Petromyces alliaceus (Aspergillus alliaceus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13098 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N6FJM5|A0A5N6FJM5_PETAA Ankyrin repeat-containing domain protein OS=Petromyces alliaceus OX=209559 GN=BDW43DRAFT_314963 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 10.31VVSALPLLAIAATASSVRR20 pKa = 11.84NIFNLISSGGSDD32 pKa = 3.61DD33 pKa = 3.81SHH35 pKa = 7.86NGLYY39 pKa = 10.71LSTQHH44 pKa = 7.04IDD46 pKa = 3.55PLNSDD51 pKa = 3.47AVFRR55 pKa = 11.84DD56 pKa = 3.23SDD58 pKa = 3.94DD59 pKa = 4.0AADD62 pKa = 4.84FYY64 pKa = 11.48LDD66 pKa = 3.65NGTVRR71 pKa = 11.84YY72 pKa = 8.15VAPNGAPFALALDD85 pKa = 4.4SGDD88 pKa = 3.62EE89 pKa = 4.23VQGSVEE95 pKa = 4.01ISVSPLFGSTGFNITSDD112 pKa = 3.69NTLEE116 pKa = 4.17TANQSWGGWLVCDD129 pKa = 5.07RR130 pKa = 11.84SDD132 pKa = 3.96GLLGLYY138 pKa = 9.84YY139 pKa = 9.88EE140 pKa = 4.62NNGAGSNLLEE150 pKa = 4.38EE151 pKa = 5.18CDD153 pKa = 5.05AIALDD158 pKa = 4.15VVYY161 pKa = 10.74KK162 pKa = 10.33PISS165 pKa = 3.24

Molecular weight: 17.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N6FB38|A0A5N6FB38_PETAA Uncharacterized protein OS=Petromyces alliaceus OX=209559 GN=BDW43DRAFT_305065 PE=4 SV=1
MM1 pKa = 6.22VQSMIRR7 pKa = 11.84TPANMRR13 pKa = 11.84RR14 pKa = 11.84SRR16 pKa = 11.84LFVFISLHH24 pKa = 4.75TSFVLVRR31 pKa = 11.84LHH33 pKa = 6.23SQIQAKK39 pKa = 9.85IAFFFWWSTTNSRR52 pKa = 11.84LASS55 pKa = 3.57

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13098

0

13098

5862436

49

6900

447.6

49.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.169 ± 0.02

1.371 ± 0.009

5.48 ± 0.015

5.969 ± 0.021

3.867 ± 0.014

6.782 ± 0.017

2.459 ± 0.01

5.211 ± 0.015

4.583 ± 0.019

9.313 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.008

3.695 ± 0.011

5.876 ± 0.022

3.979 ± 0.013

6.083 ± 0.018

8.359 ± 0.023

5.908 ± 0.013

6.259 ± 0.015

1.512 ± 0.008

2.948 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski