Hubei sobemo-like virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEY4|A0A1L3KEY4_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 15 OX=1923200 PE=4 SV=1
MM1 pKa = 7.26VSASTGRR8 pKa = 11.84VEE10 pKa = 5.55FSVDD14 pKa = 3.12TQRR17 pKa = 11.84QLFDD21 pKa = 3.93AALEE25 pKa = 4.18DD26 pKa = 4.47LNMTSSVGKK35 pKa = 9.79CQMDD39 pKa = 3.66RR40 pKa = 11.84YY41 pKa = 10.08GATIGVALGYY51 pKa = 10.84DD52 pKa = 4.37PIAGCNQEE60 pKa = 5.06RR61 pKa = 11.84VDD63 pKa = 4.55FLYY66 pKa = 10.96HH67 pKa = 6.13LVSEE71 pKa = 4.78RR72 pKa = 11.84LEE74 pKa = 4.01EE75 pKa = 4.01MRR77 pKa = 11.84GGRR80 pKa = 11.84LVSDD84 pKa = 5.2PINLFVKK91 pKa = 10.17QEE93 pKa = 3.75PHH95 pKa = 5.77KK96 pKa = 10.2QSKK99 pKa = 9.2IRR101 pKa = 11.84EE102 pKa = 3.88GRR104 pKa = 11.84YY105 pKa = 9.33RR106 pKa = 11.84LISAVSLVDD115 pKa = 3.17TMIDD119 pKa = 3.12RR120 pKa = 11.84MIFGHH125 pKa = 6.64IKK127 pKa = 9.22QDD129 pKa = 2.93AWQDD133 pKa = 3.71SPVLIGWTPRR143 pKa = 11.84QGGYY147 pKa = 8.74RR148 pKa = 11.84WLWEE152 pKa = 3.88RR153 pKa = 11.84LDD155 pKa = 3.55GGKK158 pKa = 9.85RR159 pKa = 11.84LEE161 pKa = 3.94IDD163 pKa = 2.76KK164 pKa = 10.92RR165 pKa = 11.84AWDD168 pKa = 3.47WSMQPWVVRR177 pKa = 11.84GCLEE181 pKa = 3.86VLEE184 pKa = 4.33QLSLHH189 pKa = 6.35HH190 pKa = 6.32TDD192 pKa = 2.94EE193 pKa = 4.21WLKK196 pKa = 10.27LARR199 pKa = 11.84SRR201 pKa = 11.84FEE203 pKa = 3.71ALFYY207 pKa = 10.58RR208 pKa = 11.84ARR210 pKa = 11.84FDD212 pKa = 3.89GAGLTFDD219 pKa = 3.41QAEE222 pKa = 4.09PGIMKK227 pKa = 10.04SGCFLTIFANSLSQLLLHH245 pKa = 6.28FVAVRR250 pKa = 11.84RR251 pKa = 11.84SRR253 pKa = 11.84LGNLIEE259 pKa = 5.57APFCMGDD266 pKa = 3.84DD267 pKa = 4.38TIQRR271 pKa = 11.84CWASEE276 pKa = 4.1DD277 pKa = 3.61QVDD280 pKa = 3.99EE281 pKa = 4.18YY282 pKa = 11.65VRR284 pKa = 11.84QLEE287 pKa = 4.15EE288 pKa = 4.12AGCVVGDD295 pKa = 4.91CEE297 pKa = 4.84LLPAGSDD304 pKa = 3.57FQFCGFRR311 pKa = 11.84ITKK314 pKa = 10.06NKK316 pKa = 9.19VVPAYY321 pKa = 7.31TAKK324 pKa = 10.35HH325 pKa = 5.75CFVLQHH331 pKa = 6.72LEE333 pKa = 3.97DD334 pKa = 4.05DD335 pKa = 4.37TAVEE339 pKa = 4.41TLDD342 pKa = 5.5SYY344 pKa = 9.71QQDD347 pKa = 4.2YY348 pKa = 10.97VFEE351 pKa = 3.96PHH353 pKa = 6.85IYY355 pKa = 10.34GHH357 pKa = 6.57IADD360 pKa = 4.81GLLEE364 pKa = 4.08IRR366 pKa = 11.84PNALRR371 pKa = 11.84SKK373 pKa = 10.38GKK375 pKa = 8.13MRR377 pKa = 11.84RR378 pKa = 11.84FLRR381 pKa = 11.84GDD383 pKa = 3.31EE384 pKa = 4.08

Molecular weight: 43.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEY4|A0A1L3KEY4_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 15 OX=1923200 PE=4 SV=1
MM1 pKa = 7.21LQAGSQRR8 pKa = 11.84EE9 pKa = 4.26HH10 pKa = 6.9VLLAVLIAVTVGFFWLDD27 pKa = 2.75VHH29 pKa = 6.18RR30 pKa = 11.84QGVRR34 pKa = 11.84AEE36 pKa = 4.26LVSEE40 pKa = 4.27SMKK43 pKa = 10.9DD44 pKa = 3.38LNTLRR49 pKa = 11.84RR50 pKa = 11.84ILEE53 pKa = 4.06LNQRR57 pKa = 11.84LLRR60 pKa = 11.84NCQSTVFHH68 pKa = 6.97LKK70 pKa = 8.89QEE72 pKa = 4.19RR73 pKa = 11.84AQMDD77 pKa = 3.72RR78 pKa = 11.84VVARR82 pKa = 11.84LAAARR87 pKa = 11.84EE88 pKa = 4.44EE89 pKa = 4.41ACSPRR94 pKa = 11.84AADD97 pKa = 3.74RR98 pKa = 11.84VVSAAGDD105 pKa = 3.53AASWVAGAAAFGFALAWSAVAAYY128 pKa = 6.34WTLAFGVVGVVCMSYY143 pKa = 10.59VLWRR147 pKa = 11.84LLCLVYY153 pKa = 10.1RR154 pKa = 11.84WVRR157 pKa = 11.84EE158 pKa = 4.19RR159 pKa = 11.84IWNWRR164 pKa = 11.84APMVVEE170 pKa = 4.25EE171 pKa = 4.55QPLDD175 pKa = 3.98GPTLGCATLQWPGVEE190 pKa = 4.17IQPEE194 pKa = 4.79RR195 pKa = 11.84YY196 pKa = 8.81QAGSDD201 pKa = 3.56PHH203 pKa = 6.55PVSLKK208 pKa = 10.39DD209 pKa = 3.61LPPFQVAVYY218 pKa = 8.79TKK220 pKa = 10.62GAGKK224 pKa = 8.49TEE226 pKa = 4.53TYY228 pKa = 9.67FQGYY232 pKa = 9.11ALNIEE237 pKa = 5.17GYY239 pKa = 10.7LVMPLHH245 pKa = 5.99VKK247 pKa = 10.03EE248 pKa = 4.56PEE250 pKa = 4.46GVPHH254 pKa = 7.07DD255 pKa = 3.9HH256 pKa = 7.17LVLQRR261 pKa = 11.84YY262 pKa = 9.04VGDD265 pKa = 3.86EE266 pKa = 3.92LRR268 pKa = 11.84QTTLGEE274 pKa = 4.26TITWMEE280 pKa = 4.32LAQDD284 pKa = 3.78AVAADD289 pKa = 3.89MSTMKK294 pKa = 10.13FDD296 pKa = 3.59KK297 pKa = 11.19GNLEE301 pKa = 4.28SYY303 pKa = 10.67LGLAKK308 pKa = 10.62AKK310 pKa = 10.24VADD313 pKa = 3.94VTEE316 pKa = 4.37MTHH319 pKa = 6.45CSIVGGFRR327 pKa = 11.84PVARR331 pKa = 11.84TMGAVRR337 pKa = 11.84LLSEE341 pKa = 4.58FFGQVQYY348 pKa = 11.11TGTTFPGYY356 pKa = 10.15SGAAYY361 pKa = 9.62YY362 pKa = 10.51VNKK365 pKa = 9.1TVYY368 pKa = 10.7GMHH371 pKa = 6.24TSAWHH376 pKa = 5.11TMNVGYY382 pKa = 10.02SAAYY386 pKa = 9.04LASILRR392 pKa = 11.84KK393 pKa = 9.6RR394 pKa = 11.84RR395 pKa = 11.84GQKK398 pKa = 9.75EE399 pKa = 3.99SSEE402 pKa = 4.43DD403 pKa = 3.49FLFQRR408 pKa = 11.84MTKK411 pKa = 8.8QKK413 pKa = 10.3KK414 pKa = 8.3ILYY417 pKa = 7.02RR418 pKa = 11.84TSPGDD423 pKa = 3.49PEE425 pKa = 4.06EE426 pKa = 5.51VIMKK430 pKa = 9.99VEE432 pKa = 3.8GRR434 pKa = 11.84FYY436 pKa = 10.71TFARR440 pKa = 11.84DD441 pKa = 3.59EE442 pKa = 4.33IPRR445 pKa = 11.84SVWDD449 pKa = 3.84RR450 pKa = 11.84MVSADD455 pKa = 3.82DD456 pKa = 3.72VQGSGEE462 pKa = 4.29SYY464 pKa = 10.84NDD466 pKa = 3.14EE467 pKa = 4.09GCFLGRR473 pKa = 11.84RR474 pKa = 11.84PAQSQSLGQMGQLKK488 pKa = 10.25RR489 pKa = 11.84MPDD492 pKa = 3.17NRR494 pKa = 11.84SPGVSVSQHH503 pKa = 4.53GQQTIPNVAAGLSGQTPEE521 pKa = 4.18RR522 pKa = 11.84TSGSSSAKK530 pKa = 9.17NAEE533 pKa = 4.15GKK535 pKa = 9.21PEE537 pKa = 3.87NMVSQEE543 pKa = 4.2RR544 pKa = 11.84IVVQSPPPSSGTSSITEE561 pKa = 3.72QSATGSKK568 pKa = 9.77SRR570 pKa = 11.84RR571 pKa = 11.84NKK573 pKa = 10.12SSKK576 pKa = 10.54KK577 pKa = 9.12PP578 pKa = 3.44

Molecular weight: 63.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

962

384

578

481.0

53.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.316 ± 1.131

1.767 ± 0.524

5.301 ± 1.41

6.341 ± 0.269

3.846 ± 0.69

8.004 ± 0.12

2.183 ± 0.264

3.326 ± 0.853

3.95 ± 0.191

8.94 ± 0.925

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.015 ± 0.257

2.183 ± 0.226

4.158 ± 0.647

5.509 ± 0.189

7.588 ± 0.467

7.173 ± 0.904

4.782 ± 0.712

8.212 ± 0.903

2.183 ± 0.101

3.222 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski