Amino acid dipepetide frequency for Streptomyces phage Xkcd426

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.602AlaAla: 20.602 ± 1.594
1.23AlaCys: 1.23 ± 0.278
7.892AlaAsp: 7.892 ± 0.886
10.198AlaGlu: 10.198 ± 1.095
3.331AlaPhe: 3.331 ± 0.422
11.428AlaGly: 11.428 ± 0.963
1.896AlaHis: 1.896 ± 0.282
4.049AlaIle: 4.049 ± 0.63
3.69AlaLys: 3.69 ± 0.526
9.225AlaLeu: 9.225 ± 1.265
2.972AlaMet: 2.972 ± 0.46
2.921AlaAsn: 2.921 ± 0.412
6.457AlaPro: 6.457 ± 0.782
4.356AlaGln: 4.356 ± 0.469
8.507AlaArg: 8.507 ± 0.963
7.123AlaSer: 7.123 ± 0.62
9.173AlaThr: 9.173 ± 0.728
9.532AlaVal: 9.532 ± 0.716
1.332AlaTrp: 1.332 ± 0.242
2.511AlaTyr: 2.511 ± 0.369
0.0AlaXaa: 0.0 ± 0.0
Cys
2.204CysAla: 2.204 ± 0.479
0.769CysCys: 0.769 ± 0.221
1.281CysAsp: 1.281 ± 0.4
0.564CysGlu: 0.564 ± 0.199
0.256CysPhe: 0.256 ± 0.099
1.896CysGly: 1.896 ± 0.486
0.512CysHis: 0.512 ± 0.157
0.666CysIle: 0.666 ± 0.166
0.256CysLys: 0.256 ± 0.128
0.769CysLeu: 0.769 ± 0.187
0.512CysMet: 0.512 ± 0.18
0.769CysAsn: 0.769 ± 0.297
1.23CysPro: 1.23 ± 0.287
0.461CysGln: 0.461 ± 0.141
1.025CysArg: 1.025 ± 0.271
0.615CysSer: 0.615 ± 0.174
1.127CysThr: 1.127 ± 0.305
1.23CysVal: 1.23 ± 0.313
0.256CysTrp: 0.256 ± 0.116
0.41CysTyr: 0.41 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
7.533AspAla: 7.533 ± 0.668
1.332AspCys: 1.332 ± 0.368
4.612AspAsp: 4.612 ± 0.642
4.202AspGlu: 4.202 ± 0.554
1.845AspPhe: 1.845 ± 0.273
7.431AspGly: 7.431 ± 0.691
1.281AspHis: 1.281 ± 0.275
2.46AspIle: 2.46 ± 0.498
1.076AspLys: 1.076 ± 0.238
5.689AspLeu: 5.689 ± 0.558
1.999AspMet: 1.999 ± 0.319
1.384AspAsn: 1.384 ± 0.271
3.997AspPro: 3.997 ± 0.582
2.255AspGln: 2.255 ± 0.295
4.561AspArg: 4.561 ± 0.461
3.331AspSer: 3.331 ± 0.456
3.024AspThr: 3.024 ± 0.479
5.586AspVal: 5.586 ± 0.457
0.974AspTrp: 0.974 ± 0.229
1.076AspTyr: 1.076 ± 0.229
0.0AspXaa: 0.0 ± 0.0
Glu
7.585GluAla: 7.585 ± 0.757
0.974GluCys: 0.974 ± 0.225
2.665GluAsp: 2.665 ± 0.471
2.562GluGlu: 2.562 ± 0.465
1.23GluPhe: 1.23 ± 0.28
4.151GluGly: 4.151 ± 0.595
0.41GluHis: 0.41 ± 0.115
1.742GluIle: 1.742 ± 0.381
0.512GluLys: 0.512 ± 0.183
5.637GluLeu: 5.637 ± 0.548
1.332GluMet: 1.332 ± 0.264
0.615GluAsn: 0.615 ± 0.165
2.357GluPro: 2.357 ± 0.386
2.05GluGln: 2.05 ± 0.376
4.202GluArg: 4.202 ± 0.602
1.332GluSer: 1.332 ± 0.263
3.792GluThr: 3.792 ± 0.454
3.587GluVal: 3.587 ± 0.446
0.769GluTrp: 0.769 ± 0.224
1.332GluTyr: 1.332 ± 0.267
0.0GluXaa: 0.0 ± 0.0
Phe
2.665PheAla: 2.665 ± 0.378
0.307PheCys: 0.307 ± 0.123
2.46PheAsp: 2.46 ± 0.376
1.486PheGlu: 1.486 ± 0.281
0.615PhePhe: 0.615 ± 0.225
2.87PheGly: 2.87 ± 0.378
0.461PheHis: 0.461 ± 0.144
0.82PheIle: 0.82 ± 0.22
0.359PheLys: 0.359 ± 0.119
2.409PheLeu: 2.409 ± 0.335
0.512PheMet: 0.512 ± 0.194
1.589PheAsn: 1.589 ± 0.342
1.537PhePro: 1.537 ± 0.313
0.974PheGln: 0.974 ± 0.196
1.281PheArg: 1.281 ± 0.258
1.742PheSer: 1.742 ± 0.307
2.46PheThr: 2.46 ± 0.403
1.794PheVal: 1.794 ± 0.337
0.205PheTrp: 0.205 ± 0.112
0.307PheTyr: 0.307 ± 0.139
0.0PheXaa: 0.0 ± 0.0
Gly
10.096GlyAla: 10.096 ± 0.97
1.947GlyCys: 1.947 ± 0.475
5.125GlyAsp: 5.125 ± 0.646
3.792GlyGlu: 3.792 ± 0.368
2.921GlyPhe: 2.921 ± 0.378
10.762GlyGly: 10.762 ± 2.293
1.999GlyHis: 1.999 ± 0.344
3.639GlyIle: 3.639 ± 0.408
3.177GlyLys: 3.177 ± 0.545
6.816GlyLeu: 6.816 ± 0.771
1.845GlyMet: 1.845 ± 0.326
2.972GlyAsn: 2.972 ± 0.453
4.869GlyPro: 4.869 ± 0.559
3.639GlyGln: 3.639 ± 0.442
5.894GlyArg: 5.894 ± 0.58
4.817GlySer: 4.817 ± 0.552
8.405GlyThr: 8.405 ± 1.039
6.662GlyVal: 6.662 ± 0.435
2.152GlyTrp: 2.152 ± 0.313
2.255GlyTyr: 2.255 ± 0.259
0.0GlyXaa: 0.0 ± 0.0
His
1.794HisAla: 1.794 ± 0.317
0.102HisCys: 0.102 ± 0.073
1.23HisAsp: 1.23 ± 0.235
0.359HisGlu: 0.359 ± 0.122
0.307HisPhe: 0.307 ± 0.111
1.794HisGly: 1.794 ± 0.369
0.666HisHis: 0.666 ± 0.211
0.41HisIle: 0.41 ± 0.139
0.41HisLys: 0.41 ± 0.197
1.589HisLeu: 1.589 ± 0.293
0.82HisMet: 0.82 ± 0.254
0.769HisAsn: 0.769 ± 0.17
1.076HisPro: 1.076 ± 0.224
0.769HisGln: 0.769 ± 0.214
1.435HisArg: 1.435 ± 0.219
1.025HisSer: 1.025 ± 0.209
1.486HisThr: 1.486 ± 0.354
1.64HisVal: 1.64 ± 0.336
0.615HisTrp: 0.615 ± 0.188
0.461HisTyr: 0.461 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
4.151IleAla: 4.151 ± 0.687
0.359IleCys: 0.359 ± 0.141
1.947IleAsp: 1.947 ± 0.303
2.972IleGlu: 2.972 ± 0.451
1.076IlePhe: 1.076 ± 0.179
3.177IleGly: 3.177 ± 0.455
0.666IleHis: 0.666 ± 0.186
1.23IleIle: 1.23 ± 0.33
0.769IleLys: 0.769 ± 0.22
2.87IleLeu: 2.87 ± 0.392
0.717IleMet: 0.717 ± 0.192
1.384IleAsn: 1.384 ± 0.253
1.845IlePro: 1.845 ± 0.287
1.742IleGln: 1.742 ± 0.336
2.511IleArg: 2.511 ± 0.341
1.537IleSer: 1.537 ± 0.282
3.434IleThr: 3.434 ± 0.548
3.229IleVal: 3.229 ± 0.382
0.41IleTrp: 0.41 ± 0.143
0.512IleTyr: 0.512 ± 0.181
0.0IleXaa: 0.0 ± 0.0
Lys
3.997LysAla: 3.997 ± 0.651
0.359LysCys: 0.359 ± 0.184
1.845LysAsp: 1.845 ± 0.353
0.974LysGlu: 0.974 ± 0.266
0.512LysPhe: 0.512 ± 0.181
2.46LysGly: 2.46 ± 0.362
0.871LysHis: 0.871 ± 0.234
0.922LysIle: 0.922 ± 0.225
1.076LysLys: 1.076 ± 0.223
2.972LysLeu: 2.972 ± 0.429
0.82LysMet: 0.82 ± 0.272
0.512LysAsn: 0.512 ± 0.132
2.409LysPro: 2.409 ± 0.44
1.025LysGln: 1.025 ± 0.285
1.999LysArg: 1.999 ± 0.439
1.435LysSer: 1.435 ± 0.291
1.794LysThr: 1.794 ± 0.329
1.999LysVal: 1.999 ± 0.366
0.359LysTrp: 0.359 ± 0.123
0.461LysTyr: 0.461 ± 0.151
0.0LysXaa: 0.0 ± 0.0
Leu
10.557LeuAla: 10.557 ± 0.893
1.281LeuCys: 1.281 ± 0.372
5.227LeuAsp: 5.227 ± 0.562
2.87LeuGlu: 2.87 ± 0.376
1.896LeuPhe: 1.896 ± 0.336
7.38LeuGly: 7.38 ± 0.812
1.64LeuHis: 1.64 ± 0.26
2.255LeuIle: 2.255 ± 0.36
2.255LeuLys: 2.255 ± 0.39
6.201LeuLeu: 6.201 ± 0.727
1.025LeuMet: 1.025 ± 0.206
3.28LeuAsn: 3.28 ± 0.378
4.459LeuPro: 4.459 ± 0.418
1.486LeuGln: 1.486 ± 0.359
6.15LeuArg: 6.15 ± 0.605
4.51LeuSer: 4.51 ± 0.437
7.585LeuThr: 7.585 ± 0.696
6.918LeuVal: 6.918 ± 0.714
0.666LeuTrp: 0.666 ± 0.176
1.742LeuTyr: 1.742 ± 0.249
0.0LeuXaa: 0.0 ± 0.0
Met
3.587MetAla: 3.587 ± 0.389
0.0MetCys: 0.0 ± 0.0
1.589MetAsp: 1.589 ± 0.413
0.512MetGlu: 0.512 ± 0.172
0.359MetPhe: 0.359 ± 0.113
1.486MetGly: 1.486 ± 0.256
0.41MetHis: 0.41 ± 0.141
0.871MetIle: 0.871 ± 0.168
1.281MetLys: 1.281 ± 0.266
1.537MetLeu: 1.537 ± 0.394
0.256MetMet: 0.256 ± 0.116
0.974MetAsn: 0.974 ± 0.231
1.025MetPro: 1.025 ± 0.194
0.717MetGln: 0.717 ± 0.232
1.486MetArg: 1.486 ± 0.278
1.332MetSer: 1.332 ± 0.301
1.896MetThr: 1.896 ± 0.308
1.537MetVal: 1.537 ± 0.278
0.307MetTrp: 0.307 ± 0.128
0.307MetTyr: 0.307 ± 0.119
0.0MetXaa: 0.0 ± 0.0
Asn
4.049AsnAla: 4.049 ± 0.563
0.769AsnCys: 0.769 ± 0.267
1.486AsnAsp: 1.486 ± 0.271
0.974AsnGlu: 0.974 ± 0.268
0.871AsnPhe: 0.871 ± 0.226
3.587AsnGly: 3.587 ± 0.476
0.666AsnHis: 0.666 ± 0.208
1.332AsnIle: 1.332 ± 0.336
0.41AsnLys: 0.41 ± 0.128
1.999AsnLeu: 1.999 ± 0.362
0.41AsnMet: 0.41 ± 0.132
0.666AsnAsn: 0.666 ± 0.164
2.819AsnPro: 2.819 ± 0.382
1.742AsnGln: 1.742 ± 0.302
2.614AsnArg: 2.614 ± 0.391
1.23AsnSer: 1.23 ± 0.265
1.691AsnThr: 1.691 ± 0.257
2.511AsnVal: 2.511 ± 0.359
0.205AsnTrp: 0.205 ± 0.097
0.512AsnTyr: 0.512 ± 0.158
0.0AsnXaa: 0.0 ± 0.0
Pro
7.175ProAla: 7.175 ± 0.809
0.871ProCys: 0.871 ± 0.22
4.1ProAsp: 4.1 ± 0.483
3.485ProGlu: 3.485 ± 0.503
1.179ProPhe: 1.179 ± 0.252
6.406ProGly: 6.406 ± 0.724
0.922ProHis: 0.922 ± 0.24
2.05ProIle: 2.05 ± 0.357
2.101ProLys: 2.101 ± 0.468
3.895ProLeu: 3.895 ± 0.593
1.025ProMet: 1.025 ± 0.221
1.486ProAsn: 1.486 ± 0.332
2.972ProPro: 2.972 ± 0.55
1.794ProGln: 1.794 ± 0.31
2.87ProArg: 2.87 ± 0.505
3.126ProSer: 3.126 ± 0.417
3.997ProThr: 3.997 ± 0.531
6.047ProVal: 6.047 ± 0.494
1.435ProTrp: 1.435 ± 0.235
0.922ProTyr: 0.922 ± 0.222
0.0ProXaa: 0.0 ± 0.0
Gln
3.895GlnAla: 3.895 ± 0.635
0.615GlnCys: 0.615 ± 0.171
2.409GlnAsp: 2.409 ± 0.271
0.717GlnGlu: 0.717 ± 0.181
1.384GlnPhe: 1.384 ± 0.248
2.101GlnGly: 2.101 ± 0.332
0.974GlnHis: 0.974 ± 0.222
1.537GlnIle: 1.537 ± 0.354
0.615GlnLys: 0.615 ± 0.205
4.049GlnLeu: 4.049 ± 0.387
0.82GlnMet: 0.82 ± 0.181
0.82GlnAsn: 0.82 ± 0.218
2.767GlnPro: 2.767 ± 0.421
1.435GlnGln: 1.435 ± 0.286
2.819GlnArg: 2.819 ± 0.441
1.486GlnSer: 1.486 ± 0.295
2.409GlnThr: 2.409 ± 0.331
2.357GlnVal: 2.357 ± 0.338
0.512GlnTrp: 0.512 ± 0.172
0.717GlnTyr: 0.717 ± 0.197
0.0GlnXaa: 0.0 ± 0.0
Arg
7.636ArgAla: 7.636 ± 0.887
1.435ArgCys: 1.435 ± 0.426
4.612ArgAsp: 4.612 ± 0.602
2.921ArgGlu: 2.921 ± 0.472
2.101ArgPhe: 2.101 ± 0.392
4.561ArgGly: 4.561 ± 0.422
1.64ArgHis: 1.64 ± 0.286
3.69ArgIle: 3.69 ± 0.486
2.972ArgLys: 2.972 ± 0.441
5.227ArgLeu: 5.227 ± 0.531
2.255ArgMet: 2.255 ± 0.388
1.896ArgAsn: 1.896 ± 0.312
3.587ArgPro: 3.587 ± 0.474
1.999ArgGln: 1.999 ± 0.329
5.535ArgArg: 5.535 ± 0.665
2.204ArgSer: 2.204 ± 0.32
4.151ArgThr: 4.151 ± 0.482
5.842ArgVal: 5.842 ± 0.554
0.922ArgTrp: 0.922 ± 0.213
1.845ArgTyr: 1.845 ± 0.347
0.0ArgXaa: 0.0 ± 0.0
Ser
6.56SerAla: 6.56 ± 0.603
1.025SerCys: 1.025 ± 0.306
2.921SerAsp: 2.921 ± 0.367
2.152SerGlu: 2.152 ± 0.331
1.845SerPhe: 1.845 ± 0.332
6.15SerGly: 6.15 ± 0.665
0.717SerHis: 0.717 ± 0.202
1.537SerIle: 1.537 ± 0.238
1.845SerLys: 1.845 ± 0.33
3.485SerLeu: 3.485 ± 0.437
1.23SerMet: 1.23 ± 0.227
2.255SerAsn: 2.255 ± 0.369
2.409SerPro: 2.409 ± 0.335
1.384SerGln: 1.384 ± 0.314
2.87SerArg: 2.87 ± 0.445
2.921SerSer: 2.921 ± 0.355
3.69SerThr: 3.69 ± 0.402
3.485SerVal: 3.485 ± 0.285
1.076SerTrp: 1.076 ± 0.239
1.384SerTyr: 1.384 ± 0.264
0.0SerXaa: 0.0 ± 0.0
Thr
9.071ThrAla: 9.071 ± 0.693
1.435ThrCys: 1.435 ± 0.409
4.869ThrAsp: 4.869 ± 0.509
4.664ThrGlu: 4.664 ± 0.422
2.819ThrPhe: 2.819 ± 0.389
8.2ThrGly: 8.2 ± 0.968
0.666ThrHis: 0.666 ± 0.183
2.972ThrIle: 2.972 ± 0.379
2.204ThrLys: 2.204 ± 0.395
6.457ThrLeu: 6.457 ± 0.612
0.974ThrMet: 0.974 ± 0.208
2.101ThrAsn: 2.101 ± 0.384
5.33ThrPro: 5.33 ± 0.623
2.204ThrGln: 2.204 ± 0.352
3.331ThrArg: 3.331 ± 0.396
3.946ThrSer: 3.946 ± 0.406
5.996ThrThr: 5.996 ± 0.675
7.277ThrVal: 7.277 ± 0.729
1.23ThrTrp: 1.23 ± 0.303
1.845ThrTyr: 1.845 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
11.838ValAla: 11.838 ± 0.839
1.384ValCys: 1.384 ± 0.283
6.611ValAsp: 6.611 ± 0.663
2.409ValGlu: 2.409 ± 0.313
1.64ValPhe: 1.64 ± 0.231
4.356ValGly: 4.356 ± 0.513
1.64ValHis: 1.64 ± 0.316
3.331ValIle: 3.331 ± 0.398
2.716ValLys: 2.716 ± 0.43
5.586ValLeu: 5.586 ± 0.557
1.281ValMet: 1.281 ± 0.221
2.716ValAsn: 2.716 ± 0.378
4.92ValPro: 4.92 ± 0.549
3.024ValGln: 3.024 ± 0.505
5.022ValArg: 5.022 ± 0.456
4.971ValSer: 4.971 ± 0.461
7.892ValThr: 7.892 ± 0.83
6.355ValVal: 6.355 ± 0.625
1.486ValTrp: 1.486 ± 0.275
1.23ValTyr: 1.23 ± 0.241
0.0ValXaa: 0.0 ± 0.0
Trp
1.537TrpAla: 1.537 ± 0.297
0.307TrpCys: 0.307 ± 0.12
1.025TrpAsp: 1.025 ± 0.295
0.154TrpGlu: 0.154 ± 0.088
0.359TrpPhe: 0.359 ± 0.177
0.922TrpGly: 0.922 ± 0.228
0.41TrpHis: 0.41 ± 0.145
0.359TrpIle: 0.359 ± 0.138
0.615TrpLys: 0.615 ± 0.186
1.691TrpLeu: 1.691 ± 0.271
0.205TrpMet: 0.205 ± 0.115
0.512TrpAsn: 0.512 ± 0.154
0.871TrpPro: 0.871 ± 0.194
0.666TrpGln: 0.666 ± 0.13
0.769TrpArg: 0.769 ± 0.206
1.23TrpSer: 1.23 ± 0.248
1.742TrpThr: 1.742 ± 0.31
1.332TrpVal: 1.332 ± 0.27
0.307TrpTrp: 0.307 ± 0.124
0.461TrpTyr: 0.461 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.896TyrAla: 1.896 ± 0.315
0.307TyrCys: 0.307 ± 0.125
1.742TyrAsp: 1.742 ± 0.306
0.871TyrGlu: 0.871 ± 0.202
0.307TyrPhe: 0.307 ± 0.116
2.665TyrGly: 2.665 ± 0.507
0.307TyrHis: 0.307 ± 0.116
0.666TyrIle: 0.666 ± 0.17
0.461TyrLys: 0.461 ± 0.128
1.281TyrLeu: 1.281 ± 0.279
0.41TyrMet: 0.41 ± 0.151
0.922TyrAsn: 0.922 ± 0.17
0.922TyrPro: 0.922 ± 0.202
0.717TyrGln: 0.717 ± 0.177
2.204TyrArg: 2.204 ± 0.364
1.025TyrSer: 1.025 ± 0.214
1.845TyrThr: 1.845 ± 0.332
1.486TyrVal: 1.486 ± 0.325
0.256TyrTrp: 0.256 ± 0.115
0.102TyrTyr: 0.102 ± 0.068
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (19514 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski