Amino acid dipepetide frequency for Arthrobacter phage Sputnik

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.085AlaAla: 17.085 ± 3.279
0.888AlaCys: 0.888 ± 0.532
4.881AlaAsp: 4.881 ± 0.994
5.325AlaGlu: 5.325 ± 1.277
4.438AlaPhe: 4.438 ± 0.897
10.206AlaGly: 10.206 ± 1.912
1.997AlaHis: 1.997 ± 0.533
5.769AlaIle: 5.769 ± 1.508
6.213AlaLys: 6.213 ± 0.869
8.653AlaLeu: 8.653 ± 1.467
3.772AlaMet: 3.772 ± 0.816
3.106AlaAsn: 3.106 ± 0.959
6.434AlaPro: 6.434 ± 1.821
4.659AlaGln: 4.659 ± 0.72
7.988AlaArg: 7.988 ± 1.864
5.547AlaSer: 5.547 ± 1.124
9.319AlaThr: 9.319 ± 0.911
8.209AlaVal: 8.209 ± 1.824
2.441AlaTrp: 2.441 ± 0.757
2.441AlaTyr: 2.441 ± 0.817
0.0AlaXaa: 0.0 ± 0.0
Cys
0.222CysAla: 0.222 ± 0.206
0.0CysCys: 0.0 ± 0.0
0.222CysAsp: 0.222 ± 0.206
0.222CysGlu: 0.222 ± 0.269
0.222CysPhe: 0.222 ± 0.25
1.109CysGly: 1.109 ± 0.836
0.444CysHis: 0.444 ± 0.351
0.666CysIle: 0.666 ± 0.409
0.222CysLys: 0.222 ± 0.224
0.666CysLeu: 0.666 ± 0.341
0.222CysMet: 0.222 ± 0.186
0.222CysAsn: 0.222 ± 0.252
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
0.444CysArg: 0.444 ± 0.328
0.222CysSer: 0.222 ± 0.302
0.0CysThr: 0.0 ± 0.0
0.222CysVal: 0.222 ± 0.25
0.0CysTrp: 0.0 ± 0.0
0.222CysTyr: 0.222 ± 0.186
0.0CysXaa: 0.0 ± 0.0
Asp
6.213AspAla: 6.213 ± 1.359
0.0AspCys: 0.0 ± 0.0
3.328AspAsp: 3.328 ± 0.999
2.663AspGlu: 2.663 ± 0.72
1.553AspPhe: 1.553 ± 0.413
5.103AspGly: 5.103 ± 1.322
0.666AspHis: 0.666 ± 0.279
1.775AspIle: 1.775 ± 0.696
2.441AspLys: 2.441 ± 0.799
4.659AspLeu: 4.659 ± 0.975
1.553AspMet: 1.553 ± 0.826
1.775AspAsn: 1.775 ± 0.612
2.441AspPro: 2.441 ± 0.8
0.888AspGln: 0.888 ± 0.376
2.441AspArg: 2.441 ± 0.884
3.772AspSer: 3.772 ± 0.655
1.997AspThr: 1.997 ± 0.49
6.213AspVal: 6.213 ± 1.566
1.553AspTrp: 1.553 ± 0.595
1.331AspTyr: 1.331 ± 0.446
0.0AspXaa: 0.0 ± 0.0
Glu
6.656GluAla: 6.656 ± 1.117
0.888GluCys: 0.888 ± 0.54
2.663GluAsp: 2.663 ± 0.78
2.663GluGlu: 2.663 ± 0.894
0.888GluPhe: 0.888 ± 0.388
3.772GluGly: 3.772 ± 0.711
0.666GluHis: 0.666 ± 0.4
3.106GluIle: 3.106 ± 0.578
0.888GluLys: 0.888 ± 0.339
5.991GluLeu: 5.991 ± 0.966
0.888GluMet: 0.888 ± 0.409
2.219GluAsn: 2.219 ± 0.771
2.663GluPro: 2.663 ± 0.735
1.553GluGln: 1.553 ± 0.68
3.772GluArg: 3.772 ± 0.881
3.106GluSer: 3.106 ± 0.832
5.103GluThr: 5.103 ± 1.324
2.441GluVal: 2.441 ± 0.635
1.997GluTrp: 1.997 ± 0.713
0.888GluTyr: 0.888 ± 0.293
0.0GluXaa: 0.0 ± 0.0
Phe
3.772PheAla: 3.772 ± 1.047
0.0PheCys: 0.0 ± 0.0
1.997PheAsp: 1.997 ± 0.608
1.997PheGlu: 1.997 ± 0.653
0.666PhePhe: 0.666 ± 0.32
3.328PheGly: 3.328 ± 0.816
0.222PheHis: 0.222 ± 0.186
1.109PheIle: 1.109 ± 0.314
2.663PheLys: 2.663 ± 0.868
1.997PheLeu: 1.997 ± 0.825
1.331PheMet: 1.331 ± 0.591
1.553PheAsn: 1.553 ± 0.549
0.666PhePro: 0.666 ± 0.509
1.331PheGln: 1.331 ± 0.489
0.444PheArg: 0.444 ± 0.251
2.884PheSer: 2.884 ± 0.621
3.328PheThr: 3.328 ± 0.858
1.997PheVal: 1.997 ± 0.589
0.444PheTrp: 0.444 ± 0.3
1.109PheTyr: 1.109 ± 0.494
0.0PheXaa: 0.0 ± 0.0
Gly
10.428GlyAla: 10.428 ± 1.556
0.666GlyCys: 0.666 ± 0.461
4.659GlyAsp: 4.659 ± 0.715
5.103GlyGlu: 5.103 ± 1.114
3.106GlyPhe: 3.106 ± 0.783
9.319GlyGly: 9.319 ± 2.156
1.331GlyHis: 1.331 ± 0.781
4.881GlyIle: 4.881 ± 1.025
8.209GlyLys: 8.209 ± 0.785
6.656GlyLeu: 6.656 ± 1.469
1.331GlyMet: 1.331 ± 0.555
2.663GlyAsn: 2.663 ± 0.682
1.775GlyPro: 1.775 ± 0.569
4.438GlyGln: 4.438 ± 0.986
5.103GlyArg: 5.103 ± 1.164
5.769GlySer: 5.769 ± 0.684
6.878GlyThr: 6.878 ± 1.268
6.213GlyVal: 6.213 ± 1.265
1.553GlyTrp: 1.553 ± 0.457
3.328GlyTyr: 3.328 ± 0.78
0.0GlyXaa: 0.0 ± 0.0
His
2.219HisAla: 2.219 ± 0.6
0.222HisCys: 0.222 ± 0.252
0.888HisAsp: 0.888 ± 0.489
1.553HisGlu: 1.553 ± 0.732
0.666HisPhe: 0.666 ± 0.308
1.553HisGly: 1.553 ± 0.454
0.444HisHis: 0.444 ± 0.244
0.222HisIle: 0.222 ± 0.217
0.444HisLys: 0.444 ± 0.267
1.997HisLeu: 1.997 ± 0.665
0.222HisMet: 0.222 ± 0.252
0.444HisAsn: 0.444 ± 0.387
0.666HisPro: 0.666 ± 0.388
0.666HisGln: 0.666 ± 0.374
0.222HisArg: 0.222 ± 0.253
0.888HisSer: 0.888 ± 0.424
1.109HisThr: 1.109 ± 0.559
0.444HisVal: 0.444 ± 0.285
0.222HisTrp: 0.222 ± 0.224
0.222HisTyr: 0.222 ± 0.186
0.0HisXaa: 0.0 ± 0.0
Ile
7.988IleAla: 7.988 ± 1.446
0.222IleCys: 0.222 ± 0.206
3.772IleAsp: 3.772 ± 0.786
3.106IleGlu: 3.106 ± 1.209
1.553IlePhe: 1.553 ± 0.683
4.659IleGly: 4.659 ± 1.157
1.331IleHis: 1.331 ± 0.605
2.219IleIle: 2.219 ± 0.751
3.55IleLys: 3.55 ± 1.194
3.994IleLeu: 3.994 ± 1.093
1.331IleMet: 1.331 ± 0.571
2.663IleAsn: 2.663 ± 0.706
2.884IlePro: 2.884 ± 0.931
1.997IleGln: 1.997 ± 0.555
2.884IleArg: 2.884 ± 0.603
2.441IleSer: 2.441 ± 0.473
3.328IleThr: 3.328 ± 0.747
2.884IleVal: 2.884 ± 0.688
0.444IleTrp: 0.444 ± 0.263
1.331IleTyr: 1.331 ± 0.61
0.0IleXaa: 0.0 ± 0.0
Lys
4.881LysAla: 4.881 ± 1.264
0.222LysCys: 0.222 ± 0.269
2.441LysAsp: 2.441 ± 0.792
1.553LysGlu: 1.553 ± 0.535
1.775LysPhe: 1.775 ± 0.334
3.994LysGly: 3.994 ± 0.967
0.222LysHis: 0.222 ± 0.186
5.103LysIle: 5.103 ± 0.971
1.109LysLys: 1.109 ± 0.483
5.325LysLeu: 5.325 ± 1.213
1.331LysMet: 1.331 ± 0.568
1.997LysAsn: 1.997 ± 0.692
2.663LysPro: 2.663 ± 0.589
2.219LysGln: 2.219 ± 0.868
2.663LysArg: 2.663 ± 0.633
3.772LysSer: 3.772 ± 0.88
3.994LysThr: 3.994 ± 0.761
4.216LysVal: 4.216 ± 0.818
1.109LysTrp: 1.109 ± 0.417
1.109LysTyr: 1.109 ± 0.458
0.0LysXaa: 0.0 ± 0.0
Leu
5.769LeuAla: 5.769 ± 1.116
0.222LeuCys: 0.222 ± 0.252
4.438LeuAsp: 4.438 ± 1.091
4.659LeuGlu: 4.659 ± 0.815
1.553LeuPhe: 1.553 ± 0.661
7.988LeuGly: 7.988 ± 1.376
1.331LeuHis: 1.331 ± 0.481
5.103LeuIle: 5.103 ± 1.134
3.994LeuLys: 3.994 ± 1.038
5.769LeuLeu: 5.769 ± 1.117
2.441LeuMet: 2.441 ± 0.729
2.663LeuAsn: 2.663 ± 0.571
3.328LeuPro: 3.328 ± 0.831
2.663LeuGln: 2.663 ± 0.877
4.881LeuArg: 4.881 ± 1.704
7.544LeuSer: 7.544 ± 0.984
6.878LeuThr: 6.878 ± 1.36
5.991LeuVal: 5.991 ± 0.888
0.444LeuTrp: 0.444 ± 0.244
1.331LeuTyr: 1.331 ± 0.779
0.0LeuXaa: 0.0 ± 0.0
Met
3.106MetAla: 3.106 ± 0.533
0.222MetCys: 0.222 ± 0.269
1.331MetAsp: 1.331 ± 0.566
1.997MetGlu: 1.997 ± 0.594
1.109MetPhe: 1.109 ± 0.403
2.663MetGly: 2.663 ± 0.564
0.666MetHis: 0.666 ± 0.251
0.888MetIle: 0.888 ± 0.481
1.331MetLys: 1.331 ± 0.501
1.553MetLeu: 1.553 ± 0.629
0.666MetMet: 0.666 ± 0.342
1.109MetAsn: 1.109 ± 0.378
0.888MetPro: 0.888 ± 0.337
2.219MetGln: 2.219 ± 0.707
2.441MetArg: 2.441 ± 0.7
2.884MetSer: 2.884 ± 0.776
2.219MetThr: 2.219 ± 0.785
1.109MetVal: 1.109 ± 0.669
0.222MetTrp: 0.222 ± 0.175
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
4.216AsnAla: 4.216 ± 1.071
0.0AsnCys: 0.0 ± 0.0
1.331AsnAsp: 1.331 ± 0.538
3.106AsnGlu: 3.106 ± 0.888
1.775AsnPhe: 1.775 ± 0.639
3.55AsnGly: 3.55 ± 0.72
0.888AsnHis: 0.888 ± 0.338
1.331AsnIle: 1.331 ± 0.471
1.775AsnLys: 1.775 ± 0.45
1.553AsnLeu: 1.553 ± 0.579
0.666AsnMet: 0.666 ± 0.346
1.109AsnAsn: 1.109 ± 0.375
2.441AsnPro: 2.441 ± 0.73
2.219AsnGln: 2.219 ± 0.543
1.997AsnArg: 1.997 ± 0.673
2.441AsnSer: 2.441 ± 0.796
1.331AsnThr: 1.331 ± 0.654
2.219AsnVal: 2.219 ± 0.733
1.109AsnTrp: 1.109 ± 0.397
0.888AsnTyr: 0.888 ± 0.47
0.0AsnXaa: 0.0 ± 0.0
Pro
6.878ProAla: 6.878 ± 0.891
0.0ProCys: 0.0 ± 0.0
1.997ProAsp: 1.997 ± 1.113
2.441ProGlu: 2.441 ± 0.797
1.553ProPhe: 1.553 ± 0.409
4.438ProGly: 4.438 ± 0.837
1.109ProHis: 1.109 ± 0.474
2.441ProIle: 2.441 ± 0.792
3.106ProLys: 3.106 ± 0.817
2.884ProLeu: 2.884 ± 0.561
1.553ProMet: 1.553 ± 0.484
1.775ProAsn: 1.775 ± 0.63
1.331ProPro: 1.331 ± 0.388
1.331ProGln: 1.331 ± 0.491
0.888ProArg: 0.888 ± 0.44
1.331ProSer: 1.331 ± 0.495
2.884ProThr: 2.884 ± 0.906
4.438ProVal: 4.438 ± 0.838
0.888ProTrp: 0.888 ± 0.359
0.888ProTyr: 0.888 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
3.55GlnAla: 3.55 ± 1.022
0.0GlnCys: 0.0 ± 0.0
3.106GlnAsp: 3.106 ± 0.777
1.109GlnGlu: 1.109 ± 0.563
1.553GlnPhe: 1.553 ± 0.566
5.325GlnGly: 5.325 ± 0.967
0.444GlnHis: 0.444 ± 0.319
3.328GlnIle: 3.328 ± 0.758
0.888GlnLys: 0.888 ± 0.401
3.106GlnLeu: 3.106 ± 0.702
1.109GlnMet: 1.109 ± 0.443
0.222GlnAsn: 0.222 ± 0.247
1.775GlnPro: 1.775 ± 0.486
2.884GlnGln: 2.884 ± 0.988
1.775GlnArg: 1.775 ± 0.499
1.775GlnSer: 1.775 ± 0.658
3.55GlnThr: 3.55 ± 0.767
2.884GlnVal: 2.884 ± 0.461
0.444GlnTrp: 0.444 ± 0.39
0.444GlnTyr: 0.444 ± 0.267
0.0GlnXaa: 0.0 ± 0.0
Arg
7.766ArgAla: 7.766 ± 1.562
0.0ArgCys: 0.0 ± 0.0
2.441ArgAsp: 2.441 ± 0.648
2.663ArgGlu: 2.663 ± 0.821
2.663ArgPhe: 2.663 ± 0.737
3.772ArgGly: 3.772 ± 0.996
0.666ArgHis: 0.666 ± 0.355
1.553ArgIle: 1.553 ± 0.661
1.997ArgLys: 1.997 ± 1.069
3.106ArgLeu: 3.106 ± 0.942
1.997ArgMet: 1.997 ± 0.91
3.106ArgAsn: 3.106 ± 0.912
2.219ArgPro: 2.219 ± 0.678
1.553ArgGln: 1.553 ± 0.468
3.994ArgArg: 3.994 ± 0.881
2.884ArgSer: 2.884 ± 0.76
2.884ArgThr: 2.884 ± 0.535
4.659ArgVal: 4.659 ± 0.935
0.444ArgTrp: 0.444 ± 0.243
1.775ArgTyr: 1.775 ± 0.573
0.0ArgXaa: 0.0 ± 0.0
Ser
5.547SerAla: 5.547 ± 1.098
0.666SerCys: 0.666 ± 0.409
2.884SerAsp: 2.884 ± 0.792
2.663SerGlu: 2.663 ± 0.691
2.441SerPhe: 2.441 ± 0.672
8.209SerGly: 8.209 ± 1.138
0.666SerHis: 0.666 ± 0.419
3.328SerIle: 3.328 ± 0.711
1.997SerLys: 1.997 ± 0.683
6.434SerLeu: 6.434 ± 0.967
3.106SerMet: 3.106 ± 0.774
2.663SerAsn: 2.663 ± 0.692
3.106SerPro: 3.106 ± 0.731
1.109SerGln: 1.109 ± 0.348
3.328SerArg: 3.328 ± 0.708
4.659SerSer: 4.659 ± 0.724
3.772SerThr: 3.772 ± 0.809
3.55SerVal: 3.55 ± 0.82
0.888SerTrp: 0.888 ± 0.354
1.109SerTyr: 1.109 ± 0.44
0.0SerXaa: 0.0 ± 0.0
Thr
10.872ThrAla: 10.872 ± 1.786
0.444ThrCys: 0.444 ± 0.319
3.55ThrAsp: 3.55 ± 0.719
4.438ThrGlu: 4.438 ± 1.072
1.997ThrPhe: 1.997 ± 0.54
5.325ThrGly: 5.325 ± 1.086
0.666ThrHis: 0.666 ± 0.306
4.659ThrIle: 4.659 ± 0.934
3.328ThrLys: 3.328 ± 0.893
4.438ThrLeu: 4.438 ± 0.764
1.997ThrMet: 1.997 ± 0.532
3.106ThrAsn: 3.106 ± 0.681
2.884ThrPro: 2.884 ± 0.778
2.441ThrGln: 2.441 ± 0.665
3.106ThrArg: 3.106 ± 0.663
2.884ThrSer: 2.884 ± 0.753
4.659ThrThr: 4.659 ± 0.856
7.766ThrVal: 7.766 ± 1.228
1.109ThrTrp: 1.109 ± 0.545
0.888ThrTyr: 0.888 ± 0.411
0.0ThrXaa: 0.0 ± 0.0
Val
6.656ValAla: 6.656 ± 1.148
0.444ValCys: 0.444 ± 0.286
3.328ValAsp: 3.328 ± 1.174
3.106ValGlu: 3.106 ± 0.701
2.219ValPhe: 2.219 ± 0.893
5.547ValGly: 5.547 ± 1.415
1.109ValHis: 1.109 ± 0.469
4.881ValIle: 4.881 ± 2.377
5.103ValLys: 5.103 ± 1.386
7.322ValLeu: 7.322 ± 1.291
1.997ValMet: 1.997 ± 0.478
2.663ValAsn: 2.663 ± 0.617
3.772ValPro: 3.772 ± 0.782
3.55ValGln: 3.55 ± 0.661
2.884ValArg: 2.884 ± 0.982
4.659ValSer: 4.659 ± 1.122
5.325ValThr: 5.325 ± 1.257
5.325ValVal: 5.325 ± 1.207
1.331ValTrp: 1.331 ± 0.551
1.331ValTyr: 1.331 ± 0.498
0.0ValXaa: 0.0 ± 0.0
Trp
3.106TrpAla: 3.106 ± 0.597
0.222TrpCys: 0.222 ± 0.224
1.775TrpAsp: 1.775 ± 0.436
1.109TrpGlu: 1.109 ± 0.475
0.222TrpPhe: 0.222 ± 0.252
1.331TrpGly: 1.331 ± 0.358
0.222TrpHis: 0.222 ± 0.205
1.331TrpIle: 1.331 ± 0.532
1.109TrpLys: 1.109 ± 0.376
1.775TrpLeu: 1.775 ± 0.92
0.222TrpMet: 0.222 ± 0.23
0.444TrpAsn: 0.444 ± 0.277
0.888TrpPro: 0.888 ± 0.506
0.888TrpGln: 0.888 ± 0.373
0.222TrpArg: 0.222 ± 0.186
0.666TrpSer: 0.666 ± 0.291
0.222TrpThr: 0.222 ± 0.217
0.888TrpVal: 0.888 ± 0.469
0.222TrpTrp: 0.222 ± 0.175
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.663TyrAla: 2.663 ± 0.586
0.222TyrCys: 0.222 ± 0.186
1.331TyrAsp: 1.331 ± 0.59
1.331TyrGlu: 1.331 ± 0.599
0.888TyrPhe: 0.888 ± 0.406
2.219TyrGly: 2.219 ± 0.685
0.222TyrHis: 0.222 ± 0.186
0.888TyrIle: 0.888 ± 0.45
1.331TyrLys: 1.331 ± 0.548
1.109TyrLeu: 1.109 ± 0.474
0.666TyrMet: 0.666 ± 0.298
0.444TyrAsn: 0.444 ± 0.258
1.331TyrPro: 1.331 ± 0.556
0.666TyrGln: 0.666 ± 0.472
0.666TyrArg: 0.666 ± 0.505
1.997TyrSer: 1.997 ± 0.76
1.775TyrThr: 1.775 ± 0.675
0.888TyrVal: 0.888 ± 0.567
0.0TyrTrp: 0.0 ± 0.0
0.222TyrTyr: 0.222 ± 0.186
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 23 proteins (4508 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski