Shahe sobemo-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEM1|A0A1L3KEM1_9VIRU RNA-directed RNA polymerase OS=Shahe sobemo-like virus 1 OX=1923454 PE=4 SV=1
MM1 pKa = 7.31GFIGIMASFFSLVVNVFFYY20 pKa = 9.87FSIFLAGALAVLMMEE35 pKa = 4.88GVQYY39 pKa = 10.28PFQNQIISAFVMYY52 pKa = 10.35IIVNGLLRR60 pKa = 11.84WRR62 pKa = 11.84RR63 pKa = 11.84SRR65 pKa = 11.84IPVIPAVLPQLDD77 pKa = 4.19PQDD80 pKa = 5.28LLDD83 pKa = 4.72LDD85 pKa = 4.42EE86 pKa = 5.48LLSEE90 pKa = 4.6ALPLVRR96 pKa = 11.84QRR98 pKa = 11.84HH99 pKa = 4.21SAIRR103 pKa = 11.84GLLTDD108 pKa = 3.55RR109 pKa = 11.84HH110 pKa = 6.26ALNKK114 pKa = 10.3LPEE117 pKa = 4.44GVFGIALGMPDD128 pKa = 4.34QNDD131 pKa = 3.21IEE133 pKa = 5.87DD134 pKa = 3.87NVFCGAVRR142 pKa = 11.84LGGFLVMNCHH152 pKa = 7.32AYY154 pKa = 9.14RR155 pKa = 11.84PKK157 pKa = 10.0MEE159 pKa = 4.08YY160 pKa = 8.36HH161 pKa = 5.35TTLVTKK167 pKa = 10.56RR168 pKa = 11.84GNFWMRR174 pKa = 11.84DD175 pKa = 3.44LVVTPDD181 pKa = 3.7PEE183 pKa = 4.28EE184 pKa = 4.23EE185 pKa = 4.15NVLEE189 pKa = 5.56DD190 pKa = 2.87MDD192 pKa = 5.1LFYY195 pKa = 11.21FRR197 pKa = 11.84LNPHH201 pKa = 6.42GLCSTKK207 pKa = 10.49AAEE210 pKa = 4.41TTVITQEE217 pKa = 4.24GVSARR222 pKa = 11.84IISLVNRR229 pKa = 11.84GVSSFGTVRR238 pKa = 11.84TFLEE242 pKa = 4.71GEE244 pKa = 4.24TTFGVVFYY252 pKa = 10.86SGKK255 pKa = 8.62TKK257 pKa = 10.21PGCSGFPYY265 pKa = 10.44LVGGKK270 pKa = 9.3VVGLHH275 pKa = 6.28CGDD278 pKa = 3.87YY279 pKa = 10.92NGTYY283 pKa = 10.74GDD285 pKa = 3.94GANVQLVLAKK295 pKa = 10.41LKK297 pKa = 10.6RR298 pKa = 11.84LSLGNRR304 pKa = 11.84HH305 pKa = 4.52SWFWVLDD312 pKa = 3.67DD313 pKa = 4.12RR314 pKa = 11.84HH315 pKa = 8.21AEE317 pKa = 3.93FTKK320 pKa = 9.99ISHH323 pKa = 6.35SGTPGEE329 pKa = 4.88LIVEE333 pKa = 4.15NSGRR337 pKa = 11.84YY338 pKa = 9.23YY339 pKa = 11.04YY340 pKa = 10.88VDD342 pKa = 3.22QEE344 pKa = 4.75DD345 pKa = 4.88YY346 pKa = 10.21LTEE349 pKa = 3.85ARR351 pKa = 11.84KK352 pKa = 10.16RR353 pKa = 11.84GVWAANHH360 pKa = 6.23KK361 pKa = 10.31GVFGRR366 pKa = 11.84FVGDD370 pKa = 3.37EE371 pKa = 4.09RR372 pKa = 11.84DD373 pKa = 3.58EE374 pKa = 3.98EE375 pKa = 5.14DD376 pKa = 3.64YY377 pKa = 11.69EE378 pKa = 4.13NDD380 pKa = 3.25RR381 pKa = 11.84RR382 pKa = 11.84SDD384 pKa = 3.51SDD386 pKa = 3.04HH387 pKa = 5.93GRR389 pKa = 11.84SRR391 pKa = 11.84SSARR395 pKa = 11.84SDD397 pKa = 3.87YY398 pKa = 11.46SDD400 pKa = 5.39DD401 pKa = 3.69DD402 pKa = 3.84HH403 pKa = 7.41FSNFEE408 pKa = 4.09DD409 pKa = 5.48DD410 pKa = 4.59EE411 pKa = 6.14FDD413 pKa = 4.05DD414 pKa = 4.34AHH416 pKa = 6.51SRR418 pKa = 11.84WVGRR422 pKa = 11.84QKK424 pKa = 10.85RR425 pKa = 11.84HH426 pKa = 5.13GKK428 pKa = 9.31SLSGFKK434 pKa = 10.06TGLNDD439 pKa = 4.31GNPDD443 pKa = 3.13SDD445 pKa = 3.82AVVSKK450 pKa = 10.78NVEE453 pKa = 4.0GPLPEE458 pKa = 4.87GPGYY462 pKa = 10.96AVTARR467 pKa = 11.84LRR469 pKa = 11.84FGADD473 pKa = 2.92PEE475 pKa = 3.95IRR477 pKa = 11.84MTSGEE482 pKa = 4.18IKK484 pKa = 10.52QEE486 pKa = 3.92AQEE489 pKa = 4.08IAACQKK495 pKa = 10.63KK496 pKa = 9.6IAEE499 pKa = 4.06LLARR503 pKa = 11.84VEE505 pKa = 4.14ALTTKK510 pKa = 10.45AQDD513 pKa = 3.29YY514 pKa = 9.9DD515 pKa = 3.62QLHH518 pKa = 5.36VQMEE522 pKa = 4.36KK523 pKa = 11.05DD524 pKa = 3.14RR525 pKa = 11.84VRR527 pKa = 11.84ALMEE531 pKa = 4.66AKK533 pKa = 10.24SQSDD537 pKa = 3.42QAAAQYY543 pKa = 9.11MVAISALKK551 pKa = 10.66SEE553 pKa = 4.26LAKK556 pKa = 10.65EE557 pKa = 3.82KK558 pKa = 10.86SEE560 pKa = 4.01RR561 pKa = 11.84LTTKK565 pKa = 10.77EE566 pKa = 4.46EE567 pKa = 3.88LHH569 pKa = 5.89QMHH572 pKa = 6.57LTVSEE577 pKa = 4.12LRR579 pKa = 11.84KK580 pKa = 10.01EE581 pKa = 4.05MRR583 pKa = 11.84SANARR588 pKa = 11.84LTEE591 pKa = 3.99AKK593 pKa = 10.52APVKK597 pKa = 10.22LKK599 pKa = 10.58PVVRR603 pKa = 11.84KK604 pKa = 6.53QTKK607 pKa = 8.46NEE609 pKa = 4.04KK610 pKa = 9.99KK611 pKa = 10.26VEE613 pKa = 4.04RR614 pKa = 11.84CLEE617 pKa = 4.37LGCMYY622 pKa = 10.33PKK624 pKa = 10.53EE625 pKa = 4.33ILLTMHH631 pKa = 7.01GPGLDD636 pKa = 3.45ALLQKK641 pKa = 10.89LEE643 pKa = 4.29ADD645 pKa = 4.38RR646 pKa = 11.84EE647 pKa = 4.23DD648 pKa = 3.91ASASRR653 pKa = 11.84QAGAIPRR660 pKa = 11.84NN661 pKa = 3.69

Molecular weight: 74.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEM1|A0A1L3KEM1_9VIRU RNA-directed RNA polymerase OS=Shahe sobemo-like virus 1 OX=1923454 PE=4 SV=1
MM1 pKa = 7.53FDD3 pKa = 3.01WPNRR7 pKa = 11.84NKK9 pKa = 10.73AAVDD13 pKa = 3.38EE14 pKa = 4.7SVRR17 pKa = 11.84QHH19 pKa = 5.43VKK21 pKa = 10.39HH22 pKa = 6.83RR23 pKa = 11.84DD24 pKa = 3.37DD25 pKa = 4.07VADD28 pKa = 3.49KK29 pKa = 9.98TVAPTRR35 pKa = 11.84EE36 pKa = 4.27EE37 pKa = 3.82YY38 pKa = 10.89SEE40 pKa = 4.01ILEE43 pKa = 4.26MTLKK47 pKa = 10.85AFGRR51 pKa = 11.84GWSLFNPDD59 pKa = 4.3EE60 pKa = 4.46ISVIRR65 pKa = 11.84IRR67 pKa = 11.84EE68 pKa = 4.19IIEE71 pKa = 3.94HH72 pKa = 5.58QVDD75 pKa = 3.92KK76 pKa = 10.94NSSPGYY82 pKa = 9.2PYY84 pKa = 10.75CLKK87 pKa = 10.21TKK89 pKa = 7.93TNKK92 pKa = 10.3DD93 pKa = 3.25FFGFDD98 pKa = 3.01ALGRR102 pKa = 11.84VPDD105 pKa = 4.28DD106 pKa = 3.79RR107 pKa = 11.84LAMVCGLVADD117 pKa = 6.05RR118 pKa = 11.84IDD120 pKa = 3.79EE121 pKa = 4.37LATRR125 pKa = 11.84PASDD129 pKa = 5.1PIRR132 pKa = 11.84VFIKK136 pKa = 10.46EE137 pKa = 4.03EE138 pKa = 3.54PASINKK144 pKa = 9.77RR145 pKa = 11.84STGALRR151 pKa = 11.84LISSVSVVDD160 pKa = 4.45TIVHH164 pKa = 6.29RR165 pKa = 11.84LFFGGVFDD173 pKa = 5.62KK174 pKa = 11.12LVKK177 pKa = 10.21HH178 pKa = 6.47WGATPNMAGWSLGGGKK194 pKa = 8.25WRR196 pKa = 11.84FFKK199 pKa = 10.75KK200 pKa = 9.87HH201 pKa = 5.36FKK203 pKa = 10.62GKK205 pKa = 10.77AFMADD210 pKa = 2.92KK211 pKa = 10.84KK212 pKa = 10.7KK213 pKa = 9.9WDD215 pKa = 3.42WSMQPWLANLTRR227 pKa = 11.84DD228 pKa = 3.25VLLNLGRR235 pKa = 11.84FEE237 pKa = 3.85GRR239 pKa = 11.84EE240 pKa = 3.6RR241 pKa = 11.84VIADD245 pKa = 3.2NALRR249 pKa = 11.84ALYY252 pKa = 10.42GMKK255 pKa = 10.28FEE257 pKa = 4.62GKK259 pKa = 9.76NYY261 pKa = 7.36PTTLVSACTRR271 pKa = 11.84CGACSFYY278 pKa = 10.3EE279 pKa = 3.86QRR281 pKa = 11.84TSGLQKK287 pKa = 10.63SGWLGTIITNGMQQYY302 pKa = 11.29ALAALAKK309 pKa = 9.43RR310 pKa = 11.84RR311 pKa = 11.84LGRR314 pKa = 11.84SMGPIFTMGDD324 pKa = 3.28DD325 pKa = 4.14TIEE328 pKa = 4.17EE329 pKa = 4.28DD330 pKa = 3.75VDD332 pKa = 4.42DD333 pKa = 4.86VEE335 pKa = 5.06APIYY339 pKa = 10.54VSEE342 pKa = 4.44LEE344 pKa = 4.12KK345 pKa = 10.95GGCIVGEE352 pKa = 4.18YY353 pKa = 10.45KK354 pKa = 9.69STEE357 pKa = 4.16DD358 pKa = 3.34YY359 pKa = 11.51DD360 pKa = 3.92FAGFTFPNGGGRR372 pKa = 11.84PVPNYY377 pKa = 10.01GDD379 pKa = 3.09KK380 pKa = 10.95HH381 pKa = 6.84AFTLAYY387 pKa = 10.23ANDD390 pKa = 3.97KK391 pKa = 10.82EE392 pKa = 5.17GVFADD397 pKa = 3.8EE398 pKa = 4.24QSRR401 pKa = 11.84VASYY405 pKa = 8.93ITMYY409 pKa = 11.15YY410 pKa = 9.72NDD412 pKa = 3.64KK413 pKa = 10.99ASYY416 pKa = 10.36RR417 pKa = 11.84KK418 pKa = 9.46LLQYY422 pKa = 11.12AAVLEE427 pKa = 4.3VDD429 pKa = 3.68QVPSDD434 pKa = 3.8FEE436 pKa = 4.29LMRR439 pKa = 11.84WYY441 pKa = 10.68GG442 pKa = 3.27

Molecular weight: 49.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1103

442

661

551.5

62.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.978 ± 0.103

1.269 ± 0.055

6.89 ± 0.356

6.8 ± 0.567

4.805 ± 0.387

8.069 ± 0.187

2.267 ± 0.422

3.989 ± 0.331

5.984 ± 0.497

8.794 ± 0.962

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.003

3.626 ± 0.004

3.898 ± 0.108

3.083 ± 0.507

6.981 ± 0.12

6.074 ± 0.259

4.624 ± 0.219

7.253 ± 0.288

1.451 ± 0.502

3.445 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski