Amino acid dipepetide frequency for Mycobacterium phage Timshel

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.435AlaAla: 11.435 ± 1.207
0.898AlaCys: 0.898 ± 0.211
6.586AlaAsp: 6.586 ± 0.548
5.388AlaGlu: 5.388 ± 0.578
3.951AlaPhe: 3.951 ± 0.526
8.442AlaGly: 8.442 ± 0.941
2.036AlaHis: 2.036 ± 0.351
4.55AlaIle: 4.55 ± 0.514
4.011AlaLys: 4.011 ± 0.565
7.424AlaLeu: 7.424 ± 0.641
2.814AlaMet: 2.814 ± 0.398
2.634AlaAsn: 2.634 ± 0.481
4.849AlaPro: 4.849 ± 0.55
4.49AlaGln: 4.49 ± 0.599
5.149AlaArg: 5.149 ± 0.698
5.328AlaSer: 5.328 ± 0.587
5.328AlaThr: 5.328 ± 0.474
8.322AlaVal: 8.322 ± 0.704
2.215AlaTrp: 2.215 ± 0.379
2.754AlaTyr: 2.754 ± 0.509
0.0AlaXaa: 0.0 ± 0.0
Cys
0.599CysAla: 0.599 ± 0.197
0.12CysCys: 0.12 ± 0.132
0.479CysAsp: 0.479 ± 0.166
0.718CysGlu: 0.718 ± 0.211
0.18CysPhe: 0.18 ± 0.092
0.898CysGly: 0.898 ± 0.278
0.18CysHis: 0.18 ± 0.098
0.359CysIle: 0.359 ± 0.137
0.359CysLys: 0.359 ± 0.125
0.778CysLeu: 0.778 ± 0.224
0.18CysMet: 0.18 ± 0.1
0.419CysAsn: 0.419 ± 0.162
0.359CysPro: 0.359 ± 0.135
0.239CysGln: 0.239 ± 0.137
0.599CysArg: 0.599 ± 0.183
0.419CysSer: 0.419 ± 0.15
0.299CysThr: 0.299 ± 0.166
0.599CysVal: 0.599 ± 0.212
0.359CysTrp: 0.359 ± 0.162
0.18CysTyr: 0.18 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
6.705AspAla: 6.705 ± 0.574
0.419AspCys: 0.419 ± 0.187
3.892AspAsp: 3.892 ± 0.574
5.089AspGlu: 5.089 ± 0.604
1.856AspPhe: 1.856 ± 0.376
5.987AspGly: 5.987 ± 0.611
1.437AspHis: 1.437 ± 0.327
2.335AspIle: 2.335 ± 0.434
2.574AspLys: 2.574 ± 0.474
5.628AspLeu: 5.628 ± 0.505
1.317AspMet: 1.317 ± 0.297
1.616AspAsn: 1.616 ± 0.302
4.79AspPro: 4.79 ± 0.594
1.796AspGln: 1.796 ± 0.344
3.712AspArg: 3.712 ± 0.448
3.113AspSer: 3.113 ± 0.39
3.592AspThr: 3.592 ± 0.417
4.849AspVal: 4.849 ± 0.464
1.616AspTrp: 1.616 ± 0.332
2.036AspTyr: 2.036 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
6.526GluAla: 6.526 ± 0.692
0.479GluCys: 0.479 ± 0.209
4.49GluAsp: 4.49 ± 0.509
3.413GluGlu: 3.413 ± 0.44
2.275GluPhe: 2.275 ± 0.291
5.269GluGly: 5.269 ± 0.641
1.377GluHis: 1.377 ± 0.313
3.293GluIle: 3.293 ± 0.388
2.754GluLys: 2.754 ± 0.362
5.149GluLeu: 5.149 ± 0.566
1.497GluMet: 1.497 ± 0.369
1.078GluAsn: 1.078 ± 0.218
2.634GluPro: 2.634 ± 0.383
2.574GluGln: 2.574 ± 0.427
3.951GluArg: 3.951 ± 0.559
3.113GluSer: 3.113 ± 0.364
2.814GluThr: 2.814 ± 0.405
5.508GluVal: 5.508 ± 0.468
1.257GluTrp: 1.257 ± 0.295
1.796GluTyr: 1.796 ± 0.438
0.0GluXaa: 0.0 ± 0.0
Phe
2.754PheAla: 2.754 ± 0.421
0.239PheCys: 0.239 ± 0.096
2.515PheAsp: 2.515 ± 0.382
2.275PheGlu: 2.275 ± 0.371
0.539PhePhe: 0.539 ± 0.175
3.592PheGly: 3.592 ± 0.452
0.419PheHis: 0.419 ± 0.18
1.676PheIle: 1.676 ± 0.336
1.138PheLys: 1.138 ± 0.251
2.754PheLeu: 2.754 ± 0.354
0.599PheMet: 0.599 ± 0.211
1.138PheAsn: 1.138 ± 0.258
1.916PhePro: 1.916 ± 0.363
1.497PheGln: 1.497 ± 0.325
1.856PheArg: 1.856 ± 0.287
1.976PheSer: 1.976 ± 0.285
2.574PheThr: 2.574 ± 0.354
2.515PheVal: 2.515 ± 0.435
0.599PheTrp: 0.599 ± 0.222
0.778PheTyr: 0.778 ± 0.21
0.0PheXaa: 0.0 ± 0.0
Gly
7.783GlyAla: 7.783 ± 1.051
1.078GlyCys: 1.078 ± 0.254
6.107GlyAsp: 6.107 ± 0.933
5.628GlyGlu: 5.628 ± 0.529
2.814GlyPhe: 2.814 ± 0.376
11.495GlyGly: 11.495 ± 2.768
2.275GlyHis: 2.275 ± 0.457
5.269GlyIle: 5.269 ± 0.574
4.67GlyLys: 4.67 ± 0.608
7.244GlyLeu: 7.244 ± 0.835
2.275GlyMet: 2.275 ± 0.307
3.353GlyAsn: 3.353 ± 0.6
6.586GlyPro: 6.586 ± 2.222
2.874GlyGln: 2.874 ± 0.353
4.311GlyArg: 4.311 ± 0.649
4.55GlySer: 4.55 ± 0.6
6.107GlyThr: 6.107 ± 0.647
6.825GlyVal: 6.825 ± 0.649
2.275GlyTrp: 2.275 ± 0.337
2.634GlyTyr: 2.634 ± 0.445
0.0GlyXaa: 0.0 ± 0.0
His
1.616HisAla: 1.616 ± 0.313
0.06HisCys: 0.06 ± 0.056
1.676HisAsp: 1.676 ± 0.318
1.197HisGlu: 1.197 ± 0.282
0.838HisPhe: 0.838 ± 0.225
2.215HisGly: 2.215 ± 0.428
0.479HisHis: 0.479 ± 0.176
1.078HisIle: 1.078 ± 0.234
0.718HisLys: 0.718 ± 0.201
1.736HisLeu: 1.736 ± 0.358
0.18HisMet: 0.18 ± 0.109
0.299HisAsn: 0.299 ± 0.136
1.257HisPro: 1.257 ± 0.282
0.659HisGln: 0.659 ± 0.192
1.317HisArg: 1.317 ± 0.264
0.838HisSer: 0.838 ± 0.213
1.377HisThr: 1.377 ± 0.36
1.197HisVal: 1.197 ± 0.236
0.479HisTrp: 0.479 ± 0.178
0.539HisTyr: 0.539 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
6.047IleAla: 6.047 ± 0.487
0.359IleCys: 0.359 ± 0.156
2.934IleAsp: 2.934 ± 0.359
3.652IleGlu: 3.652 ± 0.558
1.138IlePhe: 1.138 ± 0.249
3.772IleGly: 3.772 ± 0.43
1.257IleHis: 1.257 ± 0.259
1.616IleIle: 1.616 ± 0.288
1.616IleLys: 1.616 ± 0.346
2.874IleLeu: 2.874 ± 0.376
0.718IleMet: 0.718 ± 0.187
2.215IleAsn: 2.215 ± 0.292
4.131IlePro: 4.131 ± 0.578
1.497IleGln: 1.497 ± 0.443
3.353IleArg: 3.353 ± 0.429
2.155IleSer: 2.155 ± 0.375
3.353IleThr: 3.353 ± 0.526
3.053IleVal: 3.053 ± 0.469
0.898IleTrp: 0.898 ± 0.279
0.958IleTyr: 0.958 ± 0.206
0.0IleXaa: 0.0 ± 0.0
Lys
5.149LysAla: 5.149 ± 0.486
0.239LysCys: 0.239 ± 0.153
2.455LysAsp: 2.455 ± 0.329
1.856LysGlu: 1.856 ± 0.363
1.257LysPhe: 1.257 ± 0.279
5.029LysGly: 5.029 ± 0.647
0.898LysHis: 0.898 ± 0.253
2.515LysIle: 2.515 ± 0.456
2.275LysLys: 2.275 ± 0.388
3.532LysLeu: 3.532 ± 0.418
0.419LysMet: 0.419 ± 0.15
0.718LysAsn: 0.718 ± 0.219
3.113LysPro: 3.113 ± 0.411
1.138LysGln: 1.138 ± 0.238
2.754LysArg: 2.754 ± 0.429
1.257LysSer: 1.257 ± 0.22
2.634LysThr: 2.634 ± 0.455
3.951LysVal: 3.951 ± 0.577
0.838LysTrp: 0.838 ± 0.2
1.078LysTyr: 1.078 ± 0.271
0.0LysXaa: 0.0 ± 0.0
Leu
8.681LeuAla: 8.681 ± 1.025
0.479LeuCys: 0.479 ± 0.202
5.448LeuAsp: 5.448 ± 0.575
4.251LeuGlu: 4.251 ± 0.516
2.215LeuPhe: 2.215 ± 0.279
7.843LeuGly: 7.843 ± 0.955
1.557LeuHis: 1.557 ± 0.302
3.892LeuIle: 3.892 ± 0.455
3.472LeuLys: 3.472 ± 0.454
5.388LeuLeu: 5.388 ± 0.579
2.634LeuMet: 2.634 ± 0.306
3.113LeuAsn: 3.113 ± 0.373
4.011LeuPro: 4.011 ± 0.621
2.934LeuGln: 2.934 ± 0.532
5.328LeuArg: 5.328 ± 0.551
4.67LeuSer: 4.67 ± 0.437
4.79LeuThr: 4.79 ± 0.753
5.089LeuVal: 5.089 ± 0.462
1.377LeuTrp: 1.377 ± 0.337
2.275LeuTyr: 2.275 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
2.155MetAla: 2.155 ± 0.333
0.12MetCys: 0.12 ± 0.094
1.197MetAsp: 1.197 ± 0.266
1.317MetGlu: 1.317 ± 0.279
0.718MetPhe: 0.718 ± 0.176
2.275MetGly: 2.275 ± 0.435
0.419MetHis: 0.419 ± 0.161
0.958MetIle: 0.958 ± 0.203
1.197MetLys: 1.197 ± 0.262
1.616MetLeu: 1.616 ± 0.292
0.479MetMet: 0.479 ± 0.146
0.838MetAsn: 0.838 ± 0.172
1.437MetPro: 1.437 ± 0.273
0.958MetGln: 0.958 ± 0.264
1.736MetArg: 1.736 ± 0.343
2.574MetSer: 2.574 ± 0.363
2.215MetThr: 2.215 ± 0.388
1.437MetVal: 1.437 ± 0.274
0.299MetTrp: 0.299 ± 0.139
0.599MetTyr: 0.599 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
2.574AsnAla: 2.574 ± 0.405
0.12AsnCys: 0.12 ± 0.09
1.736AsnAsp: 1.736 ± 0.333
1.736AsnGlu: 1.736 ± 0.327
1.257AsnPhe: 1.257 ± 0.28
2.993AsnGly: 2.993 ± 0.602
0.599AsnHis: 0.599 ± 0.173
1.317AsnIle: 1.317 ± 0.311
1.377AsnLys: 1.377 ± 0.283
2.694AsnLeu: 2.694 ± 0.447
0.659AsnMet: 0.659 ± 0.182
0.778AsnAsn: 0.778 ± 0.213
2.275AsnPro: 2.275 ± 0.399
0.958AsnGln: 0.958 ± 0.234
2.275AsnArg: 2.275 ± 0.357
1.796AsnSer: 1.796 ± 0.336
2.095AsnThr: 2.095 ± 0.345
2.395AsnVal: 2.395 ± 0.364
0.659AsnTrp: 0.659 ± 0.163
0.718AsnTyr: 0.718 ± 0.153
0.0AsnXaa: 0.0 ± 0.0
Pro
4.61ProAla: 4.61 ± 0.463
0.479ProCys: 0.479 ± 0.163
3.413ProAsp: 3.413 ± 0.455
4.37ProGlu: 4.37 ± 0.63
1.616ProPhe: 1.616 ± 0.315
5.508ProGly: 5.508 ± 0.587
1.018ProHis: 1.018 ± 0.245
2.515ProIle: 2.515 ± 0.344
2.515ProLys: 2.515 ± 0.456
4.55ProLeu: 4.55 ± 0.531
1.018ProMet: 1.018 ± 0.234
1.976ProAsn: 1.976 ± 0.336
2.694ProPro: 2.694 ± 0.52
3.233ProGln: 3.233 ± 0.9
2.934ProArg: 2.934 ± 0.436
3.233ProSer: 3.233 ± 0.453
4.011ProThr: 4.011 ± 0.53
3.712ProVal: 3.712 ± 0.652
1.257ProTrp: 1.257 ± 0.53
1.616ProTyr: 1.616 ± 0.306
0.0ProXaa: 0.0 ± 0.0
Gln
4.071GlnAla: 4.071 ± 0.485
0.239GlnCys: 0.239 ± 0.141
1.736GlnAsp: 1.736 ± 0.323
2.215GlnGlu: 2.215 ± 0.349
1.557GlnPhe: 1.557 ± 0.308
4.311GlnGly: 4.311 ± 1.724
0.718GlnHis: 0.718 ± 0.193
2.215GlnIle: 2.215 ± 0.377
1.437GlnLys: 1.437 ± 0.314
3.173GlnLeu: 3.173 ± 0.656
1.377GlnMet: 1.377 ± 0.324
1.078GlnAsn: 1.078 ± 0.242
1.437GlnPro: 1.437 ± 0.315
1.736GlnGln: 1.736 ± 0.367
2.993GlnArg: 2.993 ± 0.373
1.377GlnSer: 1.377 ± 0.271
2.155GlnThr: 2.155 ± 0.424
2.694GlnVal: 2.694 ± 0.419
0.898GlnTrp: 0.898 ± 0.272
0.958GlnTyr: 0.958 ± 0.221
0.0GlnXaa: 0.0 ± 0.0
Arg
4.79ArgAla: 4.79 ± 0.713
1.018ArgCys: 1.018 ± 0.279
3.832ArgAsp: 3.832 ± 0.499
4.071ArgGlu: 4.071 ± 0.518
2.275ArgPhe: 2.275 ± 0.345
4.67ArgGly: 4.67 ± 0.626
1.497ArgHis: 1.497 ± 0.359
2.934ArgIle: 2.934 ± 0.331
3.413ArgLys: 3.413 ± 0.555
5.328ArgLeu: 5.328 ± 0.576
2.275ArgMet: 2.275 ± 0.359
2.155ArgAsn: 2.155 ± 0.362
2.694ArgPro: 2.694 ± 0.439
2.634ArgGln: 2.634 ± 0.399
4.969ArgArg: 4.969 ± 0.515
2.574ArgSer: 2.574 ± 0.376
2.874ArgThr: 2.874 ± 0.506
4.37ArgVal: 4.37 ± 0.45
1.497ArgTrp: 1.497 ± 0.322
2.455ArgTyr: 2.455 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
4.969SerAla: 4.969 ± 0.677
0.239SerCys: 0.239 ± 0.115
4.071SerAsp: 4.071 ± 0.561
2.874SerGlu: 2.874 ± 0.36
1.976SerPhe: 1.976 ± 0.336
5.927SerGly: 5.927 ± 0.715
0.718SerHis: 0.718 ± 0.173
2.215SerIle: 2.215 ± 0.315
2.215SerLys: 2.215 ± 0.461
4.43SerLeu: 4.43 ± 0.425
1.497SerMet: 1.497 ± 0.288
1.377SerAsn: 1.377 ± 0.295
2.395SerPro: 2.395 ± 0.395
2.395SerGln: 2.395 ± 0.481
4.131SerArg: 4.131 ± 0.694
2.275SerSer: 2.275 ± 0.434
2.874SerThr: 2.874 ± 0.423
2.993SerVal: 2.993 ± 0.47
0.778SerTrp: 0.778 ± 0.225
1.257SerTyr: 1.257 ± 0.318
0.0SerXaa: 0.0 ± 0.0
Thr
6.825ThrAla: 6.825 ± 0.673
0.479ThrCys: 0.479 ± 0.169
3.293ThrAsp: 3.293 ± 0.476
3.293ThrGlu: 3.293 ± 0.392
2.455ThrPhe: 2.455 ± 0.42
6.825ThrGly: 6.825 ± 0.714
0.838ThrHis: 0.838 ± 0.219
2.455ThrIle: 2.455 ± 0.391
2.754ThrLys: 2.754 ± 0.296
5.807ThrLeu: 5.807 ± 0.637
1.497ThrMet: 1.497 ± 0.346
1.856ThrAsn: 1.856 ± 0.331
3.652ThrPro: 3.652 ± 0.467
1.856ThrGln: 1.856 ± 0.292
2.934ThrArg: 2.934 ± 0.382
3.233ThrSer: 3.233 ± 0.474
4.61ThrThr: 4.61 ± 0.79
4.969ThrVal: 4.969 ± 0.583
1.078ThrTrp: 1.078 ± 0.224
1.257ThrTyr: 1.257 ± 0.222
0.0ThrXaa: 0.0 ± 0.0
Val
7.184ValAla: 7.184 ± 0.614
0.718ValCys: 0.718 ± 0.214
4.73ValAsp: 4.73 ± 0.563
5.269ValGlu: 5.269 ± 0.456
2.634ValPhe: 2.634 ± 0.372
5.508ValGly: 5.508 ± 0.673
1.377ValHis: 1.377 ± 0.285
3.472ValIle: 3.472 ± 0.401
3.173ValLys: 3.173 ± 0.485
5.388ValLeu: 5.388 ± 0.535
1.557ValMet: 1.557 ± 0.335
2.395ValAsn: 2.395 ± 0.376
3.772ValPro: 3.772 ± 0.598
2.455ValGln: 2.455 ± 0.312
4.55ValArg: 4.55 ± 0.564
4.311ValSer: 4.311 ± 0.507
5.328ValThr: 5.328 ± 0.578
5.987ValVal: 5.987 ± 0.58
1.257ValTrp: 1.257 ± 0.281
2.515ValTyr: 2.515 ± 0.491
0.0ValXaa: 0.0 ± 0.0
Trp
1.497TrpAla: 1.497 ± 0.275
0.299TrpCys: 0.299 ± 0.129
1.736TrpAsp: 1.736 ± 0.355
0.898TrpGlu: 0.898 ± 0.191
0.659TrpPhe: 0.659 ± 0.193
1.736TrpGly: 1.736 ± 0.323
0.299TrpHis: 0.299 ± 0.145
1.377TrpIle: 1.377 ± 0.262
0.479TrpLys: 0.479 ± 0.173
1.616TrpLeu: 1.616 ± 0.27
0.718TrpMet: 0.718 ± 0.182
0.778TrpAsn: 0.778 ± 0.249
0.958TrpPro: 0.958 ± 0.219
1.078TrpGln: 1.078 ± 0.279
1.317TrpArg: 1.317 ± 0.285
1.377TrpSer: 1.377 ± 0.258
1.317TrpThr: 1.317 ± 0.285
1.197TrpVal: 1.197 ± 0.245
0.539TrpTrp: 0.539 ± 0.241
0.659TrpTyr: 0.659 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.634TyrAla: 2.634 ± 0.379
0.239TyrCys: 0.239 ± 0.119
2.036TyrAsp: 2.036 ± 0.36
1.736TyrGlu: 1.736 ± 0.326
1.138TyrPhe: 1.138 ± 0.271
1.856TyrGly: 1.856 ± 0.312
0.359TyrHis: 0.359 ± 0.13
1.557TyrIle: 1.557 ± 0.305
0.958TyrLys: 0.958 ± 0.253
2.395TyrLeu: 2.395 ± 0.326
0.778TyrMet: 0.778 ± 0.213
1.197TyrAsn: 1.197 ± 0.219
1.317TyrPro: 1.317 ± 0.273
1.377TyrGln: 1.377 ± 0.28
2.155TyrArg: 2.155 ± 0.399
1.437TyrSer: 1.437 ± 0.346
1.616TyrThr: 1.616 ± 0.297
1.916TyrVal: 1.916 ± 0.309
0.359TyrTrp: 0.359 ± 0.156
0.778TyrTyr: 0.778 ± 0.239
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (16704 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski