Pseudomicrostroma glucosiphilum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Exobasidiomycetes; Microstromatales; Microstromatales incertae sedis; Pseudomicrostroma

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A316U6N7|A0A316U6N7_9BASI Putative CCT6-component of chaperonin-containing T-complex OS=Pseudomicrostroma glucosiphilum OX=1684307 GN=BCV69DRAFT_283362 PE=3 SV=1
MM1 pKa = 7.64EE2 pKa = 5.3SMINKK7 pKa = 9.39AKK9 pKa = 10.47EE10 pKa = 3.79FASSEE15 pKa = 3.88KK16 pKa = 9.02GQNMMDD22 pKa = 3.14KK23 pKa = 11.0FGGNKK28 pKa = 9.7NNNNNNDD35 pKa = 3.51DD36 pKa = 3.59SSYY39 pKa = 11.61GGNNDD44 pKa = 2.98NSSYY48 pKa = 11.54GGGNDD53 pKa = 3.07NSSYY57 pKa = 11.09GGNDD61 pKa = 3.03NSSGGGGGGFGMQDD75 pKa = 2.98ASSGGDD81 pKa = 3.11SYY83 pKa = 12.02GSGGGQGGFGGDD95 pKa = 3.12TSGQGGYY102 pKa = 9.52GANDD106 pKa = 3.74GGDD109 pKa = 3.91SYY111 pKa = 11.85SSSNQQGGGGYY122 pKa = 9.62GGSGGGQFGDD132 pKa = 4.31DD133 pKa = 3.56SSSGGGRR140 pKa = 11.84QGGGGFGGGGQDD152 pKa = 3.97DD153 pKa = 4.69FSSGGARR160 pKa = 11.84QGGGGGFGGGGQDD173 pKa = 4.18DD174 pKa = 4.49FSSGGGQGGQGGGFGGDD191 pKa = 3.04TSGQGGYY198 pKa = 9.52GANDD202 pKa = 3.79GGDD205 pKa = 3.68SFSSSNQQGGGYY217 pKa = 9.19GGSGGGQFGDD227 pKa = 4.15DD228 pKa = 3.83SGSGGGGGGYY238 pKa = 10.48GGGGRR243 pKa = 11.84DD244 pKa = 3.43DD245 pKa = 3.93QYY247 pKa = 12.23

Molecular weight: 22.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A316U8R9|A0A316U8R9_9BASI Threonine synthase OS=Pseudomicrostroma glucosiphilum OX=1684307 GN=BCV69DRAFT_282335 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 5.0GALAAAIKK10 pKa = 9.89QGKK13 pKa = 8.37ALKK16 pKa = 10.02KK17 pKa = 10.24AQTNDD22 pKa = 2.75RR23 pKa = 11.84SAPAIAGSVGGSGGGGRR40 pKa = 11.84PAGSAPPVPGAAAASAMASAAVTKK64 pKa = 7.32MTSSGPPNLGGLFAGGMPTLRR85 pKa = 11.84SASGGSTAPKK95 pKa = 9.84PPGRR99 pKa = 11.84PAPPPASSAPAQPPPARR116 pKa = 11.84GVPTPPRR123 pKa = 11.84ASAPPPPAPPNRR135 pKa = 11.84PTAAAPPPPPGRR147 pKa = 11.84PAPPASGPPPPPPPPPGRR165 pKa = 11.84PASSAPPPPTRR176 pKa = 11.84PTSSAPPPPPPPPAAPPTPSRR197 pKa = 11.84STLAPAAPPPPPPPPGPSKK216 pKa = 10.64GAPPPPSRR224 pKa = 11.84PSAVPPRR231 pKa = 11.84APPPPQPQTSAPSTPSRR248 pKa = 11.84SVPAPPSRR256 pKa = 11.84PAPGASAAPPPAPPPPPPPSAAPSTPSRR284 pKa = 11.84SVPTAPSRR292 pKa = 11.84LAPSGASAAPPPPAPSRR309 pKa = 11.84LAPSGASAAPPPPAPPPPPPASRR332 pKa = 11.84APAPPPPSRR341 pKa = 11.84APAPPPPSRR350 pKa = 11.84STPTSRR356 pKa = 11.84PPPPPVAASAATNGSGRR373 pKa = 11.84STPITNGANGAAPPLRR389 pKa = 11.84NSIVGPPSNGYY400 pKa = 9.03GAGGGGGGGRR410 pKa = 11.84SKK412 pKa = 11.34

Molecular weight: 38.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6679

0

6679

3653858

50

9118

547.1

59.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.411 ± 0.095

0.992 ± 0.011

5.343 ± 0.021

6.259 ± 0.031

3.033 ± 0.017

7.855 ± 0.031

2.241 ± 0.014

3.804 ± 0.02

4.431 ± 0.027

8.928 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.866 ± 0.01

2.805 ± 0.013

6.227 ± 0.032

4.114 ± 0.023

6.587 ± 0.03

10.199 ± 0.075

5.775 ± 0.026

5.693 ± 0.02

1.238 ± 0.009

2.196 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski