Amino acid dipepetide frequency for Microbacterium phage Knox

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.422AlaAla: 11.422 ± 1.037
0.605AlaCys: 0.605 ± 0.247
4.766AlaAsp: 4.766 ± 0.666
5.976AlaGlu: 5.976 ± 0.671
2.874AlaPhe: 2.874 ± 0.439
7.867AlaGly: 7.867 ± 1.224
1.967AlaHis: 1.967 ± 0.352
5.295AlaIle: 5.295 ± 0.993
5.144AlaLys: 5.144 ± 0.629
8.548AlaLeu: 8.548 ± 0.996
2.572AlaMet: 2.572 ± 0.468
3.026AlaAsn: 3.026 ± 0.433
4.236AlaPro: 4.236 ± 0.689
4.312AlaGln: 4.312 ± 0.595
5.825AlaArg: 5.825 ± 0.935
5.371AlaSer: 5.371 ± 0.618
6.203AlaThr: 6.203 ± 0.683
6.657AlaVal: 6.657 ± 0.642
1.967AlaTrp: 1.967 ± 0.428
2.723AlaTyr: 2.723 ± 0.517
0.0AlaXaa: 0.0 ± 0.0
Cys
0.378CysAla: 0.378 ± 0.156
0.0CysCys: 0.0 ± 0.0
0.303CysAsp: 0.303 ± 0.141
0.151CysGlu: 0.151 ± 0.112
0.151CysPhe: 0.151 ± 0.1
0.832CysGly: 0.832 ± 0.27
0.227CysHis: 0.227 ± 0.114
0.0CysIle: 0.0 ± 0.0
0.53CysLys: 0.53 ± 0.197
0.605CysLeu: 0.605 ± 0.245
0.076CysMet: 0.076 ± 0.084
0.227CysAsn: 0.227 ± 0.128
0.681CysPro: 0.681 ± 0.244
0.076CysGln: 0.076 ± 0.071
0.303CysArg: 0.303 ± 0.149
0.454CysSer: 0.454 ± 0.178
0.378CysThr: 0.378 ± 0.178
0.53CysVal: 0.53 ± 0.201
0.227CysTrp: 0.227 ± 0.12
0.454CysTyr: 0.454 ± 0.206
0.0CysXaa: 0.0 ± 0.0
Asp
4.992AspAla: 4.992 ± 0.581
0.756AspCys: 0.756 ± 0.212
5.371AspAsp: 5.371 ± 0.95
5.825AspGlu: 5.825 ± 1.221
2.194AspPhe: 2.194 ± 0.377
4.463AspGly: 4.463 ± 0.664
1.135AspHis: 1.135 ± 0.223
3.253AspIle: 3.253 ± 0.376
2.648AspLys: 2.648 ± 0.446
5.295AspLeu: 5.295 ± 0.711
1.362AspMet: 1.362 ± 0.298
1.513AspAsn: 1.513 ± 0.391
4.387AspPro: 4.387 ± 0.61
1.967AspGln: 1.967 ± 0.464
3.328AspArg: 3.328 ± 0.523
3.404AspSer: 3.404 ± 0.494
2.723AspThr: 2.723 ± 0.45
4.766AspVal: 4.766 ± 0.631
2.194AspTrp: 2.194 ± 0.371
2.723AspTyr: 2.723 ± 0.449
0.0AspXaa: 0.0 ± 0.0
Glu
7.867GluAla: 7.867 ± 0.743
0.303GluCys: 0.303 ± 0.197
4.917GluAsp: 4.917 ± 1.026
5.825GluGlu: 5.825 ± 1.274
2.042GluPhe: 2.042 ± 0.452
4.312GluGly: 4.312 ± 0.681
0.983GluHis: 0.983 ± 0.277
3.026GluIle: 3.026 ± 0.592
2.194GluLys: 2.194 ± 0.45
5.522GluLeu: 5.522 ± 0.707
2.421GluMet: 2.421 ± 0.475
1.967GluAsn: 1.967 ± 0.411
2.799GluPro: 2.799 ± 0.545
2.572GluGln: 2.572 ± 0.583
3.631GluArg: 3.631 ± 0.541
2.723GluSer: 2.723 ± 0.573
3.328GluThr: 3.328 ± 0.508
5.295GluVal: 5.295 ± 0.513
1.513GluTrp: 1.513 ± 0.302
1.967GluTyr: 1.967 ± 0.296
0.0GluXaa: 0.0 ± 0.0
Phe
2.648PheAla: 2.648 ± 0.447
0.151PheCys: 0.151 ± 0.103
2.496PheAsp: 2.496 ± 0.435
1.967PheGlu: 1.967 ± 0.387
0.605PhePhe: 0.605 ± 0.208
3.404PheGly: 3.404 ± 0.502
0.756PheHis: 0.756 ± 0.229
1.059PheIle: 1.059 ± 0.266
1.74PheLys: 1.74 ± 0.332
2.042PheLeu: 2.042 ± 0.439
0.53PheMet: 0.53 ± 0.293
0.983PheAsn: 0.983 ± 0.226
1.21PhePro: 1.21 ± 0.284
1.21PheGln: 1.21 ± 0.314
2.572PheArg: 2.572 ± 0.428
1.589PheSer: 1.589 ± 0.299
2.496PheThr: 2.496 ± 0.477
1.74PheVal: 1.74 ± 0.405
0.681PheTrp: 0.681 ± 0.305
0.756PheTyr: 0.756 ± 0.299
0.0PheXaa: 0.0 ± 0.0
Gly
6.43GlyAla: 6.43 ± 0.963
0.756GlyCys: 0.756 ± 0.26
3.933GlyAsp: 3.933 ± 0.509
3.933GlyGlu: 3.933 ± 0.53
2.95GlyPhe: 2.95 ± 0.365
5.068GlyGly: 5.068 ± 0.876
1.891GlyHis: 1.891 ± 0.339
5.144GlyIle: 5.144 ± 0.922
5.068GlyLys: 5.068 ± 0.918
6.127GlyLeu: 6.127 ± 0.783
2.421GlyMet: 2.421 ± 0.418
2.269GlyAsn: 2.269 ± 0.412
3.555GlyPro: 3.555 ± 0.641
3.404GlyGln: 3.404 ± 0.645
4.539GlyArg: 4.539 ± 0.651
5.295GlySer: 5.295 ± 0.68
6.051GlyThr: 6.051 ± 0.729
6.278GlyVal: 6.278 ± 0.716
1.513GlyTrp: 1.513 ± 0.28
2.118GlyTyr: 2.118 ± 0.543
0.0GlyXaa: 0.0 ± 0.0
His
1.059HisAla: 1.059 ± 0.265
0.151HisCys: 0.151 ± 0.123
0.983HisAsp: 0.983 ± 0.257
1.286HisGlu: 1.286 ± 0.28
0.908HisPhe: 0.908 ± 0.267
1.664HisGly: 1.664 ± 0.315
0.378HisHis: 0.378 ± 0.178
0.756HisIle: 0.756 ± 0.265
1.21HisLys: 1.21 ± 0.426
1.21HisLeu: 1.21 ± 0.234
0.378HisMet: 0.378 ± 0.18
0.832HisAsn: 0.832 ± 0.276
0.605HisPro: 0.605 ± 0.229
0.454HisGln: 0.454 ± 0.175
0.832HisArg: 0.832 ± 0.254
1.664HisSer: 1.664 ± 0.316
1.362HisThr: 1.362 ± 0.3
1.74HisVal: 1.74 ± 0.359
0.454HisTrp: 0.454 ± 0.129
0.832HisTyr: 0.832 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
5.446IleAla: 5.446 ± 0.584
0.303IleCys: 0.303 ± 0.143
4.766IleAsp: 4.766 ± 0.516
3.101IleGlu: 3.101 ± 0.625
0.681IlePhe: 0.681 ± 0.296
4.16IleGly: 4.16 ± 0.782
0.832IleHis: 0.832 ± 0.239
3.026IleIle: 3.026 ± 0.87
2.496IleLys: 2.496 ± 0.478
2.95IleLeu: 2.95 ± 0.485
1.362IleMet: 1.362 ± 0.342
1.74IleAsn: 1.74 ± 0.38
2.799IlePro: 2.799 ± 0.513
2.723IleGln: 2.723 ± 0.575
2.572IleArg: 2.572 ± 0.469
3.328IleSer: 3.328 ± 0.503
3.253IleThr: 3.253 ± 0.763
2.723IleVal: 2.723 ± 0.495
0.756IleTrp: 0.756 ± 0.255
1.589IleTyr: 1.589 ± 0.364
0.0IleXaa: 0.0 ± 0.0
Lys
4.69LysAla: 4.69 ± 0.69
0.303LysCys: 0.303 ± 0.136
2.345LysAsp: 2.345 ± 0.453
3.404LysGlu: 3.404 ± 0.569
1.059LysPhe: 1.059 ± 0.261
3.404LysGly: 3.404 ± 0.538
0.983LysHis: 0.983 ± 0.288
2.042LysIle: 2.042 ± 0.425
2.345LysLys: 2.345 ± 0.589
4.387LysLeu: 4.387 ± 0.588
1.362LysMet: 1.362 ± 0.36
1.513LysAsn: 1.513 ± 0.381
3.404LysPro: 3.404 ± 0.61
2.042LysGln: 2.042 ± 0.524
2.799LysArg: 2.799 ± 0.491
2.269LysSer: 2.269 ± 0.419
2.496LysThr: 2.496 ± 0.338
3.631LysVal: 3.631 ± 0.521
1.059LysTrp: 1.059 ± 0.249
1.362LysTyr: 1.362 ± 0.276
0.0LysXaa: 0.0 ± 0.0
Leu
8.472LeuAla: 8.472 ± 0.663
0.605LeuCys: 0.605 ± 0.178
6.278LeuAsp: 6.278 ± 0.617
6.051LeuGlu: 6.051 ± 0.804
2.042LeuPhe: 2.042 ± 0.316
6.203LeuGly: 6.203 ± 0.716
1.21LeuHis: 1.21 ± 0.292
4.992LeuIle: 4.992 ± 1.051
4.69LeuLys: 4.69 ± 0.611
7.867LeuLeu: 7.867 ± 0.729
2.118LeuMet: 2.118 ± 0.384
2.874LeuAsn: 2.874 ± 0.416
4.085LeuPro: 4.085 ± 0.58
2.723LeuGln: 2.723 ± 0.481
5.673LeuArg: 5.673 ± 0.592
4.312LeuSer: 4.312 ± 0.546
5.598LeuThr: 5.598 ± 0.619
6.354LeuVal: 6.354 ± 0.674
1.286LeuTrp: 1.286 ± 0.285
1.891LeuTyr: 1.891 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
2.95MetAla: 2.95 ± 0.45
0.227MetCys: 0.227 ± 0.125
1.664MetAsp: 1.664 ± 0.352
1.059MetGlu: 1.059 ± 0.257
0.681MetPhe: 0.681 ± 0.194
1.815MetGly: 1.815 ± 0.454
0.151MetHis: 0.151 ± 0.108
1.21MetIle: 1.21 ± 0.279
0.378MetLys: 0.378 ± 0.17
2.648MetLeu: 2.648 ± 0.509
0.605MetMet: 0.605 ± 0.173
0.983MetAsn: 0.983 ± 0.226
1.74MetPro: 1.74 ± 0.328
0.983MetGln: 0.983 ± 0.292
0.605MetArg: 0.605 ± 0.196
3.177MetSer: 3.177 ± 0.47
2.042MetThr: 2.042 ± 0.38
1.74MetVal: 1.74 ± 0.298
0.151MetTrp: 0.151 ± 0.099
0.378MetTyr: 0.378 ± 0.149
0.0MetXaa: 0.0 ± 0.0
Asn
2.874AsnAla: 2.874 ± 0.511
0.076AsnCys: 0.076 ± 0.074
1.891AsnAsp: 1.891 ± 0.361
1.891AsnGlu: 1.891 ± 0.431
0.756AsnPhe: 0.756 ± 0.241
3.101AsnGly: 3.101 ± 0.439
0.605AsnHis: 0.605 ± 0.2
1.815AsnIle: 1.815 ± 0.432
1.589AsnLys: 1.589 ± 0.283
3.253AsnLeu: 3.253 ± 0.433
0.378AsnMet: 0.378 ± 0.172
1.135AsnAsn: 1.135 ± 0.309
1.891AsnPro: 1.891 ± 0.403
1.437AsnGln: 1.437 ± 0.338
1.815AsnArg: 1.815 ± 0.412
2.194AsnSer: 2.194 ± 0.456
1.815AsnThr: 1.815 ± 0.403
1.664AsnVal: 1.664 ± 0.361
0.756AsnTrp: 0.756 ± 0.244
1.135AsnTyr: 1.135 ± 0.301
0.0AsnXaa: 0.0 ± 0.0
Pro
5.9ProAla: 5.9 ± 0.777
0.0ProCys: 0.0 ± 0.0
3.253ProAsp: 3.253 ± 0.531
3.782ProGlu: 3.782 ± 0.679
1.967ProPhe: 1.967 ± 0.471
4.009ProGly: 4.009 ± 0.556
0.681ProHis: 0.681 ± 0.264
2.194ProIle: 2.194 ± 0.313
1.664ProLys: 1.664 ± 0.336
3.782ProLeu: 3.782 ± 0.504
1.059ProMet: 1.059 ± 0.288
1.74ProAsn: 1.74 ± 0.424
1.286ProPro: 1.286 ± 0.403
2.648ProGln: 2.648 ± 0.44
2.042ProArg: 2.042 ± 0.504
2.874ProSer: 2.874 ± 0.405
3.858ProThr: 3.858 ± 0.689
4.539ProVal: 4.539 ± 0.548
0.681ProTrp: 0.681 ± 0.212
1.21ProTyr: 1.21 ± 0.255
0.0ProXaa: 0.0 ± 0.0
Gln
4.009GlnAla: 4.009 ± 0.627
0.227GlnCys: 0.227 ± 0.138
1.815GlnAsp: 1.815 ± 0.359
3.177GlnGlu: 3.177 ± 0.675
0.681GlnPhe: 0.681 ± 0.229
3.48GlnGly: 3.48 ± 0.519
1.437GlnHis: 1.437 ± 0.371
1.589GlnIle: 1.589 ± 0.384
1.059GlnLys: 1.059 ± 0.238
4.085GlnLeu: 4.085 ± 0.668
0.908GlnMet: 0.908 ± 0.276
1.664GlnAsn: 1.664 ± 0.351
1.815GlnPro: 1.815 ± 0.427
2.496GlnGln: 2.496 ± 0.758
2.874GlnArg: 2.874 ± 0.458
2.194GlnSer: 2.194 ± 0.439
2.648GlnThr: 2.648 ± 0.375
2.269GlnVal: 2.269 ± 0.386
1.286GlnTrp: 1.286 ± 0.329
0.983GlnTyr: 0.983 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
4.766ArgAla: 4.766 ± 0.608
0.378ArgCys: 0.378 ± 0.158
3.858ArgAsp: 3.858 ± 0.407
3.253ArgGlu: 3.253 ± 0.565
2.269ArgPhe: 2.269 ± 0.462
3.328ArgGly: 3.328 ± 0.452
0.983ArgHis: 0.983 ± 0.264
2.799ArgIle: 2.799 ± 0.377
3.101ArgLys: 3.101 ± 0.576
5.295ArgLeu: 5.295 ± 0.7
1.967ArgMet: 1.967 ± 0.413
1.74ArgAsn: 1.74 ± 0.36
2.723ArgPro: 2.723 ± 0.479
2.345ArgGln: 2.345 ± 0.351
4.16ArgArg: 4.16 ± 0.624
4.236ArgSer: 4.236 ± 0.597
3.253ArgThr: 3.253 ± 0.591
4.463ArgVal: 4.463 ± 0.61
0.832ArgTrp: 0.832 ± 0.218
1.664ArgTyr: 1.664 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
6.127SerAla: 6.127 ± 0.8
0.303SerCys: 0.303 ± 0.18
3.555SerAsp: 3.555 ± 0.505
3.026SerGlu: 3.026 ± 0.54
2.648SerPhe: 2.648 ± 0.446
5.144SerGly: 5.144 ± 0.794
1.059SerHis: 1.059 ± 0.296
2.799SerIle: 2.799 ± 0.585
2.874SerLys: 2.874 ± 0.448
5.295SerLeu: 5.295 ± 0.51
2.042SerMet: 2.042 ± 0.415
1.74SerAsn: 1.74 ± 0.394
2.572SerPro: 2.572 ± 0.301
2.723SerGln: 2.723 ± 0.449
3.101SerArg: 3.101 ± 0.477
3.782SerSer: 3.782 ± 0.564
3.555SerThr: 3.555 ± 0.579
4.539SerVal: 4.539 ± 0.527
1.362SerTrp: 1.362 ± 0.361
1.967SerTyr: 1.967 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
5.522ThrAla: 5.522 ± 0.972
0.303ThrCys: 0.303 ± 0.151
3.253ThrAsp: 3.253 ± 0.391
3.933ThrGlu: 3.933 ± 0.562
2.799ThrPhe: 2.799 ± 0.418
5.673ThrGly: 5.673 ± 0.648
0.983ThrHis: 0.983 ± 0.352
3.328ThrIle: 3.328 ± 0.521
2.345ThrLys: 2.345 ± 0.472
6.278ThrLeu: 6.278 ± 0.691
1.286ThrMet: 1.286 ± 0.345
1.21ThrAsn: 1.21 ± 0.329
3.328ThrPro: 3.328 ± 0.473
2.269ThrGln: 2.269 ± 0.334
3.48ThrArg: 3.48 ± 0.522
3.631ThrSer: 3.631 ± 0.608
5.068ThrThr: 5.068 ± 0.746
5.976ThrVal: 5.976 ± 0.664
1.286ThrTrp: 1.286 ± 0.289
2.194ThrTyr: 2.194 ± 0.417
0.0ThrXaa: 0.0 ± 0.0
Val
7.943ValAla: 7.943 ± 0.749
0.605ValCys: 0.605 ± 0.231
4.766ValAsp: 4.766 ± 0.638
4.463ValGlu: 4.463 ± 0.518
1.74ValPhe: 1.74 ± 0.357
5.673ValGly: 5.673 ± 0.711
1.513ValHis: 1.513 ± 0.348
3.782ValIle: 3.782 ± 0.592
3.631ValLys: 3.631 ± 0.535
5.825ValLeu: 5.825 ± 0.7
1.664ValMet: 1.664 ± 0.419
3.328ValAsn: 3.328 ± 0.444
3.555ValPro: 3.555 ± 0.519
2.874ValGln: 2.874 ± 0.391
4.085ValArg: 4.085 ± 0.627
4.312ValSer: 4.312 ± 0.604
5.068ValThr: 5.068 ± 0.599
5.295ValVal: 5.295 ± 0.685
1.664ValTrp: 1.664 ± 0.415
2.648ValTyr: 2.648 ± 0.426
0.0ValXaa: 0.0 ± 0.0
Trp
1.286TrpAla: 1.286 ± 0.351
0.227TrpCys: 0.227 ± 0.127
1.891TrpAsp: 1.891 ± 0.337
1.21TrpGlu: 1.21 ± 0.231
0.756TrpPhe: 0.756 ± 0.23
1.74TrpGly: 1.74 ± 0.392
0.53TrpHis: 0.53 ± 0.165
1.135TrpIle: 1.135 ± 0.287
0.756TrpLys: 0.756 ± 0.239
2.118TrpLeu: 2.118 ± 0.432
0.227TrpMet: 0.227 ± 0.119
0.605TrpAsn: 0.605 ± 0.176
1.059TrpPro: 1.059 ± 0.285
0.681TrpGln: 0.681 ± 0.292
1.059TrpArg: 1.059 ± 0.265
0.983TrpSer: 0.983 ± 0.306
1.589TrpThr: 1.589 ± 0.256
1.664TrpVal: 1.664 ± 0.367
0.681TrpTrp: 0.681 ± 0.28
1.135TrpTyr: 1.135 ± 0.282
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.572TyrAla: 2.572 ± 0.368
0.303TyrCys: 0.303 ± 0.151
2.194TyrAsp: 2.194 ± 0.328
1.891TyrGlu: 1.891 ± 0.346
0.832TyrPhe: 0.832 ± 0.208
3.404TyrGly: 3.404 ± 0.553
0.454TyrHis: 0.454 ± 0.189
1.362TyrIle: 1.362 ± 0.281
1.437TyrLys: 1.437 ± 0.337
2.194TyrLeu: 2.194 ± 0.328
0.378TyrMet: 0.378 ± 0.203
0.983TyrAsn: 0.983 ± 0.332
1.513TyrPro: 1.513 ± 0.379
0.756TyrGln: 0.756 ± 0.244
2.118TyrArg: 2.118 ± 0.418
2.421TyrSer: 2.421 ± 0.393
1.362TyrThr: 1.362 ± 0.224
2.572TyrVal: 2.572 ± 0.55
0.983TyrTrp: 0.983 ± 0.26
1.21TyrTyr: 1.21 ± 0.361
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13221 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski