Tortoise microvirus 46

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W635|A0A4P8W635_9VIRU Uncharacterized protein OS=Tortoise microvirus 46 OX=2583150 PE=4 SV=1
MM1 pKa = 7.34SSKK4 pKa = 10.69FNFLDD9 pKa = 3.48YY10 pKa = 11.29GRR12 pKa = 11.84LSEE15 pKa = 4.43WDD17 pKa = 3.62TVPDD21 pKa = 4.26FNSNLGFGWYY31 pKa = 9.46IPGAPEE37 pKa = 3.55EE38 pKa = 4.49RR39 pKa = 11.84NVHH42 pKa = 4.71VLARR46 pKa = 11.84ASVPCTVIVSRR57 pKa = 11.84PRR59 pKa = 11.84VIDD62 pKa = 4.31GYY64 pKa = 9.35PVEE67 pKa = 5.02DD68 pKa = 3.25AVFNAGANKK77 pKa = 8.43WQWIDD82 pKa = 3.63EE83 pKa = 4.3EE84 pKa = 4.51SGEE87 pKa = 4.32ILVEE91 pKa = 4.57DD92 pKa = 4.37YY93 pKa = 11.55SDD95 pKa = 4.78GSILLQFDD103 pKa = 4.01GVAEE107 pKa = 3.77IRR109 pKa = 11.84YY110 pKa = 7.31TVDD113 pKa = 2.72TQSIFNILPVDD124 pKa = 3.97PSSPLLPSGVGVKK137 pKa = 9.38FVGDD141 pKa = 3.9APVAAPSEE149 pKa = 4.46EE150 pKa = 3.84EE151 pKa = 4.55SYY153 pKa = 11.04TSVVPGQGQSTDD165 pKa = 3.46LEE167 pKa = 4.46RR168 pKa = 11.84MMRR171 pKa = 11.84LLALNEE177 pKa = 3.85ARR179 pKa = 11.84RR180 pKa = 11.84EE181 pKa = 4.15AQYY184 pKa = 11.28NEE186 pKa = 3.44AMAAFRR192 pKa = 11.84DD193 pKa = 4.48PVTGLPPVEE202 pKa = 4.71VVDD205 pKa = 4.76DD206 pKa = 4.21DD207 pKa = 4.74APVVEE212 pKa = 4.78ATEE215 pKa = 4.13PTPP218 pKa = 4.22

Molecular weight: 23.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W691|A0A4P8W691_9VIRU Peptidase_M15_4 domain-containing protein OS=Tortoise microvirus 46 OX=2583150 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84EE5 pKa = 4.03FLSEE9 pKa = 3.88QAAKK13 pKa = 9.18PAKK16 pKa = 9.26PSPLAGTFQRR26 pKa = 11.84PALLPVVATTGKK38 pKa = 9.85QSRR41 pKa = 11.84KK42 pKa = 8.6ATTTHH47 pKa = 5.4EE48 pKa = 4.31TGSNSTLPPNKK59 pKa = 9.74DD60 pKa = 2.84EE61 pKa = 4.81KK62 pKa = 11.47SRR64 pKa = 11.84VTCKK68 pKa = 10.01EE69 pKa = 3.61RR70 pKa = 11.84PDD72 pKa = 3.49GKK74 pKa = 10.76KK75 pKa = 9.94RR76 pKa = 11.84GSGKK80 pKa = 10.42GRR82 pKa = 11.84DD83 pKa = 3.65FVPWCGG89 pKa = 3.25

Molecular weight: 9.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1710

86

552

244.3

27.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.058 ± 0.869

0.877 ± 0.324

6.199 ± 0.603

5.673 ± 0.578

4.152 ± 0.236

8.129 ± 0.848

1.871 ± 0.559

3.626 ± 0.614

5.205 ± 0.948

8.187 ± 0.687

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.164 ± 0.358

3.86 ± 0.979

5.556 ± 0.802

4.211 ± 0.434

7.368 ± 0.511

5.088 ± 0.7

5.673 ± 0.749

7.193 ± 0.736

1.696 ± 0.294

3.216 ± 0.436
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski