Nereida ignava

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Nereida

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2850 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1NMZ0|A0A0U1NMZ0_9RHOB Putative phosphonate metabolism protein OS=Nereida ignava OX=282199 GN=NIG5292_02161 PE=4 SV=1
MM1 pKa = 7.93PFTAANGDD9 pKa = 3.44AKK11 pKa = 10.29ATAEE15 pKa = 4.31CRR17 pKa = 11.84TSEE20 pKa = 4.0ITLDD24 pKa = 3.96EE25 pKa = 4.27YY26 pKa = 11.46RR27 pKa = 11.84SLTPKK32 pKa = 9.71MDD34 pKa = 4.25AADD37 pKa = 3.77STATTVAAYY46 pKa = 10.59LGGTAGWRR54 pKa = 11.84TDD56 pKa = 3.43LYY58 pKa = 10.69SAEE61 pKa = 4.38PAMLMTQAEE70 pKa = 4.68SIEE73 pKa = 4.08LFRR76 pKa = 11.84DD77 pKa = 3.85LGAKK81 pKa = 7.63FTPEE85 pKa = 3.8LKK87 pKa = 10.48SPSVEE92 pKa = 3.85MPFNGFTQEE101 pKa = 5.22DD102 pKa = 4.95YY103 pKa = 10.25AQKK106 pKa = 10.22MVDD109 pKa = 3.58EE110 pKa = 4.6YY111 pKa = 11.44VAAGIPASDD120 pKa = 3.33VWLQSFNLEE129 pKa = 4.85DD130 pKa = 3.16VLYY133 pKa = 8.98WIKK136 pKa = 10.6AAPEE140 pKa = 3.94FGAQAVYY147 pKa = 10.99LMDD150 pKa = 4.55EE151 pKa = 4.38YY152 pKa = 11.46NIDD155 pKa = 3.67GYY157 pKa = 11.78SPMDD161 pKa = 3.67ASTWPNTMEE170 pKa = 4.63DD171 pKa = 4.08FKK173 pKa = 11.83AMGVNYY179 pKa = 9.76IAPPTWMLVTLEE191 pKa = 4.2NGEE194 pKa = 4.23IVPSEE199 pKa = 4.15LALQAKK205 pKa = 9.68AADD208 pKa = 4.18LNIITWTIEE217 pKa = 3.67RR218 pKa = 11.84SGPLVNGGGWYY229 pKa = 9.17YY230 pKa = 11.14QSIADD235 pKa = 4.2AVNSDD240 pKa = 2.79GVMYY244 pKa = 10.26EE245 pKa = 4.6LIDD248 pKa = 4.27VLAQDD253 pKa = 3.99VGVDD257 pKa = 4.71GIFSDD262 pKa = 4.1WPATVTYY269 pKa = 9.85YY270 pKa = 11.07ANCMGLEE277 pKa = 4.04

Molecular weight: 30.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1NLJ8|A0A0U1NLJ8_9RHOB D-beta-hydroxybutyrate dehydrogenase OS=Nereida ignava OX=282199 GN=bdhA_2 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2850

0

2850

861993

29

1509

302.5

32.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.83 ± 0.057

0.929 ± 0.014

6.148 ± 0.04

5.698 ± 0.037

3.832 ± 0.03

8.281 ± 0.046

2.025 ± 0.021

5.52 ± 0.036

3.822 ± 0.041

9.679 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.025

3.031 ± 0.027

4.624 ± 0.025

3.464 ± 0.023

5.985 ± 0.04

5.568 ± 0.037

5.779 ± 0.032

7.351 ± 0.037

1.321 ± 0.021

2.244 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski