Citrus leaf blotch virus (isolate Nagami kumquat/France/SRA-153/1984) (CLBV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Citrivirus; Citrus leaf blotch virus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q91QZ2|MOVP_CLBVS Putative movement protein OS=Citrus leaf blotch virus (isolate Nagami kumquat/France/SRA-153/1984) OX=686981 GN=ORF2 PE=4 SV=1
MM1 pKa = 8.02KK2 pKa = 9.34ITNDD6 pKa = 2.86NAATINYY13 pKa = 7.34WLAIVEE19 pKa = 4.31PFLTSDD25 pKa = 3.5EE26 pKa = 4.84DD27 pKa = 4.18RR28 pKa = 11.84NSDD31 pKa = 3.91DD32 pKa = 4.06IIRR35 pKa = 11.84KK36 pKa = 8.45FRR38 pKa = 11.84AVVAEE43 pKa = 4.29HH44 pKa = 7.19GDD46 pKa = 3.71TEE48 pKa = 4.43EE49 pKa = 3.9VDD51 pKa = 3.62PEE53 pKa = 4.24VFFAIFSILATKK65 pKa = 9.77YY66 pKa = 10.14GRR68 pKa = 11.84VYY70 pKa = 10.32SKK72 pKa = 10.66KK73 pKa = 10.03VEE75 pKa = 4.09EE76 pKa = 4.44LNEE79 pKa = 4.11SLKK82 pKa = 11.07AAILAGAEE90 pKa = 4.29AEE92 pKa = 4.18DD93 pKa = 4.97LRR95 pKa = 11.84NKK97 pKa = 10.56LKK99 pKa = 10.77DD100 pKa = 2.93ISQRR104 pKa = 11.84YY105 pKa = 8.57ASQLEE110 pKa = 4.0ITADD114 pKa = 3.63RR115 pKa = 11.84EE116 pKa = 4.16QQLEE120 pKa = 4.1SLKK123 pKa = 10.93KK124 pKa = 10.0KK125 pKa = 10.05GHH127 pKa = 5.38EE128 pKa = 4.05QPLTGSGSSEE138 pKa = 3.79PVHH141 pKa = 7.01AEE143 pKa = 3.89SAHH146 pKa = 5.87APQLHH151 pKa = 5.65VVNDD155 pKa = 3.9LQQFYY160 pKa = 10.66IPFNEE165 pKa = 4.24YY166 pKa = 10.05PSLTQSIGTSDD177 pKa = 3.28IANDD181 pKa = 3.35EE182 pKa = 4.11HH183 pKa = 7.2LKK185 pKa = 10.51RR186 pKa = 11.84VQLTLKK192 pKa = 9.17ITDD195 pKa = 3.52TKK197 pKa = 10.79VFSRR201 pKa = 11.84TGFEE205 pKa = 4.12FAISCGSRR213 pKa = 11.84STSDD217 pKa = 3.05KK218 pKa = 11.14DD219 pKa = 3.56PYY221 pKa = 11.19DD222 pKa = 4.47GIIKK226 pKa = 10.22ISGKK230 pKa = 7.87SHH232 pKa = 4.67MRR234 pKa = 11.84KK235 pKa = 10.06DD236 pKa = 2.68IAYY239 pKa = 9.37AIRR242 pKa = 11.84TSGITVRR249 pKa = 11.84QFCAAFANLYY259 pKa = 9.97WNFNLARR266 pKa = 11.84NTPPEE271 pKa = 3.7NWRR274 pKa = 11.84KK275 pKa = 10.23KK276 pKa = 10.68GFTEE280 pKa = 4.3GTKK283 pKa = 10.17FAAFDD288 pKa = 3.29FFYY291 pKa = 10.95AVGSNAAIPTEE302 pKa = 4.02ADD304 pKa = 3.11GSVRR308 pKa = 11.84LIRR311 pKa = 11.84PPTNEE316 pKa = 3.61EE317 pKa = 3.85NEE319 pKa = 4.26ANSAMRR325 pKa = 11.84YY326 pKa = 9.39ADD328 pKa = 3.54IYY330 pKa = 9.42EE331 pKa = 4.36QNSKK335 pKa = 7.91TAGHH339 pKa = 5.6VTSSPLYY346 pKa = 9.81NRR348 pKa = 11.84GSSYY352 pKa = 10.54EE353 pKa = 4.22SKK355 pKa = 11.12NKK357 pKa = 10.48AKK359 pKa = 10.46LLEE362 pKa = 4.16MM363 pKa = 5.41

Molecular weight: 40.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q91QZ3|RDRP_CLBVS RNA replication polyprotein OS=Citrus leaf blotch virus (isolate Nagami kumquat/France/SRA-153/1984) OX=686981 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.73ASLINVSSLVNRR13 pKa = 11.84VKK15 pKa = 10.75LDD17 pKa = 3.31QSIIGSDD24 pKa = 4.49EE25 pKa = 3.75INKK28 pKa = 10.14LYY30 pKa = 10.95GSDD33 pKa = 3.46APLVFKK39 pKa = 11.12DD40 pKa = 3.84EE41 pKa = 4.32VKK43 pKa = 10.33MVIPGNAEE51 pKa = 3.48GEE53 pKa = 4.38AIKK56 pKa = 10.63LQANILTADD65 pKa = 4.11RR66 pKa = 11.84LQSIRR71 pKa = 11.84NAKK74 pKa = 10.0VNGKK78 pKa = 8.79EE79 pKa = 3.54AAYY82 pKa = 10.06LHH84 pKa = 6.59LGFVPIAIRR93 pKa = 11.84SLLPSGNEE101 pKa = 4.1QIWGRR106 pKa = 11.84CALVDD111 pKa = 3.58TSRR114 pKa = 11.84TRR116 pKa = 11.84AEE118 pKa = 3.81TAVIDD123 pKa = 3.71EE124 pKa = 4.96FEE126 pKa = 4.31FKK128 pKa = 9.35FTKK131 pKa = 9.95KK132 pKa = 10.36QPFAAKK138 pKa = 10.46LLTINAAVDD147 pKa = 3.55INCKK151 pKa = 10.2VSVGSIQVLLEE162 pKa = 3.67LHH164 pKa = 6.49GVDD167 pKa = 4.03LRR169 pKa = 11.84EE170 pKa = 4.1EE171 pKa = 4.13RR172 pKa = 11.84SVAAIITGLTCTPTNKK188 pKa = 9.03MVLLHH193 pKa = 6.93KK194 pKa = 10.24IEE196 pKa = 5.15CDD198 pKa = 3.3TPKK201 pKa = 10.31WSLCNIIEE209 pKa = 4.14QVEE212 pKa = 4.12DD213 pKa = 3.57EE214 pKa = 4.71EE215 pKa = 4.35EE216 pKa = 4.13SKK218 pKa = 11.0KK219 pKa = 10.96AFEE222 pKa = 4.39NMFNASSSNLIDD234 pKa = 4.7LGQEE238 pKa = 3.4QWLDD242 pKa = 3.33EE243 pKa = 4.5GKK245 pKa = 8.52RR246 pKa = 11.84TPLIGSLAIKK256 pKa = 10.58GFGRR260 pKa = 11.84KK261 pKa = 8.29VMPVRR266 pKa = 11.84RR267 pKa = 11.84RR268 pKa = 11.84NLTTRR273 pKa = 11.84NLMKK277 pKa = 10.48DD278 pKa = 3.5YY279 pKa = 10.51VSHH282 pKa = 6.28VKK284 pKa = 10.56SEE286 pKa = 4.06TASLKK291 pKa = 10.18RR292 pKa = 11.84SQSGRR297 pKa = 11.84DD298 pKa = 3.01WGNDD302 pKa = 2.93RR303 pKa = 11.84LRR305 pKa = 11.84KK306 pKa = 9.57YY307 pKa = 10.79LEE309 pKa = 4.07EE310 pKa = 3.98QALEE314 pKa = 4.11QARR317 pKa = 11.84SSTDD321 pKa = 3.06HH322 pKa = 6.46QLVKK326 pKa = 10.69APKK329 pKa = 8.91FKK331 pKa = 10.29TIEE334 pKa = 3.98VTGLEE339 pKa = 4.43TVHH342 pKa = 6.93DD343 pKa = 4.88LKK345 pKa = 11.5DD346 pKa = 4.28KK347 pKa = 10.53IDD349 pKa = 3.65KK350 pKa = 10.89AEE352 pKa = 4.15SSTASDD358 pKa = 3.44TGTKK362 pKa = 9.97

Molecular weight: 40.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2687

362

1962

895.7

102.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.215 ± 1.539

2.01 ± 0.613

5.769 ± 0.014

8.336 ± 0.26

6.699 ± 1.564

4.987 ± 0.158

2.642 ± 0.392

6.401 ± 0.197

7.146 ± 0.395

9.155 ± 0.772

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.861 ± 0.294

5.359 ± 0.137

3.052 ± 0.246

3.089 ± 0.198

5.434 ± 0.06

7.294 ± 0.652

4.801 ± 0.786

5.62 ± 0.413

1.042 ± 0.067

3.089 ± 0.529
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski