Streptomyces sp. TSRI0281

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4ZG98|A0A1Q4ZG98_9ACTN 4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase OS=Streptomyces sp. TSRI0281 OX=1718998 GN=A6A29_30145 PE=4 SV=1
MM1 pKa = 7.36SCCTPDD7 pKa = 4.13GSCSTDD13 pKa = 2.9TVTVDD18 pKa = 3.61VIEE21 pKa = 4.66GTTTVYY27 pKa = 10.53NVSGMTCGHH36 pKa = 6.74CKK38 pKa = 9.42ATLTKK43 pKa = 10.44EE44 pKa = 3.52IGALDD49 pKa = 4.07GVLMVDD55 pKa = 3.45VDD57 pKa = 4.14VEE59 pKa = 4.18AGRR62 pKa = 11.84VSVTTSGEE70 pKa = 3.76PDD72 pKa = 3.37DD73 pKa = 5.52ALLAKK78 pKa = 10.5VVDD81 pKa = 4.06DD82 pKa = 5.92AGYY85 pKa = 10.19EE86 pKa = 4.16LNGRR90 pKa = 11.84AAA92 pKa = 4.13

Molecular weight: 9.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4YAH6|A0A1Q4YAH6_9ACTN ABC transporter OS=Streptomyces sp. TSRI0281 OX=1718998 GN=A6A29_23275 PE=4 SV=1
MM1 pKa = 7.19VVTTGPVPVRR11 pKa = 11.84LDD13 pKa = 3.19VTTTVAVSAVTTAGLRR29 pKa = 11.84AAAAPVAASVAMTTVAVPRR48 pKa = 11.84AVVSVVTTIAVAVAPVVVSAVMTTVAVAPVVVSAVMTTVAVAPVVVSVVTTTVAVRR104 pKa = 11.84RR105 pKa = 11.84AVVSVVTTAVGRR117 pKa = 11.84VVMTTVVGVPAVGSSATTVVGVPVVVLSVVMIGRR151 pKa = 11.84VGRR154 pKa = 11.84VAMTTAPPVAASVVTTAGLRR174 pKa = 11.84AAVAPVVVSVAMTTVAVRR192 pKa = 11.84RR193 pKa = 11.84AVVSVVTTAVGRR205 pKa = 11.84VAMTTVVGVPAVGSSATTVVGVPVVVLSVVMIGRR239 pKa = 11.84VGRR242 pKa = 11.84VAMTTAPPAVASVATTTVAVAPVVASVAMTTVAVRR277 pKa = 11.84RR278 pKa = 11.84AVVSVVTTAVGRR290 pKa = 11.84VVMTTVVGVPVVVLSVVMIGRR311 pKa = 11.84VGRR314 pKa = 11.84VAMTTAPPAVASVATTTVAVAPVVASVVTTTVVAVPRR351 pKa = 11.84AVVSVVTTAVGRR363 pKa = 11.84VVMTTVVGVPAVGSSATTVVGVPVVVLSVVMIGRR397 pKa = 11.84VGRR400 pKa = 11.84VAMTTAPPAVVSAVTTTVAVAPVVASVVTTTVAVAPAVPRR440 pKa = 11.84AAVSVVTTGTVIVGRR455 pKa = 11.84VVMTTVVGTAVGSSATTAVASSVVTTGTVTGIAIRR490 pKa = 11.84SSGFRR495 pKa = 11.84FPMTSPATRR504 pKa = 11.84STRR507 pKa = 11.84MCGRR511 pKa = 11.84SS512 pKa = 3.06

Molecular weight: 49.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7837

0

7837

2581200

29

6782

329.4

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.46 ± 0.039

0.797 ± 0.008

6.033 ± 0.024

5.624 ± 0.027

2.722 ± 0.017

9.56 ± 0.027

2.305 ± 0.014

3.304 ± 0.019

2.172 ± 0.026

10.32 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.758 ± 0.012

1.783 ± 0.015

6.14 ± 0.024

2.741 ± 0.017

7.99 ± 0.034

5.255 ± 0.022

6.237 ± 0.024

8.254 ± 0.03

1.484 ± 0.011

2.061 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski