Sporomusa sphaeroides DSM 2875

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Sporomusa; Sporomusa sphaeroides

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4565 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7MHL0|A0A1U7MHL0_9FIRM Germination-specific N-acetylmuramoyl-L-alanine amidase OS=Sporomusa sphaeroides DSM 2875 OX=1337886 GN=cwlD PE=4 SV=1
MM1 pKa = 7.57GSTFGGYY8 pKa = 9.91SIAYY12 pKa = 9.08SGMYY16 pKa = 10.24ANQSALTVTSQNLSNVNTSGYY37 pKa = 10.41SRR39 pKa = 11.84QQINSAEE46 pKa = 4.17KK47 pKa = 9.62IVPISSQTSIGAGVAVVEE65 pKa = 4.48VCRR68 pKa = 11.84ARR70 pKa = 11.84NQLLDD75 pKa = 3.56STYY78 pKa = 10.57HH79 pKa = 6.92DD80 pKa = 4.33KK81 pKa = 11.17NASASYY87 pKa = 9.24WSTKK91 pKa = 10.66SGMLTYY97 pKa = 10.39AQEE100 pKa = 4.2ILDD103 pKa = 4.4EE104 pKa = 4.98FSATDD109 pKa = 3.8GSGSDD114 pKa = 4.09GLQQTIQNFFDD125 pKa = 3.39SWEE128 pKa = 4.08EE129 pKa = 3.75LAGDD133 pKa = 4.65AGNIANRR140 pKa = 11.84QAVVEE145 pKa = 4.13YY146 pKa = 10.49AATLLDD152 pKa = 3.81TLSSIDD158 pKa = 3.62EE159 pKa = 4.04QLAALQQDD167 pKa = 4.12ACNSAADD174 pKa = 3.62IVDD177 pKa = 5.22DD178 pKa = 4.39INSIANQVAALNLQIVRR195 pKa = 11.84AEE197 pKa = 4.0AADD200 pKa = 3.97AEE202 pKa = 4.42ASDD205 pKa = 5.64LRR207 pKa = 11.84DD208 pKa = 3.37QRR210 pKa = 11.84DD211 pKa = 3.47YY212 pKa = 11.88LLDD215 pKa = 3.59QLSDD219 pKa = 3.57YY220 pKa = 11.7VNFSTQEE227 pKa = 3.75QANGSVTVFIGGVALVSLNKK247 pKa = 10.27SYY249 pKa = 10.79EE250 pKa = 3.85LSLAGDD256 pKa = 4.61GSTADD261 pKa = 3.63PLVVRR266 pKa = 11.84WADD269 pKa = 3.04TGTRR273 pKa = 11.84ASITNGTLKK282 pKa = 10.81ACLEE286 pKa = 4.61DD287 pKa = 4.61ADD289 pKa = 3.98QTGIGAIDD297 pKa = 3.93PEE299 pKa = 5.45LILDD303 pKa = 4.05TDD305 pKa = 3.45QDD307 pKa = 4.11GKK309 pKa = 11.38VYY311 pKa = 9.97TYY313 pKa = 8.78TASATSSLSNLRR325 pKa = 11.84QGLNTLITTLAVKK338 pKa = 10.53LNSLHH343 pKa = 6.61SAGTGLDD350 pKa = 3.47GSTGTDD356 pKa = 3.43FFVAIDD362 pKa = 3.72SSQPLSSTNIQVNPAIVSDD381 pKa = 4.23LNKK384 pKa = 10.16LAAGASGAAGDD395 pKa = 4.0NTVAAAICDD404 pKa = 3.86LSSAGIYY411 pKa = 9.72TFDD414 pKa = 4.8GLSQDD419 pKa = 2.89GTAFYY424 pKa = 10.82QSLISWLSTAGSDD437 pKa = 4.18ASDD440 pKa = 4.27YY441 pKa = 11.48YY442 pKa = 10.71DD443 pKa = 3.6TQSALATQVDD453 pKa = 3.95NQRR456 pKa = 11.84QSISTVSLDD465 pKa = 3.52EE466 pKa = 4.53EE467 pKa = 4.47MSNMITFQNAYY478 pKa = 9.12AASARR483 pKa = 11.84VLSVMDD489 pKa = 4.45GLLADD494 pKa = 5.86LIAEE498 pKa = 4.74LGG500 pKa = 3.57

Molecular weight: 52.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7MJN6|A0A1U7MJN6_9FIRM Demethylrebeccamycin-D-glucose O-methyltransferase OS=Sporomusa sphaeroides DSM 2875 OX=1337886 GN=rebM_2 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84FEE4 pKa = 4.33MLLIIAGMAAVTFFTRR20 pKa = 11.84FGALALLKK28 pKa = 9.87KK29 pKa = 9.19TGLPCWFEE37 pKa = 3.2RR38 pKa = 11.84WLRR41 pKa = 11.84HH42 pKa = 5.27VPTAVLTALIVPALLLPQGRR62 pKa = 11.84VDD64 pKa = 4.54LSLSNHH70 pKa = 5.76YY71 pKa = 10.53LLAGILAAIVAYY83 pKa = 7.67TCRR86 pKa = 11.84NAMLTMGLGLTAMLSLRR103 pKa = 11.84WLGII107 pKa = 3.56

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4565

0

4565

1425425

29

5143

312.3

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.758 ± 0.045

1.233 ± 0.014

4.851 ± 0.026

6.173 ± 0.034

3.766 ± 0.027

7.667 ± 0.043

1.828 ± 0.015

7.19 ± 0.036

5.581 ± 0.031

9.963 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.021

3.938 ± 0.036

3.982 ± 0.024

3.929 ± 0.027

4.742 ± 0.026

5.407 ± 0.027

5.622 ± 0.033

7.451 ± 0.028

0.972 ± 0.014

3.267 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski