Amino acid dipepetide frequency for Streptomyces sp. TSRI0281

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.987AlaAla: 20.987 ± 0.148
1.062AlaCys: 1.062 ± 0.02
8.308AlaAsp: 8.308 ± 0.058
8.588AlaGlu: 8.588 ± 0.079
3.457AlaPhe: 3.457 ± 0.037
13.157AlaGly: 13.157 ± 0.087
2.914AlaHis: 2.914 ± 0.035
3.456AlaIle: 3.456 ± 0.045
2.853AlaLys: 2.853 ± 0.042
14.516AlaLeu: 14.516 ± 0.106
2.545AlaMet: 2.545 ± 0.037
1.908AlaAsn: 1.908 ± 0.033
7.431AlaPro: 7.431 ± 0.074
3.701AlaGln: 3.701 ± 0.042
10.051AlaArg: 10.051 ± 0.082
5.891AlaSer: 5.891 ± 0.056
7.094AlaThr: 7.094 ± 0.071
12.185AlaVal: 12.185 ± 0.08
1.755AlaTrp: 1.755 ± 0.028
2.746AlaTyr: 2.746 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.107CysAla: 1.107 ± 0.021
0.099CysCys: 0.099 ± 0.005
0.48CysAsp: 0.48 ± 0.014
0.413CysGlu: 0.413 ± 0.015
0.224CysPhe: 0.224 ± 0.009
0.976CysGly: 0.976 ± 0.02
0.207CysHis: 0.207 ± 0.009
0.181CysIle: 0.181 ± 0.007
0.106CysLys: 0.106 ± 0.006
0.728CysLeu: 0.728 ± 0.016
0.126CysMet: 0.126 ± 0.008
0.125CysAsn: 0.125 ± 0.007
0.489CysPro: 0.489 ± 0.015
0.175CysGln: 0.175 ± 0.008
0.631CysArg: 0.631 ± 0.015
0.438CysSer: 0.438 ± 0.014
0.525CysThr: 0.525 ± 0.016
0.667CysVal: 0.667 ± 0.018
0.127CysTrp: 0.127 ± 0.007
0.148CysTyr: 0.148 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.721AspAla: 7.721 ± 0.064
0.42AspCys: 0.42 ± 0.015
3.587AspAsp: 3.587 ± 0.044
3.872AspGlu: 3.872 ± 0.041
1.671AspPhe: 1.671 ± 0.028
6.46AspGly: 6.46 ± 0.061
1.43AspHis: 1.43 ± 0.025
2.049AspIle: 2.049 ± 0.036
1.199AspLys: 1.199 ± 0.024
6.179AspLeu: 6.179 ± 0.056
0.814AspMet: 0.814 ± 0.017
0.985AspAsn: 0.985 ± 0.02
4.527AspPro: 4.527 ± 0.045
1.506AspGln: 1.506 ± 0.024
5.181AspArg: 5.181 ± 0.053
2.824AspSer: 2.824 ± 0.033
3.338AspThr: 3.338 ± 0.038
4.472AspVal: 4.472 ± 0.044
1.0AspTrp: 1.0 ± 0.02
1.094AspTyr: 1.094 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
7.101GluAla: 7.101 ± 0.071
0.392GluCys: 0.392 ± 0.012
2.851GluAsp: 2.851 ± 0.036
3.455GluGlu: 3.455 ± 0.048
1.459GluPhe: 1.459 ± 0.023
4.312GluGly: 4.312 ± 0.041
1.493GluHis: 1.493 ± 0.027
2.304GluIle: 2.304 ± 0.037
1.479GluLys: 1.479 ± 0.029
6.794GluLeu: 6.794 ± 0.058
0.869GluMet: 0.869 ± 0.019
1.046GluAsn: 1.046 ± 0.024
3.353GluPro: 3.353 ± 0.042
2.262GluGln: 2.262 ± 0.034
5.362GluArg: 5.362 ± 0.054
2.718GluSer: 2.718 ± 0.035
2.832GluThr: 2.832 ± 0.034
4.261GluVal: 4.261 ± 0.048
0.785GluTrp: 0.785 ± 0.02
1.115GluTyr: 1.115 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
3.648PheAla: 3.648 ± 0.039
0.279PheCys: 0.279 ± 0.011
2.005PheAsp: 2.005 ± 0.032
1.416PheGlu: 1.416 ± 0.023
0.867PhePhe: 0.867 ± 0.02
3.016PheGly: 3.016 ± 0.039
0.63PheHis: 0.63 ± 0.016
0.791PheIle: 0.791 ± 0.02
0.485PheLys: 0.485 ± 0.015
2.573PheLeu: 2.573 ± 0.029
0.405PheMet: 0.405 ± 0.013
0.551PheAsn: 0.551 ± 0.015
1.387PhePro: 1.387 ± 0.024
0.64PheGln: 0.64 ± 0.016
1.88PheArg: 1.88 ± 0.032
1.501PheSer: 1.501 ± 0.026
2.091PheThr: 2.091 ± 0.033
2.115PheVal: 2.115 ± 0.029
0.393PheTrp: 0.393 ± 0.013
0.551PheTyr: 0.551 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.403GlyAla: 11.403 ± 0.082
0.855GlyCys: 0.855 ± 0.018
5.279GlyAsp: 5.279 ± 0.054
5.166GlyGlu: 5.166 ± 0.052
2.894GlyPhe: 2.894 ± 0.034
8.997GlyGly: 8.997 ± 0.097
2.339GlyHis: 2.339 ± 0.031
3.665GlyIle: 3.665 ± 0.041
2.484GlyLys: 2.484 ± 0.041
9.381GlyLeu: 9.381 ± 0.078
2.01GlyMet: 2.01 ± 0.029
1.803GlyAsn: 1.803 ± 0.034
5.397GlyPro: 5.397 ± 0.053
2.716GlyGln: 2.716 ± 0.036
7.856GlyArg: 7.856 ± 0.065
5.785GlySer: 5.785 ± 0.059
6.509GlyThr: 6.509 ± 0.06
7.134GlyVal: 7.134 ± 0.063
1.686GlyTrp: 1.686 ± 0.03
2.244GlyTyr: 2.244 ± 0.031
0.0GlyXaa: 0.0 ± 0.0
His
2.653HisAla: 2.653 ± 0.033
0.215HisCys: 0.215 ± 0.011
1.369HisAsp: 1.369 ± 0.027
1.197HisGlu: 1.197 ± 0.023
0.64HisPhe: 0.64 ± 0.017
2.4HisGly: 2.4 ± 0.03
0.71HisHis: 0.71 ± 0.018
0.726HisIle: 0.726 ± 0.019
0.349HisLys: 0.349 ± 0.011
2.47HisLeu: 2.47 ± 0.032
0.336HisMet: 0.336 ± 0.011
0.373HisAsn: 0.373 ± 0.012
1.805HisPro: 1.805 ± 0.029
0.665HisGln: 0.665 ± 0.015
2.248HisArg: 2.248 ± 0.033
1.103HisSer: 1.103 ± 0.023
1.361HisThr: 1.361 ± 0.025
1.585HisVal: 1.585 ± 0.025
0.374HisTrp: 0.374 ± 0.011
0.476HisTyr: 0.476 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.933IleAla: 4.933 ± 0.046
0.318IleCys: 0.318 ± 0.013
2.405IleAsp: 2.405 ± 0.027
2.072IleGlu: 2.072 ± 0.033
0.712IlePhe: 0.712 ± 0.017
3.821IleGly: 3.821 ± 0.039
0.625IleHis: 0.625 ± 0.015
0.966IleIle: 0.966 ± 0.022
0.766IleLys: 0.766 ± 0.02
2.476IleLeu: 2.476 ± 0.035
0.461IleMet: 0.461 ± 0.012
0.733IleAsn: 0.733 ± 0.018
1.845IlePro: 1.845 ± 0.028
0.756IleGln: 0.756 ± 0.018
2.337IleArg: 2.337 ± 0.031
1.822IleSer: 1.822 ± 0.032
2.352IleThr: 2.352 ± 0.035
2.717IleVal: 2.717 ± 0.041
0.397IleTrp: 0.397 ± 0.013
0.526IleTyr: 0.526 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
2.873LysAla: 2.873 ± 0.037
0.111LysCys: 0.111 ± 0.007
1.378LysAsp: 1.378 ± 0.029
1.214LysGlu: 1.214 ± 0.021
0.461LysPhe: 0.461 ± 0.016
1.871LysGly: 1.871 ± 0.036
0.433LysHis: 0.433 ± 0.015
0.878LysIle: 0.878 ± 0.021
0.915LysLys: 0.915 ± 0.027
2.063LysLeu: 2.063 ± 0.031
0.385LysMet: 0.385 ± 0.013
0.581LysAsn: 0.581 ± 0.019
1.383LysPro: 1.383 ± 0.027
0.697LysGln: 0.697 ± 0.019
1.449LysArg: 1.449 ± 0.022
1.22LysSer: 1.22 ± 0.026
1.236LysThr: 1.236 ± 0.025
1.886LysVal: 1.886 ± 0.033
0.244LysTrp: 0.244 ± 0.01
0.439LysTyr: 0.439 ± 0.012
0.0LysXaa: 0.0 ± 0.0
Leu
14.799LeuAla: 14.799 ± 0.091
0.892LeuCys: 0.892 ± 0.019
6.839LeuAsp: 6.839 ± 0.063
4.48LeuGlu: 4.48 ± 0.047
2.629LeuPhe: 2.629 ± 0.036
9.247LeuGly: 9.247 ± 0.067
2.345LeuHis: 2.345 ± 0.034
3.575LeuIle: 3.575 ± 0.043
2.066LeuLys: 2.066 ± 0.033
11.302LeuLeu: 11.302 ± 0.103
1.693LeuMet: 1.693 ± 0.026
1.726LeuAsn: 1.726 ± 0.028
6.59LeuPro: 6.59 ± 0.054
2.12LeuGln: 2.12 ± 0.032
8.664LeuArg: 8.664 ± 0.065
5.552LeuSer: 5.552 ± 0.05
7.004LeuThr: 7.004 ± 0.053
8.561LeuVal: 8.561 ± 0.062
1.288LeuTrp: 1.288 ± 0.023
1.827LeuTyr: 1.827 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.262MetAla: 2.262 ± 0.032
0.143MetCys: 0.143 ± 0.007
0.917MetAsp: 0.917 ± 0.02
0.812MetGlu: 0.812 ± 0.018
0.467MetPhe: 0.467 ± 0.013
1.305MetGly: 1.305 ± 0.022
0.354MetHis: 0.354 ± 0.011
0.676MetIle: 0.676 ± 0.016
0.428MetLys: 0.428 ± 0.013
1.73MetLeu: 1.73 ± 0.028
0.31MetMet: 0.31 ± 0.011
0.455MetAsn: 0.455 ± 0.012
1.127MetPro: 1.127 ± 0.023
0.441MetGln: 0.441 ± 0.014
1.438MetArg: 1.438 ± 0.024
1.326MetSer: 1.326 ± 0.024
1.517MetThr: 1.517 ± 0.026
1.328MetVal: 1.328 ± 0.023
0.206MetTrp: 0.206 ± 0.009
0.333MetTyr: 0.333 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.204AsnAla: 2.204 ± 0.032
0.167AsnCys: 0.167 ± 0.009
1.0AsnAsp: 1.0 ± 0.021
0.819AsnGlu: 0.819 ± 0.018
0.488AsnPhe: 0.488 ± 0.013
1.991AsnGly: 1.991 ± 0.034
0.396AsnHis: 0.396 ± 0.012
0.649AsnIle: 0.649 ± 0.015
0.418AsnLys: 0.418 ± 0.015
1.648AsnLeu: 1.648 ± 0.025
0.312AsnMet: 0.312 ± 0.01
0.445AsnAsn: 0.445 ± 0.015
1.327AsnPro: 1.327 ± 0.027
0.489AsnGln: 0.489 ± 0.014
1.33AsnArg: 1.33 ± 0.023
0.955AsnSer: 0.955 ± 0.023
1.177AsnThr: 1.177 ± 0.026
1.32AsnVal: 1.32 ± 0.021
0.291AsnTrp: 0.291 ± 0.012
0.399AsnTyr: 0.399 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.775ProAla: 8.775 ± 0.078
0.328ProCys: 0.328 ± 0.014
4.596ProAsp: 4.596 ± 0.049
4.242ProGlu: 4.242 ± 0.047
1.538ProPhe: 1.538 ± 0.027
6.801ProGly: 6.801 ± 0.06
1.426ProHis: 1.426 ± 0.023
1.258ProIle: 1.258 ± 0.023
1.261ProLys: 1.261 ± 0.027
5.467ProLeu: 5.467 ± 0.049
0.988ProMet: 0.988 ± 0.02
0.876ProAsn: 0.876 ± 0.021
3.557ProPro: 3.557 ± 0.063
1.812ProGln: 1.812 ± 0.035
3.846ProArg: 3.846 ± 0.043
3.322ProSer: 3.322 ± 0.034
3.228ProThr: 3.228 ± 0.036
5.733ProVal: 5.733 ± 0.054
0.896ProTrp: 0.896 ± 0.024
1.451ProTyr: 1.451 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.525GlnAla: 3.525 ± 0.042
0.181GlnCys: 0.181 ± 0.008
1.454GlnAsp: 1.454 ± 0.024
1.518GlnGlu: 1.518 ± 0.026
0.704GlnPhe: 0.704 ± 0.015
2.251GlnGly: 2.251 ± 0.031
0.678GlnHis: 0.678 ± 0.018
1.09GlnIle: 1.09 ± 0.021
0.594GlnLys: 0.594 ± 0.017
3.093GlnLeu: 3.093 ± 0.038
0.488GlnMet: 0.488 ± 0.015
0.502GlnAsn: 0.502 ± 0.014
1.696GlnPro: 1.696 ± 0.035
1.326GlnGln: 1.326 ± 0.042
2.362GlnArg: 2.362 ± 0.031
1.299GlnSer: 1.299 ± 0.021
1.301GlnThr: 1.301 ± 0.023
2.285GlnVal: 2.285 ± 0.031
0.478GlnTrp: 0.478 ± 0.015
0.584GlnTyr: 0.584 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
9.738ArgAla: 9.738 ± 0.074
0.583ArgCys: 0.583 ± 0.017
4.33ArgAsp: 4.33 ± 0.044
4.647ArgGlu: 4.647 ± 0.042
2.291ArgPhe: 2.291 ± 0.031
5.861ArgGly: 5.861 ± 0.055
2.049ArgHis: 2.049 ± 0.029
3.473ArgIle: 3.473 ± 0.039
1.664ArgLys: 1.664 ± 0.026
8.646ArgLeu: 8.646 ± 0.073
1.764ArgMet: 1.764 ± 0.027
1.439ArgAsn: 1.439 ± 0.024
4.907ArgPro: 4.907 ± 0.053
2.259ArgGln: 2.259 ± 0.03
7.428ArgArg: 7.428 ± 0.069
4.261ArgSer: 4.261 ± 0.043
5.848ArgThr: 5.848 ± 0.056
5.604ArgVal: 5.604 ± 0.053
1.336ArgTrp: 1.336 ± 0.025
1.77ArgTyr: 1.77 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
7.068SerAla: 7.068 ± 0.061
0.431SerCys: 0.431 ± 0.013
2.848SerAsp: 2.848 ± 0.038
2.468SerGlu: 2.468 ± 0.034
1.608SerPhe: 1.608 ± 0.023
6.397SerGly: 6.397 ± 0.064
1.081SerHis: 1.081 ± 0.024
1.526SerIle: 1.526 ± 0.029
1.051SerLys: 1.051 ± 0.024
4.997SerLeu: 4.997 ± 0.048
1.12SerMet: 1.12 ± 0.021
0.89SerAsn: 0.89 ± 0.021
3.295SerPro: 3.295 ± 0.035
1.237SerGln: 1.237 ± 0.024
3.728SerArg: 3.728 ± 0.037
2.976SerSer: 2.976 ± 0.041
3.213SerThr: 3.213 ± 0.038
4.468SerVal: 4.468 ± 0.043
0.902SerTrp: 0.902 ± 0.021
1.249SerTyr: 1.249 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
9.165ThrAla: 9.165 ± 0.068
0.421ThrCys: 0.421 ± 0.015
3.718ThrAsp: 3.718 ± 0.037
3.359ThrGlu: 3.359 ± 0.041
1.665ThrPhe: 1.665 ± 0.032
7.092ThrGly: 7.092 ± 0.057
1.241ThrHis: 1.241 ± 0.028
1.691ThrIle: 1.691 ± 0.029
1.153ThrLys: 1.153 ± 0.026
5.865ThrLeu: 5.865 ± 0.052
0.973ThrMet: 0.973 ± 0.018
0.992ThrAsn: 0.992 ± 0.022
4.136ThrPro: 4.136 ± 0.044
1.342ThrGln: 1.342 ± 0.026
3.87ThrArg: 3.87 ± 0.04
3.235ThrSer: 3.235 ± 0.041
3.906ThrThr: 3.906 ± 0.05
6.243ThrVal: 6.243 ± 0.06
0.908ThrTrp: 0.908 ± 0.025
1.394ThrTyr: 1.394 ± 0.024
0.0ThrXaa: 0.0 ± 0.0
Val
10.276ValAla: 10.276 ± 0.067
0.73ValCys: 0.73 ± 0.018
4.749ValAsp: 4.749 ± 0.048
4.529ValGlu: 4.529 ± 0.047
2.328ValPhe: 2.328 ± 0.032
6.306ValGly: 6.306 ± 0.054
1.961ValHis: 1.961 ± 0.031
3.013ValIle: 3.013 ± 0.034
1.693ValLys: 1.693 ± 0.034
9.444ValLeu: 9.444 ± 0.075
1.438ValMet: 1.438 ± 0.028
1.614ValAsn: 1.614 ± 0.027
5.273ValPro: 5.273 ± 0.053
2.07ValGln: 2.07 ± 0.031
7.108ValArg: 7.108 ± 0.056
4.422ValSer: 4.422 ± 0.048
5.579ValThr: 5.579 ± 0.053
7.42ValVal: 7.42 ± 0.072
1.087ValTrp: 1.087 ± 0.023
1.502ValTyr: 1.502 ± 0.023
0.0ValXaa: 0.0 ± 0.0
Trp
1.609TrpAla: 1.609 ± 0.024
0.161TrpCys: 0.161 ± 0.008
0.808TrpAsp: 0.808 ± 0.022
0.716TrpGlu: 0.716 ± 0.017
0.499TrpPhe: 0.499 ± 0.016
1.042TrpGly: 1.042 ± 0.02
0.384TrpHis: 0.384 ± 0.014
0.558TrpIle: 0.558 ± 0.016
0.374TrpLys: 0.374 ± 0.013
1.722TrpLeu: 1.722 ± 0.032
0.291TrpMet: 0.291 ± 0.012
0.399TrpAsn: 0.399 ± 0.015
0.779TrpPro: 0.779 ± 0.018
0.621TrpGln: 0.621 ± 0.015
1.258TrpArg: 1.258 ± 0.026
0.954TrpSer: 0.954 ± 0.019
1.025TrpThr: 1.025 ± 0.02
0.967TrpVal: 0.967 ± 0.021
0.334TrpTrp: 0.334 ± 0.012
0.337TrpTyr: 0.337 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.759TyrAla: 2.759 ± 0.033
0.184TyrCys: 0.184 ± 0.008
1.409TyrAsp: 1.409 ± 0.024
1.26TyrGlu: 1.26 ± 0.02
0.622TyrPhe: 0.622 ± 0.014
2.3TyrGly: 2.3 ± 0.029
0.359TyrHis: 0.359 ± 0.012
0.511TyrIle: 0.511 ± 0.016
0.379TyrLys: 0.379 ± 0.013
2.105TyrLeu: 2.105 ± 0.033
0.25TyrMet: 0.25 ± 0.011
0.384TyrAsn: 0.384 ± 0.013
1.096TyrPro: 1.096 ± 0.02
0.573TyrGln: 0.573 ± 0.017
1.87TyrArg: 1.87 ± 0.027
0.949TyrSer: 0.949 ± 0.019
1.23TyrThr: 1.23 ± 0.023
1.592TyrVal: 1.592 ± 0.026
0.351TyrTrp: 0.351 ± 0.01
0.422TyrTyr: 0.422 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7837 proteins (2581200 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski