Groundnut rosette virus (strain MC1) (GRV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus; Groundnut rosette virus

Average proteome isoelectric point is 7.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q67683-2|RDRP-2_GRVMC Isoform of Q67683 Isoform ORF1 protein of RNA-directed RNA polymerase OS=Groundnut rosette virus (strain MC1) OX=1005060 GN=ORF1-ORF2 PE=4 SV=2
MM1 pKa = 7.04ATIRR5 pKa = 11.84HH6 pKa = 5.28IMDD9 pKa = 3.88WLRR12 pKa = 11.84PTFTPLAGVKK22 pKa = 9.61SRR24 pKa = 11.84QEE26 pKa = 4.29CIAHH30 pKa = 6.5YY31 pKa = 10.68GDD33 pKa = 4.92DD34 pKa = 3.84WALMITQSRR43 pKa = 11.84MTLSAEE49 pKa = 3.96AQVNAWYY56 pKa = 10.21EE57 pKa = 3.93GGAEE61 pKa = 4.28VNGSIPSVEE70 pKa = 4.2GNAAAPQAAAVVAEE84 pKa = 4.78PPHH87 pKa = 6.51PSPAEE92 pKa = 3.93NEE94 pKa = 4.37GEE96 pKa = 4.12DD97 pKa = 4.09ASWVAALPAPPTYY110 pKa = 10.48EE111 pKa = 4.2VVQGRR116 pKa = 11.84APTLEE121 pKa = 4.6EE122 pKa = 3.32IHH124 pKa = 6.19GASRR128 pKa = 11.84LQIVPYY134 pKa = 8.91TGRR137 pKa = 11.84ARR139 pKa = 11.84VIGEE143 pKa = 4.35DD144 pKa = 3.51EE145 pKa = 4.34VLPSPVLSSIWRR157 pKa = 11.84ATRR160 pKa = 11.84CPGRR164 pKa = 11.84ILRR167 pKa = 11.84VLGTLLKK174 pKa = 10.56PGACQRR180 pKa = 11.84HH181 pKa = 5.67LEE183 pKa = 4.31DD184 pKa = 4.17LRR186 pKa = 11.84EE187 pKa = 4.02VQPQVCVGNPCEE199 pKa = 4.45VKK201 pKa = 10.47LQEE204 pKa = 4.52EE205 pKa = 4.97GAPMAYY211 pKa = 9.6MNAQSIAMEE220 pKa = 4.32LRR222 pKa = 11.84AMFGWQAATPANRR235 pKa = 11.84EE236 pKa = 3.78LGNRR240 pKa = 11.84VARR243 pKa = 11.84DD244 pKa = 3.49ILRR247 pKa = 11.84DD248 pKa = 3.38GCGATRR254 pKa = 11.84EE255 pKa = 4.4QIWYY259 pKa = 7.9MSSLALHH266 pKa = 6.46MWFQPTLCDD275 pKa = 3.68LAIKK279 pKa = 10.59AGAQNFF285 pKa = 3.66

Molecular weight: 31.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q67685|MVP_GRVMC Movement protein OS=Groundnut rosette virus (strain MC1) OX=1005060 GN=ORF4 PE=1 SV=1
MM1 pKa = 7.76DD2 pKa = 4.09TTPASRR8 pKa = 11.84GQWPVGIMSSVINVFASGKK27 pKa = 8.87SCNSGGAPRR36 pKa = 11.84SSVRR40 pKa = 11.84RR41 pKa = 11.84GHH43 pKa = 6.45RR44 pKa = 11.84AGAARR49 pKa = 11.84DD50 pKa = 3.5KK51 pKa = 11.39SRR53 pKa = 11.84GFDD56 pKa = 3.26APPRR60 pKa = 11.84RR61 pKa = 11.84PKK63 pKa = 10.93GGVHH67 pKa = 7.12PATTSKK73 pKa = 11.16DD74 pKa = 3.44PNKK77 pKa = 10.34DD78 pKa = 2.68IRR80 pKa = 11.84GPPAPTPHH88 pKa = 6.57KK89 pKa = 10.0KK90 pKa = 10.13YY91 pKa = 10.01RR92 pKa = 11.84GPAIPGEE99 pKa = 4.39GRR101 pKa = 11.84SGVHH105 pKa = 3.89TTRR108 pKa = 11.84PRR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84AGRR115 pKa = 11.84SGGMDD120 pKa = 3.14PRR122 pKa = 11.84QLVAQPQQRR131 pKa = 11.84WAKK134 pKa = 9.37TEE136 pKa = 3.67IPTEE140 pKa = 3.79RR141 pKa = 11.84RR142 pKa = 11.84AEE144 pKa = 3.96IDD146 pKa = 3.33GLLPSLLNTLDD157 pKa = 3.64GQIQGDD163 pKa = 3.82AALLRR168 pKa = 11.84YY169 pKa = 9.39CVGAIKK175 pKa = 10.57RR176 pKa = 11.84EE177 pKa = 3.89LRR179 pKa = 11.84RR180 pKa = 11.84RR181 pKa = 11.84WEE183 pKa = 4.32SVQPAHH189 pKa = 6.63HH190 pKa = 6.39VAASSGKK197 pKa = 9.51PSPQLFNEE205 pKa = 4.76AAQNAEE211 pKa = 4.45DD212 pKa = 4.76LSGDD216 pKa = 3.66GKK218 pKa = 11.18GCAGQSVQQEE228 pKa = 4.46VLHH231 pKa = 6.55SGSGVPPVCADD242 pKa = 3.55CGKK245 pKa = 9.63PAANKK250 pKa = 8.04WW251 pKa = 3.34

Molecular weight: 26.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

1

5

2474

251

839

494.8

54.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.065 ± 0.566

2.425 ± 0.306

4.365 ± 0.346

5.618 ± 0.366

2.587 ± 0.285

7.801 ± 0.546

2.264 ± 0.118

3.961 ± 0.223

4.244 ± 0.483

8.084 ± 0.487

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.112 ± 0.256

3.678 ± 0.28

7.114 ± 0.725

4.163 ± 0.215

7.316 ± 0.406

5.821 ± 0.595

4.325 ± 0.169

7.801 ± 0.343

2.061 ± 0.19

3.193 ± 0.449
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski