Dothistroma septosporum (strain NZE10 / CBS 128990) (Red band needle blight fungus) (Mycosphaerella pini)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Dothistroma; Dothistroma septosporum

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M2YKY2|M2YKY2_DOTSN Uncharacterized protein OS=Dothistroma septosporum (strain NZE10 / CBS 128990) OX=675120 GN=DOTSEDRAFT_48006 PE=4 SV=1
MM1 pKa = 7.38LTTLALALLATSTTASPTKK20 pKa = 10.11RR21 pKa = 11.84QTQVRR26 pKa = 11.84TKK28 pKa = 9.69NYY30 pKa = 10.37SGAKK34 pKa = 9.57LLSSPDD40 pKa = 3.36GTIFTYY46 pKa = 9.12ATTIINVPDD55 pKa = 3.76TQFVSAEE62 pKa = 4.02GSPGQLQASAAWVGYY77 pKa = 10.76GGMEE81 pKa = 4.78DD82 pKa = 3.87NGIAQTGCVFDD93 pKa = 5.85NDD95 pKa = 4.41GQDD98 pKa = 4.1DD99 pKa = 4.42RR100 pKa = 11.84YY101 pKa = 10.55ARR103 pKa = 11.84CWTEE107 pKa = 4.66FYY109 pKa = 10.07PDD111 pKa = 3.9PPVFYY116 pKa = 10.33DD117 pKa = 4.87DD118 pKa = 5.68LPSPDD123 pKa = 3.34IGDD126 pKa = 4.47WIQLEE131 pKa = 4.79LYY133 pKa = 8.06LTSPTTATSIVTNTNTGDD151 pKa = 3.24QASRR155 pKa = 11.84DD156 pKa = 3.65FTAPDD161 pKa = 3.26GHH163 pKa = 6.28SVSGNNAEE171 pKa = 4.47WIVEE175 pKa = 4.15NPGARR180 pKa = 11.84NNLVNFQKK188 pKa = 10.79EE189 pKa = 4.21FFEE192 pKa = 4.93GCQAVTQAGNSYY204 pKa = 10.98GPGDD208 pKa = 3.71GNVEE212 pKa = 3.93VFYY215 pKa = 10.28EE216 pKa = 4.41AKK218 pKa = 10.61DD219 pKa = 3.62LGDD222 pKa = 4.25GNGATQWTNSFLPSDD237 pKa = 4.24SEE239 pKa = 5.3VEE241 pKa = 4.2VDD243 pKa = 3.12WTGPTT248 pKa = 3.35

Molecular weight: 26.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M2YMF6|M2YMF6_DOTSN 5-aminolevulinate synthase OS=Dothistroma septosporum (strain NZE10 / CBS 128990) OX=675120 GN=DOTSEDRAFT_72469 PE=3 SV=1
MM1 pKa = 7.87PSHH4 pKa = 5.83KK5 pKa = 9.44TFRR8 pKa = 11.84TKK10 pKa = 10.67VKK12 pKa = 9.81LAKK15 pKa = 9.79AQKK18 pKa = 8.73QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12399

15

12414

5094136

49

5888

410.4

45.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.079 ± 0.019

1.297 ± 0.009

5.844 ± 0.017

6.093 ± 0.023

3.492 ± 0.013

6.953 ± 0.023

2.549 ± 0.011

4.654 ± 0.014

4.901 ± 0.021

8.561 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.009

3.61 ± 0.012

5.801 ± 0.02

4.294 ± 0.017

6.295 ± 0.021

7.982 ± 0.027

6.046 ± 0.017

6.075 ± 0.016

1.456 ± 0.009

2.755 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski