Streptococcus phage Javan436

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B2V6|A0A4D6B2V6_9VIRU Uncharacterized protein OS=Streptococcus phage Javan436 OX=2548165 GN=Javan436_0012 PE=4 SV=1
MM1 pKa = 7.8GILEE5 pKa = 5.46LIEE8 pKa = 3.97QFEE11 pKa = 4.14IDD13 pKa = 4.75YY14 pKa = 11.2YY15 pKa = 9.94PLSYY19 pKa = 10.56EE20 pKa = 4.36KK21 pKa = 9.77KK22 pKa = 8.76TLLADD27 pKa = 3.68QPIHH31 pKa = 6.21QVVACLSEE39 pKa = 3.92MASWHH44 pKa = 5.66EE45 pKa = 4.05CGGRR49 pKa = 11.84LSWW52 pKa = 4.31

Molecular weight: 6.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B481|A0A4D6B481_9VIRU Uncharacterized protein OS=Streptococcus phage Javan436 OX=2548165 GN=Javan436_0016 PE=4 SV=1
MM1 pKa = 7.66SFRR4 pKa = 11.84KK5 pKa = 9.22MKK7 pKa = 10.96AEE9 pKa = 4.04DD10 pKa = 3.42KK11 pKa = 11.05KK12 pKa = 10.53IGRR15 pKa = 11.84WKK17 pKa = 10.59YY18 pKa = 8.58RR19 pKa = 11.84LGWLVVALIWALFIWHH35 pKa = 6.96WFFF38 pKa = 5.71

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29

0

29

3614

38

381

124.6

14.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.281 ± 0.524

0.775 ± 0.144

6.143 ± 0.315

8.329 ± 0.536

4.095 ± 0.41

5.285 ± 0.555

1.799 ± 0.178

7.194 ± 0.568

9.408 ± 0.594

9.353 ± 0.526

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.341

5.23 ± 0.47

2.49 ± 0.353

3.874 ± 0.315

4.787 ± 0.416

5.285 ± 0.367

5.589 ± 0.404

5.562 ± 0.407

1.467 ± 0.352

4.317 ± 0.517
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski