Amino acid dipepetide frequency for Streptomyces phage Lannister

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.779AlaAla: 10.779 ± 1.014
0.526AlaCys: 0.526 ± 0.241
6.967AlaAsp: 6.967 ± 0.665
8.018AlaGlu: 8.018 ± 0.883
3.286AlaPhe: 3.286 ± 0.407
7.295AlaGly: 7.295 ± 0.702
2.037AlaHis: 2.037 ± 0.496
4.666AlaIle: 4.666 ± 0.471
4.995AlaLys: 4.995 ± 0.543
9.924AlaLeu: 9.924 ± 1.022
3.221AlaMet: 3.221 ± 0.414
3.089AlaAsn: 3.089 ± 0.429
5.061AlaPro: 5.061 ± 0.816
3.549AlaGln: 3.549 ± 0.497
6.244AlaArg: 6.244 ± 0.684
5.652AlaSer: 5.652 ± 0.666
5.652AlaThr: 5.652 ± 0.761
9.136AlaVal: 9.136 ± 0.759
2.169AlaTrp: 2.169 ± 0.337
3.286AlaTyr: 3.286 ± 0.454
0.0AlaXaa: 0.0 ± 0.0
Cys
0.657CysAla: 0.657 ± 0.18
0.066CysCys: 0.066 ± 0.069
0.329CysAsp: 0.329 ± 0.149
0.657CysGlu: 0.657 ± 0.237
0.131CysPhe: 0.131 ± 0.102
0.46CysGly: 0.46 ± 0.177
0.197CysHis: 0.197 ± 0.108
0.263CysIle: 0.263 ± 0.125
0.394CysLys: 0.394 ± 0.18
0.394CysLeu: 0.394 ± 0.167
0.066CysMet: 0.066 ± 0.069
0.197CysAsn: 0.197 ± 0.101
0.723CysPro: 0.723 ± 0.283
0.066CysGln: 0.066 ± 0.059
0.526CysArg: 0.526 ± 0.206
0.592CysSer: 0.592 ± 0.273
0.263CysThr: 0.263 ± 0.144
0.592CysVal: 0.592 ± 0.15
0.066CysTrp: 0.066 ± 0.067
0.263CysTyr: 0.263 ± 0.15
0.0CysXaa: 0.0 ± 0.0
Asp
6.638AspAla: 6.638 ± 0.748
0.394AspCys: 0.394 ± 0.163
3.418AspAsp: 3.418 ± 0.579
4.404AspGlu: 4.404 ± 0.496
2.3AspPhe: 2.3 ± 0.404
6.638AspGly: 6.638 ± 0.75
1.052AspHis: 1.052 ± 0.301
2.629AspIle: 2.629 ± 0.387
2.103AspLys: 2.103 ± 0.355
6.178AspLeu: 6.178 ± 0.696
1.577AspMet: 1.577 ± 0.287
1.84AspAsn: 1.84 ± 0.422
4.206AspPro: 4.206 ± 0.542
2.103AspGln: 2.103 ± 0.315
3.483AspArg: 3.483 ± 0.554
3.418AspSer: 3.418 ± 0.353
3.812AspThr: 3.812 ± 0.486
3.681AspVal: 3.681 ± 0.566
1.906AspTrp: 1.906 ± 0.287
1.643AspTyr: 1.643 ± 0.385
0.0AspXaa: 0.0 ± 0.0
Glu
7.69GluAla: 7.69 ± 0.907
0.723GluCys: 0.723 ± 0.229
4.666GluAsp: 4.666 ± 0.592
6.178GluGlu: 6.178 ± 1.071
2.037GluPhe: 2.037 ± 0.301
6.178GluGly: 6.178 ± 0.659
1.643GluHis: 1.643 ± 0.379
4.141GluIle: 4.141 ± 0.511
2.366GluLys: 2.366 ± 0.396
6.178GluLeu: 6.178 ± 0.648
1.183GluMet: 1.183 ± 0.264
2.103GluAsn: 2.103 ± 0.333
2.432GluPro: 2.432 ± 0.357
2.3GluGln: 2.3 ± 0.367
3.878GluArg: 3.878 ± 0.469
4.469GluSer: 4.469 ± 0.691
4.666GluThr: 4.666 ± 0.656
6.112GluVal: 6.112 ± 0.619
1.446GluTrp: 1.446 ± 0.304
2.103GluTyr: 2.103 ± 0.389
0.0GluXaa: 0.0 ± 0.0
Phe
3.418PheAla: 3.418 ± 0.377
0.263PheCys: 0.263 ± 0.12
2.695PheAsp: 2.695 ± 0.44
2.695PheGlu: 2.695 ± 0.395
1.249PhePhe: 1.249 ± 0.27
2.958PheGly: 2.958 ± 0.434
0.592PheHis: 0.592 ± 0.187
1.577PheIle: 1.577 ± 0.376
1.314PheLys: 1.314 ± 0.297
2.235PheLeu: 2.235 ± 0.355
0.789PheMet: 0.789 ± 0.234
0.92PheAsn: 0.92 ± 0.232
1.249PhePro: 1.249 ± 0.281
1.117PheGln: 1.117 ± 0.247
2.366PheArg: 2.366 ± 0.388
1.84PheSer: 1.84 ± 0.335
2.498PheThr: 2.498 ± 0.39
1.775PheVal: 1.775 ± 0.279
0.394PheTrp: 0.394 ± 0.143
0.789PheTyr: 0.789 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
8.281GlyAla: 8.281 ± 1.04
0.46GlyCys: 0.46 ± 0.189
5.849GlyAsp: 5.849 ± 0.7
5.061GlyGlu: 5.061 ± 0.551
3.089GlyPhe: 3.089 ± 0.556
7.427GlyGly: 7.427 ± 0.883
2.169GlyHis: 2.169 ± 0.355
3.352GlyIle: 3.352 ± 0.65
4.075GlyLys: 4.075 ± 0.638
7.033GlyLeu: 7.033 ± 0.943
1.775GlyMet: 1.775 ± 0.373
2.432GlyAsn: 2.432 ± 0.336
3.615GlyPro: 3.615 ± 0.739
2.958GlyGln: 2.958 ± 0.36
3.943GlyArg: 3.943 ± 0.465
5.587GlySer: 5.587 ± 0.77
5.718GlyThr: 5.718 ± 0.855
6.704GlyVal: 6.704 ± 0.621
2.103GlyTrp: 2.103 ± 0.341
3.023GlyTyr: 3.023 ± 0.551
0.0GlyXaa: 0.0 ± 0.0
His
1.709HisAla: 1.709 ± 0.374
0.263HisCys: 0.263 ± 0.114
1.249HisAsp: 1.249 ± 0.282
1.117HisGlu: 1.117 ± 0.265
0.92HisPhe: 0.92 ± 0.196
1.709HisGly: 1.709 ± 0.416
0.526HisHis: 0.526 ± 0.188
0.592HisIle: 0.592 ± 0.211
0.592HisLys: 0.592 ± 0.193
1.775HisLeu: 1.775 ± 0.311
0.066HisMet: 0.066 ± 0.05
0.657HisAsn: 0.657 ± 0.204
1.249HisPro: 1.249 ± 0.256
0.789HisGln: 0.789 ± 0.2
1.183HisArg: 1.183 ± 0.259
1.249HisSer: 1.249 ± 0.314
1.577HisThr: 1.577 ± 0.342
1.512HisVal: 1.512 ± 0.339
0.657HisTrp: 0.657 ± 0.249
0.789HisTyr: 0.789 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
4.272IleAla: 4.272 ± 0.528
0.066IleCys: 0.066 ± 0.067
3.681IleAsp: 3.681 ± 0.401
4.206IleGlu: 4.206 ± 0.504
1.314IlePhe: 1.314 ± 0.26
3.549IleGly: 3.549 ± 0.509
0.723IleHis: 0.723 ± 0.206
2.3IleIle: 2.3 ± 0.746
2.037IleLys: 2.037 ± 0.435
3.418IleLeu: 3.418 ± 0.718
0.592IleMet: 0.592 ± 0.235
1.052IleAsn: 1.052 ± 0.289
1.972IlePro: 1.972 ± 0.318
1.577IleGln: 1.577 ± 0.399
3.483IleArg: 3.483 ± 0.377
1.906IleSer: 1.906 ± 0.435
2.432IleThr: 2.432 ± 0.447
3.483IleVal: 3.483 ± 0.432
0.592IleTrp: 0.592 ± 0.172
1.314IleTyr: 1.314 ± 0.243
0.0IleXaa: 0.0 ± 0.0
Lys
4.798LysAla: 4.798 ± 0.617
0.197LysCys: 0.197 ± 0.136
2.826LysAsp: 2.826 ± 0.415
2.169LysGlu: 2.169 ± 0.321
0.986LysPhe: 0.986 ± 0.256
5.061LysGly: 5.061 ± 0.563
0.854LysHis: 0.854 ± 0.225
1.972LysIle: 1.972 ± 0.428
2.366LysLys: 2.366 ± 0.518
3.812LysLeu: 3.812 ± 0.474
0.592LysMet: 0.592 ± 0.209
1.512LysAsn: 1.512 ± 0.353
3.023LysPro: 3.023 ± 0.522
1.84LysGln: 1.84 ± 0.278
3.352LysArg: 3.352 ± 0.509
1.577LysSer: 1.577 ± 0.352
3.089LysThr: 3.089 ± 0.381
2.235LysVal: 2.235 ± 0.366
0.657LysTrp: 0.657 ± 0.214
1.052LysTyr: 1.052 ± 0.272
0.0LysXaa: 0.0 ± 0.0
Leu
10.384LeuAla: 10.384 ± 0.936
0.592LeuCys: 0.592 ± 0.191
6.507LeuAsp: 6.507 ± 0.747
5.784LeuGlu: 5.784 ± 0.804
1.906LeuPhe: 1.906 ± 0.371
6.572LeuGly: 6.572 ± 0.768
1.577LeuHis: 1.577 ± 0.289
3.681LeuIle: 3.681 ± 0.482
3.352LeuLys: 3.352 ± 0.43
6.77LeuLeu: 6.77 ± 0.659
1.775LeuMet: 1.775 ± 0.323
3.483LeuAsn: 3.483 ± 0.49
4.929LeuPro: 4.929 ± 0.638
2.037LeuGln: 2.037 ± 0.38
6.178LeuArg: 6.178 ± 0.684
5.258LeuSer: 5.258 ± 0.704
5.061LeuThr: 5.061 ± 0.654
5.915LeuVal: 5.915 ± 0.778
1.183LeuTrp: 1.183 ± 0.237
2.037LeuTyr: 2.037 ± 0.289
0.0LeuXaa: 0.0 ± 0.0
Met
3.286MetAla: 3.286 ± 0.405
0.066MetCys: 0.066 ± 0.066
0.657MetAsp: 0.657 ± 0.223
0.854MetGlu: 0.854 ± 0.224
0.46MetPhe: 0.46 ± 0.206
1.512MetGly: 1.512 ± 0.476
0.263MetHis: 0.263 ± 0.129
1.117MetIle: 1.117 ± 0.267
1.052MetLys: 1.052 ± 0.266
1.775MetLeu: 1.775 ± 0.358
0.263MetMet: 0.263 ± 0.125
0.657MetAsn: 0.657 ± 0.209
1.183MetPro: 1.183 ± 0.239
0.526MetGln: 0.526 ± 0.165
1.577MetArg: 1.577 ± 0.319
1.709MetSer: 1.709 ± 0.338
1.972MetThr: 1.972 ± 0.377
1.249MetVal: 1.249 ± 0.219
0.131MetTrp: 0.131 ± 0.094
0.329MetTyr: 0.329 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
3.418AsnAla: 3.418 ± 0.48
0.723AsnCys: 0.723 ± 0.284
1.512AsnAsp: 1.512 ± 0.273
1.84AsnGlu: 1.84 ± 0.311
1.052AsnPhe: 1.052 ± 0.219
3.549AsnGly: 3.549 ± 0.422
0.526AsnHis: 0.526 ± 0.149
1.314AsnIle: 1.314 ± 0.272
1.183AsnLys: 1.183 ± 0.307
3.023AsnLeu: 3.023 ± 0.477
0.46AsnMet: 0.46 ± 0.167
0.723AsnAsn: 0.723 ± 0.244
1.709AsnPro: 1.709 ± 0.343
1.117AsnGln: 1.117 ± 0.239
2.103AsnArg: 2.103 ± 0.301
0.986AsnSer: 0.986 ± 0.211
2.103AsnThr: 2.103 ± 0.384
1.512AsnVal: 1.512 ± 0.344
0.657AsnTrp: 0.657 ± 0.185
0.723AsnTyr: 0.723 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
5.192ProAla: 5.192 ± 0.672
0.526ProCys: 0.526 ± 0.205
3.221ProAsp: 3.221 ± 0.416
4.141ProGlu: 4.141 ± 0.468
1.446ProPhe: 1.446 ± 0.333
3.943ProGly: 3.943 ± 0.511
0.92ProHis: 0.92 ± 0.211
2.3ProIle: 2.3 ± 0.572
3.023ProLys: 3.023 ± 0.576
3.155ProLeu: 3.155 ± 0.517
0.986ProMet: 0.986 ± 0.213
1.314ProAsn: 1.314 ± 0.273
2.3ProPro: 2.3 ± 0.446
2.432ProGln: 2.432 ± 0.36
2.366ProArg: 2.366 ± 0.354
3.418ProSer: 3.418 ± 0.65
2.958ProThr: 2.958 ± 0.586
4.141ProVal: 4.141 ± 0.476
0.592ProTrp: 0.592 ± 0.173
0.592ProTyr: 0.592 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
4.535GlnAla: 4.535 ± 0.45
0.131GlnCys: 0.131 ± 0.077
1.577GlnAsp: 1.577 ± 0.351
2.169GlnGlu: 2.169 ± 0.366
1.446GlnPhe: 1.446 ± 0.251
1.775GlnGly: 1.775 ± 0.378
0.329GlnHis: 0.329 ± 0.146
1.906GlnIle: 1.906 ± 0.492
1.512GlnLys: 1.512 ± 0.266
2.695GlnLeu: 2.695 ± 0.434
0.854GlnMet: 0.854 ± 0.211
1.052GlnAsn: 1.052 ± 0.256
1.643GlnPro: 1.643 ± 0.311
0.854GlnGln: 0.854 ± 0.185
2.695GlnArg: 2.695 ± 0.412
2.103GlnSer: 2.103 ± 0.437
1.775GlnThr: 1.775 ± 0.35
2.432GlnVal: 2.432 ± 0.298
0.592GlnTrp: 0.592 ± 0.223
0.854GlnTyr: 0.854 ± 0.258
0.0GlnXaa: 0.0 ± 0.0
Arg
5.389ArgAla: 5.389 ± 0.63
0.526ArgCys: 0.526 ± 0.253
3.943ArgAsp: 3.943 ± 0.518
4.929ArgGlu: 4.929 ± 0.629
3.286ArgPhe: 3.286 ± 0.304
4.404ArgGly: 4.404 ± 0.515
1.249ArgHis: 1.249 ± 0.304
2.037ArgIle: 2.037 ± 0.331
3.418ArgLys: 3.418 ± 0.528
5.324ArgLeu: 5.324 ± 0.575
1.775ArgMet: 1.775 ± 0.335
1.709ArgAsn: 1.709 ± 0.292
2.103ArgPro: 2.103 ± 0.372
2.695ArgGln: 2.695 ± 0.446
5.784ArgArg: 5.784 ± 0.81
3.878ArgSer: 3.878 ± 0.652
3.943ArgThr: 3.943 ± 0.493
5.127ArgVal: 5.127 ± 0.66
1.183ArgTrp: 1.183 ± 0.262
1.972ArgTyr: 1.972 ± 0.378
0.0ArgXaa: 0.0 ± 0.0
Ser
5.127SerAla: 5.127 ± 0.595
0.131SerCys: 0.131 ± 0.09
3.352SerAsp: 3.352 ± 0.551
4.338SerGlu: 4.338 ± 0.519
2.037SerPhe: 2.037 ± 0.358
5.258SerGly: 5.258 ± 0.744
1.052SerHis: 1.052 ± 0.311
2.892SerIle: 2.892 ± 0.393
1.972SerLys: 1.972 ± 0.345
6.047SerLeu: 6.047 ± 0.741
1.052SerMet: 1.052 ± 0.237
1.643SerAsn: 1.643 ± 0.331
2.629SerPro: 2.629 ± 0.313
1.775SerGln: 1.775 ± 0.322
4.075SerArg: 4.075 ± 0.496
3.878SerSer: 3.878 ± 0.79
4.338SerThr: 4.338 ± 0.828
4.666SerVal: 4.666 ± 0.462
1.314SerTrp: 1.314 ± 0.285
1.906SerTyr: 1.906 ± 0.324
0.0SerXaa: 0.0 ± 0.0
Thr
6.638ThrAla: 6.638 ± 0.811
0.329ThrCys: 0.329 ± 0.139
3.286ThrAsp: 3.286 ± 0.579
4.535ThrGlu: 4.535 ± 0.567
2.695ThrPhe: 2.695 ± 0.644
6.244ThrGly: 6.244 ± 1.207
2.037ThrHis: 2.037 ± 0.437
2.432ThrIle: 2.432 ± 0.464
2.695ThrLys: 2.695 ± 0.538
5.389ThrLeu: 5.389 ± 0.711
1.052ThrMet: 1.052 ± 0.25
1.972ThrAsn: 1.972 ± 0.359
3.943ThrPro: 3.943 ± 0.716
1.709ThrGln: 1.709 ± 0.322
3.286ThrArg: 3.286 ± 0.598
3.878ThrSer: 3.878 ± 0.691
4.601ThrThr: 4.601 ± 0.656
5.061ThrVal: 5.061 ± 0.827
0.986ThrTrp: 0.986 ± 0.249
2.366ThrTyr: 2.366 ± 0.433
0.0ThrXaa: 0.0 ± 0.0
Val
8.281ValAla: 8.281 ± 0.618
0.526ValCys: 0.526 ± 0.195
4.009ValAsp: 4.009 ± 0.44
5.521ValGlu: 5.521 ± 0.567
1.972ValPhe: 1.972 ± 0.316
5.587ValGly: 5.587 ± 0.652
1.709ValHis: 1.709 ± 0.294
2.958ValIle: 2.958 ± 0.476
3.943ValLys: 3.943 ± 0.575
6.638ValLeu: 6.638 ± 0.689
1.446ValMet: 1.446 ± 0.314
2.169ValAsn: 2.169 ± 0.493
3.352ValPro: 3.352 ± 0.378
2.169ValGln: 2.169 ± 0.432
4.666ValArg: 4.666 ± 0.527
4.666ValSer: 4.666 ± 0.491
5.587ValThr: 5.587 ± 0.508
4.995ValVal: 4.995 ± 0.654
1.512ValTrp: 1.512 ± 0.328
1.314ValTyr: 1.314 ± 0.294
0.0ValXaa: 0.0 ± 0.0
Trp
1.906TrpAla: 1.906 ± 0.378
0.263TrpCys: 0.263 ± 0.132
1.577TrpAsp: 1.577 ± 0.278
1.38TrpGlu: 1.38 ± 0.338
0.46TrpPhe: 0.46 ± 0.16
1.577TrpGly: 1.577 ± 0.255
0.263TrpHis: 0.263 ± 0.15
0.92TrpIle: 0.92 ± 0.276
0.92TrpLys: 0.92 ± 0.22
1.314TrpLeu: 1.314 ± 0.29
0.394TrpMet: 0.394 ± 0.148
0.854TrpAsn: 0.854 ± 0.208
0.329TrpPro: 0.329 ± 0.119
0.657TrpGln: 0.657 ± 0.201
1.249TrpArg: 1.249 ± 0.284
1.512TrpSer: 1.512 ± 0.354
1.38TrpThr: 1.38 ± 0.284
1.446TrpVal: 1.446 ± 0.297
0.131TrpTrp: 0.131 ± 0.124
0.263TrpTyr: 0.263 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.155TyrAla: 3.155 ± 0.431
0.131TyrCys: 0.131 ± 0.07
2.103TyrAsp: 2.103 ± 0.418
2.498TyrGlu: 2.498 ± 0.498
0.657TyrPhe: 0.657 ± 0.179
2.958TyrGly: 2.958 ± 0.474
0.46TyrHis: 0.46 ± 0.175
0.92TyrIle: 0.92 ± 0.233
0.723TyrLys: 0.723 ± 0.251
1.906TyrLeu: 1.906 ± 0.254
0.657TyrMet: 0.657 ± 0.195
0.854TyrAsn: 0.854 ± 0.227
1.314TyrPro: 1.314 ± 0.34
0.657TyrGln: 0.657 ± 0.222
2.235TyrArg: 2.235 ± 0.47
1.906TyrSer: 1.906 ± 0.382
1.709TyrThr: 1.709 ± 0.291
1.249TyrVal: 1.249 ± 0.284
0.526TyrTrp: 0.526 ± 0.226
0.657TyrTyr: 0.657 ± 0.167
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (15216 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski