Bodo saltans (Flagellated protozoan)

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Eubodonida; Bodonidae; Bodo

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S4JHA6|A0A0S4JHA6_BODSA Membrane-associated protein putative OS=Bodo saltans OX=75058 GN=BSAL_20505 PE=3 SV=1
MM1 pKa = 7.56AGVPNAAFTIASVPLVILRR20 pKa = 11.84TVDD23 pKa = 3.5EE24 pKa = 4.63SQCTASRR31 pKa = 11.84VVAWCDD37 pKa = 3.13ILATASTMAGVPNAAFTVASVPLVILKK64 pKa = 8.9TEE66 pKa = 4.23FAIQHH71 pKa = 5.16TASGVVAWCDD81 pKa = 3.25ILATASTMAGVANVAFTVASLTLVMLEE108 pKa = 4.49TDD110 pKa = 4.51DD111 pKa = 4.9EE112 pKa = 4.69SHH114 pKa = 6.0STDD117 pKa = 3.02SGVVAWCDD125 pKa = 3.25ILATASTMAGDD136 pKa = 3.74PNAAFTVASVPLVILQTADD155 pKa = 3.42EE156 pKa = 4.37SHH158 pKa = 6.02STDD161 pKa = 3.04SGVVALCDD169 pKa = 3.33ILAAASTMAGDD180 pKa = 3.72PNAAFTVASVPLVILQTQVEE200 pKa = 4.43SHH202 pKa = 5.46RR203 pKa = 11.84TASGVVAWCDD213 pKa = 3.25ILATASTMAGVANVAFTVASVTLVMLEE240 pKa = 4.13TDD242 pKa = 5.3DD243 pKa = 5.72EE244 pKa = 4.84SQCTASGVVAWCDD257 pKa = 3.25ILATASTMAGVANVAFIDD275 pKa = 3.66TSTRR279 pKa = 11.84PKK281 pKa = 10.17MSS283 pKa = 3.06

Molecular weight: 28.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S4II91|A0A0S4II91_BODSA Uncharacterized protein OS=Bodo saltans OX=75058 GN=BSAL_52915 PE=4 SV=1
II1 pKa = 8.23SSMGTVSRR9 pKa = 11.84PLGMRR14 pKa = 11.84PKK16 pKa = 8.74WHH18 pKa = 6.93KK19 pKa = 10.7KK20 pKa = 8.61RR21 pKa = 11.84IKK23 pKa = 9.87RR24 pKa = 11.84LKK26 pKa = 9.0LRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84MRR33 pKa = 11.84QRR35 pKa = 11.84SKK37 pKa = 11.39

Molecular weight: 4.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18124

0

18124

9801806

9

10369

540.8

58.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.535 ± 0.02

1.765 ± 0.014

4.978 ± 0.014

5.121 ± 0.021

3.562 ± 0.012

6.079 ± 0.016

2.773 ± 0.01

4.13 ± 0.012

3.566 ± 0.02

9.205 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.007

3.685 ± 0.012

5.417 ± 0.018

4.294 ± 0.016

6.033 ± 0.02

9.854 ± 0.046

6.884 ± 0.028

7.323 ± 0.017

1.125 ± 0.006

2.135 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski