Amino acid dipepetide frequency for Paenibacillus uliginis N3/975

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.169AlaAla: 7.169 ± 0.095	0.688AlaCys: 0.688 ± 0.019	3.743AlaAsp: 3.743 ± 0.055	5.031AlaGlu: 5.031 ± 0.068	3.103AlaPhe: 3.103 ± 0.047	5.913AlaGly: 5.913 ± 0.066	1.273AlaHis: 1.273 ± 0.029	5.063AlaIle: 5.063 ± 0.055	4.054AlaLys: 4.054 ± 0.059	7.531AlaLeu: 7.531 ± 0.074	2.133AlaMet: 2.133 ± 0.037	2.487AlaAsn: 2.487 ± 0.041	2.239AlaPro: 2.239 ± 0.039	2.401AlaGln: 2.401 ± 0.041	2.984AlaArg: 2.984 ± 0.045	4.621AlaSer: 4.621 ± 0.053	3.226AlaThr: 3.226 ± 0.051	6.044AlaVal: 6.044 ± 0.076	0.81AlaTrp: 0.81 ± 0.02	2.498AlaTyr: 2.498 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.504CysAla: 0.504 ± 0.018	0.108CysCys: 0.108 ± 0.008	0.438CysAsp: 0.438 ± 0.016	0.448CysGlu: 0.448 ± 0.017	0.356CysPhe: 0.356 ± 0.015	0.79CysGly: 0.79 ± 0.024	0.22CysHis: 0.22 ± 0.011	0.557CysIle: 0.557 ± 0.02	0.393CysLys: 0.393 ± 0.016	0.741CysLeu: 0.741 ± 0.019	0.237CysMet: 0.237 ± 0.012	0.308CysAsn: 0.308 ± 0.016	0.367CysPro: 0.367 ± 0.017	0.242CysGln: 0.242 ± 0.012	0.43CysArg: 0.43 ± 0.016	0.601CysSer: 0.601 ± 0.019	0.423CysThr: 0.423 ± 0.017	0.47CysVal: 0.47 ± 0.016	0.098CysTrp: 0.098 ± 0.006	0.293CysTyr: 0.293 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.411AspAla: 3.411 ± 0.053	0.422AspCys: 0.422 ± 0.014	2.573AspAsp: 2.573 ± 0.042	3.943AspGlu: 3.943 ± 0.051	2.226AspPhe: 2.226 ± 0.036	3.786AspGly: 3.786 ± 0.057	1.183AspHis: 1.183 ± 0.028	3.974AspIle: 3.974 ± 0.05	3.097AspLys: 3.097 ± 0.046	5.052AspLeu: 5.052 ± 0.059	1.548AspMet: 1.548 ± 0.03	2.047AspAsn: 2.047 ± 0.035	2.286AspPro: 2.286 ± 0.036	2.068AspGln: 2.068 ± 0.039	2.63AspArg: 2.63 ± 0.037	3.016AspSer: 3.016 ± 0.041	2.652AspThr: 2.652 ± 0.037	3.635AspVal: 3.635 ± 0.05	0.782AspTrp: 0.782 ± 0.022	2.135AspTyr: 2.135 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	5.443GluAla: 5.443 ± 0.063	0.45GluCys: 0.45 ± 0.017	3.504GluAsp: 3.504 ± 0.054	5.706GluGlu: 5.706 ± 0.071	2.311GluPhe: 2.311 ± 0.037	4.494GluGly: 4.494 ± 0.054	1.515GluHis: 1.515 ± 0.03	4.762GluIle: 4.762 ± 0.059	4.384GluLys: 4.384 ± 0.064	7.186GluLeu: 7.186 ± 0.073	2.193GluMet: 2.193 ± 0.043	2.805GluAsn: 2.805 ± 0.04	2.189GluPro: 2.189 ± 0.036	3.708GluGln: 3.708 ± 0.052	3.814GluArg: 3.814 ± 0.053	3.855GluSer: 3.855 ± 0.05	3.427GluThr: 3.427 ± 0.05	4.658GluVal: 4.658 ± 0.053	0.924GluTrp: 0.924 ± 0.024	2.231GluTyr: 2.231 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	2.962PheAla: 2.962 ± 0.043	0.4PheCys: 0.4 ± 0.014	2.297PheAsp: 2.297 ± 0.035	2.549PheGlu: 2.549 ± 0.039	1.936PhePhe: 1.936 ± 0.042	3.133PheGly: 3.133 ± 0.05	0.903PheHis: 0.903 ± 0.024	3.297PheIle: 3.297 ± 0.057	2.125PheLys: 2.125 ± 0.035	3.9PheLeu: 3.9 ± 0.061	1.321PheMet: 1.321 ± 0.028	1.798PheAsn: 1.798 ± 0.039	1.548PhePro: 1.548 ± 0.033	1.438PheGln: 1.438 ± 0.032	1.958PheArg: 1.958 ± 0.033	3.025PheSer: 3.025 ± 0.047	2.532PheThr: 2.532 ± 0.038	2.872PheVal: 2.872 ± 0.045	0.523PheTrp: 0.523 ± 0.021	1.557PheTyr: 1.557 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	5.034GlyAla: 5.034 ± 0.071	0.696GlyCys: 0.696 ± 0.021	3.456GlyAsp: 3.456 ± 0.045	4.461GlyGlu: 4.461 ± 0.058	3.268GlyPhe: 3.268 ± 0.046	5.208GlyGly: 5.208 ± 0.068	1.445GlyHis: 1.445 ± 0.031	5.841GlyIle: 5.841 ± 0.064	4.588GlyLys: 4.588 ± 0.054	7.021GlyLeu: 7.021 ± 0.067	2.43GlyMet: 2.43 ± 0.038	2.819GlyAsn: 2.819 ± 0.048	1.826GlyPro: 1.826 ± 0.037	2.574GlyGln: 2.574 ± 0.045	3.264GlyArg: 3.264 ± 0.047	4.699GlySer: 4.699 ± 0.063	4.266GlyThr: 4.266 ± 0.051	5.14GlyVal: 5.14 ± 0.055	0.974GlyTrp: 0.974 ± 0.028	2.995GlyTyr: 2.995 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.385HisAla: 1.385 ± 0.027	0.2HisCys: 0.2 ± 0.011	1.041HisAsp: 1.041 ± 0.023	1.394HisGlu: 1.394 ± 0.033	1.021HisPhe: 1.021 ± 0.024	1.481HisGly: 1.481 ± 0.033	0.606HisHis: 0.606 ± 0.019	1.529HisIle: 1.529 ± 0.029	0.969HisLys: 0.969 ± 0.027	2.083HisLeu: 2.083 ± 0.041	0.611HisMet: 0.611 ± 0.022	0.756HisAsn: 0.756 ± 0.021	1.125HisPro: 1.125 ± 0.027	0.815HisGln: 0.815 ± 0.021	1.081HisArg: 1.081 ± 0.027	1.257HisSer: 1.257 ± 0.028	1.103HisThr: 1.103 ± 0.025	1.424HisVal: 1.424 ± 0.034	0.281HisTrp: 0.281 ± 0.013	0.858HisTyr: 0.858 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.571IleAla: 5.571 ± 0.061	0.647IleCys: 0.647 ± 0.019	3.887IleAsp: 3.887 ± 0.047	4.708IleGlu: 4.708 ± 0.056	2.661IlePhe: 2.661 ± 0.048	5.474IleGly: 5.474 ± 0.064	1.661IleHis: 1.661 ± 0.031	4.956IleIle: 4.956 ± 0.076	3.547IleLys: 3.547 ± 0.05	6.416IleLeu: 6.416 ± 0.072	1.982IleMet: 1.982 ± 0.035	2.719IleAsn: 2.719 ± 0.044	3.325IlePro: 3.325 ± 0.044	2.797IleGln: 2.797 ± 0.04	3.594IleArg: 3.594 ± 0.047	5.157IleSer: 5.157 ± 0.061	4.157IleThr: 4.157 ± 0.059	5.235IleVal: 5.235 ± 0.061	0.739IleTrp: 0.739 ± 0.02	2.233IleTyr: 2.233 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.162LysAla: 4.162 ± 0.058	0.306LysCys: 0.306 ± 0.014	3.411LysAsp: 3.411 ± 0.045	4.92LysGlu: 4.92 ± 0.061	1.707LysPhe: 1.707 ± 0.034	3.847LysGly: 3.847 ± 0.054	1.233LysHis: 1.233 ± 0.028	3.6LysIle: 3.6 ± 0.052	3.895LysLys: 3.895 ± 0.057	5.628LysLeu: 5.628 ± 0.068	1.844LysMet: 1.844 ± 0.034	2.442LysAsn: 2.442 ± 0.038	2.343LysPro: 2.343 ± 0.035	2.67LysGln: 2.67 ± 0.038	2.9LysArg: 2.9 ± 0.043	3.435LysSer: 3.435 ± 0.052	3.008LysThr: 3.008 ± 0.04	3.954LysVal: 3.954 ± 0.054	0.729LysTrp: 0.729 ± 0.019	2.045LysTyr: 2.045 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	7.361LeuAla: 7.361 ± 0.078	0.846LeuCys: 0.846 ± 0.026	5.156LeuAsp: 5.156 ± 0.064	6.351LeuGlu: 6.351 ± 0.074	4.619LeuPhe: 4.619 ± 0.066	6.634LeuGly: 6.634 ± 0.065	2.016LeuHis: 2.016 ± 0.036	6.948LeuIle: 6.948 ± 0.088	5.892LeuLys: 5.892 ± 0.064	10.652LeuLeu: 10.652 ± 0.121	2.779LeuMet: 2.779 ± 0.045	4.29LeuAsn: 4.29 ± 0.052	4.241LeuPro: 4.241 ± 0.051	3.824LeuGln: 3.824 ± 0.048	4.576LeuArg: 4.576 ± 0.058	7.393LeuSer: 7.393 ± 0.071	5.618LeuThr: 5.618 ± 0.053	6.183LeuVal: 6.183 ± 0.066	1.024LeuTrp: 1.024 ± 0.027	3.362LeuTyr: 3.362 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	2.139MetAla: 2.139 ± 0.041	0.188MetCys: 0.188 ± 0.011	1.693MetAsp: 1.693 ± 0.03	2.028MetGlu: 2.028 ± 0.031	1.16MetPhe: 1.16 ± 0.027	1.897MetGly: 1.897 ± 0.04	0.469MetHis: 0.469 ± 0.016	2.202MetIle: 2.202 ± 0.036	2.29MetLys: 2.29 ± 0.036	3.08MetLeu: 3.08 ± 0.042	0.995MetMet: 0.995 ± 0.027	1.809MetAsn: 1.809 ± 0.033	1.121MetPro: 1.121 ± 0.029	1.011MetGln: 1.011 ± 0.024	1.235MetArg: 1.235 ± 0.029	1.985MetSer: 1.985 ± 0.029	1.752MetThr: 1.752 ± 0.03	1.898MetVal: 1.898 ± 0.032	0.261MetTrp: 0.261 ± 0.013	0.847MetTyr: 0.847 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.687AsnAla: 2.687 ± 0.041	0.292AsnCys: 0.292 ± 0.013	2.093AsnAsp: 2.093 ± 0.038	2.95AsnGlu: 2.95 ± 0.041	1.445AsnPhe: 1.445 ± 0.027	3.31AsnGly: 3.31 ± 0.049	0.974AsnHis: 0.974 ± 0.025	2.854AsnIle: 2.854 ± 0.044	2.393AsnLys: 2.393 ± 0.039	3.57AsnLeu: 3.57 ± 0.044	1.235AsnMet: 1.235 ± 0.027	1.867AsnAsn: 1.867 ± 0.033	2.087AsnPro: 2.087 ± 0.036	1.639AsnGln: 1.639 ± 0.029	2.204AsnArg: 2.204 ± 0.032	2.386AsnSer: 2.386 ± 0.035	2.147AsnThr: 2.147 ± 0.039	2.761AsnVal: 2.761 ± 0.046	0.533AsnTrp: 0.533 ± 0.016	1.493AsnTyr: 1.493 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	2.827ProAla: 2.827 ± 0.037	0.241ProCys: 0.241 ± 0.011	2.582ProAsp: 2.582 ± 0.04	3.401ProGlu: 3.401 ± 0.047	1.786ProPhe: 1.786 ± 0.035	2.767ProGly: 2.767 ± 0.053	0.822ProHis: 0.822 ± 0.022	2.518ProIle: 2.518 ± 0.04	1.94ProLys: 1.94 ± 0.034	3.805ProLeu: 3.805 ± 0.046	0.959ProMet: 0.959 ± 0.024	1.52ProAsn: 1.52 ± 0.031	1.183ProPro: 1.183 ± 0.031	1.324ProGln: 1.324 ± 0.029	1.34ProArg: 1.34 ± 0.026	2.559ProSer: 2.559 ± 0.045	1.923ProThr: 1.923 ± 0.037	3.132ProVal: 3.132 ± 0.042	0.487ProTrp: 0.487 ± 0.018	1.516ProTyr: 1.516 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	2.92GlnAla: 2.92 ± 0.046	0.233GlnCys: 0.233 ± 0.011	1.905GlnAsp: 1.905 ± 0.031	2.796GlnGlu: 2.796 ± 0.045	1.572GlnPhe: 1.572 ± 0.029	2.549GlnGly: 2.549 ± 0.038	0.827GlnHis: 0.827 ± 0.023	2.564GlnIle: 2.564 ± 0.04	2.239GlnLys: 2.239 ± 0.038	4.119GlnLeu: 4.119 ± 0.052	1.267GlnMet: 1.267 ± 0.026	1.507GlnAsn: 1.507 ± 0.035	1.402GlnPro: 1.402 ± 0.028	1.783GlnGln: 1.783 ± 0.04	1.797GlnArg: 1.797 ± 0.031	2.369GlnSer: 2.369 ± 0.032	1.864GlnThr: 1.864 ± 0.034	2.539GlnVal: 2.539 ± 0.042	0.507GlnTrp: 0.507 ± 0.017	1.423GlnTyr: 1.423 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.816ArgAla: 2.816 ± 0.044	0.379ArgCys: 0.379 ± 0.016	2.368ArgAsp: 2.368 ± 0.042	3.566ArgGlu: 3.566 ± 0.048	2.093ArgPhe: 2.093 ± 0.035	2.849ArgGly: 2.849 ± 0.045	1.038ArgHis: 1.038 ± 0.023	3.61ArgIle: 3.61 ± 0.049	3.23ArgLys: 3.23 ± 0.046	4.909ArgLeu: 4.909 ± 0.053	1.593ArgMet: 1.593 ± 0.03	2.063ArgAsn: 2.063 ± 0.032	1.58ArgPro: 1.58 ± 0.033	1.923ArgGln: 1.923 ± 0.04	2.542ArgArg: 2.542 ± 0.049	3.01ArgSer: 3.01 ± 0.037	2.484ArgThr: 2.484 ± 0.038	2.955ArgVal: 2.955 ± 0.039	0.601ArgTrp: 0.601 ± 0.018	1.824ArgTyr: 1.824 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	4.434SerAla: 4.434 ± 0.057	0.48SerCys: 0.48 ± 0.017	3.259SerAsp: 3.259 ± 0.048	4.121SerGlu: 4.121 ± 0.055	3.175SerPhe: 3.175 ± 0.047	5.44SerGly: 5.44 ± 0.061	1.308SerHis: 1.308 ± 0.027	4.788SerIle: 4.788 ± 0.056	3.622SerLys: 3.622 ± 0.049	6.651SerLeu: 6.651 ± 0.065	1.937SerMet: 1.937 ± 0.033	2.544SerAsn: 2.544 ± 0.043	2.505SerPro: 2.505 ± 0.04	2.147SerGln: 2.147 ± 0.038	3.147SerArg: 3.147 ± 0.045	4.688SerSer: 4.688 ± 0.057	3.371SerThr: 3.371 ± 0.047	4.681SerVal: 4.681 ± 0.05	0.845SerTrp: 0.845 ± 0.022	2.388SerTyr: 2.388 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.341ThrAla: 4.341 ± 0.051	0.394ThrCys: 0.394 ± 0.015	2.841ThrAsp: 2.841 ± 0.045	3.475ThrGlu: 3.475 ± 0.049	2.341ThrPhe: 2.341 ± 0.038	4.521ThrGly: 4.521 ± 0.083	1.035ThrHis: 1.035 ± 0.024	3.8ThrIle: 3.8 ± 0.049	2.652ThrLys: 2.652 ± 0.04	5.404ThrLeu: 5.404 ± 0.062	1.408ThrMet: 1.408 ± 0.029	1.989ThrAsn: 1.989 ± 0.034	2.464ThrPro: 2.464 ± 0.039	1.566ThrGln: 1.566 ± 0.033	2.105ThrArg: 2.105 ± 0.037	3.49ThrSer: 3.49 ± 0.046	2.843ThrThr: 2.843 ± 0.047	4.447ThrVal: 4.447 ± 0.049	0.603ThrTrp: 0.603 ± 0.016	1.889ThrTyr: 1.889 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	4.625ValAla: 4.625 ± 0.058	0.621ValCys: 0.621 ± 0.018	3.606ValAsp: 3.606 ± 0.048	4.455ValGlu: 4.455 ± 0.055	3.013ValPhe: 3.013 ± 0.051	4.438ValGly: 4.438 ± 0.055	1.45ValHis: 1.45 ± 0.032	5.312ValIle: 5.312 ± 0.063	4.137ValLys: 4.137 ± 0.049	7.231ValLeu: 7.231 ± 0.07	2.18ValMet: 2.18 ± 0.037	2.961ValAsn: 2.961 ± 0.038	2.902ValPro: 2.902 ± 0.045	2.56ValGln: 2.56 ± 0.041	3.218ValArg: 3.218 ± 0.05	4.893ValSer: 4.893 ± 0.053	4.304ValThr: 4.304 ± 0.056	4.897ValVal: 4.897 ± 0.061	0.835ValTrp: 0.835 ± 0.025	2.46ValTyr: 2.46 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.751TrpAla: 0.751 ± 0.022	0.094TrpCys: 0.094 ± 0.007	0.666TrpAsp: 0.666 ± 0.016	0.762TrpGlu: 0.762 ± 0.023	0.57TrpPhe: 0.57 ± 0.016	0.816TrpGly: 0.816 ± 0.023	0.245TrpHis: 0.245 ± 0.012	0.942TrpIle: 0.942 ± 0.023	0.776TrpLys: 0.776 ± 0.021	1.33TrpLeu: 1.33 ± 0.027	0.418TrpMet: 0.418 ± 0.014	0.698TrpAsn: 0.698 ± 0.021	0.334TrpPro: 0.334 ± 0.015	0.407TrpGln: 0.407 ± 0.015	0.568TrpArg: 0.568 ± 0.021	0.833TrpSer: 0.833 ± 0.023	0.61TrpThr: 0.61 ± 0.022	0.768TrpVal: 0.768 ± 0.023	0.184TrpTrp: 0.184 ± 0.011	0.408TrpTyr: 0.408 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.47TyrAla: 2.47 ± 0.04	0.337TyrCys: 0.337 ± 0.015	1.944TyrAsp: 1.944 ± 0.035	2.496TyrGlu: 2.496 ± 0.036	1.707TyrPhe: 1.707 ± 0.034	2.659TyrGly: 2.659 ± 0.04	0.799TyrHis: 0.799 ± 0.024	2.35TyrIle: 2.35 ± 0.041	1.856TyrLys: 1.856 ± 0.036	3.458TyrLeu: 3.458 ± 0.051	0.99TyrMet: 0.99 ± 0.027	1.494TyrAsn: 1.494 ± 0.031	1.563TyrPro: 1.563 ± 0.028	1.283TyrGln: 1.283 ± 0.027	2.047TyrArg: 2.047 ± 0.038	2.256TyrSer: 2.256 ± 0.038	1.9TyrThr: 1.9 ± 0.035	2.406TyrVal: 2.406 ± 0.04	0.44TyrTrp: 0.44 ± 0.019	1.435TyrTyr: 1.435 ± 0.035	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5718 proteins (1773498 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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