0.216 ± 0.015
1.279MetAsp: 1.279 ± 0.031
1.027MetGlu: 1.027 ± 0.035
0.813MetPhe: 0.813 ± 0.029
2.266MetGly: 2.266 ± 0.048
0.685MetHis: 0.685 ± 0.026
0.978MetIle: 0.978 ± 0.032
1.149MetLys: 1.149 ± 0.031
2.685MetLeu: 2.685 ± 0.068
0.651MetMet: 0.651 ± 0.029
1.024MetAsn: 1.024 ± 0.03
1.549MetPro: 1.549 ± 0.041
1.294MetGln: 1.294 ± 0.037
1.741MetArg: 1.741 ± 0.041
1.828MetSer: 1.828 ± 0.04
1.769MetThr: 1.769 ± 0.043
2.036MetVal: 2.036 ± 0.05
0.255MetTrp: 0.255 ± 0.015
0.439MetTyr: 0.439 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.776AsnAla: 3.776 ± 0.108
0.262AsnCys: 0.262 ± 0.015
1.719AsnAsp: 1.719 ± 0.065
1.278AsnGlu: 1.278 ± 0.035
1.128AsnPhe: 1.128 ± 0.033
2.531AsnGly: 2.531 ± 0.095
0.676AsnHis: 0.676 ± 0.025
1.49AsnIle: 1.49 ± 0.041
1.159AsnLys: 1.159 ± 0.041
3.076AsnLeu: 3.076 ± 0.068
0.709AsnMet: 0.709 ± 0.028
1.1AsnAsn: 1.1 ± 0.061
2.024AsnPro: 2.024 ± 0.046
1.214AsnGln: 1.214 ± 0.045
1.65AsnArg: 1.65 ± 0.045
1.602AsnSer: 1.602 ± 0.087
2.003AsnThr: 2.003 ± 0.102
2.223AsnVal: 2.223 ± 0.074
0.477AsnTrp: 0.477 ± 0.021
0.827AsnTyr: 0.827 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
5.461ProAla: 5.461 ± 0.076
0.37ProCys: 0.37 ± 0.018
2.993ProAsp: 2.993 ± 0.054
3.293ProGlu: 3.293 ± 0.07
1.793ProPhe: 1.793 ± 0.045
3.834ProGly: 3.834 ± 0.067
1.38ProHis: 1.38 ± 0.041
2.078ProIle: 2.078 ± 0.05
1.878ProLys: 1.878 ± 0.047
4.807ProLeu: 4.807 ± 0.08
1.489ProMet: 1.489 ± 0.039
1.634ProAsn: 1.634 ± 0.042
2.04ProPro: 2.04 ± 0.055
2.316ProGln: 2.316 ± 0.053
2.306ProArg: 2.306 ± 0.056
2.999ProSer: 2.999 ± 0.062
2.885ProThr: 2.885 ± 0.057
4.025ProVal: 4.025 ± 0.068
0.775ProTrp: 0.775 ± 0.03
1.274ProTyr: 1.274 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
6.563GlnAla: 6.563 ± 0.09
0.373GlnCys: 0.373 ± 0.022
2.17GlnAsp: 2.17 ± 0.045
1.993GlnGlu: 1.993 ± 0.05
1.412GlnPhe: 1.412 ± 0.036
3.626GlnGly: 3.626 ± 0.063
1.429GlnHis: 1.429 ± 0.048
1.947GlnIle: 1.947 ± 0.044
1.373GlnLys: 1.373 ± 0.039
4.751GlnLeu: 4.751 ± 0.08
1.13GlnMet: 1.13 ± 0.035
1.071GlnAsn: 1.071 ± 0.035
2.251GlnPro: 2.251 ± 0.054
2.423GlnGln: 2.423 ± 0.06
3.492GlnArg: 3.492 ± 0.061
2.447GlnSer: 2.447 ± 0.05
2.521GlnThr: 2.521 ± 0.053
3.608GlnVal: 3.608 ± 0.065
0.752GlnTrp: 0.752 ± 0.028
0.832GlnTyr: 0.832 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
6.206ArgAla: 6.206 ± 0.081
0.587ArgCys: 0.587 ± 0.026
3.159ArgAsp: 3.159 ± 0.062
3.344ArgGlu: 3.344 ± 0.059
2.313ArgPhe: 2.313 ± 0.05
3.652ArgGly: 3.652 ± 0.068
1.646ArgHis: 1.646 ± 0.047
2.985ArgIle: 2.985 ± 0.053
1.903ArgLys: 1.903 ± 0.048
6.439ArgLeu: 6.439 ± 0.107
1.81ArgMet: 1.81 ± 0.047
1.676ArgAsn: 1.676 ± 0.038
2.418ArgPro: 2.418 ± 0.051
2.673ArgGln: 2.673 ± 0.058
3.344ArgArg: 3.344 ± 0.071
3.001ArgSer: 3.001 ± 0.066
2.774ArgThr: 2.774 ± 0.051
4.741ArgVal: 4.741 ± 0.071
1.048ArgTrp: 1.048 ± 0.033
1.647ArgTyr: 1.647 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
6.69SerAla: 6.69 ± 0.109
0.504SerCys: 0.504 ± 0.021
2.973SerAsp: 2.973 ± 0.074
2.581SerGlu: 2.581 ± 0.054
2.198SerPhe: 2.198 ± 0.047
5.378SerGly: 5.378 ± 0.156
1.479SerHis: 1.479 ± 0.04
2.502SerIle: 2.502 ± 0.058
1.974SerLys: 1.974 ± 0.049
6.211SerLeu: 6.211 ± 0.09
1.522SerMet: 1.522 ± 0.04
1.825SerAsn: 1.825 ± 0.087
3.032SerPro: 3.032 ± 0.064
2.38SerGln: 2.38 ± 0.048
2.988SerArg: 2.988 ± 0.049
3.685SerSer: 3.685 ± 0.115
3.388SerThr: 3.388 ± 0.116
4.608SerVal: 4.608 ± 0.084
0.83SerTrp: 0.83 ± 0.027
1.528SerTyr: 1.528 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
5.933ThrAla: 5.933 ± 0.107
0.485ThrCys: 0.485 ± 0.024
2.81ThrAsp: 2.81 ± 0.072
2.521ThrGlu: 2.521 ± 0.053
1.921ThrPhe: 1.921 ± 0.044
4.705ThrGly: 4.705 ± 0.15
1.595ThrHis: 1.595 ± 0.041
2.309ThrIle: 2.309 ± 0.077
1.574ThrLys: 1.574 ± 0.043
6.759ThrLeu: 6.759 ± 0.118
1.151ThrMet: 1.151 ± 0.034
1.503ThrAsn: 1.503 ± 0.078
3.99ThrPro: 3.99 ± 0.069
2.922ThrGln: 2.922 ± 0.059
3.097ThrArg: 3.097 ± 0.053
3.276ThrSer: 3.276 ± 0.094
3.309ThrThr: 3.309 ± 0.11
4.452ThrVal: 4.452 ± 0.109
0.778ThrTrp: 0.778 ± 0.025
1.268ThrTyr: 1.268 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
8.929ValAla: 8.929 ± 0.116
0.837ValCys: 0.837 ± 0.029
4.109ValAsp: 4.109 ± 0.071
3.74ValGlu: 3.74 ± 0.07
2.816ValPhe: 2.816 ± 0.057
5.822ValGly: 5.822 ± 0.097
1.845ValHis: 1.845 ± 0.045
3.596ValIle: 3.596 ± 0.069
3.055ValLys: 3.055 ± 0.064
8.849ValLeu: 8.849 ± 0.112
2.361ValMet: 2.361 ± 0.049
2.518ValAsn: 2.518 ± 0.092
3.94ValPro: 3.94 ± 0.066
3.512ValGln: 3.512 ± 0.07
4.562ValArg: 4.562 ± 0.079
4.81ValSer: 4.81 ± 0.094
4.414ValThr: 4.414 ± 0.104
7.099ValVal: 7.099 ± 0.093
1.136ValTrp: 1.136 ± 0.041
1.608ValTyr: 1.608 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.41TrpAla: 1.41 ± 0.043
0.198TrpCys: 0.198 ± 0.011
0.62TrpAsp: 0.62 ± 0.028
0.511TrpGlu: 0.511 ± 0.023
0.558TrpPhe: 0.558 ± 0.025
1.087TrpGly: 1.087 ± 0.036
0.437TrpHis: 0.437 ± 0.021
0.598TrpIle: 0.598 ± 0.026
0.407TrpLys: 0.407 ± 0.021
2.111TrpLeu: 2.111 ± 0.051
0.481TrpMet: 0.481 ± 0.024
0.401TrpAsn: 0.401 ± 0.021
0.723TrpPro: 0.723 ± 0.025
0.918TrpGln: 0.918 ± 0.028
1.111TrpArg: 1.111 ± 0.04
0.802TrpSer: 0.802 ± 0.03
0.729TrpThr: 0.729 ± 0.029
1.251TrpVal: 1.251 ± 0.037
0.279TrpTrp: 0.279 ± 0.019
0.292TrpTyr: 0.292 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.614TyrAla: 2.614 ± 0.062
0.246TyrCys: 0.246 ± 0.015
1.223TyrAsp: 1.223 ± 0.034
1.171TyrGlu: 1.171 ± 0.037
0.969TyrPhe: 0.969 ± 0.031
2.03TyrGly: 2.03 ± 0.048
0.487TyrHis: 0.487 ± 0.023
0.861TyrIle: 0.861 ± 0.032
0.94TyrLys: 0.94 ± 0.029
2.314TyrLeu: 2.314 ± 0.043
0.463TyrMet: 0.463 ± 0.021
0.738TyrAsn: 0.738 ± 0.032
1.178TyrPro: 1.178 ± 0.036
1.004TyrGln: 1.004 ± 0.036
1.431TyrArg: 1.431 ± 0.041
1.347TyrSer: 1.347 ± 0.034
1.436TyrThr: 1.436 ± 0.049
1.759TyrVal: 1.759 ± 0.046
0.351TyrTrp: 0.351 ± 0.019
0.536TyrTyr: 0.536 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3283 proteins (1066846 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski