Tumebacillus flagellatus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Tumebacillus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A074M4E1|A0A074M4E1_9BACL 4-hydroxybutyrate CoA-transferase OS=Tumebacillus flagellatus OX=1157490 GN=EL26_23870 PE=3 SV=1
MM1 pKa = 7.2LQDD4 pKa = 3.34AQTRR8 pKa = 11.84QAVMAGGRR16 pKa = 11.84VFIYY20 pKa = 10.25TPAFADD26 pKa = 4.74GSASLEE32 pKa = 4.07IRR34 pKa = 11.84NGDD37 pKa = 3.81CNEE40 pKa = 3.47EE41 pKa = 3.94LMYY44 pKa = 10.86DD45 pKa = 4.25GYY47 pKa = 11.51QPFEE51 pKa = 3.98AFEE54 pKa = 4.34AVLNAYY60 pKa = 8.92EE61 pKa = 3.97NAARR65 pKa = 11.84FVDD68 pKa = 4.05PDD70 pKa = 3.88TFQPIYY76 pKa = 10.98SGDD79 pKa = 3.69TAVDD83 pKa = 4.3GPFF86 pKa = 3.24

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A074LLW3|A0A074LLW3_9BACL DUF2220 domain-containing protein OS=Tumebacillus flagellatus OX=1157490 GN=EL26_23810 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.09IGSPLWKK9 pKa = 9.98FKK11 pKa = 10.25TRR13 pKa = 11.84LRR15 pKa = 11.84RR16 pKa = 11.84NPRR19 pKa = 11.84LLLAPLLLLLVLLLGSFAVKK39 pKa = 9.97AAHH42 pKa = 6.45GLAAAAAMLWITVGVAVGFLLGRR65 pKa = 11.84ITTPKK70 pKa = 9.81PFPRR74 pKa = 11.84KK75 pKa = 8.32KK76 pKa = 9.02TRR78 pKa = 3.04

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4452

0

4452

1391821

37

4569

312.6

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.896 ± 0.041

0.764 ± 0.01

5.266 ± 0.028

6.816 ± 0.045

3.987 ± 0.027

7.507 ± 0.042

2.243 ± 0.017

5.425 ± 0.034

4.79 ± 0.035

10.594 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.018

3.521 ± 0.026

4.249 ± 0.025

4.232 ± 0.032

5.578 ± 0.036

5.624 ± 0.027

5.721 ± 0.03

7.747 ± 0.034

1.256 ± 0.015

3.137 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski