Rickettsiales bacterium Ac37b

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; unclassified Rickettsiales

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077FIX2|A0A077FIX2_9RICK Uncharacterized protein OS=Rickettsiales bacterium Ac37b OX=1528098 GN=NOVO_05465 PE=4 SV=1
MM1 pKa = 7.16IAVMTYY7 pKa = 10.04LKK9 pKa = 10.15WLFYY13 pKa = 10.29PILALFITQPTFAAPIWTTSFGSQGTGDD41 pKa = 3.81GQFDD45 pKa = 4.33NPTGITTLPTGEE57 pKa = 4.37IYY59 pKa = 11.06VVDD62 pKa = 4.3GNRR65 pKa = 11.84NDD67 pKa = 3.57VQIFNPDD74 pKa = 3.0TTFSSKK80 pKa = 10.42FGSPGNGNGEE90 pKa = 4.21FNLPISIFRR99 pKa = 11.84SPSTGLLYY107 pKa = 10.45ISDD110 pKa = 4.12NFNHH114 pKa = 6.61RR115 pKa = 11.84VQIFNPDD122 pKa = 3.03GTYY125 pKa = 10.38SAQFGSFGDD134 pKa = 3.82GDD136 pKa = 4.42GQFMDD141 pKa = 4.48HH142 pKa = 6.71YY143 pKa = 9.75GITMDD148 pKa = 3.59TVNNKK153 pKa = 9.29FFVSDD158 pKa = 3.31VGNNNNPRR166 pKa = 11.84VQIFNDD172 pKa = 3.32DD173 pKa = 3.19GTYY176 pKa = 10.3FSQFGGFGTANGEE189 pKa = 4.43FYY191 pKa = 11.31SPMSLTFGNSQLYY204 pKa = 8.56VADD207 pKa = 3.73YY208 pKa = 10.13FLNNIQTFDD217 pKa = 3.43EE218 pKa = 4.42NANFLNKK225 pKa = 9.68FDD227 pKa = 3.96VSSIPSVYY235 pKa = 10.49LSRR238 pKa = 11.84PRR240 pKa = 11.84DD241 pKa = 3.45LAVDD245 pKa = 3.88NAHH248 pKa = 7.37DD249 pKa = 3.78MYY251 pKa = 11.71

Molecular weight: 27.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077FGQ8|A0A077FGQ8_9RICK Thioredoxin OS=Rickettsiales bacterium Ac37b OX=1528098 GN=trxA PE=3 SV=1
MM1 pKa = 7.82NYY3 pKa = 10.14RR4 pKa = 11.84IPKK7 pKa = 8.62TLDD10 pKa = 2.99NPVRR14 pKa = 11.84CLGIPIDD21 pKa = 3.81ALIVFMGIWGGFVVFDD37 pKa = 4.35KK38 pKa = 11.25GLFGIPVGIVVSMLFGKK55 pKa = 10.06FRR57 pKa = 11.84SRR59 pKa = 11.84SIVRR63 pKa = 11.84RR64 pKa = 11.84FIRR67 pKa = 11.84FLYY70 pKa = 9.37WYY72 pKa = 9.48LPSEE76 pKa = 4.21MNFIKK81 pKa = 10.44GVQGHH86 pKa = 4.92QRR88 pKa = 11.84KK89 pKa = 9.41LICKK93 pKa = 9.32

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1659

0

1659

520560

31

2243

313.8

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.289 ± 0.064

1.029 ± 0.023

4.878 ± 0.046

6.335 ± 0.067

4.199 ± 0.043

5.474 ± 0.067

2.168 ± 0.028

9.987 ± 0.072

7.732 ± 0.064

10.229 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.022

6.779 ± 0.067

3.317 ± 0.037

3.509 ± 0.037

3.409 ± 0.046

6.983 ± 0.045

5.19 ± 0.04

5.482 ± 0.046

0.805 ± 0.021

3.943 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski