Amino acid dipepetide frequency for Enterococcus phage vB_EfaS-DELF1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.794AlaAla: 2.794 ± 0.825
0.164AlaCys: 0.164 ± 0.119
2.466AlaAsp: 2.466 ± 0.508
3.781AlaGlu: 3.781 ± 0.508
2.794AlaPhe: 2.794 ± 0.58
3.37AlaGly: 3.37 ± 0.613
0.822AlaHis: 0.822 ± 0.33
4.685AlaIle: 4.685 ± 0.861
4.274AlaLys: 4.274 ± 0.643
5.178AlaLeu: 5.178 ± 1.044
1.89AlaMet: 1.89 ± 0.451
3.452AlaAsn: 3.452 ± 0.515
2.137AlaPro: 2.137 ± 0.451
2.383AlaGln: 2.383 ± 0.592
2.301AlaArg: 2.301 ± 0.488
3.699AlaSer: 3.699 ± 0.679
3.37AlaThr: 3.37 ± 0.54
3.123AlaVal: 3.123 ± 0.478
0.575AlaTrp: 0.575 ± 0.167
2.301AlaTyr: 2.301 ± 0.361
0.0AlaXaa: 0.0 ± 0.0
Cys
0.164CysAla: 0.164 ± 0.176
0.082CysCys: 0.082 ± 0.087
0.411CysAsp: 0.411 ± 0.162
0.329CysGlu: 0.329 ± 0.194
0.164CysPhe: 0.164 ± 0.099
0.411CysGly: 0.411 ± 0.171
0.082CysHis: 0.082 ± 0.088
0.575CysIle: 0.575 ± 0.184
0.658CysLys: 0.658 ± 0.21
0.493CysLeu: 0.493 ± 0.247
0.082CysMet: 0.082 ± 0.09
0.164CysAsn: 0.164 ± 0.123
0.164CysPro: 0.164 ± 0.109
0.082CysGln: 0.082 ± 0.087
0.247CysArg: 0.247 ± 0.154
0.247CysSer: 0.247 ± 0.15
0.493CysThr: 0.493 ± 0.19
0.411CysVal: 0.411 ± 0.227
0.164CysTrp: 0.164 ± 0.112
0.329CysTyr: 0.329 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
3.452AspAla: 3.452 ± 0.632
0.329AspCys: 0.329 ± 0.166
2.466AspAsp: 2.466 ± 0.511
4.767AspGlu: 4.767 ± 0.685
3.041AspPhe: 3.041 ± 0.418
4.931AspGly: 4.931 ± 0.633
0.74AspHis: 0.74 ± 0.262
3.699AspIle: 3.699 ± 0.526
5.671AspLys: 5.671 ± 0.554
6.329AspLeu: 6.329 ± 0.718
1.397AspMet: 1.397 ± 0.36
3.288AspAsn: 3.288 ± 0.585
1.89AspPro: 1.89 ± 0.389
0.986AspGln: 0.986 ± 0.268
2.548AspArg: 2.548 ± 0.447
2.712AspSer: 2.712 ± 0.417
4.192AspThr: 4.192 ± 0.454
3.863AspVal: 3.863 ± 0.509
0.658AspTrp: 0.658 ± 0.219
3.288AspTyr: 3.288 ± 0.471
0.0AspXaa: 0.0 ± 0.0
Glu
3.945GluAla: 3.945 ± 0.542
0.329GluCys: 0.329 ± 0.162
4.685GluAsp: 4.685 ± 0.658
7.233GluGlu: 7.233 ± 1.086
3.863GluPhe: 3.863 ± 0.56
4.438GluGly: 4.438 ± 0.568
1.233GluHis: 1.233 ± 0.371
4.849GluIle: 4.849 ± 0.633
5.096GluLys: 5.096 ± 0.799
7.233GluLeu: 7.233 ± 1.005
2.548GluMet: 2.548 ± 0.452
4.027GluAsn: 4.027 ± 0.583
1.89GluPro: 1.89 ± 0.475
2.877GluGln: 2.877 ± 0.515
2.63GluArg: 2.63 ± 0.628
4.52GluSer: 4.52 ± 0.609
3.452GluThr: 3.452 ± 0.566
6.246GluVal: 6.246 ± 0.943
1.068GluTrp: 1.068 ± 0.206
3.041GluTyr: 3.041 ± 0.61
0.0GluXaa: 0.0 ± 0.0
Phe
1.89PheAla: 1.89 ± 0.324
0.411PheCys: 0.411 ± 0.179
3.205PheAsp: 3.205 ± 0.447
3.452PheGlu: 3.452 ± 0.506
0.904PhePhe: 0.904 ± 0.245
1.89PheGly: 1.89 ± 0.526
0.247PheHis: 0.247 ± 0.144
2.959PheIle: 2.959 ± 0.425
3.945PheLys: 3.945 ± 0.486
2.301PheLeu: 2.301 ± 0.389
1.808PheMet: 1.808 ± 0.402
3.452PheAsn: 3.452 ± 0.745
0.575PhePro: 0.575 ± 0.252
1.726PheGln: 1.726 ± 0.32
1.562PheArg: 1.562 ± 0.333
2.959PheSer: 2.959 ± 0.446
2.712PheThr: 2.712 ± 0.34
2.794PheVal: 2.794 ± 0.35
0.411PheTrp: 0.411 ± 0.152
1.562PheTyr: 1.562 ± 0.416
0.0PheXaa: 0.0 ± 0.0
Gly
3.452GlyAla: 3.452 ± 0.803
0.247GlyCys: 0.247 ± 0.131
3.288GlyAsp: 3.288 ± 0.538
3.616GlyGlu: 3.616 ± 0.536
3.781GlyPhe: 3.781 ± 0.463
4.603GlyGly: 4.603 ± 0.891
0.822GlyHis: 0.822 ± 0.256
4.109GlyIle: 4.109 ± 0.727
7.561GlyLys: 7.561 ± 1.05
6.329GlyLeu: 6.329 ± 1.033
1.562GlyMet: 1.562 ± 0.327
3.781GlyAsn: 3.781 ± 0.671
0.411GlyPro: 0.411 ± 0.167
1.233GlyGln: 1.233 ± 0.311
2.877GlyArg: 2.877 ± 0.44
3.123GlySer: 3.123 ± 0.584
5.425GlyThr: 5.425 ± 0.859
3.863GlyVal: 3.863 ± 0.636
1.068GlyTrp: 1.068 ± 0.287
4.274GlyTyr: 4.274 ± 0.623
0.0GlyXaa: 0.0 ± 0.0
His
0.74HisAla: 0.74 ± 0.286
0.0HisCys: 0.0 ± 0.0
0.658HisAsp: 0.658 ± 0.22
0.74HisGlu: 0.74 ± 0.245
0.74HisPhe: 0.74 ± 0.276
1.315HisGly: 1.315 ± 0.299
0.247HisHis: 0.247 ± 0.139
1.644HisIle: 1.644 ± 0.274
1.315HisLys: 1.315 ± 0.389
0.658HisLeu: 0.658 ± 0.247
0.164HisMet: 0.164 ± 0.112
1.233HisAsn: 1.233 ± 0.269
0.575HisPro: 0.575 ± 0.181
0.575HisGln: 0.575 ± 0.184
0.575HisArg: 0.575 ± 0.294
0.493HisSer: 0.493 ± 0.198
1.151HisThr: 1.151 ± 0.296
0.986HisVal: 0.986 ± 0.262
0.247HisTrp: 0.247 ± 0.137
0.74HisTyr: 0.74 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
4.274IleAla: 4.274 ± 0.545
0.493IleCys: 0.493 ± 0.236
5.753IleAsp: 5.753 ± 0.754
4.109IleGlu: 4.109 ± 0.636
1.726IlePhe: 1.726 ± 0.445
4.109IleGly: 4.109 ± 0.633
0.822IleHis: 0.822 ± 0.292
4.603IleIle: 4.603 ± 0.552
5.671IleLys: 5.671 ± 0.672
4.356IleLeu: 4.356 ± 0.607
1.726IleMet: 1.726 ± 0.401
5.425IleAsn: 5.425 ± 0.571
2.548IlePro: 2.548 ± 0.442
2.301IleGln: 2.301 ± 0.416
2.548IleArg: 2.548 ± 0.387
4.027IleSer: 4.027 ± 0.483
4.438IleThr: 4.438 ± 0.593
3.781IleVal: 3.781 ± 0.446
1.068IleTrp: 1.068 ± 0.289
2.383IleTyr: 2.383 ± 0.468
0.0IleXaa: 0.0 ± 0.0
Lys
6.246LysAla: 6.246 ± 1.16
0.247LysCys: 0.247 ± 0.142
5.178LysAsp: 5.178 ± 0.647
8.466LysGlu: 8.466 ± 0.977
2.794LysPhe: 2.794 ± 0.495
5.918LysGly: 5.918 ± 0.824
1.315LysHis: 1.315 ± 0.346
4.356LysIle: 4.356 ± 0.535
6.986LysLys: 6.986 ± 1.007
8.548LysLeu: 8.548 ± 0.946
2.466LysMet: 2.466 ± 0.523
4.685LysAsn: 4.685 ± 0.57
2.466LysPro: 2.466 ± 0.382
4.274LysGln: 4.274 ± 0.682
4.109LysArg: 4.109 ± 0.523
4.027LysSer: 4.027 ± 0.583
5.753LysThr: 5.753 ± 0.439
5.835LysVal: 5.835 ± 0.733
0.658LysTrp: 0.658 ± 0.272
4.109LysTyr: 4.109 ± 0.496
0.0LysXaa: 0.0 ± 0.0
Leu
5.014LeuAla: 5.014 ± 0.858
0.411LeuCys: 0.411 ± 0.187
7.068LeuAsp: 7.068 ± 0.655
7.479LeuGlu: 7.479 ± 0.947
2.219LeuPhe: 2.219 ± 0.348
5.918LeuGly: 5.918 ± 0.933
1.068LeuHis: 1.068 ± 0.296
5.26LeuIle: 5.26 ± 0.817
8.055LeuLys: 8.055 ± 0.981
7.479LeuLeu: 7.479 ± 0.952
2.055LeuMet: 2.055 ± 0.314
6.329LeuAsn: 6.329 ± 0.655
3.205LeuPro: 3.205 ± 0.691
2.794LeuGln: 2.794 ± 0.421
3.205LeuArg: 3.205 ± 0.483
4.931LeuSer: 4.931 ± 0.626
6.164LeuThr: 6.164 ± 0.62
4.356LeuVal: 4.356 ± 0.532
0.74LeuTrp: 0.74 ± 0.26
3.123LeuTyr: 3.123 ± 0.529
0.0LeuXaa: 0.0 ± 0.0
Met
1.726MetAla: 1.726 ± 0.355
0.247MetCys: 0.247 ± 0.126
1.151MetAsp: 1.151 ± 0.344
1.808MetGlu: 1.808 ± 0.509
1.068MetPhe: 1.068 ± 0.255
1.151MetGly: 1.151 ± 0.343
0.329MetHis: 0.329 ± 0.161
1.89MetIle: 1.89 ± 0.447
3.781MetLys: 3.781 ± 0.496
1.808MetLeu: 1.808 ± 0.371
1.068MetMet: 1.068 ± 0.314
2.63MetAsn: 2.63 ± 0.593
0.822MetPro: 0.822 ± 0.276
1.068MetGln: 1.068 ± 0.294
0.658MetArg: 0.658 ± 0.202
0.986MetSer: 0.986 ± 0.26
1.315MetThr: 1.315 ± 0.284
1.315MetVal: 1.315 ± 0.348
0.247MetTrp: 0.247 ± 0.138
1.151MetTyr: 1.151 ± 0.349
0.0MetXaa: 0.0 ± 0.0
Asn
3.945AsnAla: 3.945 ± 0.537
0.247AsnCys: 0.247 ± 0.149
3.534AsnAsp: 3.534 ± 0.484
4.356AsnGlu: 4.356 ± 0.629
2.466AsnPhe: 2.466 ± 0.355
6.74AsnGly: 6.74 ± 0.784
1.397AsnHis: 1.397 ± 0.385
4.603AsnIle: 4.603 ± 0.64
6.246AsnLys: 6.246 ± 0.849
5.014AsnLeu: 5.014 ± 0.6
1.726AsnMet: 1.726 ± 0.346
5.096AsnAsn: 5.096 ± 0.558
3.123AsnPro: 3.123 ± 0.5
1.89AsnGln: 1.89 ± 0.34
2.383AsnArg: 2.383 ± 0.443
4.027AsnSer: 4.027 ± 0.512
4.438AsnThr: 4.438 ± 0.764
4.767AsnVal: 4.767 ± 0.75
0.74AsnTrp: 0.74 ± 0.187
3.041AsnTyr: 3.041 ± 0.588
0.0AsnXaa: 0.0 ± 0.0
Pro
1.562ProAla: 1.562 ± 0.328
0.164ProCys: 0.164 ± 0.121
1.644ProAsp: 1.644 ± 0.277
3.37ProGlu: 3.37 ± 0.851
1.315ProPhe: 1.315 ± 0.295
0.493ProGly: 0.493 ± 0.301
0.904ProHis: 0.904 ± 0.288
1.233ProIle: 1.233 ± 0.272
2.877ProLys: 2.877 ± 0.465
2.712ProLeu: 2.712 ± 0.396
0.74ProMet: 0.74 ± 0.273
1.89ProAsn: 1.89 ± 0.388
0.575ProPro: 0.575 ± 0.245
1.479ProGln: 1.479 ± 0.282
0.658ProArg: 0.658 ± 0.237
1.644ProSer: 1.644 ± 0.425
1.89ProThr: 1.89 ± 0.409
1.973ProVal: 1.973 ± 0.335
0.247ProTrp: 0.247 ± 0.131
1.644ProTyr: 1.644 ± 0.44
0.0ProXaa: 0.0 ± 0.0
Gln
2.137GlnAla: 2.137 ± 0.326
0.247GlnCys: 0.247 ± 0.144
1.644GlnAsp: 1.644 ± 0.372
2.219GlnGlu: 2.219 ± 0.606
1.973GlnPhe: 1.973 ± 0.472
1.397GlnGly: 1.397 ± 0.382
0.329GlnHis: 0.329 ± 0.149
2.959GlnIle: 2.959 ± 0.476
1.808GlnLys: 1.808 ± 0.417
4.356GlnLeu: 4.356 ± 0.737
1.233GlnMet: 1.233 ± 0.31
1.973GlnAsn: 1.973 ± 0.459
1.068GlnPro: 1.068 ± 0.389
1.726GlnGln: 1.726 ± 0.37
1.068GlnArg: 1.068 ± 0.283
1.808GlnSer: 1.808 ± 0.295
2.301GlnThr: 2.301 ± 0.336
3.041GlnVal: 3.041 ± 0.472
0.493GlnTrp: 0.493 ± 0.22
1.89GlnTyr: 1.89 ± 0.353
0.0GlnXaa: 0.0 ± 0.0
Arg
1.315ArgAla: 1.315 ± 0.317
0.247ArgCys: 0.247 ± 0.187
2.794ArgAsp: 2.794 ± 0.611
2.466ArgGlu: 2.466 ± 0.681
1.89ArgPhe: 1.89 ± 0.418
2.219ArgGly: 2.219 ± 0.407
0.822ArgHis: 0.822 ± 0.259
2.219ArgIle: 2.219 ± 0.585
2.877ArgLys: 2.877 ± 0.536
3.781ArgLeu: 3.781 ± 0.579
1.068ArgMet: 1.068 ± 0.284
2.466ArgAsn: 2.466 ± 0.353
0.904ArgPro: 0.904 ± 0.207
1.808ArgGln: 1.808 ± 0.351
1.644ArgArg: 1.644 ± 0.43
2.137ArgSer: 2.137 ± 0.373
2.548ArgThr: 2.548 ± 0.488
2.466ArgVal: 2.466 ± 0.548
0.493ArgTrp: 0.493 ± 0.163
2.219ArgTyr: 2.219 ± 0.376
0.0ArgXaa: 0.0 ± 0.0
Ser
3.123SerAla: 3.123 ± 0.682
0.329SerCys: 0.329 ± 0.18
3.37SerAsp: 3.37 ± 0.528
3.616SerGlu: 3.616 ± 0.521
2.301SerPhe: 2.301 ± 0.404
4.192SerGly: 4.192 ± 0.88
0.986SerHis: 0.986 ± 0.267
3.452SerIle: 3.452 ± 0.424
5.096SerLys: 5.096 ± 0.615
4.931SerLeu: 4.931 ± 0.474
1.397SerMet: 1.397 ± 0.316
4.603SerAsn: 4.603 ± 0.611
1.233SerPro: 1.233 ± 0.242
2.301SerGln: 2.301 ± 0.345
1.89SerArg: 1.89 ± 0.324
3.123SerSer: 3.123 ± 0.571
3.616SerThr: 3.616 ± 0.568
3.123SerVal: 3.123 ± 0.579
0.74SerTrp: 0.74 ± 0.217
1.89SerTyr: 1.89 ± 0.421
0.0SerXaa: 0.0 ± 0.0
Thr
3.288ThrAla: 3.288 ± 0.792
0.247ThrCys: 0.247 ± 0.158
2.794ThrAsp: 2.794 ± 0.618
4.109ThrGlu: 4.109 ± 0.496
2.548ThrPhe: 2.548 ± 0.358
5.178ThrGly: 5.178 ± 0.654
0.822ThrHis: 0.822 ± 0.236
4.931ThrIle: 4.931 ± 0.635
6.329ThrLys: 6.329 ± 0.679
4.931ThrLeu: 4.931 ± 0.554
0.986ThrMet: 0.986 ± 0.285
6.493ThrAsn: 6.493 ± 0.708
2.794ThrPro: 2.794 ± 0.449
2.301ThrGln: 2.301 ± 0.464
1.726ThrArg: 1.726 ± 0.359
3.37ThrSer: 3.37 ± 0.672
3.452ThrThr: 3.452 ± 0.581
4.274ThrVal: 4.274 ± 0.71
1.233ThrTrp: 1.233 ± 0.289
2.219ThrTyr: 2.219 ± 0.479
0.0ThrXaa: 0.0 ± 0.0
Val
3.863ValAla: 3.863 ± 0.451
0.74ValCys: 0.74 ± 0.321
3.863ValAsp: 3.863 ± 0.49
4.767ValGlu: 4.767 ± 0.711
2.877ValPhe: 2.877 ± 0.552
4.027ValGly: 4.027 ± 0.848
0.822ValHis: 0.822 ± 0.235
3.616ValIle: 3.616 ± 0.497
5.753ValLys: 5.753 ± 0.83
5.918ValLeu: 5.918 ± 0.652
0.658ValMet: 0.658 ± 0.248
5.096ValAsn: 5.096 ± 0.808
1.562ValPro: 1.562 ± 0.334
2.383ValGln: 2.383 ± 0.411
2.794ValArg: 2.794 ± 0.462
4.52ValSer: 4.52 ± 0.536
3.534ValThr: 3.534 ± 0.462
4.931ValVal: 4.931 ± 0.501
1.068ValTrp: 1.068 ± 0.35
2.712ValTyr: 2.712 ± 0.493
0.0ValXaa: 0.0 ± 0.0
Trp
0.164TrpAla: 0.164 ± 0.109
0.082TrpCys: 0.082 ± 0.086
1.151TrpAsp: 1.151 ± 0.452
0.986TrpGlu: 0.986 ± 0.311
0.575TrpPhe: 0.575 ± 0.222
0.411TrpGly: 0.411 ± 0.191
0.247TrpHis: 0.247 ± 0.139
0.74TrpIle: 0.74 ± 0.27
0.822TrpLys: 0.822 ± 0.263
1.315TrpLeu: 1.315 ± 0.385
0.329TrpMet: 0.329 ± 0.153
0.575TrpAsn: 0.575 ± 0.257
0.082TrpPro: 0.082 ± 0.075
0.411TrpGln: 0.411 ± 0.172
1.151TrpArg: 1.151 ± 0.288
0.904TrpSer: 0.904 ± 0.272
0.74TrpThr: 0.74 ± 0.233
0.986TrpVal: 0.986 ± 0.225
0.082TrpTrp: 0.082 ± 0.084
0.575TrpTyr: 0.575 ± 0.252
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.466TyrAla: 2.466 ± 0.557
0.493TyrCys: 0.493 ± 0.208
3.123TyrAsp: 3.123 ± 0.464
3.534TyrGlu: 3.534 ± 0.573
1.726TyrPhe: 1.726 ± 0.315
2.712TyrGly: 2.712 ± 0.422
0.74TyrHis: 0.74 ± 0.258
3.781TyrIle: 3.781 ± 0.615
3.616TyrLys: 3.616 ± 0.561
3.288TyrLeu: 3.288 ± 0.652
1.233TyrMet: 1.233 ± 0.317
3.123TyrAsn: 3.123 ± 0.494
0.986TyrPro: 0.986 ± 0.238
1.068TyrGln: 1.068 ± 0.324
1.808TyrArg: 1.808 ± 0.306
2.055TyrSer: 2.055 ± 0.449
3.041TyrThr: 3.041 ± 0.524
3.37TyrVal: 3.37 ± 0.61
0.329TyrTrp: 0.329 ± 0.161
2.055TyrTyr: 2.055 ± 0.507
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12168 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski